##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938279_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2049265 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.520418784295835 34.0 31.0 34.0 31.0 34.0 2 32.74011169858461 34.0 31.0 34.0 31.0 34.0 3 32.800005367778205 34.0 31.0 34.0 31.0 34.0 4 36.24598331596938 37.0 35.0 37.0 35.0 37.0 5 36.17843665899725 37.0 37.0 37.0 35.0 37.0 6 36.23757835126253 37.0 37.0 37.0 35.0 37.0 7 36.21283679758352 37.0 37.0 37.0 35.0 37.0 8 36.21718518590812 37.0 37.0 37.0 35.0 37.0 9 37.907097422734495 39.0 38.0 39.0 35.0 39.0 10-11 37.88335378782149 39.0 38.0 39.0 35.0 39.0 12-13 37.803700595091414 39.0 38.0 39.0 35.0 39.0 14-15 39.03987698028317 40.0 38.0 41.0 36.0 41.0 16-17 38.9578939278229 40.0 38.0 41.0 36.0 41.0 18-19 39.2233857017028 40.5 39.0 41.0 36.0 41.0 20-21 39.210985646073105 41.0 39.0 41.0 36.0 41.0 22-23 39.100969127955636 40.0 39.0 41.0 35.5 41.0 24-25 38.99610714085294 40.0 38.5 41.0 35.0 41.0 26-27 38.838689725340544 40.0 38.0 41.0 35.0 41.0 28-29 38.7184356342396 40.0 38.0 41.0 35.0 41.0 30-31 38.49570943728605 40.0 38.0 41.0 34.0 41.0 32-33 38.27760880120434 40.0 38.0 41.0 34.0 41.0 34-35 38.181545334546776 40.0 38.0 41.0 34.0 41.0 36-37 38.131473967495666 40.0 38.0 41.0 34.0 41.0 38-39 37.95919537004731 40.0 37.5 41.0 33.0 41.0 40-41 37.76167455160753 40.0 37.0 41.0 33.0 41.0 42-43 37.50701885798079 40.0 37.0 41.0 33.0 41.0 44-45 37.21686458315543 40.0 37.0 41.0 32.0 41.0 46-47 36.82160067146026 40.0 36.0 41.0 31.0 41.0 48-49 36.41158244541336 39.0 36.0 41.0 30.0 41.0 50-51 36.05641461694803 39.0 35.0 40.5 29.5 41.0 52-53 35.56572502824183 39.0 35.0 40.0 28.0 41.0 54-55 35.22461394695171 38.0 35.0 40.0 27.0 41.0 56-57 34.874578446418596 38.0 34.0 40.0 26.0 41.0 58-59 34.416489082671106 38.0 34.0 40.0 25.5 41.0 60-61 33.957445474352994 37.0 33.0 40.0 23.5 41.0 62-63 33.43317604116598 36.5 33.0 39.5 22.5 41.0 64-65 32.8942330542902 36.0 32.0 39.0 21.0 40.0 66-67 32.34902635823087 35.5 31.0 39.0 18.5 40.0 68-69 31.751324011291857 35.0 31.0 38.0 15.0 40.0 70-71 31.155901018169928 35.0 30.0 37.5 10.5 40.0 72-73 30.50064120550539 34.5 29.5 37.0 7.5 39.0 74-75 29.85111759582094 34.0 29.0 36.0 4.5 39.0 76-77 28.510396166430404 32.5 27.0 35.0 2.0 37.0 78-79 29.187248110908058 34.0 29.0 35.0 2.0 37.5 80-81 28.89550765762359 34.0 29.0 35.0 2.0 37.0 82-83 28.351236906891007 34.0 27.5 35.0 2.0 36.5 84-85 27.70177307473655 33.0 26.0 35.0 2.0 36.0 86-87 27.010269779652702 33.0 25.5 35.0 2.0 36.0 88-89 26.233576916601805 32.0 24.5 35.0 2.0 35.0 90-91 25.599636699011597 32.0 21.5 34.0 2.0 35.0 92-93 24.683363059438385 31.0 18.0 34.0 2.0 35.0 94-95 23.807432664882285 31.0 4.5 34.0 2.0 35.0 96-97 22.791059965402226 31.0 2.0 34.0 2.0 35.0 98-99 21.401839196004424 30.0 2.0 34.0 2.0 35.0 100-101 17.48366731486655 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15270107228769803 1101 2 0.21004398118579815 1101 3 0.16011068204340262 1101 4 0.07280751780234596 1101 5 0.031302972122070116 1101 6 0.04120177563638805 1101 7 0.14710249048199842 1101 8 0.17426259527873356 1101 9 0.13950784864195498 1101 10-11 0.24847590409217446 1101 12-13 0.3052356086210324 1101 14-15 0.4177338057515456 1101 16-17 0.4638851018594323 1101 18-19 0.349258502475827 