FastQCFastQC Report
Thu 26 May 2016
SRR938277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057854
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128320.6235622157840158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102910.5000840681603262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66990.32553329828063604No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39490.19189893938053915No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27500.1336343589000969No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21510.10452636581603943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113200.028.9842641
GTATCAA156000.027.461221
GTACATG111850.024.6799261
GAGTACT87900.024.0898812-13
TACATGG115000.023.800982
ATCAACG184400.023.0123353
TATCAAC184900.023.0015072
CAACGCA186400.022.816415
TCAACGC187550.022.7018414
TAGGACG1050.00219162322.6282444
GTACTTT93100.022.60463314-15
AACGCAG190150.022.4164226
ACATGGG116850.022.3668163
AGTACTT92350.020.71594812-13
ACGCAGA209400.020.3651077
CGCAGAG209700.020.2226188
GTATAGG4650.019.5142841
CATGGGA60250.019.4809474
AGAGTAC161900.019.46683510-11
GCAGAGT216550.019.1653359
Produced by FastQC (version 0.11.5)