FastQCFastQC Report
Thu 26 May 2016
SRR938277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057854
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124520.6050963770996388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104690.5087338557545871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69140.33598107543100725No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39080.1899065725751195No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27580.13402311339871534No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22870.11113519229255331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA151700.026.8997331
GGTATCA111850.026.884881
GTACATG108450.025.5375581
TACATGG110150.024.9277862
ACATGGG112650.023.4451123
GAGTACT88300.022.59257112-13
ATCAACG180200.022.5382443
TATCAAC182500.022.3599412
CAACGCA182850.022.2354185
TCAACGC183050.022.2111234
AACGCAG186850.021.7594136
CATGGGG49550.021.5677764
GTACTTT92750.021.27816614-15
AGTACTT91100.020.90754512-13
GGACCGA5600.020.3558736
ATGGGGA23350.020.137935
AGAGTAC158250.019.91448210-11
ACGCAGA205950.019.6491767
CGCAGAG206200.019.5332138
GTACGTA1250.006028976819.0020471
Produced by FastQC (version 0.11.5)