##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938277_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2057854 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.546583965626326 34.0 31.0 34.0 31.0 34.0 2 32.757807405190064 34.0 31.0 34.0 31.0 34.0 3 32.81165622050933 34.0 31.0 34.0 31.0 34.0 4 36.25380906517178 37.0 35.0 37.0 35.0 37.0 5 36.22404650670067 37.0 37.0 37.0 35.0 37.0 6 36.26846025033846 37.0 37.0 37.0 35.0 37.0 7 36.23760383389686 37.0 37.0 37.0 35.0 37.0 8 36.23643416879915 37.0 37.0 37.0 35.0 37.0 9 37.9492719114184 39.0 38.0 39.0 35.0 39.0 10-11 37.939088973270216 39.0 38.0 39.0 35.0 39.0 12-13 37.88521051542043 39.0 38.0 39.0 35.0 39.0 14-15 39.19783351977351 40.5 39.0 41.0 36.0 41.0 16-17 39.161980393166864 40.0 39.0 41.0 36.0 41.0 18-19 39.20783131359173 40.0 39.0 41.0 36.0 41.0 20-21 39.153588884342625 40.0 39.0 41.0 36.0 41.0 22-23 39.01087710789978 40.0 38.5 41.0 35.0 41.0 24-25 38.97123897030596 40.0 38.5 41.0 35.0 41.0 26-27 38.93616918401402 40.0 38.0 41.0 35.0 41.0 28-29 38.88139075949994 40.0 38.0 41.0 35.0 41.0 30-31 38.69960721217346 40.0 38.0 41.0 35.0 41.0 32-33 38.53212059747679 40.0 38.0 41.0 35.0 41.0 34-35 38.33890766789092 40.0 38.0 41.0 34.0 41.0 36-37 38.209779216601376 40.0 38.0 41.0 34.0 41.0 38-39 38.2104814044145 40.0 38.0 41.0 34.0 41.0 40-41 38.16156685556896 40.0 38.0 41.0 34.0 41.0 42-43 38.00782003971128 40.0 38.0 41.0 33.5 41.0 44-45 37.784509250899234 40.0 38.0 41.0 33.0 41.0 46-47 37.43840257860859 40.0 37.0 41.0 33.0 41.0 48-49 37.1061302210944 40.0 37.0 41.0 32.0 41.0 50-51 36.83296847103828 40.0 36.5 41.0 31.0 41.0 52-53 36.460951797357836 39.5 36.0 41.0 30.0 41.0 54-55 36.26693657567544 39.0 35.5 41.0 30.0 41.0 56-57 36.052884704162686 39.0 35.0 41.0 30.0 41.0 58-59 35.751214128893494 39.0 35.0 40.5 28.5 41.0 60-61 35.41694478811422 38.0 35.0 40.0 28.0 41.0 62-63 35.08151015572533 38.0 34.5 40.0 28.0 41.0 64-65 34.71419158016069 37.5 34.0 40.0 27.0 41.0 66-67 34.30658491807485 37.0 34.0 39.5 26.0 41.0 68-69 33.865863175910434 36.0 34.0 39.0 26.0 41.0 70-71 33.37471122829899 35.5 33.0 39.0 25.5 40.0 72-73 32.96144721637201 35.0 33.0 38.0 24.5 40.0 74-75 32.51605677565075 35.0 32.5 37.0 23.5 39.5 76-77 31.175763683915378 34.0 30.5 36.0 22.0 38.5 78-79 31.886251648562045 35.0 32.0 36.0 23.5 39.0 80-81 31.707089278442496 35.0 32.0 36.0 23.0 37.5 82-83 31.34417067488753 35.0 32.0 35.5 21.0 37.0 84-85 30.961966446599224 35.0 31.0 35.0 20.0 37.0 86-87 30.546928499300726 34.0 31.0 35.0 18.0 36.0 88-89 30.110453657062163 34.0 31.0 35.0 14.0 36.0 90-91 29.786057465689986 34.0 30.0 35.0 7.5 35.5 92-93 29.39437637461161 34.0 30.0 35.0 2.0 35.0 94-95 29.013094466371278 34.0 29.5 35.0 2.0 35.0 96-97 28.47984720976318 34.0 29.0 35.0 2.0 35.0 98-99 27.841220028243015 34.0 29.0 35.0 2.0 35.0 100-101 25.449941055099146 31.5 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08804721824208173 1101 2 0.13049129900527845 1101 3 0.12910516734677202 1101 4 0.07494849781428314 1101 5 0.03936390632473774 1101 6 0.03241211045303061 1101 7 0.09255197867356912 1101 8 0.0767609027056011 1101 9 -0.01074257006356305 1101 10-11 -0.019825180345279136 