##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938281_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2258738 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48391491177817 34.0 31.0 34.0 31.0 34.0 2 32.70541470502555 34.0 31.0 34.0 31.0 34.0 3 32.76562354730827 34.0 31.0 34.0 31.0 34.0 4 36.22733668092537 37.0 35.0 37.0 35.0 37.0 5 36.18559744423656 37.0 35.0 37.0 35.0 37.0 6 36.22310909897474 37.0 37.0 37.0 35.0 37.0 7 36.188190485129304 37.0 37.0 37.0 35.0 37.0 8 36.17996509555336 37.0 37.0 37.0 35.0 37.0 9 37.89207557494495 39.0 38.0 39.0 35.0 39.0 10-11 37.87536358798586 39.0 38.0 39.0 35.0 39.0 12-13 37.82351715869658 39.0 38.0 39.0 35.0 39.0 14-15 39.14497453887967 40.0 38.5 41.0 36.0 41.0 16-17 39.09561378964714 40.0 38.0 41.0 36.0 41.0 18-19 39.116194751228335 40.0 38.5 41.0 36.0 41.0 20-21 39.04406952023652 40.0 39.0 41.0 35.5 41.0 22-23 38.898990055508875 40.0 38.0 41.0 35.0 41.0 24-25 38.86401698647652 40.0 38.0 41.0 35.0 41.0 26-27 38.83260497676136 40.0 38.0 41.0 35.0 41.0 28-29 38.77732521434535 40.0 38.0 41.0 35.0 41.0 30-31 38.59563393363905 40.0 38.0 41.0 34.5 41.0 32-33 38.43438836199684 40.0 38.0 41.0 34.0 41.0 34-35 38.24089779336957 40.0 38.0 41.0 34.0 41.0 36-37 38.1140196428271 40.0 38.0 41.0 33.5 41.0 38-39 38.11820162409275 40.0 38.0 41.0 34.0 41.0 40-41 38.066882259031374 40.0 38.0 41.0 33.0 41.0 42-43 37.92212709043723 40.0 38.0 41.0 33.0 41.0 44-45 37.723568647625356 40.0 37.0 41.0 33.0 41.0 46-47 37.415501045273956 40.0 37.0 41.0 32.5 41.0 48-49 37.09330320736624 40.0 37.0 41.0 31.5 41.0 50-51 36.812917655788326 40.0 36.0 41.0 31.0 41.0 52-53 36.40482406547373 39.0 35.5 41.0 30.0 41.0 54-55 36.19080477682671 39.0 35.0 41.0 30.0 41.0 56-57 35.946687043827126 39.0 35.0 40.5 29.0 41.0 58-59 35.61510188432656 38.5 35.0 40.0 28.5 41.0 60-61 35.25965804798963 38.0 34.5 40.0 28.0 41.0 62-63 34.89771699949264 37.5 34.0 40.0 27.5 41.0 64-65 34.51186547532295 37.0 34.0 40.0 26.5 41.0 66-67 34.084056008266565 36.5 34.0 39.0 26.0 41.0 68-69 33.61995326593877 36.0 33.0 39.0 26.0 40.5 70-71 33.100162790018146 35.0 32.5 38.5 24.5 40.0 72-73 32.67884433697047 35.0 32.0 37.5 24.0 40.0 74-75 32.219477646367125 35.0 32.0 37.0 22.5 39.0 76-77 30.908911746293725 34.0 30.5 35.5 21.5 38.5 78-79 31.63763880538602 35.0 31.5 36.0 23.0 38.5 80-81 31.477546753983862 35.0 32.0 36.0 22.0 37.5 82-83 31.101866838916244 34.5 31.0 35.0 20.0 37.0 84-85 30.707210619381264 34.0 31.0 35.0 19.0 36.5 86-87 30.264648666644824 34.0 31.0 35.0 17.0 36.0 88-89 29.801848421552215 34.0 30.0 35.0 11.0 36.0 90-91 29.446130981105377 34.0 30.0 35.0 7.0 35.0 92-93 29.030039561914663 34.0 29.0 35.0 2.0 35.0 94-95 28.595944283932 34.0 29.0 35.0 2.0 35.0 96-97 27.99924161190895 34.0 29.0 35.0 2.0 35.0 98-99 27.282506868879878 33.0 27.0 35.0 2.0 35.0 100-101 24.800403588198364 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20245737308077594 1101 2 0.1526611866772427 1101 3 0.19689187512761208 1101 4 0.0855075642742591 1101 5 0.1460069015281249 1101 6 0.15663645323193975 1101 7 0.08456498469867313 1101 8 0.07046361927126554 1101 9 0.11795555489518961 1101 10-11 0.151689154701252 1101 12-13 0.17262614635772167 1101 14-15 0.2192945114101974 1101 16-17 0.33170413607697924 1101 18-19 0.311967492051231 1101 20-21 