##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938276_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1996646 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08393075187089 33.0 31.0 34.0 30.0 34.0 2 32.193701337142386 34.0 31.0 34.0 30.0 34.0 3 32.21443610935539 34.0 31.0 34.0 30.0 34.0 4 35.72703674061401 37.0 35.0 37.0 35.0 37.0 5 35.74449601982525 37.0 35.0 37.0 35.0 37.0 6 35.78083946778748 37.0 36.0 37.0 35.0 37.0 7 35.72623038836128 37.0 36.0 37.0 35.0 37.0 8 35.72484256097476 37.0 36.0 37.0 35.0 37.0 9 37.373051106705944 39.0 37.0 39.0 35.0 39.0 10-11 37.28342505381525 39.0 37.0 39.0 34.0 39.0 12-13 37.13754791785825 39.0 37.0 39.0 34.0 39.0 14-15 38.33206912993089 40.0 38.0 41.0 33.5 41.0 16-17 38.17904851435858 40.0 38.0 41.0 33.0 41.0 18-19 38.375191446055034 40.0 38.0 41.0 34.0 41.0 20-21 38.3904582985667 40.0 38.0 41.0 34.0 41.0 22-23 38.2830990070348 40.0 38.0 41.0 34.0 41.0 24-25 38.17377091382248 40.0 38.0 41.0 33.5 41.0 26-27 38.007980883942366 40.0 38.0 41.0 33.0 41.0 28-29 37.838848498932705 40.0 38.0 41.0 33.0 41.0 30-31 37.63062756242218 40.0 37.5 41.0 32.5 41.0 32-33 37.346534137749 40.0 37.0 41.0 32.0 41.0 34-35 37.119629869290804 40.0 36.0 41.0 31.0 41.0 36-37 36.880670384234364 39.5 36.0 41.0 30.5 41.0 38-39 36.61088720784756 39.0 35.5 41.0 30.0 41.0 40-41 36.518261374324744 39.0 35.0 41.0 30.0 41.0 42-43 36.30286765906425 39.0 35.0 41.0 30.0 41.0 44-45 35.97119870022027 39.0 35.0 41.0 28.5 41.0 46-47 35.567870819364074 39.0 35.0 40.5 27.0 41.0 48-49 35.16605322125204 39.0 35.0 40.0 25.5 41.0 50-51 34.446732670688746 38.5 34.0 39.5 24.0 40.5 52-53 34.52295574678736 38.0 34.0 40.0 24.0 40.5 54-55 34.632664728750115 38.0 34.0 40.0 24.0 41.0 56-57 34.35522170680231 38.0 34.0 40.0 23.0 41.0 58-59 33.98178921050602 38.0 34.0 40.0 22.5 41.0 60-61 33.50092930845027 37.5 33.0 40.0 20.0 41.0 62-63 32.984820293632424 37.0 32.5 40.0 17.5 41.0 64-65 32.4832391420412 36.0 31.5 39.0 13.5 41.0 66-67 31.93710803016659 35.5 31.0 39.0 8.0 40.0 68-69 31.288741719864213 35.0 30.5 38.5 6.0 40.0 70-71 30.72766529469921 35.0 30.0 38.0 2.0 40.0 72-73 30.04352424015073 34.5 29.0 37.0 2.0 39.0 74-75 29.375253299783736 34.0 28.5 36.5 2.0 39.0 76-77 28.651546894141475 34.0 27.0 36.0 2.0 38.5 78-79 27.917440798218614 33.5 26.0 35.5 2.0 37.5 80-81 27.190962744522565 33.0 25.5 35.0 2.0 37.0 82-83 26.47634783531983 32.5 23.5 35.0 2.0 36.5 84-85 25.747702396919635 32.0 21.0 35.0 2.0 36.0 86-87 25.0130283485405 31.5 18.5 34.5 2.0 35.5 88-89 24.157600295695882 31.0 10.5 34.0 2.0 35.0 90-91 23.277965147552447 30.5 4.0 34.0 2.0 35.0 92-93 22.338077956733443 29.5 2.0 34.0 2.0 35.0 94-95 21.267529146378475 29.0 2.0 34.0 2.0 35.0 96-97 20.0001762956478 28.0 2.0 34.0 2.0 35.0 98-99 18.450084291356603 25.5 2.0 33.0 2.0 35.0 100-101 14.35664910054161 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2635911691484978 1101 2 0.2383219022278098 1101 3 0.02100973948694218 1101 4 -0.021282344233746642 1101 5 0.09365317819602836 1101 6 0.1260210080968065 1101 7 0.14439081471915927 1101 8 0.1584704900961995 1101 9 0.2114640373090353 1101 10-11 0.13179821463753427 1101 12-13 0.2584405813276476 1101 14-15 0.28885012932774856 1101 16-17 0.3016855536481131 1101 18-19 0.33747308383231456 1101 20-21 0.3349973113764264 1101 22-23 0.2566550211368508 1101 24-25 0.294276031740182 1101 26-27 0.4476414147921943 