1101 20-21 0.2751471087028605 1101 22-23 0.2789677708084142 1101 24-25 0.32205701329890957 1101 26-27 0.3692419077459732 1101 28-29 0.4098603637262741 1101 30-31 0.507455876749141 1101 32-33 0.5391887857100031 1101 34-35 0.5961457944402824 1101 36-37 0.6275299591585153 1101 38-39 0.6772375979970562 1101 40-41 0.8127327430359017 1101 42-43 0.6690470439254099 1101 44-45 0.7915031457329746 1101 46-47 0.8622308663971481 1101 48-49 0.9308032173733167 1101 50-51 1.032837954698266 1101 52-53 1.0621570680773544 1101 54-55 1.212520023206764 1101 56-57 1.2909202183794761 1101 58-59 1.4151867306524295 1101 60-61 1.478577158545015 1101 62-63 1.3976956663518862 1101 64-65 1.4840234706683617 1101 66-67 1.635913951148666 1101 68-69 1.8323261657166192 1101 70-71 1.9176994497786346 1101 72-73 2.217315481123155 1101 74-75 2.122195839251713 1101 76-77 2.0165142842021666 1101 78-79 2.1916117039504215 1101 80-81 2.346751095581535 1101 82-83 2.582464043677689 1101 84-85 2.6912933920518505 1101 86-87 2.8690460246555993 1101 88-89 2.990526377308452 1101 90-91 3.314123518086056 1101 92-93 3.3407722766615784 1101 94-95 3.532770887206162 1101 96-97 3.914385110239614 1101 98-99 4.390998803840699 1101 100-101 4.095415752187485 1102 1 0.21522326180344464 1102 2 0.1584532114028505 1102 3 0.1987443283800019 1102 4 0.08109678285450883 1102 5 0.028136037821681725 1102 6 0.03839880765998771 1102 7 0.07436166480282935 1102 8 0.1536901465583682 1102 9 0.189688977240543 1102 10-11 0.17610273489717088 1102 12-13 0.3203497277953389 1102 14-15 0.31537789096861246 1102 16-17 0.3105878854832014 1102 18-19 0.3321210102154524 1102 20-21 0.2788069707141716 1102 22-23 0.3274351113371736 1102 24-25 0.33585937764279095 1102 26-27 0.36036474191327983 1102 28-29 0.3262221066316542 1102 30-31 0.36946894069301095 1102 32-33 0.5321535924582506 1102 34-35 0.44732551922425756 1102 36-37 0.4321117008675017 1102 38-39 0.7730963060370186 1102 40-41 0.6891323819440132 1102 42-43 0.794338663688201 1102 44-45 0.8196303234130227 1102 46-47 0.8263176449805414 1102 48-49 0.9619691448426337 1102 50-51 0.9561787615770356 1102 52-53 0.9639283061144397 1102 54-55 1.0753738610121886 1102 56-57 1.1051245382751844 1102 58-59 1.3679612261298502 1102 60-61 1.1012347808233258 1102 62-63 1.4365983059428729 1102 64-65 1.413580449460433 1102 66-67 1.5156599826727373 1102 68-69 1.7251442983091287 1102 70-71 1.636965271049025 1102 72-73 1.8480832985759825 1102 74-75 2.062056716328982 1102 76-77 2.206658332517634 1102 78-79 2.1862093094637522 1102 80-81 2.1930836978747195 1102 82-83 2.296254249914913 1102 84-85 2.3150087307658254 1102 86-87 2.397568437911584 1102 88-89 2.767549808660206 1102 90-91 2.920704860480871 1102 92-93 3.0801238339480115 1102 94-95 3.0713138332902012 1102 96-97 3.268179216393481 1102 98-99 3.7700586597923866 1102 100-101 3.5900024814392673 1103 1 0.26344486671279554 1103 2 0.2007495571842739 1103 3 0.18163478392131083 1103 4 0.09307771420745325 1103 5 0.020689050382394214 1103 6 0.06432086276696225 1103 7 0.15290631276194233 1103 8 0.0921082829152482 1103 9 0.27612888495602306 1103 10-11 0.2433143394105457 1103 12-13 0.30301859005186316 1103 14-15 0.3983594903708152 1103 16-17 0.41564253728931533 1103 18-19 0.26607876436387556 1103 