0.2565902937358615 1101 22-23 0.2563879306439176 1101 24-25 0.20006637743214384 1101 26-27 0.14346175905159697 1101 28-29 0.17844336317325826 1101 30-31 0.23708810454377272 1101 32-33 0.2759260780946491 1101 34-35 0.18203400451833573 1101 36-37 0.3220158103620605 1101 38-39 0.3185389566803849 1101 40-41 0.4265689075473844 1101 42-43 0.43230518416985575 1101 44-45 0.46054790195564266 1101 46-47 0.3590170576219265 1101 48-49 0.4634417796579413 1101 50-51 0.4057681066807248 1101 52-53 0.4112834052425143 1101 54-55 0.44561902275364673 1101 56-57 0.4074162526967626 1101 58-59 0.39775260522331024 1101 60-61 0.350632312173083 1101 62-63 0.2722540557314943 1101 64-65 0.28278739078142934 1101 66-67 0.2485952724890339 1101 68-69 0.25066008988854094 1101 70-71 0.2566962989728907 1101 72-73 0.16321533943881406 1101 74-75 0.28754430064794434 1101 76-77 0.25778491280745186 1101 78-79 0.24824245106114873 1101 80-81 0.337202867022544 1101 82-83 0.5625609045194437 1101 84-85 0.47431903488138616 1101 86-87 0.5569226142310022 1101 88-89 0.6902996031318231 1101 90-91 0.7232794253600083 1101 92-93 0.8152718283518787 1101 94-95 0.6875821715493231 1101 96-97 0.7100066205521998 1101 98-99 0.7490049086203641 1101 100-101 0.8975314185352907 1102 1 0.14552454803808956 1102 2 0.10946649404689879 1102 3 0.10947740886950896 1102 4 0.06812057072090028 1102 5 0.1124887332079112 1102 6 0.06067133847160022 1102 7 0.12489803923311626 1102 8 0.10818668897833561 1102 9 0.07080405406490797 1102 10-11 0.15503451888535835 1102 12-13 0.1465037138162586 1102 14-15 0.13491935357064477 1102 16-17 0.17794309898960137 1102 18-19 0.2287669386365465 1102 20-21 0.18227103140257128 1102 22-23 0.2638644357741029 1102 24-25 0.16293973264663464 1102 26-27 0.159138355898385 1102 28-29 0.22742085210975205 1102 30-31 0.21738138406647067 1102 32-33 0.170827351469093 1102 34-35 0.22144822424078825 1102 36-37 0.33808891129771723 1102 38-39 0.33036421274481853 1102 40-41 0.35876526206831727 1102 42-43 0.3713078584586569 1102 44-45 0.45233557245852296 1102 46-47 0.4051767419672885 1102 48-49 0.5558856399523293 1102 50-51 0.5878193322499428 1102 52-53 0.525324389346963 1102 54-55 0.5305365367753652 1102 56-57 0.6785034504430456 1102 58-59 0.6721467850239051 1102 60-61 0.6380785299090164 1102 62-63 0.5960846412380931 1102 64-65 0.7373428378286704 1102 66-67 0.6697883047863158 1102 68-69 0.7574538381182379 1102 70-71 0.8047610719876488 1102 72-73 0.7365695971517923 1102 74-75 0.6204767910429538 1102 76-77 0.8264611120376273 1102 78-79 0.8432111879820212 1102 80-81 1.0403084152094735 1102 82-83 1.0869036300155699 1102 84-85 0.8448140575535383 1102 86-87 0.6982515125926767 1102 88-89 0.4428754890525717 1102 90-91 0.38327349782413833 1102 92-93 0.3265141210533322 1102 94-95 0.3510059376305996 1102 96-97 0.4366451981749968 1102 98-99 0.5375791159626324 1102 100-101 0.914499879994846 1103 1 0.15171479357442053 1103 2 0.12003226267744793 1103 3 0.16207749667798055 1103 4 0.10352749080092138 1103 5 0.10357608920747197 1103 6 0.031825289559087366 1103 7 0.07073381052478567 1103 8 0.09756677458739915 1103 9 0.017879511428688488 1103 10-11 -0.0124558419100822 1103 12-13 0.08945821710656077 1103 14-15 0.18600577356209413 1103 16-17 0.1770565146300882 1103 18-19 0.16747960429528774 1103 20-21 