1101 28-29 0.47672358530340375 1101 30-31 0.24282235391229534 1101 32-33 0.2920141302944046 1101 34-35 0.27257084095902684 1101 36-37 0.3149591148429991 1101 38-39 0.10158873545762503 1101 40-41 0.38264245533388674 1101 42-43 0.5076062106060633 1101 44-45 0.5909652337135327 1101 46-47 0.3933595020181784 1101 48-49 0.26859096393560833 1101 50-51 0.42067387635950837 1101 52-53 0.5219045260138202 1101 54-55 0.40866341945306317 1101 56-57 0.34371918633483034 1101 58-59 0.424832155252723 1101 60-61 0.32006204431136354 1101 62-63 0.1893489578859473 1101 64-65 0.18528440434737092 1101 66-67 0.14738590966832987 1101 68-69 0.3260421205673154 1101 70-71 0.21035584373735006 1101 72-73 0.1714392601844672 1101 74-75 0.08249326326237494 1101 76-77 -0.10315406512365044 1101 78-79 -0.18613711337088645 1101 80-81 -0.2926886137923752 1101 82-83 -0.3307301687352222 1101 84-85 -0.5907183767682049 1101 86-87 -0.4436682918496615 1101 88-89 -0.456632086419674 1101 90-91 -0.3792420780299608 1101 92-93 -0.7940304464817132 1101 94-95 -0.8803166601545769 1101 96-97 -0.9783757898430494 1101 98-99 -1.240070868679524 1101 100-101 -1.3030544095487482 1102 1 0.22691496832678126 1102 2 0.16377408106694702 1102 3 0.20438636343241967 1102 4 0.21790120796173795 1102 5 0.18617171358629037 1102 6 0.23488704226792123 1102 7 0.22745294295281582 1102 8 0.29255093325190273 1102 9 0.40558228127243723 1102 10-11 0.450639778297365 1102 12-13 0.29567196782448235 1102 14-15 0.249008895640209 1102 16-17 0.44723034373609494 1102 18-19 0.34643069991393816 1102 20-21 0.38532885322304367 1102 22-23 0.4150172635467584 1102 24-25 0.31197809596505266 1102 26-27 0.33471588194534974 1102 28-29 0.2658537725115693 1102 30-31 0.3906822427145542 1102 32-33 0.411910875249184 1102 34-35 0.22009776436166106 1102 36-37 0.38302867475341174 1102 38-39 0.16796298973484625 1102 40-41 0.4196710767797498 1102 42-43 0.4942122193138303 1102 44-45 0.2214924520471513 1102 46-47 0.3391874696516908 1102 48-49 0.32158893007986933 1102 50-51 0.25244330895316835 1102 52-53 0.32080977724537973 1102 54-55 0.4158269937245649 1102 56-57 0.23901576717950945 1102 58-59 0.13869653956239603 1102 60-61 0.28515086671911405 1102 62-63 0.21509628671647363 1102 64-65 0.2538989295522853 1102 66-67 0.017176983187908945 1102 68-69 0.13680319580975464 1102 70-71 -0.050462976655520464 1102 72-73 -0.1042509440187338 1102 74-75 -0.08743918002181417 1102 76-77 -0.33602945240307136 1102 78-79 -0.4782985559630113 1102 80-81 -0.6202614851061021 1102 82-83 -0.6110392549455916 1102 84-85 -0.5476159291330553 1102 86-87 -0.51948490026016 1102 88-89 -0.6915541049156388 1102 90-91 -0.9904651874785628 1102 92-93 -1.1284360072447939 1102 94-95 -1.2135779033032748 1102 96-97 -1.176975123182224 1102 98-99 -1.200245148069687 1102 100-101 -1.1267982882707912 1103 1 0.11695726928143557 1103 2 0.13803466309549606 1103 3 0.19127069103056726 1103 4 0.0631302985251665 1103 5 0.11096767768140126 1103 6 0.0835827062503185 1103 7 0.08452290588006406 1103 8 0.050663482308785035 1103 9 0.1324861637260284 1103 10-11 0.10512381528863557 1103 12-13 0.03668420318889076 1103 14-15 0.1345213279111519 1103 16-17 0.16609738429884402 1103 18-19 0.1722555006309463 1103 20-21 0.12551594837642455 1103 22-23 0.1905142827892803 1103 24-25 0.21785982982890317 1103 26-27 0.095259377918957 1103 28-29 0.22653900723774 1103 30-31 