##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938278_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2032174 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97487223042909 33.0 31.0 34.0 30.0 34.0 2 32.15927917589734 34.0 31.0 34.0 30.0 34.0 3 32.237229193956814 34.0 31.0 34.0 30.0 34.0 4 35.75132050700383 37.0 35.0 37.0 35.0 37.0 5 35.768726004761405 37.0 35.0 37.0 35.0 37.0 6 35.80549500190436 37.0 36.0 37.0 35.0 37.0 7 35.75250347657238 37.0 36.0 37.0 35.0 37.0 8 35.736808954351346 37.0 36.0 37.0 35.0 37.0 9 37.38073855880451 39.0 37.0 39.0 35.0 39.0 10-11 37.2985854065646 39.0 37.0 39.0 34.0 39.0 12-13 37.198630383028224 39.0 37.0 39.0 34.0 39.0 14-15 38.39249001315832 40.0 38.0 41.0 34.0 41.0 16-17 38.266153636450426 40.0 38.0 41.0 33.0 41.0 18-19 38.431596162533324 40.0 38.0 41.0 34.0 41.0 20-21 38.424910711385934 40.0 38.0 41.0 34.0 41.0 22-23 38.34257721041604 40.0 38.0 41.0 34.0 41.0 24-25 38.24595925348912 40.0 38.0 41.0 34.0 41.0 26-27 38.08314371702423 40.0 38.0 41.0 33.0 41.0 28-29 37.88339458136951 40.0 38.0 41.0 33.0 41.0 30-31 37.67920955587464 40.0 37.5 41.0 33.0 41.0 32-33 37.43247723866165 40.0 37.0 41.0 32.0 41.0 34-35 37.21519909220372 40.0 36.5 41.0 31.5 41.0 36-37 36.97627614564501 39.5 36.0 41.0 31.0 41.0 38-39 36.7162602710201 39.0 36.0 41.0 30.0 41.0 40-41 36.5191110603718 39.0 35.0 41.0 30.0 41.0 42-43 36.34711127098369 39.0 35.0 41.0 30.0 41.0 44-45 36.069604276011795 39.0 35.0 41.0 29.5 41.0 46-47 35.66542604127403 39.0 35.0 40.5 27.5 41.0 48-49 35.284251742222864 39.0 35.0 40.0 26.0 41.0 50-51 34.59079143813473 38.5 34.0 39.5 24.5 40.5 52-53 34.72153836236464 38.0 34.5 40.0 26.0 40.5 54-55 34.851459815940956 38.0 34.5 40.0 25.5 41.0 56-57 34.5934649296763 38.0 34.0 40.0 24.0 41.0 58-59 34.305519852138644 38.0 34.0 40.0 23.0 41.0 60-61 33.90851078697001 38.0 34.0 40.0 22.5 41.0 62-63 33.396735958633464 37.0 33.0 40.0 20.5 41.0 64-65 32.903266403369 36.5 32.5 39.0 18.5 41.0 66-67 32.387902069409414 36.0 31.5 39.0 15.0 40.5 68-69 31.804620322866054 35.0 31.0 38.5 9.5 40.0 70-71 31.289156341927416 35.0 31.0 38.0 7.0 40.0 72-73 30.654649158979495 35.0 30.0 37.0 2.0 39.5 74-75 29.978949883228502 34.0 29.0 37.0 2.0 39.0 76-77 29.327816171253055 34.0 28.5 36.0 2.0 39.0 78-79 28.61753693335315 34.0 27.5 35.5 2.0 37.5 80-81 27.955321247097935 33.5 26.0 35.0 2.0 37.0 82-83 27.243800235609747 33.0 25.5 35.0 2.0 36.5 84-85 26.588148701833603 32.5 24.5 35.0 2.0 36.0 86-87 25.91742193335807 32.0 22.0 35.0 2.0 35.5 88-89 25.124213280949366 31.5 19.0 34.0 2.0 35.0 90-91 24.316187491819107 31.0 13.5 34.0 2.0 35.0 92-93 23.429664487391335 31.0 4.5 34.0 2.0 35.0 94-95 22.498158868285884 30.0 2.0 34.0 2.0 35.0 96-97 21.30024988017758 29.0 2.0 34.0 2.0 35.0 98-99 19.818485031301456 28.0 2.0 34.0 2.0 35.0 100-101 16.028254962419556 13.5 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24411589335130657 1101 2 0.2054157964778014 1101 3 0.17067594167319555 1101 4 0.07703552679678438 1101 5 0.1795317163017458 1101 6 0.1254178419638805 1101 7 0.09525070728064122 1101 8 0.07478832080781928 1101 9 0.16071276177930116 1101 10-11 0.18728476895909552 