1101 12-13 0.13696919575226474 1101 14-15 0.04728909796585157 1101 16-17 0.06809957412355772 1101 18-19 0.14309486101158342 1101 20-21 0.10353765984147145 1101 22-23 0.11329154640103667 1101 24-25 0.13218115310458955 1101 26-27 0.20484796711651398 1101 28-29 0.18019660781374824 1101 30-31 0.14685820822458595 1101 32-33 0.10113227032846339 1101 34-35 0.07745837864619176 1101 36-37 0.14311163610566524 1101 38-39 0.21184707019636306 1101 40-41 0.28185338142175453 1101 42-43 0.19953406082510838 1101 44-45 0.21654945246533686 1101 46-47 0.3379485627401664 1101 48-49 0.4922231167693667 1101 50-51 0.29719734377658114 1101 52-53 0.3079823114289084 1101 54-55 0.43396857285782886 1101 56-57 0.4284604429293637 1101 58-59 0.3887642269742244 1101 60-61 0.5122903503548955 1101 62-63 0.3841709628128456 1101 64-65 0.3168168993731584 1101 66-67 0.25856482354969046 1101 68-69 0.20301999322511222 1101 70-71 0.22134287388224294 1101 72-73 0.09419334415149194 1101 74-75 0.2886886238318098 1101 76-77 0.14137939770393615 1101 78-79 0.2962123501788021 1101 80-81 0.5119785909634409 1101 82-83 0.3635623273827342 1101 84-85 0.3878751472400417 1101 86-87 0.44829546853451774 1101 88-89 0.24488933533604396 1101 90-91 0.30029729316143516 1101 92-93 0.2490343876533494 1101 94-95 0.06915522898886195 1101 96-97 0.10842311072529753 1101 98-99 0.3185941350345054 1101 100-101 0.48272038476231316 1102 1 0.13651856964123965 1102 2 0.15454110186951908 1102 3 0.12865430480007944 1102 4 0.06416752787593794 1102 5 0.05312126004834283 1102 6 0.013703214952258236 1102 7 0.0757691817918058 1102 8 0.08319493581445414 1102 9 0.02791690675623215 1102 10-11 0.07859224029254364 1102 12-13 0.13140182064982042 1102 14-15 0.24271607343477797 1102 16-17 0.16134503129472222 1102 18-19 0.1459252758880467 1102 20-21 0.16488070508803077 1102 22-23 0.1829506735864257 1102 24-25 0.12219843444143663 1102 26-27 0.0745877313274903 1102 28-29 0.17219371123782423 1102 30-31 0.18807257280896295 1102 32-33 0.08617123452617648 1102 34-35 0.1283129100048015 1102 36-37 0.14515036398922376 1102 38-39 0.15835887968569295 1102 40-41 0.22766920424417236 1102 42-43 0.3073271767586263 1102 44-45 0.28385286603032256 1102 46-47 0.17361020093627388 1102 48-49 0.20299151092480372 1102 50-51 0.15448535546239128 1102 52-53 0.09569946825168785 1102 54-55 0.1987781119346579 1102 56-57 0.21903617194473668 1102 58-59 0.1398326502888949 1102 60-61 0.012117778828404369 1102 62-63 0.06828526147801739 1102 64-65 0.2105171576603908 1102 66-67 -0.054165876423915904 1102 68-69 0.1384338470392379 1102 70-71 0.18745977614906195 1102 72-73 0.19734585799521653 1102 74-75 0.16270415423272766 1102 76-77 0.19916684762537074 1102 78-79 0.1706804705691205 1102 80-81 0.18744634789981163 1102 82-83 0.22464846734981236 1102 84-85 0.22863395063356506 1102 86-87 0.2057162169179776 1102 88-89 -0.052355704484963184 1102 90-91 0.07402596442632259 1102 92-93 -0.020453066275752718 1102 94-95 0.05265331429136211 1102 96-97 0.22638913676407668 1102 98-99 0.11972177508519621 1102 100-101 0.3104663622392252 1103 1 0.06919147705936979 1103 2 0.09217122475441641 1103 3 0.1082168088211759 1103 4 