##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938280_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2298012 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50071801191638 34.0 31.0 34.0 31.0 34.0 2 32.71877779576434 34.0 31.0 34.0 31.0 34.0 3 32.7736082318108 34.0 31.0 34.0 31.0 34.0 4 36.23247311154163 37.0 35.0 37.0 35.0 37.0 5 36.202528098199664 37.0 35.0 37.0 35.0 37.0 6 36.23175553478398 37.0 37.0 37.0 35.0 37.0 7 36.188872817026194 37.0 37.0 37.0 35.0 37.0 8 36.177025185247075 37.0 37.0 37.0 35.0 37.0 9 37.87012121781783 39.0 38.0 39.0 35.0 39.0 10-11 37.84571468730364 39.0 38.0 39.0 35.0 39.0 12-13 37.759922271946365 39.0 38.0 39.0 35.0 39.0 14-15 39.042301563264246 40.0 38.0 41.0 36.0 41.0 16-17 38.92190140869586 40.0 38.0 41.0 35.0 41.0 18-19 39.172013462070694 40.0 39.0 41.0 36.0 41.0 20-21 39.15310168093117 40.0 39.0 41.0 36.0 41.0 22-23 39.05689330604018 40.0 39.0 41.0 35.5 41.0 24-25 38.957493694549896 40.0 38.0 41.0 35.0 41.0 26-27 38.78539276557303 40.0 38.0 41.0 35.0 41.0 28-29 38.668721921382485 40.0 38.0 41.0 34.5 41.0 30-31 38.442267707914496 40.0 38.0 41.0 34.0 41.0 32-33 38.237460030669986 40.0 38.0 41.0 34.0 41.0 34-35 38.09285808777326 40.0 38.0 41.0 34.0 41.0 36-37 38.096317599734036 40.0 38.0 41.0 33.5 41.0 38-39 37.953869475007096 40.0 38.0 41.0 33.0 41.0 40-41 37.782452397985736 40.0 37.0 41.0 33.0 41.0 42-43 37.555607629551105 40.0 37.0 41.0 33.0 41.0 44-45 37.287626217791725 40.0 37.0 41.0 32.0 41.0 46-47 36.9020849325417 40.0 36.0 41.0 31.0 41.0 48-49 36.51932779289229 39.0 36.0 41.0 30.0 41.0 50-51 36.14076667136638 39.0 35.0 40.5 29.5 41.0 52-53 35.625819186322786 38.5 35.0 40.0 28.0 41.0 54-55 35.31064241614056 38.0 34.5 40.0 27.5 41.0 56-57 34.955703451505045 38.0 34.0 40.0 26.5 41.0 58-59 34.51124645998367 38.0 34.0 40.0 26.0 41.0 60-61 34.02967847861543 37.0 33.0 40.0 25.0 41.0 62-63 33.5514470768647 36.5 33.0 39.0 23.0 41.0 64-65 33.05217335679709 36.0 32.0 39.0 22.0 40.0 66-67 32.48121267425931 35.5 31.0 39.0 20.5 40.0 68-69 31.884489071423474 35.0 31.0 38.0 18.5 40.0 70-71 31.249902524442867 35.0 30.0 37.5 15.0 39.5 72-73 30.647558628936665 34.0 29.5 37.0 9.5 39.0 74-75 30.02324683247955 34.0 29.0 36.0 7.5 39.0 76-77 28.674159447383218 32.5 27.0 35.0 7.0 37.0 78-79 29.355316682419414 34.0 29.0 35.0 2.0 37.0 80-81 29.01348818021838 34.0 29.0 35.0 2.0 37.0 82-83 28.421264771463335 33.5 27.5 35.0 2.0 36.5 84-85 27.802927922047402 33.0 26.0 35.0 2.0 36.0 86-87 27.126931887213818 32.5 25.5 35.0 2.0 35.5 88-89 26.355115421503456 32.0 24.5 34.5 2.0 35.0 90-91 25.781433473802572 32.0 23.5 34.0 2.0 35.0 92-93 24.913802016699652 31.0 19.0 34.0 2.0 35.0 94-95 24.037789402318175 31.0 11.5 34.0 2.0 35.0 96-97 22.945559248602706 30.5 2.0 34.0 2.0 35.0 98-99 21.445796192535113 30.0 2.0 34.0 2.0 35.0 100-101 17.094832620543322 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05734021869987771 1101 2 0.07996912595081795 1101 3 0.03252297220958411 1101 4 0.02232898480752965 1101 5 0.08590641426322776 1101 6 0.001929929163821953 1101 7 0.019118388460427127 1101 8 0.003595302228021069 1101 9 -0.029075543027936135 1101 10-11 0.006190553851766367 1101 12-13 0.08264987724500372 1101 14-15 0.1171154002953756 1101 16-17 0.15812884059812404 1101 18-19 0.16386775029647538 1101 20-21 0.12206366797613555 1101 22-23 0.10772973969129396 1101 24-25 0.1187244700872796 1101 