FastQCFastQC Report
Thu 26 May 2016
SRR938278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032174
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123280.6066409667676095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105000.5166880395084279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65930.32443088042657764No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38210.18802523799635268No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.13089430334213506No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22460.11052203207008848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101650.030.14191
GTATCAA145450.029.2952351
GTACATG109700.024.9421631
ATCAACG169600.024.7298243
GTACCGT1351.3440791E-524.6279966
TATCAAC172650.024.459832
TCAACGC172050.024.4316844
CAACGCA171400.024.3857825
TACATGG109700.024.2926272
GAGTACT90650.024.28548212-13
AACGCAG174650.023.9319956
GTACTTT93650.023.4060814-15
ACATGGG113200.022.6166633
AGTACTT92800.021.70114712-13
ACGCAGA196250.021.2495487
CGCAGAG197600.021.1284088
GCAGAGT207300.020.0481159
AGAGTAC158500.020.03258510-11
GTATAGG4550.019.8362881
TGTACGC1200.00476317319.7927882
Produced by FastQC (version 0.11.5)