FastQCFastQC Report
Thu 26 May 2016
SRR938279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049265
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128260.6258829385169804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101870.49710505961893653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65290.3186020353638988No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38110.18596911575613698No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26310.12838749502870542No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21440.10462287698272307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104900.030.1142351
GTATCAA148250.029.0979941
GTACATG106200.025.072581
GTATAGG4400.024.9439071
GAGTACT90650.024.12920812-13
ATCAACG175850.024.0526073
TACATGG109000.024.0236842
TATCAAC178250.023.7554152
CAACGCA178350.023.715455
TCAACGC179650.023.5173854
AACGCAG182450.023.1779926
ACATGGG112700.022.5602723
GTACTTT98600.022.11197914-15
AGTACTT96050.021.93196112-13
ACGCAGA200650.020.9042477
CGCAGAG202500.020.7132728
CGACGAG2300.020.66924394-95
TAGCGCG1150.003713299320.6626824
CGTACAC3001.6552804E-1020.5938033
CATGGGG54950.020.237774
Produced by FastQC (version 0.11.5)