1101 12-13 0.26030519600193003 1101 14-15 0.20191485624273042 1101 16-17 0.4527438623950033 1101 18-19 0.3177016208606034 1101 20-21 0.3258683969475058 1101 22-23 0.3981183462111346 1101 24-25 0.38632831970740966 1101 26-27 0.5285203096218325 1101 28-29 0.5657906023289954 1101 30-31 0.5907006230220588 1101 32-33 0.4259851536912933 1101 34-35 0.48900228191366324 1101 36-37 0.5640804087509181 1101 38-39 0.706704732237597 1101 40-41 0.6588496101657455 1101 42-43 0.7931484256739267 1101 44-45 0.8251245844971251 1101 46-47 0.8573043545818209 1101 48-49 0.9286036122180548 1101 50-51 0.8227708624560819 1101 52-53 0.7887792726035343 1101 54-55 0.9342460396481087 1101 56-57 0.9559267483258296 1101 58-59 1.0529395006316236 1101 60-61 1.0343410777476336 1101 62-63 0.8189361250312714 1101 64-65 0.86859746169619 1101 66-67 0.9700413724734176 1101 68-69 1.2877414317686373 1101 70-71 1.5034573993891094 1101 72-73 1.5469756536124954 1101 74-75 1.764565516461733 1101 76-77 1.761972319231237 1101 78-79 1.8135658322242527 1101 80-81 1.9766376130538994 1101 82-83 2.2644424063596524 1101 84-85 2.5460521654285806 1101 86-87 2.6263814192495616 1101 88-89 2.9340104108463834 1101 90-91 3.1008592025348385 1101 92-93 3.439866850619829 1101 94-95 3.6055001065316894 1101 96-97 3.600085045529429 1101 98-99 4.1727373500555025 1101 100-101 4.144858499279934 1102 1 0.19428036258128856 1102 2 0.18294597347404817 1102 3 0.15440435530376106 1102 4 0.09568544169776061 1102 5 0.0809282985915658 1102 6 0.1442965624856214 1102 7 0.019378902313299307 1102 8 0.08564322054651541 1102 9 0.18718130152724655 1102 10-11 0.1821706682137929 1102 12-13 0.3115206781221005 1102 14-15 0.317756642190389 1102 16-17 0.4007884944207021 1102 18-19 0.3162300083816305 1102 20-21 0.3329617183926601 1102 22-23 0.43892647099804805 1102 24-25 0.22936523344943538 1102 26-27 0.4012540402922653 1102 28-29 0.3585236108208463 1102 30-31 0.43772479586717594 1102 32-33 0.29960459473894474 1102 34-35 0.18080145793567226 1102 36-37 0.2972013938025171 1102 38-39 0.2645458557252738 1102 40-41 0.5339097627815264 1102 42-43 0.5835727194839606 1102 44-45 0.5602124646043691 1102 46-47 0.5830676244398276 1102 48-49 0.8193522267114801 1102 50-51 0.8665176985847367 1102 52-53 0.7075314035511795 1102 54-55 0.8718978967928521 1102 56-57 0.849557827559245 1102 58-59 0.8462771600924768 1102 60-61 0.9394041051897304 1102 62-63 0.980959322240885 1102 64-65 1.0126292111668889 1102 66-67 1.274821222579689 1102 68-69 1.4160522560452264 1102 70-71 1.4849927163525969 1102 72-73 1.6061939508403675 1102 74-75 1.428534603051638 1102 76-77 1.6236570646667552 1102 78-79 1.7713843529108466 1102 80-81 1.9092144295934546 1102 82-83 2.101512319496848 1102 84-85 2.2293780686552616 1102 86-87 2.2963951059226595 1102 88-89 2.595804628834607 1102 90-91 2.826042125511023 1102 92-93 2.9777800114219204 1102 94-95 3.2765408144889143 1102 96-97 3.592141254633461 1102 98-99 3.7638061535375122 1102 100-101 4.041139592019469 1103 1 0.47734099915668793 1103 2 0.5061685381085965 1103 3 0.44713379167853873 1103 4 0.26342373692052234 1103 5 0.33821844903232545 1103 6 0.39198683691131464 