##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938277_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2057854 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0095045615481 33.0 31.0 34.0 30.0 34.0 2 32.18393335970384 34.0 31.0 34.0 30.0 34.0 3 32.25462787933449 34.0 31.0 34.0 30.0 34.0 4 35.76602324557525 37.0 35.0 37.0 35.0 37.0 5 35.79753519929013 37.0 35.0 37.0 35.0 37.0 6 35.83323987027262 37.0 36.0 37.0 35.0 37.0 7 35.78141063457369 37.0 36.0 37.0 35.0 37.0 8 35.771384656054316 37.0 36.0 37.0 35.0 37.0 9 37.44486392134719 39.0 38.0 39.0 35.0 39.0 10-11 37.369082792073684 39.0 37.0 39.0 35.0 39.0 12-13 37.27762489467183 39.0 37.0 39.0 34.0 39.0 14-15 38.53448179511277 40.0 38.0 41.0 34.0 41.0 16-17 38.47572762693564 40.0 38.0 41.0 34.0 41.0 18-19 38.43836078749999 40.0 38.0 41.0 34.0 41.0 20-21 38.35404066566433 40.0 38.0 41.0 34.0 41.0 22-23 38.2068815377573 40.0 38.0 41.0 34.0 41.0 24-25 38.167983977483345 40.0 38.0 41.0 33.5 41.0 26-27 38.18868296779071 40.0 38.0 41.0 34.0 41.0 28-29 38.10098772799236 40.0 38.0 41.0 33.5 41.0 30-31 37.920079364230894 40.0 38.0 41.0 33.0 41.0 32-33 37.70597598274708 40.0 37.0 41.0 33.0 41.0 34-35 37.51234684287613 40.0 37.0 41.0 33.0 41.0 36-37 37.31299134924052 40.0 37.0 41.0 32.0 41.0 38-39 37.10149651044243 40.0 36.0 41.0 31.0 41.0 40-41 36.89619064326235 40.0 36.0 41.0 31.0 41.0 42-43 36.62059188844301 39.5 36.0 41.0 30.0 41.0 44-45 36.375141773906215 39.0 35.5 41.0 30.0 41.0 46-47 36.19199709989144 39.0 36.0 41.0 29.5 41.0 48-49 35.93498591250886 39.0 35.0 41.0 28.0 41.0 50-51 35.29616824128436 38.5 34.5 40.5 27.0 40.5 52-53 35.38186455404514 39.0 35.0 40.0 27.5 41.0 54-55 35.54042536545353 39.0 35.0 41.0 27.5 41.0 56-57 35.363633425889304 39.0 35.0 41.0 27.0 41.0 58-59 35.1356952436859 39.0 35.0 40.5 26.0 41.0 60-61 34.783241911233745 38.0 34.5 40.0 26.0 41.0 62-63 34.3969737406055 38.0 34.0 40.0 25.0 41.0 64-65 33.99055836808637 37.0 34.0 40.0 23.5 41.0 66-67 33.57527234682344 37.0 33.5 39.5 22.5 41.0 68-69 33.109081596653596 36.0 33.0 39.0 21.5 41.0 70-71 32.65230332181 35.5 32.5 39.0 20.0 40.0 72-73 32.16618453009786 35.0 32.0 38.0 18.5 40.0 74-75 31.645696925049105 35.0 31.5 37.0 15.0 39.5 76-77 31.12343684245821 35.0 31.0 37.0 9.5 39.0 78-79 30.566993090860674 35.0 30.5 36.0 7.0 39.0 80-81 30.04308590405345 34.0 30.0 36.0 2.0 37.5 82-83 29.527187545860883 34.0 29.5 35.0 2.0 37.0 84-85 29.04129860524605 34.0 29.0 35.0 2.0 36.5 86-87 28.561492214705222 34.0 29.0 35.0 2.0 36.0 88-89 28.04772738979539 34.0 27.5 35.0 2.0 36.0 90-91 27.47536171176381 33.0 26.5 35.0 2.0 35.0 92-93 26.8802111325682 33.0 25.5 35.0 2.0 35.0 94-95 26.311158371779534 33.0 24.5 35.0 2.0 35.0 96-97 25.509194043892325 32.0 21.5 34.5 2.0 35.0 98-99 24.50384939845101 32.0 10.0 34.0 2.0 35.0 100-101 21.60416579601857 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19935611844653067 1101 2 0.1279949771380302 1101 3 0.09317478794511658 1101 4 0.15157769049906022 1101 5 0.0611364522135176 1101 6 0.16485545107663313 1101 7 0.2462716497112254 1101 8 0.19292480205373863 1101 9 0.13942585923209805 1101 10-11 0.11215725007224364 1101 12-13 0.12152105924987922 1101 14-15 0.2218698549494178 1101 16-17 0.31300843438422987 1101 18-19 0.2511404584400694 1101 20-21 0.2839755432726818 1101 22-23 0.24881214105497662 1101 24-25 0.2509559909387775 1101 26-27 0.2898991133873636 1101 28-29 0.2589064622137798 