0.2304588896000368 1103 32-33 0.09700029797355825 1103 34-35 0.15698701462306275 1103 36-37 0.0015843993015423052 1103 38-39 0.1125373660843394 1103 40-41 0.26893318653137754 1103 42-43 0.17562203612285998 1103 44-45 0.19335230212659127 1103 46-47 0.24456539439265867 1103 48-49 0.31980268230354625 1103 50-51 0.2595697976344411 1103 52-53 0.2100980619857964 1103 54-55 0.22583986054785044 1103 56-57 0.20042887708051182 1103 58-59 0.2346877941964962 1103 60-61 0.19808304260417486 1103 62-63 0.16142567739261438 1103 64-65 -0.08834838505003262 1103 66-67 -0.3161204203618375 1103 68-69 -0.34120149599757355 1103 70-71 -0.44540774097877645 1103 72-73 -0.49946255064022793 1103 74-75 -0.6903408353946432 1103 76-77 -0.5526222225384636 1103 78-79 -0.7652119102997084 1103 80-81 -0.41766588359059753 1103 82-83 -0.38955522474420334 1103 84-85 -0.6323684040444455 1103 86-87 -0.9196644925957429 1103 88-89 -1.1023398298711662 1103 90-91 -1.2649316726290927 1103 92-93 -1.291047361282736 1103 94-95 -1.2591660087908565 1103 96-97 -1.4450828853734556 1103 98-99 -1.6198015312362166 1103 100-101 -1.7173768200270612 1104 1 0.23859530099024795 1104 2 0.294380432765756 1104 3 0.3388117416304297 1104 4 0.26263990751692745 1104 5 0.2994783821940956 1104 6 0.3117940207654186 1104 7 0.3825592985512358 1104 8 0.3978945806431895 1104 9 0.27070129763264106 1104 10-11 0.3821540262039207 1104 12-13 0.3497466793176969 1104 14-15 0.416932322729032 1104 16-17 0.3296691912168157 1104 18-19 0.3822563336669589 1104 20-21 0.29583471824943786 1104 22-23 0.4040291131878604 1104 24-25 0.38572475854144983 1104 26-27 0.2889751428896332 1104 28-29 0.3345897501329631 1104 30-31 0.2764102560148487 1104 32-33 0.30998654344883647 1104 34-35 0.29128546470456484 1104 36-37 0.1800402020260492 1104 38-39 0.15416857632848036 1104 40-41 0.25498612046847313 1104 42-43 0.15563514465173256 1104 44-45 0.22473449747510443 1104 46-47 0.21564332726881474 1104 48-49 0.11358116073970592 1104 50-51 0.05726495989571134 1104 52-53 0.18737912676299118 1104 54-55 0.16581285760707942 1104 56-57 0.05357720307107883 1104 58-59 0.17014306409229363 1104 60-61 0.2961073326767334 1104 62-63 0.15244696382025325 1104 64-65 -0.09842576309277007 1104 66-67 0.20406734281073824 1104 68-69 0.19095573719121006 1104 70-71 0.07134209830814697 1104 72-73 -0.028046059356455544 1104 74-75 -0.052400131840723674 1104 76-77 -0.17399022483952464 1104 78-79 -0.417781512029908 1104 80-81 -0.4248975791960987 1104 82-83 -0.35203779778809974 1104 84-85 -0.27238100960818556 1104 86-87 -0.436585182852518 1104 88-89 -0.5095218576694194 1104 90-91 -0.8381131000127127 1104 92-93 -1.2306549227348604 1104 94-95 -1.2612525282249578 1104 96-97 -1.6032724937993805 1104 98-99 -1.736208568347184 1104 100-101 -1.861841138580031 1105 1 0.06393322223679831 1105 2 0.14288323509665446 1105 3 0.17460894524480608 1105 4 0.09843886835399474 1105 5 0.14772375139976646 1105 6 0.07707827057337369 1105 7 0.10793936385548619 1105 8 0.1858798047432586 1105 9 0.16434012217951732 1105 10-11 0.11137552676081697 1105 12-13 0.20668605425601072 1105 14-15 0.16391275517568005 1105 16-17 0.28100627661110167 1105 18-19 0.2584153318625866 1105 20-21 0.25831991730909465 1105 22-23 0.2974071365993467 1105 24-25 0.24269503131220205 1105 26-27 0.29137565553006795 1105 28-29 0.21283545028223472 1105 30-31 0.10796567047193406 1105 32-33 