0.022260881797315335 1103 5 -0.01876125941379314 1103 6 0.013158596796174038 1103 7 0.014926680344210297 1103 8 -0.05160690424216341 1103 9 -0.06169192036852422 1103 10-11 0.031404599553951584 1103 12-13 0.08355697087822023 1103 14-15 0.15977053525038798 1103 16-17 0.12463364996551007 1103 18-19 0.14577054385433286 1103 20-21 0.1455364665240495 1103 22-23 0.16650355912975812 1103 24-25 0.08779902634775993 1103 26-27 0.08675462317591354 1103 28-29 0.19435995970980713 1103 30-31 0.14797992660167125 1103 32-33 0.18292556123272874 1103 34-35 0.17714679020811275 1103 36-37 0.2376714345676163 1103 38-39 0.053367886955847155 1103 40-41 0.1450839568659319 1103 42-43 0.14302186246923299 1103 44-45 0.07237615625743388 1103 46-47 0.07246129145934077 1103 48-49 0.17002409794147155 1103 50-51 0.1933031512547032 1103 52-53 0.13510707939497024 1103 54-55 0.2529602196358738 1103 56-57 0.16441045618844896 1103 58-59 0.3014962609174745 1103 60-61 0.15280281389241424 1103 62-63 0.2913845666314572 1103 64-65 0.2500753188905307 1103 66-67 0.24117752575069318 1103 68-69 0.34236101682627407 1103 70-71 0.3861903944338181 1103 72-73 0.24612585542170962 1103 74-75 0.31066223825769157 1103 76-77 0.1423877509258915 1103 78-79 0.21877334195816545 1103 80-81 0.39255668695919255 1103 82-83 0.38128972064711775 1103 84-85 0.3426716203241007 1103 86-87 0.433618327192697 1103 88-89 0.3599118757762483 1103 90-91 0.3269851743600576 1103 92-93 0.27343308826326407 1103 94-95 0.14359039207557345 1103 96-97 0.20721056763327894 1103 98-99 0.16808631217584846 1103 100-101 0.5248080272974498 1104 1 0.0902919724592266 1104 2 0.03892635568152514 1104 3 -0.0226678337832098 1104 4 0.025130662405949522 1104 5 0.06055225792520247 1104 6 0.04646716013658647 1104 7 0.0042578905352925744 1104 8 0.03754737742804082 1104 9 0.15178295578151335 1104 10-11 0.0943469208561396 1104 12-13 0.1010746637303086 1104 14-15 0.1300447744577511 1104 16-17 0.09070229823161213 1104 18-19 0.11656318362786777 1104 20-21 0.17047808011216148 1104 22-23 0.14811183938453354 1104 24-25 0.12926753307105088 1104 26-27 0.14699170739388734 1104 28-29 0.056723724748728444 1104 30-31 0.050888631484895086 1104 32-33 0.14253101063117413 1104 34-35 0.14749357010902742 1104 36-37 0.1677811302505603 1104 38-39 0.07787248709738748 1104 40-41 0.259130312138403 1104 42-43 0.3516718978549349 1104 44-45 0.27108988591064787 1104 46-47 0.29969589867802426 1104 48-49 0.3122127037447271 1104 50-51 0.25017151643516655 1104 52-53 0.2274549208670109 1104 54-55 0.1780292217590116 1104 56-57 0.1793237612132188 1104 58-59 0.29752421562377407 1104 60-61 0.35326813590231865 1104 62-63 0.26489305920046746 1104 64-65 0.25382443424792456 1104 66-67 0.2544135483975438 1104 68-69 0.25921168915465387 1104 70-71 0.20613377730714433 1104 72-73 0.1508056147989265 1104 74-75 0.12455997357262305 1104 76-77 0.11998329232716998 1104 78-79 0.14513038371329756 1104 80-81 0.13115895164426306 1104 82-83 0.11586155362659767 1104 84-85 0.1847717618669158 1104 86-87 -0.02353489290638322 1104 88-89 0.03551343274723351 1104 90-91 -0.010007040785026788 1104 92-93 0.29632940815003295 1104 94-95 0.31886640056814386 