##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938276_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1996646 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56994830330464 34.0 31.0 34.0 31.0 34.0 2 32.776340422889184 34.0 31.0 34.0 31.0 34.0 3 32.824694512697796 34.0 31.0 34.0 31.0 34.0 4 36.26366065892502 37.0 35.0 37.0 35.0 37.0 5 36.244421394678874 37.0 37.0 37.0 35.0 37.0 6 36.28237754714657 37.0 37.0 37.0 35.0 37.0 7 36.24496029842045 37.0 37.0 37.0 35.0 37.0 8 36.240322520867494 37.0 37.0 37.0 35.0 37.0 9 37.93428980400131 39.0 38.0 39.0 35.0 39.0 10-11 37.913846019775164 39.0 38.0 39.0 35.0 39.0 12-13 37.83351255054727 39.0 38.0 39.0 35.0 39.0 14-15 39.11151926781212 40.0 38.5 41.0 36.0 41.0 16-17 39.00395388065786 40.0 38.0 41.0 36.0 41.0 18-19 39.26776654449512 40.5 39.0 41.0 36.0 41.0 20-21 39.26491801751537 41.0 39.0 41.0 36.0 41.0 22-23 39.16959991906427 40.5 39.0 41.0 36.0 41.0 24-25 39.07608459386391 40.5 39.0 41.0 35.0 41.0 26-27 38.91250902763935 40.0 38.0 41.0 35.0 41.0 28-29 38.80314387227381 40.0 38.0 41.0 35.0 41.0 30-31 38.58005450139885 40.0 38.0 41.0 34.5 41.0 32-33 38.376869760588505 40.0 38.0 41.0 34.0 41.0 34-35 38.22237066560622 40.0 38.0 41.0 34.0 41.0 36-37 38.22084510724485 40.0 38.0 41.0 34.0 41.0 38-39 38.09454254785275 40.0 38.0 41.0 34.0 41.0 40-41 37.9419338731052 40.0 37.5 41.0 33.0 41.0 42-43 37.7169798752508 40.0 37.0 41.0 33.0 41.0 44-45 37.43243819885949 40.0 37.0 41.0 33.0 41.0 46-47 37.0193281633299 40.0 36.5 41.0 31.5 41.0 48-49 36.62922821571776 40.0 36.0 41.0 30.5 41.0 50-51 36.27654426473195 39.0 35.5 41.0 30.0 41.0 52-53 35.807284315797595 39.0 35.0 40.0 28.5 41.0 54-55 35.52807182645296 38.5 35.0 40.0 28.0 41.0 56-57 35.21752253529168 38.0 35.0 40.0 27.0 41.0 58-59 34.816775482484125 38.0 34.0 40.0 26.5 41.0 60-61 34.36329073856858 37.5 34.0 40.0 26.0 41.0 62-63 33.93494039504249 37.0 33.0 40.0 24.5 41.0 64-65 33.478697776170634 36.5 33.0 39.0 23.0 41.0 66-67 32.945808120217606 36.0 32.0 39.0 22.0 40.0 68-69 32.384308485329896 35.0 31.5 38.5 20.0 40.0 70-71 31.807485903860773 35.0 31.0 38.0 18.0 40.0 72-73 31.236234164694192 35.0 30.5 37.0 15.0 39.0 74-75 30.65430501951773 34.0 30.0 36.5 9.5 39.0 76-77 29.258774214357476 32.5 28.5 35.0 8.0 37.5 78-79 29.962035834093776 34.0 29.5 36.0 7.0 38.0 80-81 29.629817704290094 34.0 29.0 35.0 2.0 37.0 82-83 29.087406079996157 34.0 29.0 35.0 2.0 37.0 84-85 28.508784481575603 34.0 28.5 35.0 2.0 36.0 86-87 27.894053077010145 33.0 26.5 35.0 2.0 36.0 88-89 27.192406665978847 33.0 26.0 35.0 2.0 35.0 90-91 26.668641561899307 32.5 25.0 35.0 2.0 35.0 92-93 25.871595916351723 32.0 23.5 34.0 2.0 35.0 94-95 25.082975650165327 31.5 19.5 34.0 2.0 35.0 96-97 24.066919223537873 31.0 8.5 34.0 2.0 35.0 98-99 22.675196053782194 31.0 2.0 34.0 2.0 35.0 100-101 18.242132305876957 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11376718520650542 1101 2 0.17709299138940082 1101 3 0.16117644375189144 1101 4 0.08806046968040704 1101 5 0.13399805869750026 1101 6 0.11759668699957615 1101 7 0.09551241383432085 1101 8 0.07824708919191892 1101 9 -0.06327598800849188 1101 10-11 0.15421686475276886 1101 12-13 