FastQCFastQC Report
Thu 26 May 2016
SRR938280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298012
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83370.36279183920710595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69010.30030304454458895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41130.17898078861206992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41800.055.1292461
GTATCAA75500.042.8189281
ATCAACG95350.033.369093
TCAACGC96650.032.821844
TATCAAC97150.032.799782
CAACGCA98300.032.174155
AACGCAG102100.031.0171686
TGGTATC17700.029.5568662
GTGGTAT19900.027.7535061
ACGCAGA117700.026.7357967
CGCAGAG117350.026.6922688
TAAGGTG11550.023.8827845
GCAGAGT128600.023.4331939
GTACATG75600.022.7351781
GAGTACT80450.022.63023412-13
TACATGG73550.022.3087562
AGAGTAC116500.021.23579410-11
CAGAGTA123150.021.0925710-11
GTACTTT86250.020.77639414-15
AGTACTT84950.020.28434412-13
Produced by FastQC (version 0.11.5)