0.10532413375177896 1105 34-35 -0.05550126322669513 1105 36-37 0.03782538682290948 1105 38-39 0.22342072284922665 1105 40-41 0.1872223415727774 1105 42-43 0.17455545430951958 1105 44-45 0.18737565004918366 1105 46-47 0.29193358999996377 1105 48-49 0.33654153038907 1105 50-51 0.31803475815775784 1105 52-53 0.35783631803580107 1105 54-55 0.43154344198026706 1105 56-57 0.34690335912592474 1105 58-59 0.23109335841245837 1105 60-61 0.16658537632556403 1105 62-63 0.37576624620564303 1105 64-65 0.32096003485767 1105 66-67 0.16442425231289803 1105 68-69 0.2134804034726514 1105 70-71 -0.13896480356472907 1105 72-73 -0.3564983649644269 1105 74-75 -0.5539927613458389 1105 76-77 -0.6822613256429015 1105 78-79 -0.9195112049054508 1105 80-81 -0.8542989607831579 1105 82-83 -0.6459622881715532 1105 84-85 -0.525095699023467 1105 86-87 -0.5958889887918737 1105 88-89 -0.5028934672180512 1105 90-91 -0.5671237338458681 1105 92-93 -0.7728521364239782 1105 94-95 -0.9825898098424446 1105 96-97 -1.227966480788318 1105 98-99 -1.3056468340386687 1105 100-101 -1.5787940031312555 1106 1 0.15836850978510597 1106 2 0.09122201358575666 1106 3 0.15997682388722012 1106 4 0.1736873290974401 1106 5 0.14013982089529975 1106 6 0.07376862407582507 1106 7 0.1824754439311178 1106 8 0.13254125891823776 1106 9 0.3558382706632841 1106 10-11 0.20255290495504852 1106 12-13 0.18874612322253626 1106 14-15 0.1855509735566372 1106 16-17 -0.06562891101410884 1106 18-19 0.2310737822218627 1106 20-21 0.2981334860331657 1106 22-23 0.2426877600900923 1106 24-25 0.2831752132310257 1106 26-27 0.2991735757581253 1106 28-29 0.2185704667156685 1106 30-31 0.19341415497160597 1106 32-33 0.348997813811728 1106 34-35 0.27958812263810273 1106 36-37 0.19075808690177354 1106 38-39 0.046748480408354 1106 40-41 0.13838982542100098 1106 42-43 -0.051137578503464454 1106 44-45 0.22329959583600356 1106 46-47 0.19260500954472803 1106 48-49 0.08817660780498215 1106 50-51 -0.05450481922631667 1106 52-53 -0.019955038616181753 1106 54-55 0.07584889685078622 1106 56-57 -0.053241504875799706 1106 58-59 -0.0822437730598864 1106 60-61 0.08584328812873565 1106 62-63 -0.15095767215129996 1106 64-65 -0.3770755149887819 1106 66-67 -0.3217426437523905 1106 68-69 -0.3563826492269868 1106 70-71 -0.4552292164920395 1106 72-73 -0.42684649165124 1106 74-75 -0.2993994826436648 1106 76-77 -0.7035848352458025 1106 78-79 -0.6624152566891262 1106 80-81 -0.5647916820711849 1106 82-83 -0.6454537947081889 1106 84-85 -0.78073611914132 1106 86-87 -0.7566745606801746 1106 88-89 -1.039720057783157 1106 90-91 -0.8772184158797955 1106 92-93 -0.6940230979878699 1106 94-95 -0.6524094829371734 1106 96-97 -0.6315913564967168 1106 98-99 -1.0377788451869527 1106 100-101 -1.2082991143936663 1107 1 -0.011777151001489017 1107 2 -0.03385997800081242 1107 3 -0.07493235129928166 1107 4 0.043375367508858176 1107 5 0.008343811589803352 1107 6 0.08971380122137873 1107 7 0.1642574600058424 1107 8 0.050387329006831294 1107 9 -0.0032165521911622363 1107 10-11 0.1847399594674286 1107 12-13 0.06519518786252121 1107 14-15 0.061364603400697604 1107 16-17 0.11777642915258468 1107 18-19 0.20589881217612316 1107 20-21 0.14392398331760603 1107 22-23 0.10964575197401416 1107 24-25 0.15220677233930502 1107 26-27 0.049451253931309225 1107 28-29 0.10699112291073476 1107 30-31 0.02641888424631844 1107 32-33 0.0032278144239370476 1107 34-35 