1101 30-31 0.23450308326574287 1101 32-33 0.18692745575766168 1101 34-35 0.3030463651535982 1101 36-37 0.13756212264250678 1101 38-39 0.39339481999016357 1101 40-41 0.43051173202552206 1101 42-43 0.5089778617617071 1101 44-45 0.4422087137996513 1101 46-47 0.5675893297130941 1101 48-49 0.4391516591757991 1101 50-51 0.5828113833949615 1101 52-53 0.608419571567758 1101 54-55 0.6097190232616612 1101 56-57 0.47493197321100666 1101 58-59 0.5216556500922778 1101 60-61 0.3188801285314753 1101 62-63 0.35542044144675344 1101 64-65 0.1248593729091283 1101 66-67 0.06603211804625175 1101 68-69 0.20030425625702009 1101 70-71 0.24962859455624198 1101 72-73 0.2666529272069411 1101 74-75 0.2606929035580343 1101 76-77 0.20539994998847888 1101 78-79 0.2988405555080611 1101 80-81 0.4464195680177845 1101 82-83 0.41007530844489537 1101 84-85 0.38431743193897105 1101 86-87 0.5181853426857899 1101 88-89 0.47212024796397856 1101 90-91 0.5198256134494095 1101 92-93 0.4851585474848541 1101 94-95 0.8859224635715428 1101 96-97 0.8672823157427167 1101 98-99 0.9702119949709811 1101 100-101 0.9120045407079402 1102 1 0.39314651469424433 1102 2 0.27553827721693835 1102 3 0.2236063860507329 1102 4 0.20917408525703252 1102 5 0.15355290962929047 1102 6 0.23107489434257644 1102 7 0.130949992969299 1102 8 0.18424163853953957 1102 9 0.2262419473897168 1102 10-11 0.2670060781060144 1102 12-13 0.11936313781949792 1102 14-15 0.2350998204143835 1102 16-17 0.273761281555295 1102 18-19 0.1787403979104809 1102 20-21 0.3866098243041165 1102 22-23 0.30584746242150374 1102 24-25 0.2446002109845722 1102 26-27 0.18181025926230632 1102 28-29 0.38170120885320813 1102 30-31 0.21752059400699153 1102 32-33 0.2517101849236738 1102 34-35 0.25333764654296687 1102 36-37 0.3496853501135462 1102 38-39 0.22957365453293477 1102 40-41 0.2341011506788604 1102 42-43 0.21652509350022342 1102 44-45 0.1321315385692472 1102 46-47 0.3003230216174728 1102 48-49 0.17124015122887926 1102 50-51 0.2201841917883982 1102 52-53 0.2266796083553757 1102 54-55 0.2740373467301396 1102 56-57 0.21655543077741157 1102 58-59 0.28939285907000567 1102 60-61 0.26927488373902975 1102 62-63 0.2541140456246751 1102 64-65 0.2733634766220234 1102 66-67 0.29408996802960274 1102 68-69 0.30620720437983806 1102 70-71 0.3026255003608682 1102 72-73 0.3937631712185876 1102 74-75 0.36813707606460966 1102 76-77 0.18936876342881348 1102 78-79 0.2524842849601292 1102 80-81 0.3533015961184418 1102 82-83 0.18457372818030748 1102 84-85 0.35176912918597836 1102 86-87 0.34846236921792695 1102 88-89 0.33788326631771426 1102 90-91 0.37780166556566286 1102 92-93 0.26367465346968544 1102 94-95 0.47413553464683034 1102 96-97 0.748580571854756 1102 98-99 0.808708539394587 1102 100-101 0.545593385850303 1103 1 0.3315982292924602 1103 2 0.2803272497447651 1103 3 0.2611885773908824 1103 4 0.16626345024448597 1103 5 0.12783361476986954 1103 6 0.17510339591884616 1103 7 0.152797770103696 1103 8 0.26372565063501696 1103 9 0.2280150928571345 1103 10-11 0.22455449218308843 1103 12-13 0.06967340610747641 1103 14-15 0.16707868549834615 1103 16-17 0.09824046832747513 1103 18-19 0.14599527148700986 1103 20-21 0.12314685061821962 1103 22-23 0.2549232521660869 1103 24-25 0.33896712856805067 1103 26-27 0.18566151062947966 1103 28-29 0.261861248742548 1103 30-31 0.30178231954260326 1103 32-33 0.33161255254896815 1103 34-35 0.25060603102472356 1103 36-37 0.3286924594232019 