20-21 0.31038764647197326 1103 22-23 0.3277662298274677 1103 24-25 0.34984736292754803 1103 26-27 0.4211412411099147 1103 28-29 0.4713733367831594 1103 30-31 0.42605532883508346 1103 32-33 0.49477352982363954 1103 34-35 0.4745913014794283 1103 36-37 0.39570010986767556 1103 38-39 0.3426186562626654 1103 40-41 0.4572789648120974 1103 42-43 0.46083259657303444 1103 44-45 0.3816072406581057 1103 46-47 0.5712684966797852 1103 48-49 0.6105795914766219 1103 50-51 0.6514325966847423 1103 52-53 0.7381930432485007 1103 54-55 0.8823132545222663 1103 56-57 0.9293701856926546 1103 58-59 0.9423386488982288 1103 60-61 0.9852670230095271 1103 62-63 1.1244716315967267 1103 64-65 1.2581723563650868 1103 66-67 1.4727449920323323 1103 68-69 1.4511492874274374 1103 70-71 1.4864652875777118 1103 72-73 1.8290458925397068 1103 74-75 1.86055703836578 1103 76-77 1.6845031816180551 1103 78-79 1.787681415410045 1103 80-81 2.023315088488829 1103 82-83 2.1378353942880253 1103 84-85 2.2650786779576038 1103 86-87 2.373102359665907 1103 88-89 2.639750967411455 1103 90-91 2.9709431459155446 1103 92-93 3.2590224499344806 1103 94-95 3.4841631923588743 1103 96-97 3.749905399020495 1103 98-99 4.157147072970137 1103 100-101 4.147359360330544 1104 1 0.188052692660996 1104 2 0.2349572996052629 1104 3 0.17302760675365647 1104 4 0.1244913663100462 1104 5 0.0316730101369842 1104 6 0.06484980277905805 1104 7 0.08766118469016249 1104 8 0.133481176102201 1104 9 0.07897117415323152 1104 10-11 0.16479219320771676 1104 12-13 0.2773679544373593 1104 14-15 0.37020533669781486 1104 16-17 0.33808551781868346 1104 18-19 0.3782201210375504 1104 20-21 0.32791719783497086 1104 22-23 0.3493406455473931 1104 24-25 0.4117364634799898 1104 26-27 0.3638710903341078 1104 28-29 0.40906446602247826 1104 30-31 0.30856711424894456 1104 32-33 0.4837582145313988 1104 34-35 0.5999550092996557 1104 36-37 0.7427676611873366 1104 38-39 0.7460688817270338 1104 40-41 0.751693673481391 1104 42-43 0.8323180676317108 1104 44-45 0.8868086482754975 1104 46-47 1.1931185784668443 1104 48-49 1.1299282565187028 1104 50-51 1.1774754227675643 1104 52-53 1.2921641318011368 1104 54-55 1.3133272958931812 1104 56-57 1.1993607363484315 1104 58-59 1.461262782943649 1104 60-61 1.510860693327885 1104 62-63 1.576369610198789 1104 64-65 1.6904306051698015 1104 66-67 1.8376945572245944 1104 68-69 1.8227129010825642 1104 70-71 2.024830019131624 1104 72-73 2.2309626516313408 1104 74-75 2.1396009669815363 1104 76-77 2.0272366096418253 1104 78-79 2.183009899962439 1104 80-81 2.327453297132184 1104 82-83 2.472589084227394 1104 84-85 2.676069171483917 1104 86-87 2.7910427834877076 1104 88-89 2.9294567638191253 1104 90-91 3.061999134757567 1104 92-93 3.516113069736601 1104 94-95 3.632847211935232 1104 96-97 3.9193676334916248 1104 98-99 3.9710614785906273 1104 100-101 3.7165579127881934 1105 1 0.19424304594904385 1105 2 0.17826009470859105 1105 3 0.1372010466534661 1105 4 0.13353829428338315 1105 5 0.006894672716640571 1105 6 -0.03559905298930488 1105 7 0.10941551805835559 1105 8 0.11800030626734781 1105 9 0.1477870616890513 1105 10-11 0.12320412868515973 1105 12-13 0.23706719205951998 1105 14-15 0.2687426870464904 1105 16-17 0.2703553523053799 1105 18-19 0.3019885329790739 1105 20-21 