0.11091202438813497 1107 36-37 0.008078372276223433 1107 38-39 -0.022475376300960193 1107 40-41 -0.024836838680244 1107 42-43 -0.07748803459533349 1107 44-45 -0.07357444759548315 1107 46-47 0.05151279415392196 1107 48-49 8.910977954457167E-4 1107 50-51 0.04502476771100561 1107 52-53 0.03567024015520559 1107 54-55 0.0312435606196928 1107 56-57 -0.06954033906475843 1107 58-59 -0.014494944992968328 1107 60-61 0.021669566541554275 1107 62-63 -0.020563718976148948 1107 64-65 -0.24175140771317416 1107 66-67 -0.13060057747694742 1107 68-69 -0.132667785354883 1107 70-71 -0.34106331143610547 1107 72-73 -0.28319449892772397 1107 74-75 -0.2954442085075293 1107 76-77 -0.5762565474925019 1107 78-79 -0.8500114601507036 1107 80-81 -0.8681665650965229 1107 82-83 -0.6791889596971288 1107 84-85 -0.9269760396422733 1107 86-87 -0.8201972690699932 1107 88-89 -0.7428238766232909 1107 90-91 -1.0679286597992999 1107 92-93 -1.3574159317045584 1107 94-95 -1.3770084894177863 1107 96-97 -1.5584846515527317 1107 98-99 -1.561550939186482 1107 100-101 -1.6649583617327668 1108 1 -0.010248961110256971 1108 2 -0.01735419638602309 1108 3 -0.061447905344635956 1108 4 0.007834638919376857 1108 5 0.005806217896683563 1108 6 -0.09381759054928551 1108 7 -0.005755107082585198 1108 8 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42-43 -0.5404007880041632 2316 44-45 -0.5599521423464111 2316 46-47 -0.5853276161735153 2316 48-49 -0.7317972337495746 2316 50-51 -0.663020370302128 2316 52-53 -0.7151868648390831 2316 54-55 -0.5510928448971271 2316 56-57 -0.630861463461116 2316 58-59 -0.44622542786440533 2316 60-61 -0.6140811163637991 2316 62-63 -0.3362952292336274 2316 64-65 -0.20331598412661123 2316 66-67 -0.16025281229094546 2316 68-69 -0.20308790217195138 2316 70-71 -0.32721782402621713 2316 72-73 -0.09646583998711122 2316 74-75 0.04792501921451375 2316 76-77 -0.21758500323582197 2316 78-79 -3.486023645855596E-4 2316 80-81 -0.03898035885839235 2316 82-83 -0.026776561812319244 2316 84-85 -0.14327357405828067 2316 86-87 -0.007812778751784322 2316 88-89 -0.0326349074149519 2316 90-91 -0.028219156407214285 2316 92-93 -0.04919513347833515 2316 94-95 -0.056523181323097305 2316 96-97 -0.3860058084378579 2316 98-99 -0.3549594241696852 2316 100-101 -0.2983349065523022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5368.0 3 1475.0 4 1421.0 5 1569.0 6 1940.0 7 2329.0 8 3017.0 9 3786.0 10 4738.0 11 6340.0 12 8094.0 13 10433.0 14 12432.0 15 15393.0 16 19118.0 17 24730.0 18 24275.0 19 18821.0 20 18945.0 21 20504.0 22 22383.0 23 24968.0 24 28155.0 25 32329.0 26 36239.0 27 41772.0 28 48385.0 29 56194.0 30 66445.0 31 79614.0 32 97098.0 33 121731.0 34 157195.0 35 209402.0 36 271744.0 37 311916.0 38 175211.0 39 11137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61943044072955 19.388007425326663 15.03508271234082 21.957479421602965 2 15.113603450746782 20.336375653554324 41.187777669763776 23.36224322593512 3 19.15449325624078 25.47801211965564 29.846395077098403 25.52109954700517 4 12.504626161053592 17.139613278009964 37.80338339115408 32.55237716978237 5 13.342007912500828 38.03133888265533 33.854443706229844 14.772209498614009 6 30.6097245814275 36.817418838459744 17.744814418012982 14.828042162099775 7 27.906531085236708 33.63034830113526 20.653810844842205 17.809309768785837 8 26.035278763980298 35.22644123112823 19.497340761426308 