1104 96-97 0.5074883454110548 1104 98-99 0.5446397445891513 1104 100-101 0.6412181476088428 1105 1 0.05562614732113502 1105 2 0.04396371997967208 1105 3 0.04627096004013964 1105 4 0.03888654183550955 1105 5 0.02251387821119266 1105 6 0.011895255598560084 1105 7 0.024052059515092594 1105 8 -0.058226946933991997 1105 9 0.004652310616762634 1105 10-11 0.03506289637452653 1105 12-13 0.0962597570820165 1105 14-15 0.13101618180695596 1105 16-17 0.10589811463240295 1105 18-19 0.040145894938483195 1105 20-21 0.042758407878949356 1105 22-23 0.03690716546612549 1105 24-25 0.056896880521456694 1105 26-27 0.12264721951340363 1105 28-29 -0.0018494549627021684 1105 30-31 0.17470389020126476 1105 32-33 0.18916446697365075 1105 34-35 0.1142690236650239 1105 36-37 0.08079187047369629 1105 38-39 0.09312465585821883 1105 40-41 0.06195930795008309 1105 42-43 0.05037300279054335 1105 44-45 0.05303872226124895 1105 46-47 0.03458380578357634 1105 48-49 0.08226850868183533 1105 50-51 0.11188926517449005 1105 52-53 0.10024161484042793 1105 54-55 0.11923170598571886 1105 56-57 0.07007639260008602 1105 58-59 0.07574494084107641 1105 60-61 -0.020764481917048272 1105 62-63 -0.02868368412688227 1105 64-65 -0.03839985243853761 1105 66-67 0.040648652590370205 1105 68-69 0.012287991323525205 1105 70-71 0.1778609932429447 1105 72-73 0.059959975183531355 1105 74-75 -0.05637376893250945 1105 76-77 0.04319917660840389 1105 78-79 0.06780126016959898 1105 80-81 0.165058653030421 1105 82-83 0.38966237540501325 1105 84-85 0.35630351561676576 1105 86-87 0.36593183129335927 1105 88-89 0.2790361314778558 1105 90-91 0.3872070595414989 1105 92-93 0.34476130981140685 1105 94-95 0.31690855857372213 1105 96-97 0.5330613147403476 1105 98-99 0.601508399581757 1105 100-101 0.6508514493848523 1106 1 -0.08411243956527414 1106 2 2.8847884351534958E-5 1106 3 0.039025348071476174 1106 4 -0.03198941236977504 1106 5 -0.04673066603675835 1106 6 -0.07105807827526434 1106 7 0.017788505861105364 1106 8 -0.10209017874207404 1106 9 -0.09060938548871178 1106 10-11 0.03381046608613758 1106 12-13 -0.09486671377466394 1106 14-15 -0.2317124871063143 1106 16-17 -0.12280037255928988 1106 18-19 -0.059404275443029064 1106 20-21 0.06311967378577776 1106 22-23 0.02411198071322218 1106 24-25 0.04212916586701709 1106 26-27 0.0031725085347957815 1106 28-29 -0.005638359335414123 1106 30-31 0.05088461646550968 1106 32-33 0.1489437995921179 1106 34-35 0.03307635820272736 1106 36-37 0.0781715186153562 1106 38-39 -0.046459815178963026 1106 40-41 0.03099209268707881 1106 42-43 -0.02426801818297264 1106 44-45 -0.06567267547900002 1106 46-47 0.0632618461744201 1106 48-49 0.023652859077792243 1106 50-51 0.02515136103786375 1106 52-53 -0.13578508735574246 1106 54-55 -0.29462046619367754 1106 56-57 -0.06200700893173661 1106 58-59 -0.0742328532193568 1106 60-61 -0.1058461187013009 1106 62-63 -0.07131462651023668 1106 64-65 -0.23188089223739183 1106 66-67 -0.1421587492535039 1106 68-69 -0.24597651889401106 1106 70-71 -0.11286995184286042 1106 72-73 -0.05065721736363571 1106 74-75 -0.2792919557244957 1106 76-77 -0.2741944477629623 1106 78-79 -0.14428305407477993 1106 80-81 -0.3002135265220609 1106 82-83 -0.20007269320394983 1106 84-85 -0.06419718115514073 1106 86-87 0.0937044761252892 1106 88-89 0.1861641870890729 1106 90-91 -0.08384605777979814 1106 92-93 -0.17210691846055326 1106 94-95 -0.26197440873128386 1106 96-97 -0.11688129654557144 1106 98-99 0.07708110121602374 1106 100-101 0.13002949309002076 1107 1 0.05948863468210419 1107 2 0.12078879581385138 1107 3 0.06056823000980671 1107 4 0.08715752262418874 1107 5 0.08896483877742156 1107 6 0.12423094821736669 1107 7 0.09713598266979062 1107 8 0.10840601565448083 1107 9 0.0885147979777301 1107 10-11 0.06346532306815789 1107 12-13 0.098524671136623 1107 14-15 0.10770981173617855 1107 16-17 0.05593855952623272 1107 18-19 0.03296692628327236 1107 20-21 0.09408297815195965 1107 22-23 0.06419735757936706 1107 24-25 0.039323574151374885 1107 26-27 -0.030558038322801906 1107 28-29 0.039889675472075226 1107 30-31 0.07565434520631698 1107 32-33 0.06369429508285407 1107 34-35 0.13046720364480535 1107 36-37 0.07225799787510567 1107 38-39 0.10485367506954191 1107 40-41 0.16640824627162232 1107 42-43 0.20262780562168814 1107 44-45 0.27558675057267834 1107 46-47 0.2038760201415144 1107 48-49 0.10972048037040594 1107 50-51 0.2155899729601316 1107 52-53 0.15251664011848476 1107 54-55 0.23871603358935545 1107 56-57 0.229287717170358 1107 58-59 0.2164122815893066 1107 60-61 0.1770211601456495 1107 62-63 0.2799310782593736 1107 64-65 0.25934534473032045 1107 66-67 0.29830284874811497 1107 68-69 0.35435068090896493 1107 70-71 0.4523402652348594 1107 72-73 0.2003442016749375 1107 74-75 0.25915707029903245 1107 76-77 0.33279472767008045 1107 78-79 0.4484180448005439 1107 80-81 0.5081122425147448 1107 82-83 0.5030597464869082 1107 84-85 0.6613085712284921 1107 86-87 0.6673069576836532 1107 88-89 0.6934648473524767 1107 90-91 0.5817267764272387 1107 92-93 0.6676126748265752 1107 94-95 0.6893655858740217 1107 96-97 0.8639612136281123 1107 98-99 0.8592232630802386 1107 100-101 0.9589100944809132 1108 1 0.03267471393303367 1108 2 0.013856140547730433 1108 3 0.0562839656072498 1108 4 0.03591201063614591 1108 5 -0.027765378502380145 1108 6 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98-99 -0.6353003043419392 2316 100-101 -0.6848515088254175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 27.0 6 54.0 7 158.0 8 394.0 9 741.0 10 1223.0 11 2223.0 12 3136.0 13 4868.0 14 6654.0 15 9132.0 16 12236.0 17 18311.0 18 17827.0 19 11471.0 20 9133.0 21 9240.0 22 9545.0 23 10196.0 24 11947.0 25 14033.0 26 16894.0 27 20105.0 28 25034.0 29 30687.0 30 37515.0 31 46697.0 32 59049.0 33 77287.0 34 109267.0 35 165954.0 36 272648.0 37 489508.0 38 493130.0 39 61517.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.910867340443005 19.747853832196064 15.327472211342496 22.013806616018435 2 15.264067265282316 20.509009611061835 40.742688424198974 23.48423469945687 3 19.273176136694982 25.282740120132964 30.073770969811246 25.37031277336081 4 12.721893778664569 17.411099135312806 37.44789474860705 32.41911233741558 5 13.267177004382724 38.024922091130904 34.06647607968111 14.641424824805272 6 30.83804779153429 36.956606250977956 17.445601097065193 