0.14425409679134304 1103 22-23 0.2482955359236172 1103 24-25 0.1927614545825449 1103 26-27 0.1190201250628391 1103 28-29 0.1297082166718937 1103 30-31 0.15848045066071847 1103 32-33 0.15420701650410962 1103 34-35 0.13959714858698646 1103 36-37 0.21031016346668707 1103 38-39 0.14744105103535787 1103 40-41 0.14685523049588767 1103 42-43 0.20305788301312333 1103 44-45 0.16332355209971894 1103 46-47 0.08803841256127498 1103 48-49 0.23170143607548965 1103 50-51 0.13513055452542488 1103 52-53 0.17993907720295965 1103 54-55 0.3697900514228394 1103 56-57 0.38433782401857286 1103 58-59 0.2497045006268621 1103 60-61 0.2253900376040079 1103 62-63 0.26147055779989614 1103 64-65 0.2467352384502064 1103 66-67 0.17304271682632333 1103 68-69 0.34359470607896725 1103 70-71 0.12158502019575934 1103 72-73 -0.013645202017805502 1103 74-75 -0.009244803209703889 1103 76-77 0.006856317325613759 1103 78-79 0.09539349297963184 1103 80-81 0.094599928428174 1103 82-83 0.19390425709902814 1103 84-85 0.2451895311190775 1103 86-87 0.21421097546673096 1103 88-89 0.07849036662858566 1103 90-91 0.14685065217481252 1103 92-93 0.3528710389927028 1103 94-95 0.32916043185463195 1103 96-97 0.5185686780432661 1103 98-99 0.6725058387002427 1103 100-101 0.9313233664949223 1104 1 0.09433743741423939 1104 2 0.0694760674353887 1104 3 0.06257094066601354 1104 4 -0.015424383967499011 1104 5 0.023550909021729183 1104 6 0.007168429352944372 1104 7 0.02202529109580098 1104 8 0.0273931428635521 1104 9 -0.02145038486241191 1104 10-11 -0.032001064647154465 1104 12-13 0.042099746085682455 1104 14-15 0.10266623150680232 1104 16-17 0.036494402841221074 1104 18-19 0.052019089774852034 1104 20-21 0.12028919366732538 1104 22-23 0.08598523396059932 1104 24-25 -0.001045096249946198 1104 26-27 0.10404747664117764 1104 28-29 0.09420485200941897 1104 30-31 0.05653894678209781 1104 32-33 0.0773981867054303 1104 34-35 0.20150902988950747 1104 36-37 0.13682127342680417 1104 38-39 0.1793032492815101 1104 40-41 0.2277112173639395 1104 42-43 0.2802500930923699 1104 44-45 0.2502003723841071 1104 46-47 0.3084970146625423 1104 48-49 0.11342578077311316 1104 50-51 0.1767208904338844 1104 52-53 0.18471733618435593 1104 54-55 0.30227936378862097 1104 56-57 0.41262798183299765 1104 58-59 0.41216490757372526 1104 60-61 0.47591097261761917 1104 62-63 0.4251652719529915 1104 64-65 0.3815815078383551 1104 66-67 0.47689006593446237 1104 68-69 0.49935288067486994 1104 70-71 0.5173146479513377 1104 72-73 0.42517601069499733 1104 74-75 0.38993674114846044 1104 76-77 0.43347759059835766 1104 78-79 0.48151759703117136 1104 80-81 0.5976045182341849 1104 82-83 0.5721359466815201 1104 84-85 0.5996356068747808 1104 86-87 0.5438709035119622 1104 88-89 0.339915577094299 1104 90-91 0.2885121061711722 1104 92-93 0.44234606000939536 1104 94-95 0.4436223049895247 1104 96-97 0.5061695282729559 1104 98-99 0.7842027720564211 1104 100-101 0.8815308440538807 1105 1 0.1675566602081915 1105 2 0.1657273154194172 1105 3 0.0929708715468962 1105 4 0.08954547241834376 1105 5 0.1105613152885283 1105 6 0.1145939343064839 1105 7 0.005242785911867998 1105 8 -0.0014439280219420425 1105 9 0.003040821650145631 1105 10-11 0.06705382745022348 1105 12-13 0.1301258193519459 1105 14-15 0.1834592773558157 1105 16-17 0.1367929590197008 