0.14661048521484332 1101 14-15 0.11879639949734866 1101 16-17 0.2587678799202493 1101 18-19 0.25328155313953715 1101 20-21 0.2542816734912918 1101 22-23 0.3035951786689708 1101 24-25 0.3018192810137563 1101 26-27 0.2406699826573373 1101 28-29 0.20509764643089312 1101 30-31 0.2371271362864249 1101 32-33 0.17253113637939776 1101 34-35 0.1087174529609598 1101 36-37 0.21310131124527487 1101 38-39 0.24986802689796406 1101 40-41 0.19286170386633472 1101 42-43 0.1930889264273432 1101 44-45 0.22046275432712292 1101 46-47 0.05576418645098613 1101 48-49 0.033941094104008585 1101 50-51 -0.16223603241184037 1101 52-53 -0.3197411028303705 1101 54-55 -0.1758159817816889 1101 56-57 -0.1570419838618946 1101 58-59 -0.12532784933668495 1101 60-61 -0.030855396930292045 1101 62-63 -0.22351445652916624 1101 64-65 -0.22540034848821477 1101 66-67 -0.11926002053073859 1101 68-69 -0.014438829032279443 1101 70-71 -0.06715651143131396 1101 72-73 -0.22480874160533304 1101 74-75 -0.0973208095958853 1101 76-77 -0.19236187716337483 1101 78-79 -0.345529242457264 1101 80-81 -0.31314234989068623 1101 82-83 -0.581850124794375 1101 84-85 -0.5493030916190911 1101 86-87 -0.5347773491051413 1101 88-89 -0.32077203464887916 1101 90-91 -0.42499799386403936 1101 92-93 -0.6094230846407314 1101 94-95 -0.7660513578416754 1101 96-97 -0.6723752433231489 1101 98-99 -0.7886050203555435 1101 100-101 -1.1790749830432432 1102 1 0.13051108874947914 1102 2 0.1254829894329248 1102 3 0.13188702301386712 1102 4 0.11970203801241297 1102 5 0.1011358849101498 1102 6 0.13653432223711093 1102 7 0.1507090393566557 1102 8 0.18356336617007685 1102 9 0.22997556767944616 1102 10-11 0.21267233163436572 1102 12-13 0.18755807510309097 1102 14-15 0.20835722974266702 1102 16-17 0.15806646979974204 1102 18-19 0.18093293887714879 1102 20-21 0.19879358461485452 1102 22-23 0.24092946249770364 1102 24-25 0.2973093555641313 1102 26-27 0.23482009230742307 1102 28-29 0.22068810701964026 1102 30-31 0.21770293792928186 1102 32-33 0.15978978939163113 1102 34-35 0.27464752219673727 1102 36-37 0.2213736143058398 1102 38-39 0.1708217782156609 1102 40-41 0.1613369398841229 1102 42-43 0.2018713359842792 1102 44-45 0.04716014053777684 1102 46-47 0.13164740984223045 1102 48-49 0.316478953313883 1102 50-51 0.30033875887338013 1102 52-53 0.3447002584659131 1102 54-55 0.45463841634009583 1102 56-57 0.39284533644191555 1102 58-59 0.42676908075299735 1102 60-61 0.1775856154130011 1102 62-63 0.43085599722653 1102 64-65 0.3582686148439791 1102 66-67 0.08336285764524831 1102 68-69 0.13777968708526345 1102 70-71 -0.03890190422974271 1102 72-73 0.06772300727699943 1102 74-75 0.13552884422522027 1102 76-77 0.10141037041512391 1102 78-79 0.08994639215536537 1102 80-81 0.04147072840035193 1102 82-83 0.25855954456171304 1102 84-85 -0.2117584139552946 1102 86-87 -0.13706273571293792 1102 88-89 -0.1343145134707875 1102 90-91 -0.44218484140224135 1102 92-93 -0.3231040496198645 1102 94-95 -0.28006660226937896 1102 96-97 -0.478206992497487 1102 98-99 -0.44033715586027 1102 100-101 -0.9188350742090385 1103 1 0.18835543753205286 1103 2 0.16020643760576547 1103 3 0.10339600602558363 1103 4 