14.759744860422558 7 27.95655085346191 33.57726058311231 20.537219841640855 17.928968721784926 8 26.031188968254625 35.31902093599949 19.352642548532614 19.29714754721327 9 26.398256819745676 17.18086078272059 20.667464263638518 35.75341813389522 10-11 25.01941342777476 27.19512657360532 26.652814048032564 21.13264595058736 12-13 25.976891460715873 24.64579605744625 27.661826349196783 21.71548613264109 14-15 22.84238823551136 25.789195929351642 26.49971766704538 24.868698168091612 16-17 21.45121991517352 29.273955122049834 27.116895902904393 22.157929059872256 18-19 21.37948076005392 27.593454151752262 29.990441498765218 21.0366235894286 20-21 22.33841662236485 26.473258063983156 30.093947384022385 21.094377929629605 22-23 22.107885204684102 26.330390785740875 30.01464146630422 21.547082543270804 24-25 21.86069126121494 26.590551020388325 30.053636357451307 21.495121360945426 26-27 21.505213545643617 26.765200646049024 30.3313261356771 21.398259672630257 28-29 21.303402771433515 26.968946260469657 30.33874642102542 21.388904547071412 30-31 21.622549510315114 26.563473404818804 30.106047367791884 21.707929717074194 32-33 21.40581002833799 26.742380415178353 30.045409297182456 21.806400259301203 34-35 21.975266518716822 26.740719355086433 29.891628196790794 21.392385929405954 36-37 21.791742314974282 26.524637374086872 30.089908094595696 21.593712216343146 38-39 21.553001021397836 26.950370466316837 29.89563175631095 21.600996755974375 40-41 21.749363196783737 26.81943949652571 29.824407071929965 21.606790234760588 42-43 21.488824895458542 26.72623709314851 29.66340918992854 22.121528821464402 44-45 21.680904059242327 26.83873027582321 29.53062892123596 21.949736743698505 46-47 21.90297363319926 27.20704540120213 29.048381978693328 21.841598986905282 48-49 21.851112858346607 27.098302406293158 29.06989514319286 21.980689592167373 50-51 21.602868813822553 27.326719971387668 29.011509076931603 22.058902137858176 52-53 22.010429311311686 27.476220373262635 28.46535760068499 22.047992714740698 54-55 21.69818655745257 27.296178446089957 28.72776688725245 22.277868109205027 56-57 21.76148064925889 27.45226337728527 28.617579284050276 22.168676689405565 58-59 22.108759902305994 27.106271873514835 28.646565791353517 22.13840243282565 60-61 21.959138014650215 27.317802915075607 28.91441764090164 21.808641429372543 62-63 21.764614982403998 27.384668688837984 28.8101585438034 22.04055778495462 64-65 21.522955467200298 27.343071957485808 28.762147363224017 22.371825212089878 66-67 21.69879398635666 27.30652903461567 28.44715903071841 22.54751794830926 68-69 21.53040980927887 27.274907397171074 28.665267955958836 22.529414837591222 70-71 21.770713568601078 27.272634501767374 28.55384784343301 22.402804086198536 72-73 21.807207897158882 27.190315736684916 28.392125972007733 22.61035039414847 74-75 21.502181398680374 27.677473717766176 28.233052490604287 22.58729239294916 76-77 21.553594181122666 27.2971503323365 28.375239448473994 22.77401603806684 78-79 21.696971699644386 27.394897791582885 28.373903104884988 22.534227403887737 80-81 21.644757111048694 27.561503391397057 28.22549607503739 22.568243422516854 