26-27 0.13793505594279765 1101 28-29 0.05048210423260002 1101 30-31 0.13503810833616825 1101 32-33 0.11399863659028142 1101 34-35 0.13863500165041387 1101 36-37 0.32238920669461635 1101 38-39 0.17742771211594288 1101 40-41 0.13867927835418925 1101 42-43 0.11085779953074848 1101 44-45 0.17892869849247006 1101 46-47 0.3187926389312352 1101 48-49 0.3698272157129239 1101 50-51 0.17362197341303442 1101 52-53 -0.09662247139236513 1101 54-55 -0.1332066923202504 1101 56-57 -0.07649569373957377 1101 58-59 0.02823172729534207 1101 60-61 -0.16286450080398396 1101 62-63 -0.09091363672205688 1101 64-65 0.05885306247281363 1101 66-67 -0.21519260260244977 1101 68-69 -0.021863323845465743 1101 70-71 -0.2217557531252865 1101 72-73 -0.3613968056476651 1101 74-75 -0.4178352081093415 1101 76-77 -0.5971885751462374 1101 78-79 -0.5683492381462898 1101 80-81 -0.4334591589332639 1101 82-83 -0.4043882829546561 1101 84-85 -0.6301872145496965 1101 86-87 -0.6118869004283134 1101 88-89 -0.6080404814783371 1101 90-91 -0.6768661498949946 1101 92-93 -0.8141608172788537 1101 94-95 -1.0561338979163999 1101 96-97 -1.0824736438744402 1101 98-99 -1.1492292753290627 1101 100-101 -1.5817567451830534 1102 1 0.09575912889103222 1102 2 0.17121597589277116 1102 3 0.14875522637697003 1102 4 0.13496634127118057 1102 5 0.0693467832767709 1102 6 0.09113584262110663 1102 7 0.11558982905776105 1102 8 0.09748602111317695 1102 9 0.10344487644945843 1102 10-11 0.11993523409081064 1102 12-13 0.21065246066339682 1102 14-15 0.16225578265747487 1102 16-17 0.11964869332349792 1102 18-19 0.1895920445418966 1102 20-21 0.15020373707403678 1102 22-23 0.16815557225719857 1102 24-25 0.16966967851583803 1102 26-27 0.17361975844743682 1102 28-29 0.11844155876636364 1102 30-31 0.028104789564906696 1102 32-33 0.05792367071450144 1102 34-35 0.06634681566585243 1102 36-37 0.00820982181605956 1102 38-39 -0.06263637445824344 1102 40-41 -0.13739704484996906 1102 42-43 -0.15445720177937972 1102 44-45 -0.1451932291475444 1102 46-47 -0.20847763885814885 1102 48-49 -0.3093767606300162 1102 50-51 -0.31028222437217323 1102 52-53 -0.2736618011058596 1102 54-55 -0.2209670735227789 1102 56-57 -0.2549723533094834 1102 58-59 -0.28959806192774096 1102 60-61 -0.36388371059616276 1102 62-63 -0.45249838951975363 1102 64-65 -0.28931009469098257 1102 66-67 -0.1821992980067293 1102 68-69 -0.38005666583513786 1102 70-71 -0.4605790303182573 1102 72-73 -0.41798357870730385 1102 74-75 -0.4769777586012829 1102 76-77 -0.5494490437661135 1102 78-79 -0.6706258418949425 1102 80-81 -0.64137197889983 1102 82-83 -0.7710142522421783 1102 84-85 -0.8184194471436435 1102 86-87 -0.7553513417955493 1102 88-89 -0.8801187534824493 1102 90-91 -1.0736398657252586 1102 92-93 -1.0947375604907528 1102 94-95 -1.0695392011152443 1102 96-97 -1.251611367311142 1102 98-99 -1.2388493537215162 1102 100-101 -1.3735609986546926 1103 1 0.1458631733351865 1103 2 0.12532945888587932 1103 3 0.14451954788402332 1103 4 0.0539493203348016 1103 5 0.030040023953283423 1103 6 0.022034989737576893 1103 7 0.0757996898348452 1103 8 0.052788614337217155 1103 9 0.055699604907502476 1103 10-11 0.11072800305597497 1103 12-13 0.19656011068511248 1103 14-15 0.13025352674055313 1103 16-17 0.13139361480938305 1103 18-19 0.20197949179038943 1103 20-21 0.1439362207650916 1103 22-23 0.1867169714442909 1103 24-25 0.16316555023109203 1103 26-27 