1103 38-39 0.34290489689283987 1103 40-41 0.3451311963585866 1103 42-43 0.31685176788740677 1103 44-45 0.35820645975562826 1103 46-47 0.5152086614553397 1103 48-49 0.4430763475873647 1103 50-51 0.3062004048746729 1103 52-53 0.36214669965048785 1103 54-55 0.3918643428055475 1103 56-57 0.28619755263290614 1103 58-59 0.4163221841681164 1103 60-61 0.4537999110825268 1103 62-63 0.3493849071057298 1103 64-65 0.31789702870334935 1103 66-67 0.28701395310326205 1103 68-69 0.3514034341939052 1103 70-71 0.5049846009206007 1103 72-73 0.505529882921607 1103 74-75 0.2752566797447251 1103 76-77 0.241151077010489 1103 78-79 0.20123050512355078 1103 80-81 0.27428419278575333 1103 82-83 0.21947931268780252 1103 84-85 0.38887457858442076 1103 86-87 0.278219816300215 1103 88-89 0.30786010476589354 1103 90-91 0.3904395089678374 1103 92-93 0.4767072083175279 1103 94-95 0.6181082233169697 1103 96-97 0.47907162892224875 1103 98-99 0.5640067443743213 1103 100-101 0.5058039307901474 1104 1 0.21089405377936288 1104 2 0.317422083431957 1104 3 0.19643981589052828 1104 4 0.15855644387506374 1104 5 0.11577062821643125 1104 6 0.1608181871431924 1104 7 0.14499168376257643 1104 8 0.12768884950267534 1104 9 0.16081056348699718 1104 10-11 0.23301167548103763 1104 12-13 0.10421162266798234 1104 14-15 0.21669121203762387 1104 16-17 0.24883848672804731 1104 18-19 0.2582617258394535 1104 20-21 0.1758397027059715 1104 22-23 0.23395014530125735 1104 24-25 0.3179692517103021 1104 26-27 0.21440531813125574 1104 28-29 0.31897200599662057 1104 30-31 0.2198608758058711 1104 32-33 0.24601630343361336 1104 34-35 0.27980454482514716 1104 36-37 0.308959621489727 1104 38-39 0.24378423164159813 1104 40-41 0.22042135913282834 1104 42-43 0.3256256532377222 1104 44-45 0.39974396860205985 1104 46-47 0.2689825468056597 1104 48-49 0.33194754362416745 1104 50-51 0.1899052881018477 1104 52-53 0.2436647463596202 1104 54-55 0.18315238243754095 1104 56-57 0.21173010748075427 1104 58-59 0.23090994071447568 1104 60-61 0.32404230315612637 1104 62-63 0.27500769549921245 1104 64-65 0.39143913769131444 1104 66-67 0.343477151970923 1104 68-69 0.3564388198980808 1104 70-71 0.38540392151508485 1104 72-73 0.315038021633562 1104 74-75 0.374241110034891 1104 76-77 0.40617053914778367 1104 78-79 0.2914728971971492 1104 80-81 0.30948766058476096 1104 82-83 0.2270606998074065 1104 84-85 0.1466205092284376 1104 86-87 0.3756048127616438 1104 88-89 0.5494736928762975 1104 90-91 0.5193283978567287 1104 92-93 0.3700971587775399 1104 94-95 0.386001004633318 1104 96-97 0.4275518129279092 1104 98-99 0.7066288250537269 1104 100-101 0.6164532584617675 1105 1 0.31918557902844213 1105 2 0.32778877092216163 1105 3 0.2863003625835603 1105 4 0.18319637912887998 1105 5 0.19870037131407514 1105 6 0.16698689215522933 1105 7 0.1685195991116757 1105 8 0.18126974680398433 1105 9 0.23397846775883124 1105 10-11 0.28508420794536704 1105 12-13 0.16365638554798778 1105 14-15 0.2386737309988476 1105 16-17 0.26376569021266505 1105 18-19 0.2565673982143437 1105 20-21 0.3275660170040169 1105 22-23 0.25336219161109597 1105 24-25 0.26030012486059917 1105 26-27 0.21180801102269697 1105 28-29 0.25435731660009253 1105 30-31 0.26321811327363775 1105 32-33 0.26173753850560644 1105 34-35 0.2852597216212871 1105 36-37 0.3013413191018941 1105 38-39 0.400813839708249 1105 40-41 0.38794323994920177 1105 42-43 0.4785310735833974 1105 44-45 