0.25841225081095587 1105 22-23 0.2540066964930645 1105 24-25 0.2684539472765195 1105 26-27 0.31493864899029944 1105 28-29 0.30805166946245066 1105 30-31 0.4243331882237129 1105 32-33 0.42122651930603894 1105 34-35 0.30614759900885957 1105 36-37 0.36577611304594626 1105 38-39 0.420938882192452 1105 40-41 0.31224084715884004 1105 42-43 0.25910625255836095 1105 44-45 0.396005779128501 1105 46-47 0.34298856382131504 1105 48-49 0.4741846434654988 1105 50-51 0.5369698465234336 1105 52-53 0.6157747249423338 1105 54-55 0.7598047512569437 1105 56-57 0.6438941675201519 1105 58-59 0.7679681477553117 1105 60-61 0.8072925740553174 1105 62-63 0.8873554548618117 1105 64-65 0.9324059332135022 1105 66-67 1.0784969730429808 1105 68-69 1.2915430835805743 1105 70-71 1.3166016817414992 1105 72-73 1.3472204360391267 1105 74-75 1.3949325647714623 1105 76-77 1.3667480060208774 1105 78-79 1.587630315827969 1105 80-81 1.671160703131477 1105 82-83 1.6421914062369822 1105 84-85 1.8331990634285091 1105 86-87 2.089024443625437 1105 88-89 2.11731342010356 1105 90-91 2.32826003202182 1105 92-93 2.569813621407299 1105 94-95 2.7494366817764906 1105 96-97 3.1189747184738152 1105 98-99 3.154098193977024 1105 100-101 2.9131402381705485 1106 1 0.08360001488728841 1106 2 0.10310779457918073 1106 3 0.09106853827560002 1106 4 0.1266909853131324 1106 5 0.038794932728052345 1106 6 -0.033797264534769056 1106 7 -0.010696019598853468 1106 8 0.07776007920325867 1106 9 0.13293997153182602 1106 10-11 0.15348693881414732 1106 12-13 0.09584034051105306 1106 14-15 0.13236676058134123 1106 16-17 0.07687449872946672 1106 18-19 0.027588744498324047 1106 20-21 -0.017131035133786554 1106 22-23 0.03876762332574657 1106 24-25 0.0997063725966072 1106 26-27 -0.06998735631022157 1106 28-29 0.11054998420332396 1106 30-31 0.08385229532984084 1106 32-33 0.1513014285755574 1106 34-35 0.17172503460005117 1106 36-37 0.1888716277779352 1106 38-39 0.10367068865917162 1106 40-41 0.11387573370371484 1106 42-43 -0.10782551595797685 1106 44-45 -0.03247284927856242 1106 46-47 9.516197502605905E-4 1106 48-49 -0.01291308105005129 1106 50-51 0.06769099497267916 1106 52-53 0.16184654689691058 1106 54-55 0.22472970807840653 1106 56-57 0.31266795417631954 1106 58-59 0.34815029819723975 1106 60-61 0.3999946470237603 1106 62-63 0.3445883363044757 1106 64-65 0.34745405783207417 1106 66-67 0.5404242199934188 1106 68-69 0.5899594528457861 1106 70-71 0.4718659013112436 1106 72-73 0.7777463052652998 1106 74-75 0.916122240899707 1106 76-77 0.9465738730247324 1106 78-79 0.9037444701241988 1106 80-81 0.6184948596728148 1106 82-83 1.0949014396686465 1106 84-85 1.229013010821074 1106 86-87 1.4761814584916166 1106 88-89 1.5103216888424136 1106 90-91 1.5318802193462773 1106 92-93 1.7057965106850617 1106 94-95 2.045977470175753 1106 96-97 2.6924746669046016 1106 98-99 2.8437421270726624 1106 100-101 2.748008349193082 1107 1 0.1793077042798359 1107 2 0.1749565708562315 1107 3 0.1534536428408373 1107 4 0.1042709314819561 1107 5 0.060054258719155484 1107 6 0.0909025806645829 1107 7 0.05973716735533685 1107 8 0.15099231009446612 1107 9 0.14034535974680296 1107 10-11 0.20842897998868182 1107 12-13 0.24311204833784217 1107 14-15 0.37012482168382377 1107 16-17 0.2880469058740118 1107 