0.18889041445683574 1103 28-29 0.21017526549284327 1103 30-31 0.17584519921694408 1103 32-33 0.1806049273725847 1103 34-35 0.14994377147451843 1103 36-37 0.05706997873625852 1103 38-39 0.14761799962205657 1103 40-41 0.1369753932041533 1103 42-43 0.13213327367023453 1103 44-45 0.11074508319322973 1103 46-47 0.030430553534394278 1103 48-49 0.04104708812847235 1103 50-51 0.00395274104357668 1103 52-53 0.10231624908181658 1103 54-55 0.017038968185751457 1103 56-57 0.1334884371689924 1103 58-59 0.12169697267810875 1103 60-61 0.0865976165053084 1103 62-63 -0.13334913382107771 1103 64-65 -0.08734167752786703 1103 66-67 -0.08742561358011613 1103 68-69 0.10129204403536107 1103 70-71 -0.323183317856909 1103 72-73 -0.40074169644282875 1103 74-75 -0.4112978395031348 1103 76-77 -0.38545252704012967 1103 78-79 -0.2563620292656985 1103 80-81 -0.27383298114062526 1103 82-83 -0.2975410477381253 1103 84-85 -0.2678039318805503 1103 86-87 -0.7067326587410712 1103 88-89 -0.9133168182341755 1103 90-91 -0.9803876287156932 1103 92-93 -0.9211057503335098 1103 94-95 -0.8602250085022902 1103 96-97 -1.0221729480087909 1103 98-99 -1.1847922686514103 1103 100-101 -1.464647533812638 1104 1 0.08750411142921877 1104 2 0.08703828005312886 1104 3 0.11331573319431953 1104 4 0.11467541092358857 1104 5 -0.029542305461234264 1104 6 0.0346010264073513 1104 7 0.0680219927741561 1104 8 0.04952098471566302 1104 9 0.11259546775301743 1104 10-11 0.08982671602196035 1104 12-13 0.11379371401712035 1104 14-15 0.18328990189744587 1104 16-17 0.06344425267619158 1104 18-19 0.08908917420900053 1104 20-21 0.1376594580047339 1104 22-23 0.14712644924507146 1104 24-25 0.17132655257592688 1104 26-27 0.1421728758437979 1104 28-29 0.0031267464496664843 1104 30-31 5.206156162316233E-4 1104 32-33 0.15585847993354207 1104 34-35 0.007312636471148437 1104 36-37 -0.07025094030853296 1104 38-39 0.0706711033959877 1104 40-41 0.13095410003564467 1104 42-43 0.0895379568758301 1104 44-45 -0.04527429294437724 1104 46-47 0.015696859395667673 1104 48-49 0.0072242056404476784 1104 50-51 0.08383521695961349 1104 52-53 -0.07837299415510302 1104 54-55 -0.17454587153029166 1104 56-57 -0.17547549843490629 1104 58-59 -0.055109475810262154 1104 60-61 -0.19328001638529457 1104 62-63 -0.15483874754944082 1104 64-65 -0.25475306025198563 1104 66-67 -0.48123602527763865 1104 68-69 -0.4918585054462312 1104 70-71 -0.5710587431350831 1104 72-73 -0.7988535219001918 1104 74-75 -0.6882619890073265 1104 76-77 -0.6865324208164374 1104 78-79 -0.7558777267218986 1104 80-81 -0.7856873957601032 1104 82-83 -0.6735890582636337 1104 84-85 -0.8238372317162934 1104 86-87 -0.8495083782330362 1104 88-89 -1.0570164457248055 1104 90-91 -1.1994629942632145 1104 92-93 -1.176910519906393 1104 94-95 -1.218327490956412 1104 96-97 -1.3552075272104176 1104 98-99 -1.6479236276447047 1104 100-101 -1.86268358610117 1105 1 0.05700601343222189 1105 2 0.0760247045602469 1105 3 0.08745125899429951 1105 4 0.020890997762244012 1105 5 0.008864633556989077 1105 6 0.055086233959869446 1105 7 0.017789980360674917 1105 8 0.025726463388366483 1105 9 -0.05270694721797753 1105 10-11 0.025402939932256174 1105 12-13 0.03442483532212037 1105 14-15 0.0063927113752768605 1105 16-17 -0.054554848733573635 1105 18-19 0.008839727742142145 1105 20-21 0.033381490455298035 1105 22-23 0.08927609842078965 1105 24-25 0.005278397335693796 1105 