FastQCFastQC Report
Thu 26 May 2016
SRR938281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2258738
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83730.37069372366339076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71190.31517599650778444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44840.1985179334655015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.056.2091871
GTATCAA73950.043.0401151
ATCAACG95300.033.2966963
TATCAAC96200.032.937272
TCAACGC96850.032.760914
CAACGCA99250.032.0165675
AACGCAG102200.031.092416
TGGTATC17400.030.304742
ACGCAGA117650.026.726697
CGCAGAG118300.026.5798448
GTGGTAT21600.025.2918681
TACATGG74500.024.0393052
GCAGAGT130350.023.685449
GTACATG78300.023.6613581
GTACGGG1652.4295077E-623.0325951
GAGTACT80800.022.57300812-13
GGACCGA4850.022.5250236
ACATGGG79200.022.3118383
GTACTTT84100.021.63078514-15
CAGAGTA124150.021.30973410-11
Produced by FastQC (version 0.11.5)