1105 18-19 0.1617543251638338 1105 20-21 0.19390441139253767 1105 22-23 0.1621136995162331 1105 24-25 0.20674424404649727 1105 26-27 0.1768246250936798 1105 28-29 0.06437179387256009 1105 30-31 0.21774152338910824 1105 32-33 0.29893359105225414 1105 34-35 0.3481145133393184 1105 36-37 0.23344067669845003 1105 38-39 0.27004992772402403 1105 40-41 0.35484479005421576 1105 42-43 0.2870093665736704 1105 44-45 0.3836650640360588 1105 46-47 0.33301366084947404 1105 48-49 0.46779228488185964 1105 50-51 0.43295649061819574 1105 52-53 0.3542007667964384 1105 54-55 0.4494277202896555 1105 56-57 0.3968837033597623 1105 58-59 0.44514432543717675 1105 60-61 0.3920920454998864 1105 62-63 0.4056300020044574 1105 64-65 0.36281806801039096 1105 66-67 0.34155483822537036 1105 68-69 0.5102554611218508 1105 70-71 0.5843097904135064 1105 72-73 0.5393435093510206 1105 74-75 0.4980183977032553 1105 76-77 0.5038984492113698 1105 78-79 0.5658333177899451 1105 80-81 0.5129000024519854 1105 82-83 0.6118847178972509 1105 84-85 0.6771550946253377 1105 86-87 0.8193004980131526 1105 88-89 0.5566784025301352 1105 90-91 0.7550171749062891 1105 92-93 0.7350405535198767 1105 94-95 0.619185337782703 1105 96-97 0.8743286558361234 1105 98-99 0.8845416477188088 1105 100-101 0.8564012457127355 1106 1 -0.09805020956658694 1106 2 -0.02203896645259107 1106 3 -0.035706124079531776 1106 4 0.0013872057794444004 1106 5 0.021089490588281024 1106 6 -0.024453178245956053 1106 7 0.012745537987079558 1106 8 -0.1302763787077268 1106 9 -0.14846315026380807 1106 10-11 -0.07168218903247237 1106 12-13 -0.15614244103899466 1106 14-15 -0.26469292727957594 1106 16-17 -0.14017477475793072 1106 18-19 -0.11754225928768136 1106 20-21 -0.10182485134883024 1106 22-23 -0.07445412798070805 1106 24-25 1.6975950274655816E-4 1106 26-27 -0.04619964523448772 1106 28-29 0.11579227377497858 1106 30-31 0.15222930268755874 1106 32-33 0.07028709139440537 1106 34-35 0.023350299242736128 1106 36-37 0.0808130027226639 1106 38-39 0.043256987912656086 1106 40-41 0.0970171815331895 1106 42-43 0.029863268570039736 1106 44-45 0.10916545825223523 1106 46-47 0.10199795914467558 1106 48-49 0.1826953300158891 1106 50-51 0.19029616364191781 1106 52-53 0.10679794480364535 1106 54-55 -0.24824956542037313 1106 56-57 -0.027107140411622765 1106 58-59 -0.025684421104138266 1106 60-61 -0.07928358301459326 1106 62-63 -0.021409322918941598 1106 64-65 -0.13039304591065104 1106 66-67 -0.06920354444697097 1106 68-69 -0.19216301794750734 1106 70-71 -0.2254877832974742 1106 72-73 -0.3036654758026529 1106 74-75 -0.3704359401623236 1106 76-77 -0.31261400206946277 1106 78-79 -0.23661751675826537 1106 80-81 -0.4016796738444661 1106 82-83 -0.5857107493604943 1106 84-85 -0.4993710054609437 1106 86-87 -0.26732980902918513 1106 88-89 -0.1895440023373176 1106 90-91 -0.3146843365036638 1106 92-93 -0.09862178916555209 1106 94-95 -0.18410357804842192 1106 96-97 0.05628991851798304 1106 98-99 0.22507934371119376 1106 100-101 0.15854005443607022 1107 1 0.04836179335590174 1107 2 0.07649603820087947 1107 3 0.04865642162899775 1107 4 0.05524565803981574 1107 5 -0.01105555484782883 1107 6 -0.009512762345188719 1107 7 0.006654580014817668 1107 8 -0.013205011765784036 1107 9 0.049353379656857044 1107 10-11 0.01727291433535072 