26-27 0.020300795969809826 1105 28-29 -0.01589318619893021 1105 30-31 -0.00797955700972608 1105 32-33 0.09291215608723036 1105 34-35 0.016173063508034602 1105 36-37 0.023086515784846995 1105 38-39 -0.00635563453442245 1105 40-41 -0.005330591743231139 1105 42-43 0.01187877664263226 1105 44-45 0.10241861754496995 1105 46-47 -0.13848345563307873 1105 48-49 -0.10430268854957347 1105 50-51 -0.08203222656285902 1105 52-53 -0.0700966897379871 1105 54-55 -0.06776755522184885 1105 56-57 -0.05774644170526244 1105 58-59 -0.027147284403525873 1105 60-61 -0.12801765999022052 1105 62-63 -0.18555671958792885 1105 64-65 -0.16284249608850843 1105 66-67 -0.31772358179023996 1105 68-69 -0.35179669521944845 1105 70-71 -0.4471745771055353 1105 72-73 -0.4094414945076075 1105 74-75 -0.5284446374091551 1105 76-77 -0.6017063405013339 1105 78-79 -0.4580125994268123 1105 80-81 -0.6130675687913261 1105 82-83 -0.5198970058383345 1105 84-85 -0.47144402406089725 1105 86-87 -0.6700721637217022 1105 88-89 -0.7604386093399285 1105 90-91 -0.7610701030238083 1105 92-93 -0.635880289709867 1105 94-95 -0.5398898182817398 1105 96-97 -0.8075876028229274 1105 98-99 -1.007241357617172 1105 100-101 -1.4832983800294137 1106 1 0.2361693955216353 1106 2 0.28886797526640606 1106 3 0.1783707259712557 1106 4 0.1333601042314001 1106 5 0.19250222051964272 1106 6 0.16349860069855993 1106 7 0.19750317292535158 1106 8 0.18726304599206145 1106 9 0.2108805439275443 1106 10-11 0.205816718946636 1106 12-13 0.13438941740176347 1106 14-15 0.3027372267049131 1106 16-17 0.3987320230979634 1106 18-19 0.358732363701435 1106 20-21 0.34140525932172494 1106 22-23 0.41587362427365093 1106 24-25 0.40361608666612625 1106 26-27 0.42283301073341306 1106 28-29 0.421598514460797 1106 30-31 0.4281007561779546 1106 32-33 0.4835556075469185 1106 34-35 0.4380381334445502 1106 36-37 0.4764224619762629 1106 38-39 0.4998068615518392 1106 40-41 0.3475764534251695 1106 42-43 0.38707790529517183 1106 44-45 0.3805224597786747 1106 46-47 0.48112452683338347 1106 48-49 0.4325377220762405 1106 50-51 0.352425079854342 1106 52-53 0.19210328494764184 1106 54-55 0.11718615722016779 1106 56-57 0.26522779758664683 1106 58-59 0.2650820904868283 1106 60-61 0.16344984101458238 1106 62-63 0.31218574883016714 1106 64-65 0.33974167976202096 1106 66-67 0.22961496689664074 1106 68-69 0.09053804520827669 1106 70-71 -0.24379610720410483 1106 72-73 -0.23115923033655505 1106 74-75 -0.0102887896773467 1106 76-77 -0.10168069304176086 1106 78-79 -0.20707152640816062 1106 80-81 -0.20512962454068884 1106 82-83 -0.49390846836528723 1106 84-85 -0.41851055423718364 1106 86-87 -0.4648516288777991 1106 88-89 -0.6539743392488653 1106 90-91 -0.8346785340432419 1106 92-93 -0.8640268777095201 1106 94-95 -1.0558513093428772 1106 96-97 -1.1157742497118122 1106 98-99 -0.9312718059258849 1106 100-101 -1.679965542620982 1107 1 0.022167920180613976 1107 2 0.03496178576251907 1107 3 0.005059318976833538 1107 4 -0.013032849996349682 1107 5 0.01294343714616275 1107 6 -0.007482419569043941 1107 7 -0.002321986937218412 1107 8 -0.005896180798828254 1107 9 -0.06982835335693238 1107 10-11 -0.006676199824021012 1107 12-13 0.0354182843643116 1107 14-15 0.04306538819361805 1107 16-17 0.012659935164379021 1107 18-19 -0.03469910833840828 1107 20-21 0.011077627651609134 1107 22-23 -0.022967784717771167 1107 24-25 0.0488497570455948 1107 26-27 -0.06129749581392474 1107 28-29 -0.05970072300797824 1107 30-31 -0.14431260829241666 1107 32-33 -0.04964751267707612 1107 34-35 0.09314986403586545 1107 36-37 -0.0353026273796857 1107 38-39 0.0238486031063303 1107 40-41 -0.07268523620709999 1107 42-43 -0.11524229261245011 1107 44-45 -0.2649298626607788 1107 46-47 -0.07889434285976904 1107 48-49 -0.11809602247783602 1107 50-51 -0.4113887174370632 1107 52-53 -0.4768236693697716 1107 54-55 -0.2334742820402056 1107 56-57 -0.39293935610558606 1107 58-59 -0.3556878174469915 1107 60-61 -0.46208497010509575 1107 62-63 -0.5405301828150826 1107 64-65 -0.5325903779311503 1107 66-67 -0.5360352631669016 1107 68-69 -0.5002166202520328 1107 70-71 -0.4910322881086273 1107 72-73 -0.7546041108624593 1107 74-75 -0.7839635299643426 1107 76-77 -0.8925227785707861 1107 78-79 -0.7911671871938424 1107 80-81 -0.4457000761499117 1107 82-83 -0.5449120670562273 1107 84-85 -0.8549186694278994 1107 86-87 -0.9457940538982754 1107 88-89 -1.2885912600246812 1107 90-91 -1.2443094135013588 1107 92-93 -1.384135123724672 1107 94-95 -1.5129164403507147 1107 96-97 -1.6973608118819499 1107 98-99 -1.7826817347264132 1107 100-101 -2.2334578622890717 1108 1 -0.008855040900087374 1108 2 -0.03237235303909358 1108 3 -0.0543240103160727 1108 4 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2316 54-55 0.1263516081306335 2316 56-57 0.29073745030491693 2316 58-59 0.2996865644696527 2316 60-61 0.5033476788983151 2316 62-63 0.5239602511420358 2316 64-65 0.6274011753968765 2316 66-67 0.5573868910334525 2316 68-69 0.5879403794601785 2316 70-71 0.8795514292492896 2316 72-73 1.0875178725357166 2316 74-75 1.1875060601478147 2316 76-77 0.9936106638965363 2316 78-79 1.1175008361727947 2316 80-81 1.0704882883725695 2316 82-83 1.3954571384707357 2316 84-85 1.3149094258475706 2316 86-87 1.3800956497580863 2316 88-89 1.4530606436533589 2316 90-91 1.6270787933000754 2316 92-93 1.927423268567182 2316 94-95 2.382763423306546 2316 96-97 2.7750408086830802 2316 98-99 3.075863139152812 2316 100-101 3.535521454576987 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 15.0 5 35.0 6 79.0 7 172.0 8 516.0 9 962.0 10 1677.0 11 2765.0 12 4331.0 13 6481.0 14 9100.0 15 12499.0 16 16212.0 17 22420.0 18 23081.0 19 18630.0 20 18293.0 21 19233.0 22 20850.0 23 22816.0 24 25827.0 25 29406.0 26 33699.0 27 39875.0 28 47929.0 29 58022.0 30 69657.0 31 84696.0 32 105514.0 33 135061.0 34 180914.0 35 252231.0 36 356777.0 37 437979.0 38 229800.0 39 10449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7932121129829 18.67978878918478 15.160246827507539 23.366752270324774 2 15.8388737141355 19.56215758879842 40.3158669890794 24.283101707986678 3 19.560895703481656 23.292746932519343 29.595171758897802 27.551185605101196 4 13.270824204061688 16.691216882773844 36.17989644087866 33.858062472285816 5 14.059330110226764 36.904338138982325 34.14066205684097 14.89566969394993 6 32.9864045459174 35.60501554847879 16.932741623708537 14.475838281895271 7 29.192884980583216 32.20109381500183 19.96843358520321 18.637587619211736 8 26.323317719837842 35.262174435990765 19.06904750714966 19.345460337021738 9 25.97675730152845 17.0261077835973 19.89376034589898 37.10337456897527 10-11 25.234267706173856 26.33267363268773 26.80538656891261 21.6276720922258 12-13 26.003430822836123 23.47691962240061 27.396800074499854 23.12284948026341 14-15 