19.240939243465167 9 26.640822480931515 17.28120570096462 20.682569315219336 35.39540250288453 10-11 24.989252691715063 27.185739305770255 26.7149382697172 21.110069732797477 12-13 26.02119304419531 24.77013532309461 27.588802175682776 21.619869457027306 14-15 22.97429454821747 25.888908113855262 26.541186146990746 24.595611190936513 16-17 21.441970551160843 29.30382916152729 27.07451678171595 22.17968350559591 18-19 21.448707883636185 27.605075740688594 29.933625816701344 21.01259055897388 20-21 22.525301079143276 26.45639959770024 30.035272691985675 20.98302663117081 22-23 22.229113585813156 26.296113697463287 29.9329471995263 21.541825517197253 24-25 21.9545901919914 26.61213702041675 29.988988337374455 21.444284450217395 26-27 21.553239650527726 26.770571420976264 30.238134288719394 21.43805463977662 28-29 21.326372063856937 27.044045499483122 30.19512064253089 21.434461794129056 30-31 21.680524317250338 26.686122772993965 29.988371956035515 21.644980953720182 32-33 21.486457212373093 26.763315377911322 29.98641559163132 21.763811818084264 34-35 22.045489351537494 26.81827945750453 29.79787384165527 21.338357349302704 36-37 21.86975083723441 26.556935198231947 29.990511092698053 21.58280287183559 38-39 21.614623328968026 27.01398860176869 29.798655633059834 21.572732436203452 40-41 21.80276618151541 26.86068540905525 29.712058840553055 21.62448956887629 42-43 21.563481855615183 26.773300618489948 29.58180076469944 22.081416761195424 44-45 21.7309287895914 26.85864637695101 29.38461476796293 22.025810065494657 46-47 21.914744730517214 27.264689398098028 28.962318584953238 21.858247286431517 48-49 21.919245110584907 27.156207755924566 28.98961941877163 21.9349277147189 50-51 21.67408421859141 27.39320341280048 28.89086990934326 22.04184245926485 52-53 22.060507322884487 27.55410473043634 28.391034441816824 21.994353504862342 54-55 21.77993969406073 27.371204728266246 28.640260164883003 22.208595412790018 56-57 21.821438572919313 27.541504039564536 28.517692476095007 22.119364911421144 58-59 22.14020762684793 27.214500556836818 28.52572911799303 22.119562698322223 60-61 22.09263787497592 27.32510935479855 28.785169081895667 21.79708368832987 62-63 21.874545882753818 27.324529096074922 28.75948948452368 22.041435536647576 64-65 21.647013435344753 27.300555401068717 28.69737531033066 22.355055853255873 66-67 21.828272959567112 27.34325367002355 28.354050804148507 22.474422566260834 68-69 21.723526678925236 27.238328925561017 28.585869368563436 22.452275026950307 70-71 21.944608067034956 27.1846956490366 28.542622564316623 22.32807371961182 72-73 22.05663200471424 27.117824915716938 28.354561106723803 22.47098197284502 74-75 21.706225703841714 27.62203100538652 28.199113695185602 22.472629595586163 76-77 21.873969937452348 27.297296214178868 28.21204185072121 22.616691997647575 78-79 22.073023196399184 27.372151797276345 28.16693091675997 22.3878940895645 80-81 22.01918233487855 27.56375429919303 28.03553523653796 22.381528129390464 82-83 22.101829608718162 27.44693603720783 28.115215230183242 22.336019123890765 84-85 22.18282342828117 27.32638231407426 27.945241437211898 22.545552820432672 86-87 21.963486240462405 27.26090696275234 28.15964900943252 22.615957787352738 88-89 22.051863270925274 27.153769591683112 28.02754587889315 22.766821258498457 90-91 22.27946581044453 27.131062553083535 