1107 12-13 0.07021559589224324 1107 14-15 0.04227852844456237 1107 16-17 0.08038812572206666 1107 18-19 0.12633007300420473 1107 20-21 0.06503090171880643 1107 22-23 0.07041624467223073 1107 24-25 0.08764843480825135 1107 26-27 0.024065736680746852 1107 28-29 0.020934137700642452 1107 30-31 -0.03941248889046278 1107 32-33 -0.04708179411230873 1107 34-35 0.030169914210311788 1107 36-37 0.14454628460877927 1107 38-39 0.127932593533977 1107 40-41 0.20861656488108338 1107 42-43 0.2575357105445377 1107 44-45 0.14144108673898614 1107 46-47 0.17627818929953065 1107 48-49 0.22580479648320306 1107 50-51 0.2638530958870007 1107 52-53 0.3327878125592747 1107 54-55 0.2312113820302173 1107 56-57 0.26365879795532265 1107 58-59 0.2742744032554114 1107 60-61 0.23425087679851941 1107 62-63 0.28240634597531766 1107 64-65 0.3079566594289602 1107 66-67 0.19913319190568046 1107 68-69 0.1785936134742414 1107 70-71 0.2226169761063801 1107 72-73 0.2630512983136768 1107 74-75 0.3660990895002172 1107 76-77 0.2104630240667973 1107 78-79 0.28719942045290026 1107 80-81 0.3896268176158344 1107 82-83 0.3704764041969568 1107 84-85 0.47401068468744967 1107 86-87 0.4708242896887427 1107 88-89 0.696872144944642 1107 90-91 0.5430026464562303 1107 92-93 0.5099441731386385 1107 94-95 0.34377170531322676 1107 96-97 0.46880284731181376 1107 98-99 0.6644989904760017 1107 100-101 0.6648925357819877 1108 1 0.04643633630141153 1108 2 0.03822687652134249 1108 3 0.047173503472343725 1108 4 -0.0029488219067062005 1108 5 0.0035694861076578377 1108 6 0.054032766545013544 1108 7 0.06284120880479094 1108 8 -0.017047668896516655 1108 9 0.04311075274314646 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-0.3374889374111305 2316 56-57 -0.4416831237960821 2316 58-59 -0.525024860640606 2316 60-61 -0.31826589467979005 2316 62-63 -0.323889135657609 2316 64-65 -0.374455655697183 2316 66-67 -0.28565749164713594 2316 68-69 -0.3424605268280132 2316 70-71 -0.39111351100130065 2316 72-73 -0.18859371158609406 2316 74-75 -0.13267078676067 2316 76-77 -0.18683666295174106 2316 78-79 -0.17680076688378676 2316 80-81 -0.18133785326914165 2316 82-83 -0.17742582866254253 2316 84-85 -0.15577197245734808 2316 86-87 -0.22508159623613366 2316 88-89 -0.30556371646497027 2316 90-91 -0.2212665143723136 2316 92-93 -0.4169189386529162 2316 94-95 -0.35999085512708007 2316 96-97 -0.4029401590544026 2316 98-99 -0.30410822586233976 2316 100-101 -0.14697104435127883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 15.0 5 33.0 6 48.0 7 153.0 8 404.0 9 870.0 10 1401.0 11 2379.0 12 3583.0 13 5144.0 14 7405.0 15 9578.0 16 12604.0 17 17499.0 18 17290.0 19 12647.0 20 11073.0 21 11225.0 22 11633.0 23 12553.0 24 14702.0 25 16924.0 26 20185.0 27 24556.0 28 30112.0 29 37149.0 30 45900.0 31 56200.0 32 70710.0 33 92765.0 34 130157.0 35 195634.0 36 324452.0 37 537494.0 38 474064.0 39 50186.