0.06727261881877666 1103 5 0.07049790775131015 1103 6 0.0724738379828409 1103 7 0.07153156546374362 1103 8 0.03077442281125542 1103 9 0.15113928527474485 1103 10-11 0.13677436995644143 1103 12-13 0.15537030775155358 1103 14-15 0.18526586511250542 1103 16-17 0.17706591302954422 1103 18-19 0.30783580284867895 1103 20-21 0.22680981921880772 1103 22-23 0.19781491042611066 1103 24-25 0.1915326950988927 1103 26-27 0.21156526130754116 1103 28-29 0.2838733756952081 1103 30-31 0.3856822016346513 1103 32-33 0.30933605563642885 1103 34-35 0.32486887229654826 1103 36-37 0.33491365423034125 1103 38-39 0.326456360434328 1103 40-41 0.3063015646074305 1103 42-43 0.2972027839941589 1103 44-45 0.26539775919093955 1103 46-47 0.23260868715452432 1103 48-49 0.08864434800963039 1103 50-51 0.02803773631740114 1103 52-53 0.05902809425945321 1103 54-55 0.030322496786602926 1103 56-57 0.057536770328844966 1103 58-59 0.28150162736552176 1103 60-61 0.14040762548918906 1103 62-63 0.15667010388197156 1103 64-65 0.06923885479834979 1103 66-67 0.07805401993697103 1103 68-69 0.07117961782848425 1103 70-71 -0.20296748089428363 1103 72-73 -0.30748612103815987 1103 74-75 -0.3689088518325754 1103 76-77 -0.18146232611796265 1103 78-79 -0.33728934570093116 1103 80-81 -0.25985516548739795 1103 82-83 -0.22507599503007825 1103 84-85 -0.22322329669406216 1103 86-87 -0.29812531804023834 1103 88-89 -0.6523187299864937 1103 90-91 -0.6501458063800385 1103 92-93 -0.6681852905384034 1103 94-95 -0.7632102150289235 1103 96-97 -0.8354633376406859 1103 98-99 -0.8452601586169592 1103 100-101 -1.4684312460744913 1104 1 0.04644571034348388 1104 2 0.15284271365189994 1104 3 0.14452503701609487 1104 4 0.13601349275214147 1104 5 0.04547727832187576 1104 6 0.013274527045076923 1104 7 0.022423935639793058 1104 8 0.09288715930310332 1104 9 0.19649636694527572 1104 10-11 0.1674205072627828 1104 12-13 0.10565016069711675 1104 14-15 0.14688396682777238 1104 16-17 0.09285964702700511 1104 18-19 0.16049373783100407 1104 20-21 0.24318180594681138 1104 22-23 0.20221161160351642 1104 24-25 0.26320962801748493 1104 26-27 0.24503790550529203 1104 28-29 0.07412874052568696 1104 30-31 0.1748029085521594 1104 32-33 0.14456389098450728 1104 34-35 0.10097865701175124 1104 36-37 0.14075842347150314 1104 38-39 0.13128906951457253 1104 40-41 0.16570646254480437 1104 42-43 0.2044813182280123 1104 44-45 0.3117791607975988 1104 46-47 0.23360440094850077 1104 48-49 0.2554253868526857 1104 50-51 0.2204404479281976 1104 52-53 0.16848965436299324 1104 54-55 0.2825274487927274 1104 56-57 0.20663968485464324 1104 58-59 0.25730790405607706 1104 60-61 0.07642495892520884 1104 62-63 0.18422819242584154 1104 64-65 -0.011179140665532827 1104 66-67 -0.011994028951242797 1104 68-69 0.31641032950842884 1104 70-71 -0.1417904167332189 1104 72-73 -0.29750194872230296 1104 74-75 -0.24568113593872098 1104 76-77 -0.2563307300052564 1104 78-79 -0.17099382539135632 1104 80-81 -0.034650639429873564 1104 82-83 -0.050141576968602664 1104 84-85 -0.2544376274282172 1104 86-87 -0.1594792058821568 1104 88-89 -0.44324083797625136 1104 90-91 -0.8353088063784675 1104 92-93 -0.4721066896616719 1104 94-95 -0.6164800385353146 1104 96-97 -0.9685801340042488 1104 98-99 -1.016861646765058 1104 100-101 -1.183235553342179 1105 1 0.12235831662982832 1105 2 0.07577941936893495 1105 3 0.09246614311163626 1105 4 0.06364666899952454 1105 5 0.09023579052827557 1105 6 0.06315241471110511 1105 7 0.009660641444689588 1105 8 0.05761764966488414 1105 9 0.12623616033688734 1105 10-11 0.1785563203692675 1105 12-13 0.20616022387221022 1105 14-15 0.10031244010290408 1105 16-17 0.08448150207428995 1105 18-19 0.12277422044635955 1105 20-21 0.18718673319926182 1105 22-23 0.22583073475524884 1105 24-25 0.2079288662965908 1105 26-27 0.18932369551892947 1105 28-29 0.06557794844291465 1105 30-31 0.08248413898291318 1105 32-33 0.05848663000266896 1105 34-35 0.09329578650646653 1105 36-37 0.09959284594122408 1105 38-39 0.23467219180057697 1105 40-41 0.10496527772568953 1105 42-43 0.14328135184954505 1105 44-45 -0.04690923646593603 1105 46-47 0.04028281367266118 1105 48-49 0.03405178929943986 1105 50-51 -0.0594469082394653 1105 52-53 -0.20565629879705938 1105 54-55 -0.203872526137566 1105 56-57 -0.2653630716420352 1105 58-59 -0.13504155833628317 1105 60-61 -0.3715707675091906 1105 62-63 -0.2725568690725666 1105 64-65 -0.22898733336491262 1105 66-67 -0.4360339776520519 1105 68-69 -0.3683242961840989 1105 70-71 -0.5132040350334073 1105 72-73 -0.6439356213265306 1105 74-75 -0.5271532141093545 1105 76-77 -0.5412107951630709 1105 78-79 -0.4536592042090355 1105 80-81 -0.3603991650468146 1105 82-83 -0.42728213047617913 1105 84-85 -0.47232782385349736 1105 86-87 -0.6810209989346276 1105 88-89 -0.9369363622421822 1105 90-91 -0.9586983483847291 1105 92-93 -0.977597278416205 1105 94-95 -0.9982533632504449 1105 96-97 -1.0655823413688275 1105 98-99 -0.9581270638316148 1105 100-101 -1.563474822642121 1106 1 0.10336588678417513 1106 2 0.1647673061423447 1106 3 0.14178419245601503 1106 4 0.07492291718106969 1106 5 0.07068619144521904 1106 6 0.14550753846390307 1106 7 0.1600219442655586 1106 8 0.1094894933929993 1106 9 0.05257655033086195 1106 10-11 0.0877905432957391 1106 12-13 0.03467914558441976 1106 14-15 0.08281916287018731 1106 16-17 0.10752351141024263 1106 18-19 0.1791719927490476 1106 20-21 0.23112243586925985 1106 22-23 0.20577983642699849 1106 24-25 0.17564917468670416 1106 26-27 0.13653864528797754 1106 28-29 0.1643894636003651 1106 30-31 0.16521152525915994 1106 32-33 0.2575752909351863 1106 34-35 0.17784575907660383 1106 36-37 0.1511844212974509 1106 38-39 0.0314176193391873 1106 40-41 -0.024383091421590564 1106 42-43 -0.016704743410478784 1106 44-45 -0.035789118125642005 1106 46-47 -0.11556170794550269 1106 48-49 -0.2136745316202422 1106 50-51 -0.17272936365367997 1106 52-53 -0.12077921890266197 1106 54-55 -0.3899078139307832 1106 56-57 -0.4414069680061061 1106 58-59 -0.17196075532683608 1106 60-61 -0.4260683478027829 1106 62-63 -0.4748301195611546 1106 64-65 -0.4639437639551005 1106 66-67 -0.5319777549900806 1106 68-69 -0.6071317764566331 1106 70-71 -1.0552083197260664 1106 72-73 -1.0600678749352745 1106 74-75 -0.6958293126511634 1106 76-77 -0.7822843010923322 1106 78-79 -0.8721389159015054 1106 80-81 -0.7750688494073792 1106 