22.810411782009844 25.17306262978609 25.628934922881168 26.387590665322897 16-17 22.337706645871346 28.28826351104657 26.507156586753517 22.866873256328567 18-19 22.304561507946868 26.772901969180317 28.784401473969673 22.138135048903138 20-21 22.81241351220098 25.88637483181115 29.18074405181522 22.12046760417265 22-23 22.645747715851787 25.742402563607154 29.06586214519332 22.545987575347734 24-25 22.49986400892972 26.00556149081027 29.109639264647324 22.384935235612687 26-27 22.26092898404543 26.305096474935453 28.90215625533841 22.53181828568071 28-29 22.313275243241833 26.604340763773372 28.8346695359937 22.2477144569911 30-31 22.455101741969077 26.11408756212887 28.827859494491925 22.60295120141013 32-33 22.162895581050055 26.114310978358684 28.85189459411004 22.870898846481218 34-35 22.770583418721994 26.300767268547038 28.646788606537527 22.28186070619344 36-37 22.77789382071366 25.96936466492602 28.700587467362926 22.552154046997387 38-39 22.36911589134443 26.29631910236847 28.65526074557047 22.67930426071663 40-41 22.771140410827012 26.128227271451443 28.601427039333988 22.499205278387553 42-43 22.536386227640673 26.090553299559758 28.430790828610103 22.942269644189466 44-45 22.56735386934446 26.38360896287748 28.474960095943796 22.574077071834264 46-47 22.831190611711342 26.57383860484628 27.957752178839794 22.637218604602587 48-49 22.796997389033944 26.541209747606615 27.98089643167972 22.68089643167972 50-51 22.82356673429263 26.79319771586129 27.83963769194415 22.543597857901922 52-53 22.95702072816771 26.639811285570268 27.687567168371835 22.715600817890184 54-55 22.703699400180778 26.73584410531717 27.745805987225502 22.814650507276557 56-57 22.667629379811665 26.8288612000054 27.685249380568948 22.818260039613993 58-59 22.771852532781082 26.581404944648867 27.731778783502513 22.91496373906753 60-61 22.70989351762601 26.694246453017247 27.812531142036406 22.783328887320334 62-63 22.477974632417265 26.818501918106808 27.957614496656912 22.745908952819015 64-65 22.451636350183403 26.695690115741975 27.794569691641268 23.058103842433354 66-67 22.452823660512706 26.819231369319922 27.665695202950907 23.06224976721646 68-69 22.58255970731527 26.62754192053679 27.740910461063333 23.048987911084605 70-71 22.616359586396957 26.6522229988571 27.478802365186873 23.252615049559072 72-73 22.610101847050785 26.74660609559179 27.43270885912895 23.210583198228473 74-75 22.64177878053536 27.05423856577811 27.10912039358235 23.194862260104177 76-77 22.669540720784045 26.675995373367705 27.468616264810503 23.18584764103775 78-79 22.594246431169015 26.699843871125562 27.5741133364179 23.131796361287527 80-81 22.705017880434145 26.82030305127204 27.33839390746565 23.136285160828162 82-83 22.718362922866973 26.858938781551107 27.226876307261026 23.195821988320894 84-85 22.72467708035462 26.90107692836683 27.25763018306868 23.116615808209865 86-87 22.769158043123266 26.75704492730629 27.2001217057346 23.273675323835846 88-89 22.70176421341178 26.866371611891765 26.97120114801073 23.460663026685722 90-91 22.71297213184326 26.831496131538568 27.07759774530181 23.377933991316365 92-93 22.73908793115305 26.619868013172564 27.076733774334038 23.564310281340344 94-95 22.51771510169033 26.60765513484977 27.18871786903914 23.68591189442076 96-97 22.722117333904343 26.403305592331748 27.135695888290893 23.738881185473012 