0.6471989886849627 1105 46-47 0.6645994602419876 1105 48-49 0.8282521705286072 1105 50-51 0.6018525787877209 1105 52-53 0.646614175215042 1105 54-55 0.7054869694767092 1105 56-57 0.8784756674514753 1105 58-59 0.7807994900888033 1105 60-61 0.7731732960954503 1105 62-63 0.9251556070270794 1105 64-65 0.7825588644521346 1105 66-67 0.863227580785555 1105 68-69 0.9029262967936162 1105 70-71 0.8502129460503625 1105 72-73 0.9332537341742793 1105 74-75 0.8823491412659017 1105 76-77 0.8565069359027468 1105 78-79 0.9243753764863172 1105 80-81 1.032785765642732 1105 82-83 1.046772594341551 1105 84-85 0.9777005531940155 1105 86-87 0.9896241528916114 1105 88-89 1.0881432190227187 1105 90-91 0.9241143769602012 1105 92-93 0.9461033435832249 1105 94-95 1.0344521734349321 1105 96-97 1.1063991763888232 1105 98-99 1.0533394036260901 1105 100-101 0.8720136263299736 1106 1 0.19926254262242793 1106 2 0.1711147770602821 1106 3 0.13208788819942185 1106 4 0.1459709641123652 1106 5 0.09983983430256416 1106 6 0.22897715621469672 1106 7 0.23013177581893984 1106 8 0.24589744421376736 1106 9 0.23976832869508513 1106 10-11 -0.2675492227269771 1106 12-13 -0.01704999278309316 1106 14-15 0.049303849391435506 1106 16-17 0.21215205010592797 1106 18-19 0.255958710720499 1106 20-21 0.1802514060056737 1106 22-23 0.10130835890671364 1106 24-25 0.2883645062785902 1106 26-27 0.2861019207824427 1106 28-29 0.20141705644132202 1106 30-31 0.30249840627986657 1106 32-33 0.3202379517757308 1106 34-35 0.1984413815037911 1106 36-37 0.09295157860289294 1106 38-39 0.30208753262994037 1106 40-41 0.44610278822276683 1106 42-43 0.35639917081226713 1106 44-45 0.28895994773680655 1106 46-47 0.5218312030589658 1106 48-49 0.39702659132580465 1106 50-51 0.38968502413841577 1106 52-53 0.4727039361608334 1106 54-55 0.20224339810896197 1106 56-57 0.328600897732926 1106 58-59 0.4072303199892673 1106 60-61 0.14330781157000416 1106 62-63 0.3463280908340849 1106 64-65 0.4923036516651891 1106 66-67 0.2241038001363833 1106 68-69 -0.17866128256520852 1106 70-71 0.04877305276124844 1106 72-73 0.18124512918148383 1106 74-75 0.17490704115000355 1106 76-77 0.3266852003925713 1106 78-79 0.46512569759287103 1106 80-81 0.34004356410866166 1106 82-83 0.3810001565271044 1106 84-85 0.2538283523279077 1106 86-87 0.22552860769372884 1106 88-89 0.24842994843709576 1106 90-91 -0.009289857312207772 1106 92-93 0.2481654365911865 1106 94-95 0.10642516734117535 1106 96-97 -0.07698274996495869 1106 98-99 0.1128720998962578 1106 100-101 -0.01810891170438822 1107 1 0.315848875287287 1107 2 0.3187897820445045 1107 3 0.17586557997486096 1107 4 0.19262003939177674 1107 5 0.16689046231508797 1107 6 0.16069771116432463 1107 7 0.06623446261015431 1107 8 0.19168430696576877 1107 9 0.18651638484679722 1107 10-11 0.24113071957327037 1107 12-13 0.22201836734778624 1107 14-15 0.240857755265786 1107 16-17 0.23891230295279797 1107 18-19 0.2744642637350694 1107 20-21 0.2830564113417253 1107 22-23 0.32532376896195814 1107 24-25 0.4204012370951844 1107 26-27 0.4628191333684981 1107 28-29 0.4373199050733021 1107 30-31 0.4675962730309706 1107 32-33 0.4530014414378556 1107 34-35 0.372873472885189 1107 36-37 0.3486213999916785 1107 38-39 0.27386742919257756 1107 40-41 0.46448520152654993 1107 42-43 0.6370649461819795 1107 44-45 0.5377348835282376 1107 46-47 0.3413133126181265 1107 48-49 0.2358153656752151 1107 50-51 0.11527017231653502 