1103 7 0.3769477073306433 1103 8 0.36232976997265354 1103 9 0.38476203775787354 1103 10-11 0.5222649575083338 1103 12-13 0.5609408616540676 1103 14-15 0.6944732143496992 1103 16-17 0.7146208748924536 1103 18-19 0.636343409423823 1103 20-21 0.6456128542332635 1103 22-23 0.6482221088623561 1103 24-25 0.7502837966883646 1103 26-27 0.7262472063238263 1103 28-29 0.7310313540380804 1103 30-31 0.7610715790734517 1103 32-33 0.8163258163331975 1103 34-35 0.8449459813763767 1103 36-37 0.8889884045117995 1103 38-39 1.0438439156570354 1103 40-41 1.0572357909725199 1103 42-43 1.3022525058124401 1103 44-45 1.1436604762116005 1103 46-47 1.172019219694441 1103 48-49 1.3031056836091892 1103 50-51 1.0728632427204943 1103 52-53 1.2970676827727132 1103 54-55 1.4593549751876225 1103 56-57 1.4633899354510547 1103 58-59 1.4658865997693624 1103 60-61 1.4960314191013495 1103 62-63 1.5521626536350013 1103 64-65 1.669581712944499 1103 66-67 1.723383830686842 1103 68-69 1.7823917099663618 1103 70-71 1.9167490189848628 1103 72-73 1.9973866594049134 1103 74-75 2.116590422750132 1103 76-77 2.148123450498204 1103 78-79 2.3789389448314218 1103 80-81 2.311690974089796 1103 82-83 2.481037438516932 1103 84-85 2.593967948774605 1103 86-87 2.723033330819799 1103 88-89 2.961512086699585 1103 90-91 3.298392395404104 1103 92-93 3.5323538249009196 1103 94-95 3.6872319448156183 1103 96-97 3.894577067899071 1103 98-99 4.02718690799205 1103 100-101 3.8050033970640698 1104 1 0.24573619036529237 1104 2 0.15871855636815013 1104 3 0.22368782634166706 1104 4 0.19668628975277613 1104 5 0.19636755173062426 1104 6 0.17090413963656204 1104 7 0.1932635787582484 1104 8 0.11574619184026602 1104 9 0.23234887886903266 1104 10-11 0.22296974835439443 1104 12-13 0.1916136302004361 1104 14-15 0.29323610339514516 1104 16-17 0.3800842032145937 1104 18-19 0.3000036504499306 1104 20-21 0.2904945581603613 1104 22-23 0.23049309460594003 1104 24-25 0.23481467279084 1104 26-27 0.35275030276708463 1104 28-29 0.31947759518848073 1104 30-31 0.2837067922912624 1104 32-33 0.2391392260377856 1104 34-35 0.26575694995668186 1104 36-37 0.2821200890621185 1104 38-39 0.41784671010194074 1104 40-41 0.48589714572667475 1104 42-43 0.3698765712759027 1104 44-45 0.507260911089233 1104 46-47 0.5455632559426249 1104 48-49 0.7567747716160511 1104 50-51 0.8611395268768476 1104 52-53 0.7892270722681971 1104 54-55 0.8013396595068158 1104 56-57 0.953627358528756 1104 58-59 0.8622053270889438 1104 60-61 0.8685235235027093 1104 62-63 0.8658710398325908 1104 64-65 1.0627938722013184 1104 66-67 1.2433892142876033 1104 68-69 1.470962625813911 1104 70-71 1.4660847273403341 1104 72-73 1.3692538192308241 1104 74-75 1.465295879738239 1104 76-77 1.5502955343985825 1104 78-79 1.9209736247166553 1104 80-81 2.1386595320458994 1104 82-83 2.4105150616606856 1104 84-85 2.466616987581915 1104 86-87 2.5035110747628977 1104 88-89 2.793464664927633 1104 90-91 2.8413646667180252 1104 92-93 3.031067626121061 1104 94-95 3.1531166044861614 1104 96-97 3.4295750115172012 1104 98-99 3.7155284447412384 1104 100-101 4.098530612178372 1105 1 0.27336228229322046 1105 2 0.21974408583233895 1105 3 