FastQCFastQC Report
Thu 26 May 2016
SRR938279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049265
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118560.578548894359685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102250.4989593829982945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67140.327629662342352No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38060.18572512583780038No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27280.13312089944443495No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21910.10691638221508688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99450.030.8962231
GTATCAA140050.028.901751
GTACATG108750.025.4986361
TACATGG109400.024.7819352
TATCAAC169700.023.7118932
ATCAACG168900.023.7086623
CAACGCA171050.023.437895
TCAACGC171750.023.3429344
GAGTACT89450.023.2554412-13
ACATGGG113150.023.243163
AACGCAG174250.023.0311036
GTACTTT92600.022.23355514-15
AGTACTT92900.021.21598612-13
ACGCAGA191800.020.8741977
CGCAGAG192450.020.729668
AGAGTAC156850.020.21145210-11
CATGGGA56400.019.9002044
GCAGAGT200650.019.7878199
ATTCGCG2001.8189894E-1218.99910780-81
CATGGGG50900.018.8737494
Produced by FastQC (version 0.11.5)