1107 52-53 0.15590638957297642 1107 54-55 0.28001679758590825 1107 56-57 0.2816303691703794 1107 58-59 0.21582476814744922 1107 60-61 0.14596399376986824 1107 62-63 0.14658128953466587 1107 64-65 0.07082984524081581 1107 66-67 0.13487571020920797 1107 68-69 0.18180395098976732 1107 70-71 0.22938382572680638 1107 72-73 0.23919913356760247 1107 74-75 0.2677889794863262 1107 76-77 0.38246244651953276 1107 78-79 0.11316202967135425 1107 80-81 0.3172751488581049 1107 82-83 0.34212143155085073 1107 84-85 0.335334526904834 1107 86-87 0.4207262762485371 1107 88-89 0.45576708151007495 1107 90-91 0.39343692498851013 1107 92-93 0.5355573375164937 1107 94-95 0.6208020217665826 1107 96-97 0.6599178821522216 1107 98-99 0.28796023274641414 1107 100-101 0.5431359721338183 1108 1 0.1740397017362767 1108 2 0.06352475743546648 1108 3 0.010006555387654714 1108 4 0.07365591074816535 1108 5 0.04748609925810854 1108 6 0.0946062375685699 1108 7 0.08565454052968136 1108 8 0.005200180068250404 1108 9 0.09898438218399974 1108 10-11 0.14656424243075605 1108 12-13 -0.05977247219691861 1108 14-15 0.07348060947769852 1108 16-17 0.15921132186520026 1108 18-19 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44-45 -0.5427107162355966 2316 46-47 -0.5481940183754119 2316 48-49 -0.46125682903072374 2316 50-51 -0.499829214307276 2316 52-53 -0.47184953918354466 2316 54-55 -0.49840285669296236 2316 56-57 -0.4938890325418228 2316 58-59 -0.6007460653225536 2316 60-61 -0.5060194745936997 2316 62-63 -0.5820545224507683 2316 64-65 -0.3863256636646213 2316 66-67 -0.3863597344065326 2316 68-69 -0.45812798721339476 2316 70-71 -0.6104044446835317 2316 72-73 -0.4737609805794136 2316 74-75 -0.5426785968576908 2316 76-77 -0.42537728844947154 2316 78-79 -0.45978070001671156 2316 80-81 -0.41924385924371066 2316 82-83 -0.3492814614855284 2316 84-85 -0.4802540846345096 2316 86-87 -0.431346082461733 2316 88-89 -0.4321610517926402 2316 90-91 -0.2835042302579893 2316 92-93 -0.447960555355877 2316 94-95 -0.6285690216289659 2316 96-97 -0.5683946772993487 2316 98-99 -0.37753063221241945 2316 100-101 -0.47325773666009496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4747.0 3 1259.0 4 1267.0 5 1403.0 6 1590.0 7 1900.0 8 2410.0 9 3100.0 10 3921.0 11 4983.0 12 6518.0 13 8414.0 14 9829.0 15 12298.0 16 15917.0 17 22289.0 18 19543.0 19 13616.0 20 13180.0 21 13864.0 22 14636.0 23 16074.0 24 17993.0 25 20074.0 26 23314.0 27 27186.0 28 31718.0 29 38210.0 30 45689.0 31 55949.0 32 70481.0 33 91794.0 34 128259.0 35 188530.0 36 285900.0 37 429168.0 38 365034.0 39 45797.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5767280313804 19.407046523621247 15.037838534748161 21.9783869102502 2 15.11230085359053 20.301715105834628 41.227834334301974 23.358149706272872 3 19.172312861168038 25.455451586161292 29.88872924765218 25.483506305018487 4 12.542967441267901 17.088654066936087 37.8396974664223 32.52868102537371 5 13.27254503072891 37.963979307530806 34.0279942632235 14.735481398516784 6 30.642086331810198 36.815239340600215 17.73113507460254 14.811539252987044 7 27.889078374649824 33.625488282437196 20.64978217242102 17.83565117049196 8 25.891150701062376 35.305062652937295 19.536932731170207 19.266853914830122 9 26.585275612487045 17.311051677371708 20.691660277350607 35.41201243279063 10-11 24.972834048802667 27.194375450587632 26.726822015301437 21.10596848530826 12-13 26.02656329200747 