0.1866925934110597 1105 4 0.1088880326337005 1105 5 0.07714457563322696 1105 6 0.20606337825066845 1105 7 0.24641282922696917 1105 8 0.14121049767257432 1105 9 0.2480777906824514 1105 10-11 0.15051891138480045 1105 12-13 0.14078984992281818 1105 14-15 0.3603991296014186 1105 16-17 0.3553442586202138 1105 18-19 0.28897559872680745 1105 20-21 0.359377449678 1105 22-23 0.2573086481156679 1105 24-25 0.18424627123039983 1105 26-27 0.33013956509042686 1105 28-29 0.31970880225753007 1105 30-31 0.3765109451906099 1105 32-33 0.3811849672035734 1105 34-35 0.4668637487056344 1105 36-37 0.48457655907666464 1105 38-39 0.5628797516939983 1105 40-41 0.7100647477310318 1105 42-43 0.7891939744421776 1105 44-45 0.9156811047146434 1105 46-47 0.8989979420475009 1105 48-49 0.9585473416297532 1105 50-51 0.9656083391451133 1105 52-53 0.7826098208578358 1105 54-55 0.8242338885783767 1105 56-57 1.065879704229566 1105 58-59 1.1460545674686244 1105 60-61 1.0845376607640347 1105 62-63 1.190108476881214 1105 64-65 1.2836462801178499 1105 66-67 1.5076318324764486 1105 68-69 1.5109582959360779 1105 70-71 1.576207340652818 1105 72-73 1.7702267806150367 1105 74-75 1.7875425713752655 1105 76-77 1.8724421238841273 1105 78-79 2.0636646437933415 1105 80-81 2.2319526353492165 1105 82-83 2.226977372806548 1105 84-85 2.2773198780906583 1105 86-87 2.4315744107866415 1105 88-89 2.657512543707874 1105 90-91 2.6720769526413015 1105 92-93 2.95558140816426 1105 94-95 3.0998478094533333 1105 96-97 3.271650073943107 1105 98-99 3.495395668827129 1105 100-101 3.5320152285007467 1106 1 0.13292247428068293 1106 2 0.12342588620455786 1106 3 0.08329118289092463 1106 4 0.17362870263076502 1106 5 0.21324997283178249 1106 6 -0.04027563634429754 1106 7 0.07479954682908385 1106 8 0.10922891279648184 1106 9 0.016682698117065797 1106 10-11 0.10722990168748225 1106 12-13 0.27104562913724095 1106 14-15 0.2945687348516941 1106 16-17 0.15841936391945666 1106 18-19 0.27706209103751434 1106 20-21 0.09401110625552889 1106 22-23 0.07870704658759564 1106 24-25 -0.007346318641289429 1106 26-27 -0.09167268390556416 1106 28-29 0.02558719387277364 1106 30-31 0.011884757513065836 1106 32-33 0.1692518689569269 1106 34-35 0.17284315413222373 1106 36-37 0.09285112073794011 1106 38-39 -0.036830063175237626 1106 40-41 0.2712944225277809 1106 42-43 0.18755265305908608 1106 44-45 0.3372974481371145 1106 46-47 0.5010831119955839 1106 48-49 0.5536258263001486 1106 50-51 0.5777336697357711 1106 52-53 0.6520265120127036 1106 54-55 0.1764170113132124 1106 56-57 0.5083599912312309 1106 58-59 0.6669491212936833 1106 60-61 0.6414788401155889 1106 62-63 0.5355757116740492 1106 64-65 0.787530720317676 1106 66-67 0.9284339433590034 1106 68-69 0.6499471782947808 1106 70-71 0.6989697316803465 1106 72-73 1.0458965327944831 1106 74-75 1.2892169518238923 1106 76-77 1.4853755649655263 1106 78-79 1.5722600008312497 1106 80-81 1.612597164686079 1106 82-83 1.7376511655384554 1106 84-85 1.8779663664832498 1106 86-87 1.7951904603507494 1106 88-89 2.0829000694204787 1106 90-91 2.2993115005891873 1106 92-93 2.6307118078116325 1106 94-95 2.753661233882756 1106 96-97 2.8896035953457897 