82-83 -0.7285377205367674 1106 84-85 -0.7381984222165237 1106 86-87 -1.0024210121597434 1106 88-89 -1.2276256356934958 1106 90-91 -1.33726316002479 1106 92-93 -1.2190112086267817 1106 94-95 -1.3070895163091194 1106 96-97 -1.573200253074038 1106 98-99 -1.7119508673259851 1106 100-101 -2.2331109666391455 1107 1 -0.059839459504559045 1107 2 -0.014634658157419267 1107 3 0.03619105438655623 1107 4 0.03544779452165869 1107 5 0.006668800483097925 1107 6 -0.016602946838830235 1107 7 -0.017843497701889532 1107 8 0.025761871630024302 1107 9 0.03263291681357572 1107 10-11 0.04407235603132165 1107 12-13 0.03708696517669097 1107 14-15 0.06728121060459813 1107 16-17 -0.007382231061832556 1107 18-19 0.05881708943353203 1107 20-21 1.7263439822556848E-4 1107 22-23 -0.0159426318914484 1107 24-25 0.06851240834742356 1107 26-27 0.15259751743358407 1107 28-29 0.01915448664546915 1107 30-31 0.021908816694427458 1107 32-33 0.016239341888031333 1107 34-35 0.033225992326272547 1107 36-37 -0.018472842938471956 1107 38-39 -0.14647487985183005 1107 40-41 -0.20820932173267437 1107 42-43 -0.155123759773808 1107 44-45 -0.21016007867347497 1107 46-47 -0.23195915068257023 1107 48-49 -0.23109993020867847 1107 50-51 -0.32436870343556734 1107 52-53 -0.2874276652167609 1107 54-55 -0.1526009314476866 1107 56-57 -0.20855878800068695 1107 58-59 -0.2204270753695141 1107 60-61 -0.2982308922968855 1107 62-63 -0.3694282951936927 1107 64-65 -0.48401093146281227 1107 66-67 -0.38687369126841276 1107 68-69 -0.30050756685552926 1107 70-71 -0.47835733232872 1107 72-73 -0.627833921235375 1107 74-75 -0.6578997313985973 1107 76-77 -0.6239150918847862 1107 78-79 -0.6623557891140877 1107 80-81 -0.48267181990789965 1107 82-83 -0.6558915350040202 1107 84-85 -0.9254795046803821 1107 86-87 -0.8841754790613763 1107 88-89 -1.1094965306480624 1107 90-91 -1.2157174655669856 1107 92-93 -1.2596716563227055 1107 94-95 -1.4197869520495772 1107 96-97 -1.601453460584903 1107 98-99 -1.7408586410029692 1107 100-101 -1.8217173611083197 1108 1 -0.1068527021904302 1108 2 -0.053138611267499414 1108 3 -0.050347522316378956 1108 4 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-0.5292611223802979 2316 48-49 -0.2656974399731169 2316 50-51 -0.1953133072364608 2316 52-53 -0.05208501558674783 2316 54-55 -0.12108736097484751 2316 56-57 -0.06092886499051531 2316 58-59 -0.1289818809285137 2316 60-61 0.04629129482416516 2316 62-63 0.08592227001111752 2316 64-65 0.19981564691419607 2316 66-67 0.16741568105714322 2316 68-69 0.38369519725464585 2316 70-71 0.45202853591222336 2316 72-73 0.6674394617715755 2316 74-75 0.8511894645186473 2316 76-77 0.6358064238017285 2316 78-79 0.7501518165349665 2316 80-81 0.7335719227981592 2316 82-83 0.9609027355732671 2316 84-85 0.9703761824926822 2316 86-87 1.0541033429642965 2316 88-89 1.1029547183883217 2316 90-91 1.2624890547007332 2316 92-93 1.109734540197291 2316 94-95 1.7217456272023526 2316 96-97 2.1170295846956044 2316 98-99 2.52376183889033 2316 100-101 3.075452838711854 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 12.0 5 36.0 6 73.0 7 172.0 8 363.0 9 743.0 10 1328.0 11 2227.0 12 3573.0 13 5649.0 14 7346.0 15 10425.0 