24.731112704517308 27.610651196141063 21.631672807334166 14-15 22.987756034768825 25.86188496045295 26.531370830603475 24.61898817417475 16-17 21.455496982514557 29.331981261273178 27.05873514809541 22.153786608116857 18-19 21.441258644012084 27.641632460155613 29.951483643598785 20.965625252233515 20-21 22.503291078175202 26.445825230253895 30.064236204826393 20.986647486744506 22-23 22.20108075936158 26.305277320829347 29.948184235773397 21.545457684035675 24-25 21.931543737431685 26.62302557838263 30.005420684251344 21.44000999993434 26-27 21.57636579451168 26.765288403420023 30.246290135994713 21.41205566607358 28-29 21.360562676213178 26.975970333456843 30.312313696657068 21.35115329367291 30-31 21.673969543102775 26.66394829947653 29.99786275066178 21.66421940675891 32-33 21.469369434865204 26.834414072653207 29.979033600460614 21.717182892020976 34-35 22.020830081216598 26.8052469590581 29.837775907152125 21.336147052573175 36-37 21.838297383992835 26.611532430752145 29.954136660255088 21.596033524999932 38-39 21.588927526058214 27.006741275888675 29.847950612266526 21.556380585786588 40-41 21.80359891373361 26.83900563648968 29.755697016073277 21.60169843370343 42-43 21.54062787585215 26.751690205002532 29.600378998725812 22.107302920419507 44-45 21.719332533140406 26.88954445627425 29.45049395590255 21.94062905468279 46-47 21.90163760714587 27.22412209592133 29.007259756170768 21.866980540762032 48-49 21.895585273096334 27.179632877330246 29.034932260598378 21.889849588975043 50-51 21.690643137134817 27.36931541593119 28.960309955211354 21.979731491722642 52-53 22.0055086134971 27.55337213617596 28.5033199688819 21.93779928144504 54-55 21.795113268474708 27.347410851793803 28.715678180607885 22.141797699123604 56-57 21.772098185696965 27.504214773738887 28.615792235289483 22.107894805274658 58-59 22.11283450102228 27.192008015897645 28.627938552885507 22.067218930194567 60-61 22.049800998217055 27.30823111038523 28.896186593093365 21.745781298304355 62-63 21.83201925479061 27.351197737795403 28.863619352386582 21.953163655027407 64-65 21.653067781213075 27.308655248690215 28.765762013104894 22.272514956991813 66-67 21.81222185851549 27.38196521683766 28.39473861352887 22.41107431111798 68-69 21.64268132025158 27.222199675669867 28.68540341871078 22.44971558536777 70-71 21.89002652909328 27.262107149923647 28.53608214344573 22.311784177537337 72-73 22.013202788146515 27.18233814139608 28.426780770957468 22.37767829949994 74-75 21.685910954770595 27.688903871566584 28.291400334582526 22.33378483908029 76-77 21.812731406379676 27.29680894627544 28.31258498317652 22.57787466416836 78-79 22.02874369885943 27.41683855277406 28.283202855415436 22.271214892951082 80-81 21.948979078540106 27.599058773669476 28.177544077761258 22.274418070029164 82-83 22.047617427482695 27.51851815848822 28.220482735827048 22.213381678202037 84-85 22.08214672018465 27.417136755428857 28.077719505915304 22.42299701847119 86-87 21.84132774347905 27.38537749133541 28.308417853028434 22.464876912157106 88-89 21.909621026686857 27.2954368122586 28.234593498800443 22.5603486622541 90-91 22.165166367530624 27.258088159682494 28.146987745024752 22.429757727762134 92-93 22.376477645372887 27.133282907778593 28.049279637247114 22.440959809601402 94-95 22.220274696716796 27.071420271196033 28.08275324895065 22.625551783136526 96-97 22.12340226827284 27.115819400619223 