1106 98-99 3.582794042810871 1106 100-101 3.8558195922973972 1107 1 0.21781747826630493 1107 2 0.13694728587238814 1107 3 0.05812966519532381 1107 4 0.1106203927450835 1107 5 -0.002627273719561174 1107 6 0.06858614902550642 1107 7 0.08010000018204977 1107 8 0.05009755077806233 1107 9 0.03208062330907069 1107 10-11 0.07121932693940636 1107 12-13 0.08237835939507221 1107 14-15 0.29099712962452884 1107 16-17 0.1604181216219942 1107 18-19 0.09682468000065825 1107 20-21 0.14367803545979285 1107 22-23 0.21774924094532366 1107 24-25 0.21032398360036808 1107 26-27 0.22187235365824165 1107 28-29 0.2701705723892118 1107 30-31 0.3235057083938031 1107 32-33 0.2600750146900239 1107 34-35 0.26203328747468646 1107 36-37 0.4230468444190265 1107 38-39 0.41513304743870094 1107 40-41 0.4514716686986233 1107 42-43 0.4601508620960928 1107 44-45 0.39754090895338123 1107 46-47 0.5196767903565913 1107 48-49 0.6623345836412113 1107 50-51 0.4508063726744851 1107 52-53 0.43814552620305136 1107 54-55 0.5831253225306128 1107 56-57 0.6140828590169107 1107 58-59 0.6530405737924525 1107 60-61 0.5822290508115273 1107 62-63 0.6878270666914617 1107 64-65 0.8164211517734898 1107 66-67 0.9687384584349417 1107 68-69 0.9709756075018916 1107 70-71 1.1907088339920087 1107 72-73 1.4013590032352248 1107 74-75 1.364697105503506 1107 76-77 1.3767404085178505 1107 78-79 1.6946440181891695 1107 80-81 1.827284193236089 1107 82-83 2.0550899162780283 1107 84-85 2.2180520764476945 1107 86-87 2.2271139144704044 1107 88-89 2.465002852889743 1107 90-91 2.8465405636914305 1107 92-93 2.9999412124029945 1107 94-95 3.2284929387220096 1107 96-97 3.5238857500184864 1107 98-99 3.8345803210360394 1107 100-101 3.7086024670460347 1108 1 0.11938115303238561 1108 2 0.024263180335211132 1108 3 0.0238709330843605 1108 4 0.06263887174759475 1108 5 -0.019838343737248465 1108 6 0.07934412711320249 1108 7 0.015889595095543996 1108 8 0.05829858598465165 1108 9 0.06009856341311348 1108 10-11 0.033945479595438144 1108 12-13 -0.05153552115199744 1108 14-15 0.09617055497622573 1108 16-17 0.10289451221908053 1108 18-19 0.024963329016955527 1108 20-21 -0.0333837944481985 1108 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-2.1691694815358282 2316 84-85 -2.0508673797165002 2316 86-87 -1.946377587541754 2316 88-89 -2.22506581279999 2316 90-91 -2.4547842281200403 2316 92-93 -2.8484941749981125 2316 94-95 -3.0269245672657625 2316 96-97 -3.1721406714409817 2316 98-99 -3.367572000802838 2316 100-101 -3.0451041963149947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4672.0 3 1361.0 4 1424.0 5 1555.0 6 1829.0 7 2308.0 8 2874.0 9 3649.0 10 4655.0 11 5990.0 12 7692.0 13 9740.0 14 11717.0 15 14494.0 16 18553.0 17 23854.0 18 23068.0 19 17318.0 20 17312.0 21 18413.0 22 20484.0 23 22815.0 24 26035.0 25 29486.0 26 33405.0 27 39095.0 28 44693.0 29 52277.0 30 62056.0 31 75048.0 32 92043.0 33 117279.0 34 156216.0 35 213117.0 36 288330.0 37 350026.0 38 204793.0 39 12498.