18-19 0.2525015098451391 1107 20-21 0.23627524398829536 1107 22-23 0.32868031664649777 1107 24-25 0.32151204404025435 1107 26-27 0.3810110912509188 1107 28-29 0.23557782505009328 1107 30-31 0.2904817965437516 1107 32-33 0.32619679685594605 1107 34-35 0.3768558394858559 1107 36-37 0.47344552708105425 1107 38-39 0.44887004020291954 1107 40-41 0.42007407577436595 1107 42-43 0.42836924697097345 1107 44-45 0.5440127438450588 1107 46-47 0.5468374905620763 1107 48-49 0.47673815763216254 1107 50-51 0.4402890640091215 1107 52-53 0.5886639031855125 1107 54-55 0.6488427624159812 1107 56-57 0.6644853935719226 1107 58-59 0.7150704966346098 1107 60-61 0.7611862795888555 1107 62-63 0.9631252430174158 1107 64-65 0.9960920448252679 1107 66-67 1.317218472534556 1107 68-69 1.427281922431387 1107 70-71 1.1954292859160596 1107 72-73 1.4669906520497769 1107 74-75 1.3348610422307452 1107 76-77 1.361674526689029 1107 78-79 1.4002664419542477 1107 80-81 1.5624904339477652 1107 82-83 1.7780219082591842 1107 84-85 1.7662036726963457 1107 86-87 2.027842435098659 1107 88-89 2.097330567994856 1107 90-91 2.441206044238708 1107 92-93 2.568780761353519 1107 94-95 2.652605420058908 1107 96-97 2.7220344435572947 1107 98-99 2.9949744377465954 1107 100-101 3.1862592902414058 1108 1 0.1726886076365659 1108 2 0.1987231296145211 1108 3 0.10369102474891889 1108 4 0.14077277209373307 1108 5 0.02167379878378739 1108 6 0.1103858543150551 1108 7 0.11928177086227976 1108 8 0.09121800881361253 1108 9 0.1757879465954275 1108 10-11 0.1557414334087781 1108 12-13 0.1866351770308441 1108 14-15 0.37441733326097904 1108 16-17 0.31730156138518595 1108 18-19 0.24680354534199012 1108 20-21 0.2822649246643252 1108 22-23 0.24013485438936044 1108 24-25 0.15634460978643006 1108 26-27 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-0.3915657053511197 2316 50-51 -0.29328182810164094 2316 52-53 -0.21723179953131222 2316 54-55 -0.03738576749520206 2316 56-57 -0.014898014865906362 2316 58-59 -0.027010877451076 2316 60-61 0.10141167470379742 2316 62-63 0.23591170135075146 2316 64-65 0.5360015112568277 2316 66-67 0.6990370625293281 2316 68-69 0.9338964241293972 2316 70-71 1.0416875310832516 2316 72-73 1.177981215006934 2316 74-75 1.5222814477950095 2316 76-77 1.4666310150641593 2316 78-79 1.4021216045718035 2316 80-81 1.4042852680647968 2316 82-83 1.6171860442309764 2316 84-85 1.960434504723512 2316 86-87 1.8862561792209114 2316 88-89 2.209344429671976 2316 90-91 2.474125486506214 2316 92-93 2.6039451748853892 2316 94-95 3.0741314290714463 2316 96-97 3.6096062826377526 2316 98-99 3.998109233984838 2316 100-101 4.644866101892401 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 8.0 5 36.0 6 81.0 7 202.0 8 464.0 9 860.0 10 1515.0 11 2491.0 12 3927.0 13 6187.0 14 8182.0 15 11311.0 16 15551.0 17 22234.0 18 22988.0 19 17106.0 20 15939.0 21 17152.0 22 19215.0 23 21328.0 24 23850.0 25 27154.0 26 31482.0 27 36001.0 28 43161.0 29 52930.0 30 62560.0 31 75747.0 32 92592.0 33 116826.0 34 154074.0 35 210953.0 36 296982.0 37 394938.0 38 232853.0 39 10375.