FastQCFastQC Report
Thu 26 May 2016
SRR938276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996646
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119150.5967507510094427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97700.48932059063048733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62650.3137762026919143No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38180.19122067707545554No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25430.12736358873831416No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21100.10567722069911242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147200.030.3108061
GGTATCA102900.028.783311
GGACCGA5150.027.7013846
GTACATG109500.025.8742961
ATCAACG174100.025.4079573
TATCAAC175250.025.3226552
CAACGCA175700.025.1224385
TACATGG112850.025.036322
TCAACGC177000.024.9379224
GAGTACT85700.024.71350912-13
AACGCAG178900.024.6409156
ACATGGG114300.023.5535183
AGGACCG6750.023.2538975
GTACTTT92100.022.91586914-15
ACGCAGA196250.022.2663847
CGCAGAG197500.022.0745338
AGTACTT90650.021.4497912-13
CATGGGG54300.021.1107084
AGAGTAC154250.021.05000310-11
GCAGAGT203600.020.8760859
Produced by FastQC (version 0.11.5)