28.19645400363015 22.564324327477788 98-99 22.169700914539963 26.912198378877232 27.959105648117337 22.95899505846547 100-101 22.500885298208594 26.57936591960884 27.97797889157014 22.94176989061243 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1605.0 1 1065.5 2 695.0 3 897.5 4 759.5 5 701.0 6 1308.5 7 2971.5 8 4999.5 9 5795.5 10 4853.0 11 3734.0 12 3452.0 13 3485.5 14 3515.5 15 3475.0 16 3486.0 17 3558.5 18 3707.5 19 3856.5 20 4211.5 21 4841.0 22 5607.5 23 6624.0 24 7793.5 25 9390.0 26 11829.5 27 14700.5 28 17081.0 29 19897.5 30 23409.0 31 27334.0 32 31497.5 33 36125.0 34 40826.5 35 45351.0 36 51943.5 37 57512.5 38 60027.0 39 61713.0 40 63015.0 41 65485.5 42 67567.5 43 70207.5 44 74093.5 45 76128.5 46 75197.5 47 77088.0 48 83762.0 49 83017.0 50 76695.0 51 71430.0 52 66637.0 53 64136.0 54 61931.0 55 57526.0 56 51604.5 57 46797.0 58 42859.0 59 38211.5 60 32355.5 61 26221.5 62 20869.5 63 15880.0 64 12511.0 65 10187.0 66 7854.0 67 6340.5 68 5081.5 69 3561.5 70 2421.0 71 1729.0 72 1272.0 73 981.5 74 734.5 75 569.0 76 396.0 77 226.5 78 146.0 79 96.0 80 58.5 81 29.0 82 17.0 83 15.5 84 18.0 85 14.0 86 10.5 87 7.5 88 4.0 89 4.0 90 4.5 91 3.5 92 3.5 93 3.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4959535516125051 2 0.04922603838756297 3 0.056320807987349925 4 0.06584529320350228 5 0.08003483240307621 6 0.10821953355291483 7 0.06856657469383154 8 0.050878245006691435 9 0.05029511325876374 10-11 0.07345030308272599 12-13 0.10496371462698521 14-15 0.054741492836712424 16-17 0.06110734775158977 18-19 0.059673815537934174 20-21 0.07468945804707233 22-23 0.06497059558161075 24-25 0.08943783183841031 26-27 0.06691436807470307 28-29 0.06783766000892191 30-31 0.07155512490196098 32-33 0.07136074765265174 34-35 0.07294006280328925 36-37 0.06893103203628635 38-39 0.040090307670029066 40-41 0.04373488109457717 42-43 0.04154813703984831 44-45 0.021065634393888002 46-47 0.026896951873164956 48-49 0.027115626278637842 50-51 0.025487716815673026 52-53 0.025779282689636872 54-55 0.024661613506108793 56-57 0.024661613506108793 58-59 0.021502983204833775 60-61 0.028476267023802466 62-63 0.01904897043230472 64-65 0.022110412108925122 66-67 0.016303391785811822 68-69 0.018587324465195298 70-71 0.042131268787776004 72-73 0.03831661527008233 74-75 0.03224232622916883 76-77 0.022426275139052626 78-79 0.014092350574919308 80-81 0.01581744866253874 82-83 0.020118045303505496 84-85 0.017129495095376056 86-87 0.009597376684643322 88-89 0.04125657116588446 90-91 0.05333225777922049 92-93 0.033821641379806346 94-95 0.054085469620293765 96-97 0.04443949862332314 98-99 0.033991721472951916 100-101 0.1682092121209765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2057854.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.42304738013352 #Duplication Level Percentage of deduplicated Percentage of total 1 74.91089231965302 37.023245804022906 2 13.501732405298869 13.3459352076194 3 4.582512969839756 6.794452668854001 4 2.1146261299522893 4.180450696476014 5 1.2037073491883417 2.974544267537517 6 0.7208827166708214 2.1376932396924824 7 0.5022459396123072 1.737576740895712 8 0.35554591132232216 1.4057729936876702 9 0.27648612748545487 1.2298308280796946 >10 1.610610457468848 14.998963571049886 >50 0.14426285620003032 4.90203034356596 >100 0.0726515898325821 6.066414422325018 >500 0.0026606959445313366 0.9026723969914144 >1k 8.868986481771118E-4 