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89041048585538 18.695970935982835 15.067484586525751 23.346133991636037 2 15.793696549122336 19.57352653359795 40.39201516838161 24.240761748898105 3 19.55084128154972 23.28425230388324 29.60265264250338 27.56225377206366 4 13.24088938159273 16.728721967753675 36.2703863839011 33.7600022667525 5 14.075470461824258 36.87576868144956 34.13242261829394 14.916338238432258 6 33.02379470305985 35.61417924522455 16.97341612882946 14.388609922886141 7 29.227736904412993 32.23583257553555 19.934981392264177 18.601449127787287 8 26.275918371683858 35.27444231836885 19.094215057514724 19.355424252432567 9 25.99464211478412 17.09245960224498 19.916793840070092 36.9961044429008 10-11 25.212375228999555 26.353543438858335 26.818581880678504 21.615499451463606 12-13 26.065373673263565 23.46416450247882 27.404063685119745 23.066398139137874 14-15 22.822080294394482 25.179591435571545 25.633406796184417 26.364921473849556 16-17 22.349092842597713 28.3019252177605 26.52807560349692 22.820906336144866 18-19 22.28808741872674 26.760120031628283 28.7893062409186 22.16248630872638 20-21 22.795273289775086 25.89738163523171 29.19528515480768 22.11205992018552 22-23 22.64116068353213 25.813241730559277 29.01711043954633 22.52848714636226 24-25 22.515491787891346 26.030033436405304 29.132552855376918 22.321921920326428 26-27 22.232529984702936 26.354965833457506 28.881649346304584 22.530854835534974 28-29 22.27516477678349 26.58080011510855 28.856296935788244 22.287738172319713 30-31 22.415902154211775 26.18887183905349 28.79331290304586 22.601913103688876 32-33 22.169808292670727 26.101244084340337 28.850776509537774 22.878171113451167 34-35 22.769940890132872 26.27594995786723 28.665805856256178 22.288303295743727 36-37 22.832750943316867 26.007918438095277 28.649980464015602 22.50935015457225 38-39 22.330720446164968 26.323976606892398 28.660719128835893 22.684583818106745 40-41 22.753286730998376 26.130967114314167 28.652200076248707 22.463546078438753 42-43 22.51708452703826 26.089842175254518 28.4733188269728 22.919754470734425 44-45 22.549140058658377 26.39301465485353 28.53214821190244 22.525697074585658 46-47 22.814133378904504 26.55887933881663 27.969733541473158 22.657253740805707 48-49 22.74686130042528 26.54358318671754 28.03226846141518 22.677287051442 50-51 22.796933508888593 26.795715129421826 27.871382161189125 22.535969200500457 52-53 22.943209880915806 26.633589198924355 27.75731403996391 22.66588688019593 54-55 22.708012173169266 26.75722018224336 27.77604131156425 22.75872633302313 56-57 22.6732139805502 26.873391247679013 27.668791156831823 22.784603614938963 58-59 22.743098137101335 26.583782625519202 27.771658333104593 22.901460904274863 60-61 22.657032378257238 26.697983564273503 27.905848309985487 22.739135747483772 62-63 22.499377971238808 26.818847515736664 28.00922462011973 22.6725498929048 64-65 22.43958794689778 26.73687696403921 27.82491816226583 22.998616926797176 66-67 22.40870787138659 26.842688262206593 27.70033531998842 23.0482685464184 68-69 22.574534809770018 26.607094750072662 27.85368215231016 22.964688287847157 70-71 22.533246441154308 26.66506695331641 27.638685850240268 23.163000755289016 72-73 22.635316712252592 26.755250055562 27.50812621915423 23.101307013031168 74-75 22.587391720509416 27.08237962968702 27.22338757306071 23.106841076742853 76-77 22.669827133558652 26.771276704071035 27.46994117954362 23.088954982826692 78-79 22.578802853628886 26.763816786187682 27.588923549344813 23.06845681083862 80-81 22.66995995108773 26.870181490726235 27.36510387658949 23.094754681596537 82-83 22.73775626329145 26.896054497724727 27.302255043915036 