98-99 22.580888737049857 26.470054466207234 26.91548924928898 24.033567547453927 100-101 23.046975981059898 25.79044029646135 26.91150550316176 24.25107821931699 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 494.0 1 342.0 2 264.0 3 371.0 4 323.5 5 292.0 6 584.0 7 1311.5 8 2150.0 9 2608.0 10 2293.5 11 1848.0 12 1846.5 13 1997.5 14 2148.5 15 2287.5 16 2476.5 17 2680.5 18 2952.5 19 3204.0 20 3540.5 21 4187.5 22 5067.5 23 6210.0 24 7755.0 25 9820.0 26 12688.0 27 15830.5 28 18367.5 29 21292.0 30 24858.5 31 28772.5 32 33780.5 33 37037.0 34 39032.0 35 42752.5 36 47750.5 37 52453.0 38 55996.5 39 58801.5 40 61970.0 41 66867.5 42 70617.5 43 72432.5 44 75536.0 45 80047.0 46 83006.0 47 85715.0 48 87373.0 49 87237.0 50 87305.0 51 85976.5 52 85447.5 53 87048.0 54 87995.0 55 83006.5 56 74496.0 57 66846.0 58 60521.5 59 53146.0 60 43419.5 61 36198.0 62 30294.0 63 23111.0 64 16634.0 65 12394.0 66 9119.5 67 6757.0 68 5067.0 69 3727.5 70 2959.5 71 2105.0 72 1503.5 73 1213.5 74 918.0 75 678.5 76 475.0 77 268.5 78 153.0 79 87.5 80 47.0 81 26.5 82 16.5 83 11.0 84 4.0 85 5.0 86 4.0 87 1.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009268881102448551 2 0.014621333570059687 3 0.008616142996642314 4 4.351587372041573E-5 5 9.573492218491462E-4 6 0.001653603201375798 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.138333481287305E-4 14-15 0.0 16-17 0.0025674365495045284 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002915563539267854 26-27 0.017167012182704007 28-29 0.0062880437526000735 30-31 0.0028938056024076465 32-33 0.0 34-35 0.008072194575137118 36-37 5.221904846449888E-4 38-39 0.002328099244042242 40-41 0.00163184526451559 42-43 0.0026762262338055677 44-45 0.0 46-47 0.0 48-49 5.221904846449888E-4 50-51 0.003394238150192427 52-53 0.006462107247481737 54-55 0.0066579286792236075 56-57 0.014751881191220933 58-59 0.021670905112767036 60-61 0.003176658781590348 62-63 0.018885889194660426 64-65 0.01934280586872479 66-67 0.012032139083694951 68-69 0.028698718718614173 70-71 0.015230555802145506 72-73 0.00459092467750386 74-75 0.015143524054704675 76-77 0.01934280586872479 78-79 0.02397724641994907 80-81 0.010835452556383518 82-83 0.032506357669150554 84-85 0.01997378603767082 86-87 0.0290468457083775 88-89 0.020561250332896436 90-91 0.02554381787388403 92-93 0.022193095597412026 94-95 0.014164416895995322 96-97 0.014360238327737192 98-99 0.011183579546146844 100-101 0.010530841440340607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2298012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.59117090244025 #Duplication Level Percentage of deduplicated Percentage of total 1 74.76024544533331 40.06489090369237 2 14.031314500684495 15.039091467841414 3 4.660937697793214 7.493553261841875 4 2.098020150433893 4.497414257546646 5 1.1659852629505938 3.1243257748256 6 0.7177180432236429 2.3078010188497937 7 0.45546141579453464 1.7086097401318616 8 0.3243832423074748 1.3907262221110055 9 0.25782294742952533 1.2435330274439902 >10 1.3903457670436674 13.66725606052216 >50 0.09597842396233837 3.489224398190849 >100 0.038125478839255225 3.597873847454968 >500 0.002522452229291044 0.8809015435918203 >1k 9.764331210157765E-4 0.8244307006160795 >5k 1.627388535026294E-4 0.6703677753396271 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8256 0.3592670534357523 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7117 