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64236194024501 19.384207094205596 15.057055124035134 21.916375841514256 2 15.147467996747 20.338975541460975 41.240727868478 23.272828593314024 3 19.165267190417882 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22-23 22.203506108255283 26.300710432834546 29.95384808144242 21.541935377467752 24-25 21.88095266818638 26.666968672700715 30.02637546719636 21.425703191916547 26-27 21.536190254864003 26.810817490906153 30.252200346225884 21.400791908003963 28-29 21.35748662417121 27.042090784314883 30.269692547935385 21.33073004357852 30-31 21.628390870719898 26.658927568788492 30.046259817565048 21.666421742926563 32-33 21.455926153846153 26.833304615384616 30.011593846153843 21.699175384615383 34-35 21.998073047927146 26.836647733566625 29.849467331171383 21.315811887334842 36-37 21.857178724744987 26.566893781261076 29.99889232405183 21.577035169942103 38-39 21.558072266977156 27.065525803429235 29.81328442653755 21.56311750305606 40-41 21.796042363241614 26.869856132919423 29.758469276707256 21.57563222713171 42-43 21.504383463944308 26.802538503432764 29.652485308264954 22.04059272435797 44-45 21.733380712018963 26.937218976147914 29.424181634919012 21.905218676914114 46-47 21.931959442348287 27.24907850926339 28.988325363938692 21.83063668444963 48-49 21.875676025546905 27.22363337321089 29.03722148978003 21.86346911146217 50-51 21.688731658544715 27.373484654415204 28.934583137390103 22.003200549649986 52-53 22.04193474165753 27.591096280601768 28.44898546765936 21.917983510081342 54-55 21.75029087107767 27.386643948208217 28.675852483576364 22.187212697137753 56-57 21.789066512259588 27.510921651286246 28.66006663357709 22.03994520287707 58-59 22.08697171444496 27.175713967569244 28.602061896260462 22.135252421725333 60-61 22.07031932501795 27.306568455722147 28.89755819544772 21.725554023812187 62-63 21.83594984255666 27.41010373838123 28.81545415440768 21.938492264654435 64-65 21.666983705983725 27.300358127284436 28.763578654501543 22.269079512230295 66-67 21.808427787569812 27.350188646003247 28.387821635099353 22.453561931327588 68-69 21.70477855340192 27.19955391768935 28.660542385693873 22.43512514321486 70-71 21.918067791963686 27.260107407780186 28.559430000007385 22.262394800248746 72-73 22.01701368048742 27.131896258333903 28.435372843913793 22.415717217264884 74-75 21.7238405177452 27.592866238903486 28.267165381941666 22.41612786140965 76-77 21.826748534854552 27.288307798532998 28.333010575228762 22.551933091383688 78-79 22.09161575549052 27.327188475155012 28.24389724140867 22.3372985279458 80-81 21.979870850383854 27.515965291379025 28.158686543334465 22.345477314902652 82-83 22.117715960445548 27.395850891968408 28.184922110407467 22.301511037178575 84-85 22.115062590078356 27.299441929434426 28.081784073765025 22.503711406722193 86-87 21.954312566295645 27.221228582093172 28.22541614165314 22.599042709958045 88-89 22.06126585717858 27.11058234706917 28.138013321703585 22.69013847404867 90-91 22.249169651899862 27.1131205459931 28.06767321633598 22.570036585771053 92-93 22.460767114239793 26.95781764973537 27.992363457302467 22.589051778722364 94-95 22.283927995237025 26.916012246096425 27.994621720010958 22.805438038655588 96-97 22.22903674600692 26.868833242231467 28.04154934378351 