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.91982735273378 19.710237572983484 15.369608127792159 22.00032694649057 2 15.272847068010446 20.506210498059982 40.68044608213963 23.54049635178994 3 19.22334048404948 25.31685350271226 30.070583229065612 25.38922278417265 4 12.739468930159836 17.335652262175596 37.507990837498326 32.416887970166236 5 13.271791013420753 38.01898545555811 34.091204447853514 14.61801908316763 6 30.807158082121187 36.88011891098463 17.5330264914623 14.779696515431882 7 28.066013912305145 33.53880537656184 20.450600581183988 17.94458012994903 8 25.964199829792552 35.39075492469492 19.41897188454001 19.226073360972524 9 26.360475585148823 17.200508474989814 20.730701007434373 35.708314932426994 10-11 25.00674632124201 27.20763298060524 26.64552900674144 21.14009169141131 12-13 25.919585802714636 24.651009020307278 27.649547520696444 21.779857656281642 14-15 22.852125726083674 25.82346007555394 26.480769075929437 24.84364512243295 16-17 21.449036959357855 29.310784430218746 27.122096732754606 22.118081877668793 18-19 21.40379599514948 27.567713302086354 29.99146035285822 21.03703034990594 20-21 22.374802673153546 26.4718813818613 30.07885754160638 21.074458403378774 22-23 22.110929907312794 26.3325310917415 30.000151274266102 21.556387726679606 24-25 21.89770336161026 26.606363480209406 30.00364522538912 21.492287932791218 26-27 21.565760144841782 26.75312601796126 30.293526650912035 21.38758718628493 28-29 21.33374648959505 26.909379704430613 30.315015383564354 21.441858422409986 30-31 21.61294415314759 26.577504617509206 30.093106552837234 21.716444676505965 32-33 21.379665392225995 26.7353172966893 30.013370647524845 21.87164666355986 34-35 21.938259084613378 26.771120158281008 29.888473187591693 21.40214756951392 36-37 21.792938005154078 26.56375426924276 30.05146758498593 21.59184014061723 38-39 21.54489696522497 26.955967310749912 29.865712693388456 21.633423030636656 40-41 21.763158536882944 26.811546814377397 29.771064639711085 21.654230009028574 42-43 21.518180580410192 26.79186408717426 29.622445936996893 22.067509395418657 44-45 21.729051091755906 26.85437951644954 29.483509469172 21.933059922622554 46-47 21.88460786784722 27.19158507845132 29.02760966190553 21.896197391795937 48-49 21.819506018011335 27.121358145481427 29.07544900244722 21.983686834060016 50-51 21.65483722212598 27.308449614861914 28.989442556233573 22.047270606778525 52-53 22.036730242306387 27.463041627119967 28.48413943535853 22.016088695215114 54-55 21.823458654688388 27.245500215931077 28.660104964462867 22.270936164917664 56-57 21.789115506835625 27.467227258176596 28.583091295216235 22.160565939771548 58-59 22.049588511002725 27.169033775524394 28.604450864090296 22.176926849382582 60-61 22.00645109344082 27.318916782358553 28.84829438847587 21.82633773572476 62-63 21.822702285941546 27.382646949028068 28.742573556860627 22.05207720816976 64-65 21.598231561071895 27.304960559029702 28.69360478025048 22.40320309964792 66-67 21.734914713324045 27.33789919800514 28.30849109314803 22.618694995522784 68-69 21.571995505656357 27.24457939946102 28.594590440126044 22.588834654756575 70-71 21.846735292897698 27.20982888987027 28.49814445667105 22.445291360560983 72-73 21.899076473534773 27.13156354389587 28.291979878634503 22.67738010393485 74-75 21.551117107841105 27.621879063957078 28.122497578400065 22.70450624980176 76-77 21.538112445193764 27.256772550158225 28.42060446062369 