16 14354.0 17 20542.0 18 21141.0 19 15144.0 20 13571.0 21 14241.0 22 15381.0 23 17060.0 24 19214.0 25 21936.0 26 25721.0 27 30244.0 28 36710.0 29 44523.0 30 53728.0 31 65159.0 32 82512.0 33 107119.0 34 145877.0 35 209281.0 36 304484.0 37 414339.0 38 258249.0 39 14163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.908319537978755 19.67447137286527 15.384746386702364 22.03246270245361 2 15.316190337842405 20.518197005026686 40.69622234633793 23.469390310792974 3 19.23683377477871 25.28264859211649 30.099295156656343 25.381222476448457 4 12.717076537353142 17.371181471327414 37.481556570368504 32.430185420950934 5 13.271114913088205 37.951399914455514 34.122132281423326 14.655352891032955 6 30.77369987388649 36.91885361917727 17.4995266987009 14.807919808235342 7 27.992693747414414 33.55442076362059 20.527174070916928 17.925711418048067 8 25.988532769454377 35.37382189932517 19.396678229390687 19.240967101829767 9 26.381692097647758 17.156571570523766 20.765724119348146 35.696012212480326 10-11 25.031477788250893 27.205122991256335 26.618714584357967 21.144684636134798 12-13 25.95046545999829 24.672384977264155 27.633103228595026 21.74404633414253 14-15 22.852724018178485 25.808530906329914 26.454914892274346 24.883830183217256 16-17 21.46562559398015 29.34531773978141 27.06127859212274 22.1277780741157 18-19 21.402216517099177 27.572013266247495 29.974041467541067 21.05172874911226 20-21 22.38148374824581 26.483963606968885 30.066571640641353 21.06798100414395 22-23 22.180621902931215 26.318085429264876 29.940410067683505 21.5608826001204 24-25 21.908263129952218 26.59407586973725 30.025493594045816 21.472167406264713 26-27 21.56128782438749 26.785926734280714 30.263492398241432 21.389293043090365 28-29 21.377900992479397 26.950210742218317 30.276857424061426 21.39503084124086 30-31 21.652569697045585 26.562497260951503 30.104165231926977 21.680767810075942 32-33 21.40231668508088 26.79568636603584 30.003916568084676 21.7980803807986 34-35 22.01555054779542 26.73461036225674 29.84436930021533 21.40546978973251 36-37 21.820424241437728 26.548734174837758 30.075414856839487 21.555426726885027 38-39 21.534906993319066 26.99202184538838 29.882619102594088 21.59045205869846 40-41 21.764755673178442 26.787048705090882 29.777224945757464 21.670970675973212 42-43 21.556050961475375 26.704137652280142 29.62312309078533 22.116688295459156 44-45 21.747350744035433 26.813097267391733 29.473284065699197 21.96626792287364 46-47 21.933256420844703 27.191235882856287 28.98196796971314 21.89353972658587 48-49 21.83852060907553 27.118335497393737 29.074303859122796 21.968840034407936 50-51 21.64325214507203 27.325270103022557 28.963593753834733 22.067883998070677 52-53 22.034535446429086 27.495422546939714 28.44294468663574 22.027097319995462 54-55 21.747148913172236 27.311629919826213 28.641773966171886 22.29944720082966 56-57 21.808444691680165 27.439349535176532 28.579811956696794 22.17239381644651 58-59 22.098801520023382 27.146985439746995 28.57793751833213 22.17627552189749 60-61 22.00796363772961 27.31723075767251 28.78223958528981 21.892566019308067 62-63 21.791148105326645 27.35133461708783 