22.860580667978105 98-99 22.31981199892405 26.718981065275443 27.763333047036447 23.19787388876406 100-101 23.04466923301148 25.897274094348283 27.711152413334116 23.34690425930612 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1520.0 1 1010.0 2 720.5 3 921.0 4 730.5 5 680.0 6 1251.5 7 2811.0 8 4649.0 9 5533.5 10 4764.0 11 3528.5 12 3247.0 13 3294.5 14 3402.0 15 3510.0 16 3474.5 17 3514.0 18 3735.5 19 3941.0 20 4265.0 21 4913.5 22 5658.0 23 6588.5 24 7757.5 25 9312.5 26 11860.0 27 14732.5 28 17089.0 29 19874.0 30 23333.0 31 27197.0 32 31266.0 33 35679.5 34 40174.5 35 44513.0 36 50621.0 37 56434.0 38 59387.5 39 60874.0 40 61844.5 41 64035.0 42 66325.5 43 69126.5 44 72840.5 45 75060.5 46 74865.5 47 76110.0 48 81389.0 49 81113.0 50 75024.0 51 70224.5 52 66043.0 53 62979.0 54 60827.0 55 56859.0 56 51347.0 57 46531.5 58 42552.5 59 38031.5 60 32183.5 61 26233.5 62 21094.5 63 16222.5 64 12780.0 65 10461.0 66 8112.5 67 6513.5 68 5224.5 69 3791.5 70 2629.0 71 1819.5 72 1361.0 73 1075.5 74 795.0 75 573.0 76 400.0 77 273.0 78 183.0 79 112.0 80 58.0 81 34.5 82 27.0 83 21.0 84 14.5 85 8.0 86 5.0 87 8.0 88 8.5 89 2.0 90 3.0 91 2.5 92 1.0 93 2.5 94 2.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4665446954837529 2 0.04025245869694229 3 0.05201326264384841 4 0.056392808883491276 5 0.08267008632134848 6 0.08173512701176179 7 0.04689559063347922 8 0.03985879161922158 9 0.04596063132389254 10-11 0.049971114678172245 12-13 0.04468121332130024 14-15 0.039342103579713156 16-17 0.03966195808036123 18-19 0.047633716404205544 20-21 0.05538403699683196 22-23 0.04662494451754623 24-25 0.06443837978440822 26-27 0.05560547472804986 28-29 0.04418912947414936 30-31 0.04573919359267464 32-33 0.04546854747674165 34-35 0.048568675713792225 36-37 0.04399229593528901 38-39 0.027286049324516504 40-41 0.024948651050549806 42-43 0.024825630088762082 44-45 0.0156482663393981 46-47 0.026941590631510887 48-49 0.02649871516907509 50-51 0.01650941307191215 52-53 0.016066537609476354 54-55 0.017026101111420576 56-57 0.01552524537761038 58-59 0.015082369915174587 60-61 0.01756739334328655 62-63 0.016337183725409342 64-65 0.01744437238149883 66-67 0.0095218224423696 68-69 0.012892596795353154 70-71 0.018920623922951477 72-73 0.016657038226057415 74-75 0.014098202220872819 76-77 0.010259948213095926 78-79 0.01404899383615773 80-81 0.016952288534347947 82-83 0.014344244144448261 84-85 0.012917200987710698 86-87 0.0041089001237098795 88-89 0.029623447598483198 90-91 0.031813220718304634 92-93 0.017936456228649714 94-95 0.03294501356675167 96-97 0.027827341556382477 98-99 0.023792254009745228 100-101 0.14558300617958897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2032174.