27.968040078255278 22.621431558216653 92-93 22.520078413956924 26.914122080967275 27.878006248455556 22.68779325662025 94-95 22.324575010938926 26.936114183810993 27.860597272712234 22.878713532537844 96-97 22.236686675223385 26.932992467848404 27.94807130510351 22.882249551824703 98-99 22.297022022511097 26.76012966857742 27.637582439719594 23.30526586919189 100-101 23.125873359625956 25.850918373768963 27.58701877618198 23.4361894904231 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1483.0 1 992.0 2 691.5 3 869.5 4 702.5 5 672.5 6 1214.5 7 2645.5 8 4332.0 9 5117.0 10 4431.5 11 3442.0 12 3273.0 13 3316.0 14 3357.5 15 3415.0 16 3457.0 17 3560.5 18 3754.5 19 3904.5 20 4253.0 21 4879.5 22 5598.5 23 6554.5 24 7712.5 25 9174.5 26 11434.0 27 14326.5 28 16921.0 29 19576.0 30 23055.5 31 26857.0 32 30661.5 33 35005.5 34 39310.0 35 43785.0 36 49898.5 37 55301.0 38 57785.5 39 59130.5 40 60779.0 41 63062.5 42 65088.5 43 67911.5 44 71244.0 45 72816.0 46 72768.5 47 74758.0 48 79766.0 49 79532.5 50 74381.5 51 69325.0 52 64755.5 53 62055.0 54 59817.0 55 55818.0 56 50380.5 57 45747.5 58 42102.0 59 37650.5 60 32007.5 61 26372.0 62 21018.0 63 15992.5 64 12666.5 65 10407.5 66 8122.0 67 6496.0 68 5263.5 69 3829.0 70 2661.0 71 1884.5 72 1376.0 73 1041.5 74 801.0 75 626.5 76 419.0 77 260.5 78 178.0 79 121.0 80 78.5 81 42.0 82 23.0 83 18.5 84 20.0 85 12.5 86 6.0 87 7.0 88 7.5 89 4.5 90 1.5 91 2.0 92 3.5 93 2.5 94 1.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1466960092074409 2 0.05108567066971311 3 0.15150407232929622 4 0.1278143446559881 5 0.19282336478274067 6 0.19337428868211992 7 0.16307347421626067 8 0.06701237976085897 9 0.1195504861652992 10-11 0.09818966406663976 12-13 0.14889970480495793 14-15 0.13577769920156102 16-17 0.13249719780071179 18-19 0.12884106646846763 20-21 0.13074425812086868 22-23 0.19099529911661858 24-25 0.21100385346225622 26-27 0.14509332150015575 28-29 0.12368241541064365 30-31 0.0951345406246275 32-33 0.12260560960731147 34-35 0.09152849328323599 36-37 0.08439152458673195 38-39 0.04935777298529634 40-41 0.057671715466837886 42-43 0.06117759482652408 44-45 0.03796366506631621 46-47 0.049107353031033044 48-49 0.04026752864553857 50-51 0.0521624764730453 52-53 0.027070397055862682 54-55 0.04950802495785432 56-57 0.025793255289119854 58-59 0.05000886486638092 60-61 0.036786691281278705 62-63 0.050885334706302465 64-65 0.03961643676445399 66-67 0.037362657176084293 68-69 0.0654597760444265 70-71 0.05161155257366604 72-73 0.04958315094413331 74-75 0.041644838393986715 76-77 0.020434268267885244 78-79 0.02158620005749642 80-81 0.024365861549819047 82-83 0.020008554345637634 84-85 0.019632924414242685 86-87 0.023389223728192177 88-89 0.06430784425481532 90-91 0.10758041235151349 92-93 0.07978379742828724 94-95 0.07397405448937869 96-97 0.06676195980659566 98-99 0.05494213796536792 100-101 0.1277392186697091 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1996646.