FastQCFastQC Report
Thu 26 May 2016
SRR938280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298012
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82560.3592670534357523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71170.3097024732681988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43080.18746638398755097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45400.055.3543621
GTATCAA77750.040.8768841
ATCAACG97650.032.1497273
TCAACGC98650.031.8685054
TATCAAC99850.031.6364542
CAACGCA101850.030.9138645
AACGCAG105350.029.9769826
TGGTATC18400.029.1725792
ACGCAGA121550.025.8644837
CGCAGAG122400.025.6460728
GTGGTAT23150.025.0357651
GCAGAGT135150.023.1212169
TACATGG74050.023.0294512
GTACATG76650.022.0642851
GAGTACT84000.021.9367412-13
GTACTTT85650.021.26415814-15
CAGAGTA128850.021.0456310-11
ACATGGG78300.020.7449513
AGAGTAC120650.020.62596110-11
AGTACTT87900.019.50464212-13
Produced by FastQC (version 0.11.5)