0.3097024732681988 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4308 0.18746638398755097 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.351587372041573E-5 0.0 3 0.0 0.0 0.0 8.703174744083146E-5 0.0 4 0.0 0.0 0.0 8.703174744083146E-5 0.0 5 0.0 0.0 0.0 8.703174744083146E-5 0.0 6 0.0 0.0 0.0 8.703174744083146E-5 0.0 7 0.0 0.0 0.0 8.703174744083146E-5 0.0 8 0.0 0.0 0.0 8.703174744083146E-5 0.0 9 0.0 0.0 0.0 1.305476211612472E-4 0.0 10-11 0.0 0.0 0.0 1.7406349488166293E-4 0.0 12-13 0.0 0.0 0.0 1.7406349488166293E-4 0.0 14-15 0.0 0.0 0.0 1.7406349488166293E-4 0.0 16-17 0.0 0.0 0.0 2.1757936860207865E-4 0.0 18-19 0.0 0.0 0.0 2.3933730546228654E-4 0.0 20-21 0.0 0.0 0.0 3.4812698976332586E-4 0.0 22-23 4.351587372041573E-5 0.0 0.0 5.874642952256124E-4 0.0 24-25 4.351587372041573E-5 0.0 0.0 9.79107158709354E-4 0.0 26-27 4.351587372041573E-5 0.0 0.0 0.0015012976433543429 0.0 28-29 4.351587372041573E-5 0.0 0.0 0.0033724802133322193 0.0 30-31 4.351587372041573E-5 0.0 0.0 0.011053031924985596 0.0 32-33 4.351587372041573E-5 0.0 0.0 0.02621831391655048 0.0 34-35 4.351587372041573E-5 0.0 0.0 0.05134873099009056 0.0 36-37 4.351587372041573E-5 0.0 0.0 0.09231892609786198 0.0 38-39 4.351587372041573E-5 0.0 0.0 0.15480772076037896 0.0 40-41 4.351587372041573E-5 0.0 0.0 0.2191241821191534 0.0 42-43 4.351587372041573E-5 0.0 0.0 0.28187407202399295 0.0 44-45 4.351587372041573E-5 0.0 0.0 0.3577004819818173 0.0 46-47 4.351587372041573E-5 0.0 0.0 0.4487574477417873 0.0 48-49 4.351587372041573E-5 0.0 0.0 0.5436438103891538 0.0 50-51 4.351587372041573E-5 0.0 0.0 0.6451881017157439 0.0 52-53 4.351587372041573E-5 0.0 0.0 0.7443607779245713 0.0 54-55 4.351587372041573E-5 0.0 0.0 0.8510181844133102 0.0 56-57 4.351587372041573E-5 0.0 0.0 0.9743856864106888 0.0 58-59 4.351587372041573E-5 0.0 0.0 1.1071743750685377 0.0 60-61 4.351587372041573E-5 0.0 0.0 1.2441188296666859 0.0 62-63 4.351587372041573E-5 0.0 0.0 1.3821511811078446 0.0 64-65 4.351587372041573E-5 0.0 0.0 1.5257753223220767 0.0 66-67 4.351587372041573E-5 0.0 0.0 1.6852827574442606 0.0 68-69 4.351587372041573E-5 0.0 0.0 1.8596943793156868 0.0 70-71 4.351587372041573E-5 0.0 0.0 2.04426695770083 0.0 72-73 4.351587372041573E-5 0.0 0.0 2.23162455200408 0.0 74-75 4.351587372041573E-5 0.0 0.0 2.4171327216742124 0.0 76-77 4.351587372041573E-5 0.0 0.0 2.621178653549242 0.0 78-79 4.351587372041573E-5 0.0 0.0 2.8519433318886063 0.0 80-81 4.351587372041573E-5 0.0 0.0 3.0903015301921837 0.0 82-83 4.351587372041573E-5 0.0 0.0 3.338146188966811 0.0 84-85 4.351587372041573E-5 0.0 0.0 3.5897549708182552 0.0 86-87 4.351587372041573E-5 0.0 0.0 3.8509154869513305 0.0 88-89 4.351587372041573E-5 0.0 0.0 4.127458864444572 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4540 0.0 55.354362 1 GTATCAA 7775 0.0 40.876884 1 ATCAACG 9765 0.0 32.149727 3 TCAACGC 9865 0.0 31.868505 4 TATCAAC 9985 0.0 31.636454 2 CAACGCA 10185 0.0 30.913864 5 AACGCAG 10535 0.0 29.976982 6 TGGTATC 1840 0.0 29.172579 2 ACGCAGA 12155 0.0 25.864483 7 CGCAGAG 12240 0.0 25.646072 8 GTGGTAT 2315 0.0 25.035765 1 GCAGAGT 13515 0.0 23.121216 9 TACATGG 7405 0.0 23.029451 2 GTACATG 7665 0.0 22.064285 1 GAGTACT 8400 0.0 21.93674 12-13 GTACTTT 8565 0.0 21.264158 14-15 CAGAGTA 12885 0.0 21.04563 10-11 ACATGGG 7830 0.0 20.744951 3 AGAGTAC 12065 0.0 20.625961 10-11 AGTACTT 8790 0.0 19.504642 12-13 >>END_MODULE