FastQCFastQC Report
Thu 26 May 2016
SRR938278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032174
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125020.6152032257080349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103820.5108814501120474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65430.32197046119082323No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37450.18428540075800595No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.12995934403254839No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21290.10476465105842316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99100.031.4148651
GTATCAA140700.029.9998761
GTACATG109600.024.9199261
ATCAACG169750.024.86153
TATCAAC171450.024.5872692
CAACGCA172200.024.5353785
TCAACGC173450.024.358564
TACATGG111300.024.168262
GAGTACT88200.024.10068312-13
AACGCAG176250.024.0489256
GGACCGA6100.023.3696066
ACATGGG114650.022.757393
GTACTTT93400.022.60770814-15
ACGCAGA193700.021.8263637
CGCAGAG195150.021.566828
AGTACTT92150.020.97992912-13
GCAGAGT201200.020.4456169
AGAGTAC157600.020.41985710-11
AGGACCG7950.020.3252535
CATGGGA55350.020.1778264
Produced by FastQC (version 0.11.5)