0.8479782617900891 >5k 9.854429424190131E-5 0.32626537107184 >10k+ 1.9708858848380262E-4 1.1261731863403726 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12832 0.6235622157840158 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10291 0.5000840681603262 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6699 0.32553329828063604 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3949 0.19189893938053915 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2750 0.1336343589000969 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2151 0.10452636581603943 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.429715616365398E-5 0.0 12-13 0.0 0.0 0.0 9.718862465461592E-5 0.0 14-15 0.0 0.0 0.0 9.718862465461592E-5 0.0 16-17 0.0 0.0 0.0 1.9437724930923184E-4 0.0 18-19 0.0 0.0 0.0 1.9437724930923184E-4 0.0 20-21 0.0 0.0 0.0 2.1867440547288582E-4 0.0 22-23 0.0 0.0 0.0 3.4016018629115573E-4 0.0 24-25 0.0 0.0 0.0 5.588345917640417E-4 0.0 26-27 0.0 0.0 0.0 0.0020166639615832806 0.0 28-29 0.0 0.0 0.0 0.004980917013549066 0.0 30-31 0.0 0.0 0.0 0.01489415672831989 0.0 32-33 0.0 0.0 0.0 0.03510939065648 0.0 34-35 0.0 0.0 0.0 0.06613685907746614 0.0 36-37 2.429715616365398E-5 0.0 0.0 0.11536289746502912 0.0 38-39 4.859431232730796E-5 0.0 0.0 0.18910476642171895 0.0 40-41 4.859431232730796E-5 0.0 0.0 0.2671958263317028 0.0 42-43 4.859431232730796E-5 0.0 0.0 0.3441692170581586 0.0 44-45 4.859431232730796E-5 0.0 0.0 0.4369843536033169 0.0 46-47 4.859431232730796E-5 0.0 0.0 0.5454468587178682 0.0 48-49 4.859431232730796E-5 0.0 0.0 0.6561447021994757 0.0 50-51 4.859431232730796E-5 0.0 0.0 0.7777276716424003 0.0 52-53 4.859431232730796E-5 0.0 0.0 0.89136547101981 0.0 54-55 4.859431232730796E-5 0.0 0.0 1.020626341810449 0.0 56-57 4.859431232730796E-5 0.0 0.0 1.1679399996306832 0.0 58-59 4.859431232730796E-5 0.0 0.0 1.3243407938561238 0.0 60-61 4.859431232730796E-5 0.0 0.0 1.4913594453250814 0.0 62-63 4.859431232730796E-5 0.0 0.0 1.6558754897091825 0.0 64-65 4.859431232730796E-5 0.0 0.0 1.8222867122740487 0.0 66-67 4.859431232730796E-5 0.0 0.0 2.0060704014959274 0.0 68-69 4.859431232730796E-5 0.0 0.0 2.211308479610312 0.0 70-71 4.859431232730796E-5 0.0 0.0 2.4317322803269814 0.0 72-73 4.859431232730796E-5 0.0 0.0 2.6510384118601222 0.0 74-75 4.859431232730796E-5 0.0 0.0 2.8675746675906066 0.0 76-77 4.859431232730796E-5 0.0 0.0 3.1007058809808665 0.0 78-79 4.859431232730796E-5 0.0 0.0 3.360466777526491 0.0 80-81 4.859431232730796E-5 0.0 0.0 3.637916003759256 0.0 82-83 4.859431232730796E-5 0.0 0.0 3.9167258707371855 0.0 84-85 4.859431232730796E-5 0.0 0.0 4.19011747189062 0.0 86-87 4.859431232730796E-5 0.0 0.0 4.469413281991823 0.0 88-89 4.859431232730796E-5 0.0 0.0 4.781607441538613 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11320 0.0 28.984264 1 GTATCAA 15600 0.0 27.46122 1 GTACATG 11185 0.0 24.679926 1 GAGTACT 8790 0.0 24.08988 12-13 TACATGG 11500 0.0 23.80098 2 ATCAACG 18440 0.0 23.012335 3 TATCAAC 18490 0.0 23.001507 2 CAACGCA 18640 0.0 22.81641 5 TCAACGC 18755 0.0 22.701841 4 TAGGACG 105 0.002191623 22.628244 4 GTACTTT 9310 0.0 22.604633 14-15 AACGCAG 19015 0.0 22.416422 6 ACATGGG 11685 0.0 22.366816 3 AGTACTT 9235 0.0 20.715948 12-13 ACGCAGA 20940 0.0 20.365107 7 CGCAGAG 20970 0.0 20.222618 8 GTATAGG 465 0.0 19.514284 1 CATGGGA 6025 0.0 19.480947 4 AGAGTAC 16190 0.0 19.466835 10-11 GCAGAGT 21655 0.0 19.165335 9 >>END_MODULE