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.160713062466314 #Duplication Level Percentage of deduplicated Percentage of total 1 75.48422433935399 37.86342517829171 2 13.156958354949008 13.199248256348323 3 4.49275314052711 6.760791034274244 4 2.1339764678544015 4.281671251444 5 1.1592735743688842 2.907499456240865 6 0.7122466835506397 2.143608091396613 7 0.4733608394670628 1.6620882070460867 8 0.35578951916287227 1.4277324785089358 9 0.261597402040538 1.1809720999477862 >10 1.556984863690041 14.749922508689819 >50 0.13864641920831083 4.7818939847785265 >100 0.07048497511106347 5.946934855182002 >500 0.0026024037464348533 0.9125640230823412 >1k 8.00739614287647E-4 0.7783604780944945 >5k 2.0018490357191174E-4 0.8050706486644662 >10k+ 1.0009245178595587E-4 0.5982174480097981 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11915 0.5967507510094427 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9770 0.48932059063048733 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6265 0.3137762026919143 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3818 0.19122067707545554 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2543 0.12736358873831416 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2110 0.10567722069911242 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.008399085265991E-5 0.0 14-15 0.0 0.0 0.0 5.008399085265991E-5 0.0 16-17 0.0 0.0 0.0 7.512598627898987E-5 0.0 18-19 0.0 0.0 0.0 1.2520997713164978E-4 0.0 20-21 0.0 0.0 0.0 1.5025197255797974E-4 0.0 22-23 0.0 0.0 0.0 4.507559176739392E-4 0.0 24-25 0.0 0.0 0.0 9.015118353478783E-4 0.0 26-27 0.0 0.0 0.0 0.0017779816752694268 0.0 28-29 0.0 0.0 0.0 0.004983357089839661 0.0 30-31 0.0 0.0 0.0 0.014950071269518984 0.0 32-33 0.0 0.0 0.0 0.03498366761058295 0.0 34-35 0.0 0.0 0.0 0.0657853219849688 0.0 36-37 0.0 0.0 0.0 0.11581922884677605 0.0 38-39 0.0 0.0 0.0 0.1931989947141356 0.0 40-41 0.0 0.0 0.0 0.2740846399411814 0.0 42-43 0.0 0.0 0.0 0.3517148257628042 0.0 44-45 0.0 0.0 0.0 0.4427925631283663 0.0 46-47 0.0 0.0 0.0 0.5523262511231335 0.0 48-49 0.0 0.0 0.0 0.6689217818281258 0.0 50-51 0.0 0.0 0.0 0.7872952942083875 0.0 52-53 0.0 0.0 0.0 0.9066454444102761 0.0 54-55 0.0 0.0 0.0 1.03353323523549 0.0 56-57 0.0 0.0 0.0 1.1769237010466553 0.0 58-59 0.0 0.0 0.0 1.3340371803514492 0.0 60-61 0.0 0.0 0.0 1.504097371291656 0.0 62-63 0.0 0.0 0.0 1.6711775647761296 0.0 64-65 0.0 0.0 0.0 1.8393596060593616 5.008399085265991E-5 66-67 0.0 0.0 0.0 2.0240192803331185 5.008399085265991E-5 68-69 0.0 0.0 0.0 2.229288516842745 5.008399085265991E-5 70-71 0.0 0.0 0.0 2.4544410977208777 5.008399085265991E-5 72-73 0.0 0.0 0.0 2.670979232172353 5.008399085265991E-5 74-75 0.0 0.0 0.0 2.8926259336907996 5.008399085265991E-5 76-77 0.0 0.0 0.0 3.127444724803495 5.008399085265991E-5 78-79 0.0 0.0 0.0 3.393065170290577 5.008399085265991E-5 80-81 0.0 0.0 0.0 3.676240054571516 5.008399085265991E-5 82-83 0.0 0.0 0.0 3.9540309098357946 5.008399085265991E-5 84-85 0.0 0.0 0.0 4.231746639113794 7.512598627898987E-5 86-87 0.0 0.0 0.0 4.51637395912946 1.0016798170531982E-4 88-89 0.0 0.0 0.0 4.827019912393084 1.0016798170531982E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14720 0.0 30.310806 1 GGTATCA 10290 0.0 28.78331 1 GGACCGA 515 0.0 27.701384 6 GTACATG 10950 0.0 25.874296 1 ATCAACG 17410 0.0 25.407957 3 TATCAAC 17525 0.0 25.322655 2 CAACGCA 17570 0.0 25.122438 5 TACATGG 11285 0.0 25.03632 2 TCAACGC 17700 0.0 24.937922 4 GAGTACT 8570 0.0 24.713509 12-13 AACGCAG 17890 0.0 24.640915 6 ACATGGG 11430 0.0 23.553518 3 AGGACCG 675 0.0 23.253897 5 GTACTTT 9210 0.0 22.915869 14-15 ACGCAGA 19625 0.0 22.266384 7 CGCAGAG 19750 0.0 22.074533 8 AGTACTT 9065 0.0 21.44979 12-13 CATGGGG 5430 0.0 21.110708 4 AGAGTAC 15425 0.0 21.050003 10-11 GCAGAGT 20360 0.0 20.876085 9 >>END_MODULE