22.78451054402432 78-79 21.77144000409903 27.305960917694883 28.36477956730828 22.557819510897808 80-81 21.66333864255655 27.486063289063782 28.295939420201595 22.554658648178076 82-83 21.688787733647338 27.405829206347505 28.37419978523489 22.53118327477027 84-85 21.763197384313198 27.221442775751214 28.23065379969988 22.784706040235704 86-87 21.50805901043189 27.27031612870798 28.351886129054453 22.86973873180568 88-89 21.52122834284487 27.273168672670444 28.144481070042186 23.061121914442495 90-91 21.68583831981753 27.117307874366954 28.333095842975283 22.863757962840243 92-93 21.82260468997421 27.01070871751579 28.190643962591466 22.97604262991853 94-95 21.512083969638862 27.01557070841758 28.166373838028232 23.30597148391533 96-97 21.403151090223343 27.010007031810034 28.28173500762959 23.305106870337035 98-99 21.35956062295506 26.905915047590234 28.05444879017599 23.680075539278718 100-101 21.558875987244207 26.478835094533892 28.108760945997712 23.853527972224185 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1470.0 1 966.0 2 645.5 3 846.0 4 706.5 5 659.0 6 1293.0 7 2893.0 8 4669.5 9 5449.5 10 4703.0 11 3541.5 12 3247.5 13 3357.5 14 3448.5 15 3471.0 16 3481.5 17 3567.0 18 3715.5 19 3928.0 20 4232.0 21 4762.0 22 5523.5 23 6484.5 24 7752.5 25 9362.0 26 11785.0 27 14571.0 28 16881.5 29 19730.0 30 23455.0 31 27402.0 32 31230.5 33 35612.5 34 40421.0 35 44982.5 36 51358.0 37 56991.5 38 59638.0 39 61160.0 40 62524.5 41 65069.5 42 67602.0 43 70225.5 44 73649.5 45 76092.5 46 75399.5 47 77217.5 48 83548.5 49 82881.0 50 76390.5 51 71092.5 52 66408.5 53 63802.0 54 61761.5 55 57581.0 56 52019.0 57 46998.0 58 43045.0 59 38319.0 60 32159.5 61 26011.5 62 20692.0 63 16005.0 64 12697.0 65 10234.0 66 7758.5 67 6215.5 68 5097.5 69 3575.0 70 2364.5 71 1664.5 72 1236.5 73 985.0 74 758.0 75 581.0 76 405.0 77 223.0 78 129.5 79 87.5 80 44.5 81 26.5 82 11.0 83 4.0 84 5.5 85 3.5 86 1.0 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015371364855204184 3 0.002586293134367688 4 0.0020983132976945392 5 0.1188230902299117 6 4.8797983667314866E-5 7 0.0 8 4.8797983667314866E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 2.4398991833657433E-5 16-17 2.4398991833657433E-5 18-19 0.0 20-21 0.0 22-23 3.4158588567120406E-4 24-25 4.391818530058338E-4 26-27 0.00646573283591922 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01224829390049603 36-37 6.831717713424081E-4 38-39 0.007368495533764546 40-41 0.010491566488472697 42-43 0.006246141909416303 44-45 0.004343020546391023 46-47 2.4398991833657433E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.8797983667314866E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0010979546325145845 70-71 0.0 72-73 2.4398991833657433E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.8797983667314866E-5 82-83 0.002415500191532086 84-85 0.004391818530058338 86-87 0.003318262889377411 88-89 0.0 90-91 0.002879081036371577 92-93 0.0 94-95 2.4398991833657433E-5 96-97 2.927879020038892E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2049265.