23.063934195068793 84-85 22.66834790246943 26.965150957029316 27.35347923721009 23.01302190329116 86-87 22.7195240164455 26.850445172039993 27.210580619625997 23.21945019188851 88-89 22.598519127839833 26.976541973798184 27.049480981956066 23.37545791640592 90-91 22.625151965658638 26.93349460361424 27.180203600778135 23.26114982994899 92-93 22.614740789358635 26.700350749812117 27.211633072973452 23.4732753878558 94-95 22.446228823351802 26.676533533327017 27.341373811393797 23.535863831927387 96-97 22.679864897949606 26.467579005435336 27.281551592770647 23.571004503844406 98-99 22.4899479266741 26.590246411934455 27.12928635370725 23.790519307684203 100-101 22.562643387590768 26.2999072933647 27.363532202495378 23.773917116549153 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 504.0 1 346.5 2 278.5 3 384.5 4 321.0 5 287.5 6 576.0 7 1411.5 8 2356.5 9 2820.5 10 2427.5 11 1910.5 12 1929.0 13 2038.0 14 2211.0 15 2362.0 16 2471.5 17 2639.5 18 2910.5 19 3143.5 20 3478.5 21 4109.5 22 4943.0 23 6042.5 24 7475.0 25 9541.5 26 12279.5 27 15241.5 28 17840.0 29 20735.5 30 24293.5 31 28233.5 32 33052.5 33 36407.5 34 38397.5 35 41905.5 36 47136.0 37 51970.0 38 55269.0 39 58089.5 40 61516.0 41 66460.5 42 69854.5 43 71368.0 44 74582.0 45 78986.0 46 81991.5 47 84802.0 48 86289.0 49 86020.0 50 86230.5 51 84935.5 52 84461.5 53 86163.5 54 87449.5 55 82287.0 56 72998.0 57 64996.5 58 58994.0 59 51781.5 60 41776.5 61 34742.0 62 29048.0 63 21945.0 64 15517.0 65 11461.0 66 8413.5 67 6295.5 68 4747.0 69 3481.0 70 2786.5 71 1932.0 72 1355.5 73 1181.0 74 866.0 75 580.0 76 410.0 77 234.0 78 138.0 79 66.0 80 25.5 81 17.0 82 13.5 83 7.5 84 3.5 85 3.0 86 1.0 87 1.5 88 1.5 89 1.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015761013450873895 3 0.007083601550954559 4 0.0 5 0.0 6 0.0 7 0.0 8 5.312701163215919E-4 9 4.86997606628126E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.87771313007529E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005401246182602851 26-27 0.020431763223534558 28-29 2.2136254846733E-5 30-31 0.0 32-33 3.76316332394461E-4 34-35 0.017753276387079863 36-37 0.0037852995787913428 38-39 0.01684568993836381 40-41 0.015008380786084975 42-43 0.00834536807721834 44-45 0.002944121894615489 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.091338614748589E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.640876454019899E-5 84-85 0.0036303457948642114 86-87 1.9922629362059699E-4 88-89 6.640876454019899E-5 90-91 1.77090038773864E-4 92-93 2.43498803314063E-4 94-95 0.0 96-97 4.4272509693466E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2258738.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.943889007964344 #Duplication Level Percentage of deduplicated Percentage of total 1 75.00902729114877 40.46278642789097 2 13.906040057984583 15.002917628564527 3 4.659313121210442 7.54024409591784 4 2.1100749373894967 4.553025928841054 5 1.1311175390313672 3.0508439490234927 6 0.6712019635068653 2.1724346532805248 7 0.4627873775596155 1.7475185650553953 8 0.3162285837540971 1.3646879698541416 9 0.24593955635442938 1.1940242517586197 >10 1.3523697484895978 13.520518060529179 >50 0.09415090640354766 3.4380942300705057 >100 0.03814369655960228 3.5849380622548965 >500 0.0025400417918077863 0.8875784671578315 >1k 