28.742833984895512 22.114683292690014 64-65 21.595945913451907 27.29753111900163 28.66825501150181 22.438267956044662 66-67 21.72488177944569 27.358569246401416 28.275806969083206 22.64074200506969 68-69 21.565797288634727 27.24681870866588 28.586419225798725 22.600964776900664 70-71 21.8329049577193 27.288169469433715 28.41738294976559 22.46154262308139 72-73 21.931785853097566 27.223200583410467 28.192228940524988 22.65278462296698 74-75 21.523400243650265 27.696182545343795 28.1000038070354 22.680413403970537 76-77 21.55186556743482 27.27920079850018 28.343851010085196 22.825082623979803 78-79 21.785142904664013 27.32420939094591 28.283227798558542 22.607419905831534 80-81 21.675769709707765 27.490730824753733 28.2165365492521 22.616962916286397 82-83 21.676857855524023 27.453682468290996 28.28462613922828 22.5848335369567 84-85 21.702324311529633 27.275011177585217 28.17630652507975 22.846357985805408 86-87 21.45554317526418 27.2254714920411 28.36975777603652 22.9492275566582 88-89 21.533942145098383 27.19965373924792 28.18856583074652 23.077838284907173 90-91 21.644503093080996 27.185070849289705 28.243677069117883 22.926748988511413 92-93 21.861737688304007 27.0367992633785 28.12878151613075 22.972681532186737 94-95 21.55084494455865 27.039903944410383 28.091401081176393 23.317850029854576 96-97 21.358220977272317 27.093815056814197 28.230023863766146 23.31794010214734 98-99 21.342354492484663 26.919419410018197 28.023296730268093 23.714929367229047 100-101 21.696710234347844 26.494915434219212 27.870793625033436 23.937580706399512 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1444.0 1 974.0 2 651.5 3 834.0 4 687.5 5 630.0 6 1220.0 7 2784.5 8 4454.0 9 5200.0 10 4516.0 11 3383.0 12 3128.0 13 3183.5 14 3288.5 15 3351.0 16 3358.5 17 3407.5 18 3568.5 19 3807.0 20 4078.0 21 4645.5 22 5496.5 23 6453.0 24 7542.0 25 9019.0 26 11359.5 27 14235.5 28 16482.0 29 19157.0 30 22833.5 31 26742.5 32 30433.0 33 34616.5 34 39336.0 35 43861.0 36 50006.0 37 55392.0 38 58107.0 39 59873.0 40 61122.0 41 63158.0 42 65093.5 43 68115.5 44 72226.0 45 74181.5 46 73164.0 47 74990.0 48 81798.0 49 81269.0 50 74548.0 51 69447.5 52 65015.5 53 62314.5 54 60377.5 55 56286.0 56 50827.0 57 46253.0 58 42188.5 59 37560.5 60 31379.5 61 25069.0 62 19933.5 63 15235.5 64 12083.0 65 9922.5 66 7617.5 67 6165.0 68 4922.5 69 3429.5 70 2329.5 71 1582.5 72 1162.0 73 936.5 74 714.0 75 548.0 76 374.0 77 204.0 78 121.5 79 72.0 80 38.0 81 22.0 82 12.5 83 7.5 84 6.0 85 3.0 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010066882161384643 2 0.015075281246650633 3 0.008714614408362825 4 0.0 5 0.001202015780463838 6 0.001602687707285117 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.515958262005383E-4 14-15 0.0 16-17 0.0025793255289119853 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0033055433962755542 26-27 0.018255614665794537 28-29 0.006561002801698449 30-31 0.0029299134648806046 32-33 0.0 34-35 0.009465874271152724 36-37 0.001176973785037508 38-39 0.0034557953688335334 40-41 0.0029048714694542748 42-43 0.003956635277360132 44-45 2.5041995426329953E-5 46-47 2.253779588369696E-4 48-49 6.761338765109088E-4 50-51 