82-83 21.64904393073752 27.50990959995685 28.342840815160265 22.498205654145366 84-85 21.663588116748723 27.32569017104297 28.21897990413974 22.791741808068572 86-87 21.40895663384437 27.3350960153053 28.401209223806024 22.854738127044307 88-89 21.48203041763197 27.33449068871554 28.226852993594047 22.956625900058437 90-91 21.62848395154838 27.236749679823056 28.291663153690035 22.84310321493853 92-93 21.793103079627922 27.125960834775782 28.267372777166027 22.813563308430272 94-95 21.49311855943133 27.04220027271128 28.272778340931865 23.191902826925524 96-97 21.336207524926454 27.076605045839013 28.45367066857027 23.133516760664268 98-99 21.331615362411522 26.977108191348854 28.219786243338934 23.47149020290069 100-101 21.262465655969763 26.921370515109427 28.32244172813037 23.493722100790436 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1447.0 1 979.0 2 685.5 3 871.0 4 703.0 5 655.0 6 1303.5 7 3049.5 8 4997.0 9 5736.5 10 4853.5 11 3599.5 12 3331.0 13 3427.0 14 3450.0 15 3419.5 16 3397.0 17 3508.0 18 3631.5 19 3840.0 20 4199.0 21 4832.0 22 5643.5 23 6547.5 24 7641.5 25 9192.5 26 11611.5 27 14565.5 28 16983.5 29 19697.0 30 23458.0 31 27503.5 32 31309.5 33 35773.0 34 40602.5 35 45115.5 36 51586.5 37 57324.0 38 60143.5 39 61844.0 40 62955.0 41 65031.0 42 67677.5 43 71123.0 44 74875.5 45 76676.0 46 75614.5 47 77747.0 48 85038.0 49 84040.5 50 76841.0 51 71748.0 52 66999.5 53 64383.0 54 62408.0 55 58211.0 56 52288.5 57 47012.5 58 43003.0 59 38443.0 60 32174.0 61 25529.5 62 20016.0 63 15266.0 64 12098.5 65 9711.0 66 7333.5 67 6003.0 68 4929.0 69 3389.0 70 2104.5 71 1418.0 72 1110.5 73 914.0 74 691.5 75 511.0 76 335.5 77 186.0 78 127.0 79 80.0 80 34.0 81 16.5 82 12.5 83 5.0 84 3.0 85 1.5 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015987528755684318 3 0.006511637851859267 4 0.0 5 4.859431232730796E-5 6 0.0 7 0.0 8 5.345374356003877E-4 9 2.915658739638478E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.915658739638478E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00524818573134926 26-27 0.020652582739105883 28-29 9.718862465461592E-5 30-31 0.0 32-33 7.532118410732735E-4 34-35 0.019437724930923186 36-37 0.004179110860148484 38-39 0.01844154152821337 40-41 0.015112831133792776 42-43 0.008625490438097164 44-45 0.0033287103944205955 46-47 4.859431232730796E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.158630301275017E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.859431232730796E-5 84-85 0.0032558189259296335 86-87 2.4297156163653982E-4 88-89 7.289146849096194E-5 90-91 3.6445734245480973E-4 92-93 3.887544986184637E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2057854.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.58307593159653 #Duplication Level Percentage of deduplicated Percentage of total 1 73.2577087309874 34.85827117121356 2 14.15184910116521 13.467770207064808 3 5.0281460771521225 7.177639697528661 4 2.2931091220827082 4.364527419019927 5 1.3005111891471048 3.09411613315388 6 0.8135733788086876 2.3227394315867604 7 0.5319794003504862 1.7719251340645663 8 0.39666695726830214 1.5099707157802382 9 0.2798570918887559 1.1984815128004618 >10 1.7039963010546018 15.311565515333667 >50 0.15866175102409258 5.20758129560196 >100 0.07975533203607282 6.421998381432887 >500 0.0029605230243706063 0.9967337166536938 >1k 9.187830075632913E-4 0.8474106772758753 >5k 1.020870008403657E-4 0.33585539826227584 >10k+ 2.041740016807314E-4 1.113413593226732 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12452 0.6050963770996388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10469 0.5087338557545871 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6914 0.33598107543100725 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3908 0.1899065725751195 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2758 0.13402311339871534 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2287 0.11113519229255331 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.429715616365398E-5 0.0 12-13 0.0 0.0 0.0 1.457829369819239E-4 0.0 14-15 0.0 0.0 0.0 1.457829369819239E-4 0.0 16-17 0.0 0.0 0.0 2.4297156163653982E-4 0.0 18-19 0.0 0.0 0.0 2.4297156163653982E-4 0.0 20-21 0.0 0.0 0.0 2.672687178001938E-4 0.0 22-23 0.0 0.0 0.0 3.8875449861846374E-4 0.0 24-25 0.0 0.0 0.0 6.074289040913494E-4 0.0 26-27 0.0 0.0 0.0 0.002113852586237896 0.0 28-29 0.0 0.0 0.0 0.0051024027943673365 0.0 30-31 0.0 0.0 0.0 0.015015642509138161 0.0 32-33 0.0 0.0 0.0 0.03527947074962558 0.0 34-35 0.0 0.0 0.0 0.06635553348293902 0.0 36-37 0.0 0.0 0.0 0.1159460292129568 0.0 38-39 0.0 0.0 0.0 0.19000376119977413 0.0 40-41 0.0 0.0 0.0 0.2682891983590673 0.0 42-43 0.0 0.0 0.0 0.34601580092659634 0.0 44-45 0.0 0.0 0.0 0.439292583438864 0.0 46-47 0.0 0.0 0.0 0.5481438430520338 0.0 48-49 0.0 0.0 0.0 0.6594977097500601 0.0 50-51 0.0 0.0 0.0 0.7818338910340579 0.0 52-53 0.0 0.0 0.0 0.8956903648169403 0.0 54-55 0.0 0.0 0.0 1.0257530417609801 0.0 56-57 0.0 0.0 0.0 1.1740142886715967 0.0 58-59 0.0 0.0 0.0 1.3316542378613838 0.0 60-61 0.0 0.0 0.0 1.4996204784207237 0.0 62-63 0.0 0.0 0.0 1.6646710602404253 0.0 64-65 0.0 0.0 0.0 1.8327830837367471 0.0 66-67 0.0 0.0 0.0 2.0170284189257353 0.0 68-69 0.0 0.0 0.0 2.222703845851066 0.0 70-71 0.0 0.0 0.0 2.4444396930005725 0.0 72-73 0.0 0.0 0.0 2.6650578709665504 0.0 74-75 0.0 0.0 0.0 2.8835378991901273 0.0 76-77 0.0 0.0 0.0 3.118831559478952 0.0 78-79 0.0 0.0 0.0 3.3810950631094334 0.0 80-81 0.0 0.0 0.0 3.661362759457182 0.0 82-83 0.0 0.0 0.0 3.943890091328151 0.0 84-85 0.0 0.0 0.0 4.220707591500661 0.0 86-87 0.0 0.0 0.0 4.50430399824283 0.0 88-89 0.0 0.0 0.0 4.821308994710023 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15170 0.0 26.899733 1 GGTATCA 11185 0.0 26.88488 1 GTACATG 10845 0.0 25.537558 1 TACATGG 11015 0.0 24.927786 2 ACATGGG 11265 0.0 23.445112 3 GAGTACT 8830 0.0 22.592571 12-13 ATCAACG 18020 0.0 22.538244 3 TATCAAC 18250 0.0 22.359941 2 CAACGCA 18285 0.0 22.235418 5 TCAACGC 18305 0.0 22.211123 4 AACGCAG 18685 0.0 21.759413 6 CATGGGG 4955 0.0 21.567776 4 GTACTTT 9275 0.0 21.278166 14-15 AGTACTT 9110 0.0 20.907545 12-13 GGACCGA 560 0.0 20.355873 6 ATGGGGA 2335 0.0 20.13793 5 AGAGTAC 15825 0.0 19.914482 10-11 ACGCAGA 20595 0.0 19.649176 7 CGCAGAG 20620 0.0 19.533213 8 GTACGTA 125 0.0060289768 19.002047 1 >>END_MODULE