9.013051519317147E-4 0.7956426466464306 >5k 1.6387366398758451E-4 0.6847450631546305 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8373 0.37069372366339076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7119 0.31517599650778444 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4484 0.1985179334655015 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.4272509693466E-5 0.0 8 0.0 0.0 0.0 4.4272509693466E-5 0.0 9 0.0 0.0 0.0 4.4272509693466E-5 0.0 10-11 0.0 0.0 0.0 6.6408764540199E-5 0.0 12-13 0.0 0.0 0.0 8.8545019386932E-5 0.0 14-15 0.0 0.0 0.0 8.8545019386932E-5 0.0 16-17 0.0 0.0 0.0 8.8545019386932E-5 0.0 18-19 0.0 0.0 0.0 2.2136254846733E-4 0.0 20-21 0.0 0.0 0.0 2.6563505816079596E-4 0.0 22-23 4.4272509693466E-5 0.0 0.0 3.09907567854262E-4 0.0 24-25 4.4272509693466E-5 0.0 0.0 5.75542626015058E-4 0.0 26-27 4.4272509693466E-5 0.0 0.0 9.96131468102985E-4 0.0 28-29 4.4272509693466E-5 0.0 0.0 0.0032540294624697503 0.0 30-31 4.4272509693466E-5 0.0 0.0 0.009850633406796185 0.0 32-33 4.4272509693466E-5 0.0 0.0 0.02525746678012235 0.0 34-35 4.4272509693466E-5 0.0 0.0 0.050094344718156776 0.0 36-37 4.4272509693466E-5 0.0 0.0 0.09067009985221836 0.0 38-39 6.6408764540199E-5 0.0 0.0 0.14968535527360854 0.0 40-41 8.8545019386932E-5 0.0 0.0 0.21157832382507402 0.0 42-43 8.8545019386932E-5 0.0 0.0 0.27836340469766746 0.0 44-45 8.8545019386932E-5 0.0 0.0 0.3530511285505446 0.0 46-47 8.8545019386932E-5 0.0 0.0 0.4407771065081475 0.0 48-49 8.8545019386932E-5 0.0 0.0 0.5370255425817425 0.0 50-51 8.8545019386932E-5 0.0 0.0 0.6355982854142446 0.0 52-53 8.8545019386932E-5 0.0 0.0 0.7343923907952139 0.0 54-55 8.8545019386932E-5 0.0 0.0 0.8377465646746103 0.0 56-57 8.8545019386932E-5 0.0 0.0 0.9577914747084434 0.0 58-59 8.8545019386932E-5 0.0 0.0 1.0907860938276153 0.0 60-61 8.8545019386932E-5 0.0 0.0 1.2282301001709803 0.0 62-63 8.8545019386932E-5 0.0 0.0 1.3649436101044035 0.0 64-65 8.8545019386932E-5 0.0 0.0 1.506504959849261 0.0 66-67 8.8545019386932E-5 0.0 0.0 1.664535683200088 0.0 68-69 8.8545019386932E-5 0.0 0.0 1.840209001663761 0.0 70-71 8.8545019386932E-5 0.0 0.0 2.0248917758500546 0.0 72-73 8.8545019386932E-5 0.0 0.0 2.2057007054381694 0.0 74-75 8.8545019386932E-5 0.0 0.0 2.3940580979290207 0.0 76-77 8.8545019386932E-5 0.0 0.0 2.597999413831972 0.0 78-79 8.8545019386932E-5 0.0 0.0 2.823988439562269 0.0 80-81 8.8545019386932E-5 0.0 0.0 3.058455650898865 0.0 82-83 8.8545019386932E-5 0.0 0.0 3.304323033481528 0.0 84-85 8.8545019386932E-5 0.0 0.0 3.558757146689877 0.0 86-87 8.8545019386932E-5 0.0 0.0 3.8225770319532413 0.0 88-89 8.8545019386932E-5 0.0 0.0 4.098992446224396 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4175 0.0 56.209187 1 GTATCAA 7395 0.0 43.040115 1 ATCAACG 9530 0.0 33.296696 3 TATCAAC 9620 0.0 32.93727 2 TCAACGC 9685 0.0 32.76091 4 CAACGCA 9925 0.0 32.016567 5 AACGCAG 10220 0.0 31.09241 6 TGGTATC 1740 0.0 30.30474 2 ACGCAGA 11765 0.0 26.72669 7 CGCAGAG 11830 0.0 26.579844 8 GTGGTAT 2160 0.0 25.291868 1 TACATGG 7450 0.0 24.039305 2 GCAGAGT 13035 0.0 23.68544 9 GTACATG 7830 0.0 23.661358 1 GTACGGG 165 2.4295077E-6 23.032595 1 GAGTACT 8080 0.0 22.573008 12-13 GGACCGA 485 0.0 22.525023 6 ACATGGG 7920 0.0 22.311838 3 GTACTTT 8410 0.0 21.630785 14-15 CAGAGTA 12415 0.0 21.309734 10-11 >>END_MODULE