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.23007028958478 #Duplication Level Percentage of deduplicated Percentage of total 1 74.56447497820898 36.70814344283213 2 13.573451839148271 13.364439762271266 3 4.645945099246189 6.861606113923253 4 2.225964581944979 4.383375713251101 5 1.2194595582224166 3.0017039883297785 6 0.7625910352080807 2.2525446159300624 7 0.5077428354384576 1.749735083436784 8 0.37059276020689047 1.4595446107037164 9 0.2882036802701667 1.2769458693675593 >10 1.6225573147851182 14.980097996230818 >50 0.144692918148957 4.913348711868986 >100 0.07040210177307346 5.853705984208781 >500 0.002815290439937352 0.9594868592814684 >1k 8.043686971249576E-4 0.7787168422521697 >5k 1.005460871406197E-4 0.323871364025931 >10k+ 2.010921742812394E-4 1.1327330420861081 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12502 0.6152032257080349 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10382 0.5108814501120474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6543 0.32197046119082323 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3745 0.18428540075800595 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2641 0.12995934403254839 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2129 0.10476465105842316 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.214377312178977E-4 0.0 22-23 0.0 0.0 0.0 3.9366707772070704E-4 0.0 24-25 0.0 0.0 0.0 7.87334155441414E-4 0.0 26-27 0.0 0.0 0.0 0.0018207102344582698 0.0 28-29 0.0 0.0 0.0 0.0053637139339446326 0.0 30-31 0.0 0.0 0.0 0.016361787917766886 0.0 32-33 0.0 0.0 0.0 0.03759520592232751 0.0 34-35 0.0 0.0 0.0 0.06795677929153704 0.0 36-37 0.0 0.0 0.0 0.11578732923460294 0.0 38-39 0.0 0.0 0.0 0.1898705524231685 0.0 40-41 0.0 0.0 0.0 0.2660451319621253 0.0 42-43 0.0 0.0 0.0 0.34103871026792 0.0 44-45 0.0 0.0 0.0 0.4340179531870795 0.0 46-47 0.0 0.0 0.0 0.5441217139870898 0.0 48-49 0.0 0.0 0.0 0.6602781061070557 0.0 50-51 0.0 0.0 0.0 0.7790425426169216 0.0 52-53 0.0 0.0 0.0 0.8941655586578708 0.0 54-55 0.0 0.0 0.0 1.0195731271042736 0.0 56-57 0.0 0.0 0.0 1.1657466339004436 0.0 58-59 0.0 0.0 0.0 1.3256000716474081 0.0 60-61 0.0 0.0 0.0 1.4892671592097921 0.0 62-63 0.0 0.0 0.0 1.6549763947378522 0.0 64-65 0.0 0.0 0.0 1.8245976968507618 0.0 66-67 0.0 0.0 0.0 2.012795164193617 0.0 68-69 0.0 0.0 0.0 2.213663790600608 0.0 70-71 2.4604192357544186E-5 0.0 0.0 2.4298854330387063 0.0 72-73 4.920838471508837E-5 0.0 0.0 2.6468698054398887 0.0 74-75 4.920838471508837E-5 0.0 0.0 2.870103642699887 0.0 76-77 4.920838471508837E-5 0.0 0.0 3.107263452834255 0.0 78-79 4.920838471508837E-5 0.0 0.0 3.3652138055107486 0.0 80-81 4.920838471508837E-5 0.0 0.0 3.643339595920428 0.0 82-83 4.920838471508837E-5 0.0 0.0 3.918906550324923 0.0 84-85 4.920838471508837E-5 0.0 0.0 4.192972648995608 0.0 86-87 4.920838471508837E-5 0.0 0.0 4.471295272944147 0.0 88-89 4.920838471508837E-5 0.0 0.0 4.777322217487282 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9910 0.0 31.414865 1 GTATCAA 14070 0.0 29.999876 1 GTACATG 10960 0.0 24.919926 1 ATCAACG 16975 0.0 24.8615 3 TATCAAC 17145 0.0 24.587269 2 CAACGCA 17220 0.0 24.535378 5 TCAACGC 17345 0.0 24.35856 4 TACATGG 11130 0.0 24.16826 2 GAGTACT 8820 0.0 24.100683 12-13 AACGCAG 17625 0.0 24.048925 6 GGACCGA 610 0.0 23.369606 6 ACATGGG 11465 0.0 22.75739 3 GTACTTT 9340 0.0 22.607708 14-15 ACGCAGA 19370 0.0 21.826363 7 CGCAGAG 19515 0.0 21.56682 8 AGTACTT 9215 0.0 20.979929 12-13 GCAGAGT 20120 0.0 20.445616 9 AGAGTAC 15760 0.0 20.419857 10-11 AGGACCG 795 0.0 20.325253 5 CATGGGA 5535 0.0 20.177826 4 >>END_MODULE