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.64333583210392 #Duplication Level Percentage of deduplicated Percentage of total 1 73.26478984868031 34.90578987429195 2 14.132278209905142 13.466177536544702 3 4.992264476117237 7.135443989950076 4 2.336779275798932 4.453278392095564 5 1.3076085477914727 3.1149416589679424 6 0.7856717253480348 2.245921311872696 7 0.5522857140454619 1.841891362467888 8 0.3768534058602197 1.4363642699896484 9 0.2933044821533252 1.2576603549862984 >10 1.7166944435020413 15.387013648164539 >50 0.16041533529911506 5.287368066413461 >100 0.07705921610484816 6.2940331502566895 >500 0.002765990349544537 0.935010649489684 >1k 9.219967831815118E-4 0.8336856973395254 >5k 1.0244408702016799E-4 0.3276954377341826 >10k+ 2.0488817404033598E-4 1.0777245994352824 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11856 0.578548894359685 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10225 0.4989593829982945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6714 0.327629662342352 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3806 0.18572512583780038 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2728 0.13312089944443495 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2191 0.10691638221508688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.8797983667314866E-5 0.0 20-21 0.0 0.0 0.0 1.7079294283560203E-4 0.0 22-23 0.0 0.0 0.0 4.8797983667314866E-4 0.0 24-25 0.0 0.0 0.0 0.0010491566488472696 0.0 26-27 0.0 0.0 0.0 0.0020007173303599095 0.0 28-29 0.0 0.0 0.0 0.005172586268735376 0.0 30-31 0.0 0.0 0.0 0.015834945700043675 0.0 32-33 0.0 0.0 0.0 0.03528094219146865 0.0 34-35 0.0 0.0 0.0 0.06634085879571457 0.0 36-37 0.0 0.0 0.0 0.11365050396117632 0.0 38-39 0.0 0.0 0.0 0.18484676213178872 0.0 40-41 0.0 0.0 0.0 0.26163038943230865 0.0 42-43 0.0 0.0 0.0 0.3362913044433004 0.0 44-45 0.0 0.0 0.0 0.4273239429746763 0.0 46-47 0.0 0.0 0.0 0.5345819110754344 0.0 48-49 0.0 0.0 0.0 0.649061980758955 0.0 50-51 0.0 0.0 0.0 0.7690562225968823 0.0 52-53 0.0 0.0 0.0 0.885097827757757 0.0 54-55 0.0 0.0 0.0 1.0117773933581065 0.0 56-57 0.0 0.0 0.0 1.1573417786377067 0.0 58-59 0.0 0.0 0.0 1.3123241747650987 0.0 60-61 0.0 0.0 0.0 1.4740650916304139 0.0 62-63 0.0 0.0 0.0 1.6351472357162202 0.0 64-65 0.0 0.0 0.0 1.8025243196951102 0.0 66-67 0.0 0.0 0.0 1.9864683191290538 0.0 68-69 0.0 0.0 0.0 2.19098066867877 0.0 70-71 0.0 0.0 0.0 2.409156453655335 0.0 72-73 0.0 0.0 0.0 2.628113006370577 0.0 74-75 0.0 0.0 0.0 2.8466547762246464 0.0 76-77 0.0 0.0 0.0 3.083105406084621 0.0 78-79 0.0 0.0 0.0 3.3445162045904264 0.0 80-81 0.0 0.0 0.0 3.6232746862899625 0.0 82-83 0.0 0.0 0.0 3.899715263765301 0.0 84-85 0.0 0.0 0.0 4.175521467452965 0.0 86-87 0.0 0.0 0.0 4.461990030571936 0.0 88-89 0.0 0.0 0.0 4.78115324274801 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9945 0.0 30.896223 1 GTATCAA 14005 0.0 28.90175 1 GTACATG 10875 0.0 25.498636 1 TACATGG 10940 0.0 24.781935 2 TATCAAC 16970 0.0 23.711893 2 ATCAACG 16890 0.0 23.708662 3 CAACGCA 17105 0.0 23.43789 5 TCAACGC 17175 0.0 23.342934 4 GAGTACT 8945 0.0 23.25544 12-13 ACATGGG 11315 0.0 23.24316 3 AACGCAG 17425 0.0 23.031103 6 GTACTTT 9260 0.0 22.233555 14-15 AGTACTT 9290 0.0 21.215986 12-13 ACGCAGA 19180 0.0 20.874197 7 CGCAGAG 19245 0.0 20.72966 8 AGAGTAC 15685 0.0 20.211452 10-11 CATGGGA 5640 0.0 19.900204 4 GCAGAGT 20065 0.0 19.787819 9 ATTCGCG 200 1.8189894E-12 18.999107 80-81 CATGGGG 5090 0.0 18.873749 4 >>END_MODULE