FastQCFastQC Report
Thu 26 May 2016
SRR938276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996646
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116160.5817756377444975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100270.502192176279621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63840.31973619760338085No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37730.18896689748708584No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.13407484351257057No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22560.11298948336360076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97200.030.152881
GTATCAA135250.028.1674141
GTACATG106400.025.1795161
TACATGG107950.024.3333262
GAGTACT83800.023.54659312-13
ATCAACG160800.023.5417443
CAACGCA163000.023.2787825
TCAACGC163500.023.2366394
TATCAAC163200.023.226392
ACATGGG109900.023.0354063
AACGCAG166000.022.8866926
GTACTTT87950.022.21898514-15
GTACCTG16650.021.6824931
GGACCGA5950.021.550036
GTATAGG4100.020.8544391
ACGCAGA184250.020.568217
CGCAGAG185100.020.4224458
CATGGGA56050.020.4193784
AGTACTT87100.020.41901212-13
TACCTGG16400.019.6953662
Produced by FastQC (version 0.11.5)