0.004657811149297372 52-53 0.008789740394641815 54-55 0.008539320440378514 56-57 0.017554438793857298 58-59 0.024666365494935005 60-61 0.0035559633505388537 62-63 0.020935108176411844 64-65 0.021936787993465042 66-67 0.013597803516497167 68-69 0.03207879614112867 70-71 0.017278976844167668 72-73 0.00550923899379259 74-75 0.01712872487160969 76-77 0.020208890309048273 78-79 0.027170565037568 80-81 0.01222049376804902 82-83 0.03618568339104678 84-85 0.02321392976020787 86-87 0.03215392212740766 88-89 0.023163845769355207 90-91 0.02894854671283743 92-93 0.02531745737601959 94-95 0.015125365237503293 96-97 0.01572637312773521 98-99 0.011444191909832788 100-101 0.010617806060763901 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1996646.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.05835785663436 #Duplication Level Percentage of deduplicated Percentage of total 1 73.74252740274875 35.439447711739646 2 13.959983843092877 13.417877984083823 3 4.842085404748839 6.9810801946141785 4 2.3094024311345214 4.439443538817769 5 1.2567632665294137 3.019898940197165 6 0.7848501348638613 2.2631165187069113 7 0.528902883391444 1.779274282900224 8 0.3679694627026986 1.4147206495103808 9 0.2738747537112687 1.1845773829578374 >10 1.6954703458376705 15.425069861782802 >50 0.15828184005534782 5.244190934587213 >100 0.07571742576058824 6.172152413061312 >500 0.003023833931533036 1.0191977623452428 >1k 8.341610845608374E-4 0.7955044238768243 >5k 1.0427013557010468E-4 0.31990552705698555 >10k+ 2.0854027114020936E-4 1.0845418737616446 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11616 0.5817756377444975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10027 0.502192176279621 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6384 0.31973619760338085 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3773 0.18896689748708584 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2677 0.13407484351257057 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2256 0.11298948336360076 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.008399085265991E-5 0.0 14-15 0.0 0.0 0.0 5.008399085265991E-5 0.0 16-17 0.0 0.0 0.0 7.512598627898987E-5 0.0 18-19 0.0 0.0 0.0 1.2520997713164978E-4 0.0 20-21 0.0 0.0 0.0 1.5025197255797974E-4 0.0 22-23 0.0 0.0 0.0 4.257139222476092E-4 0.0 24-25 0.0 0.0 0.0 8.514278444952184E-4 0.0 26-27 0.0 0.0 0.0 0.0017779816752694268 0.0 28-29 0.0 0.0 0.0 0.004933273098987001 0.0 30-31 0.0 0.0 0.0 0.014950071269518982 0.0 32-33 0.0 0.0 0.0 0.03533425554655157 0.0 34-35 0.0 0.0 0.0 0.06648649785690602 0.0 36-37 0.0 0.0 0.0 0.11712141260894521 0.0 38-39 0.0 0.0 0.0 0.19487680840769972 0.0 40-41 0.0 0.0 0.0 0.2757874956301718 0.0 42-43 0.0 0.0 0.0 0.353743227392337 0.0 44-45 0.0 0.0 0.0 0.44512146870301494 0.0 46-47 0.0 0.0 0.0 0.5549306186474718 0.0 48-49 0.0 0.0 0.0 0.6723024512106803 0.0 50-51 0.0 0.0 0.0 0.7916025174217163 0.0 52-53 0.0 0.0 0.0 0.9115035915229841 0.0 54-55 0.0 0.0 0.0 1.0382411303756398 0.0 56-57 0.0 0.0 0.0 1.1825331080221533 0.0 58-59 0.0 0.0 0.0 1.3408986870982638 0.0 60-61 0.0 0.0 0.0 1.5123111457914922 0.0 62-63 0.0 0.0 0.0 1.6807686490244138 0.0 64-65 0.0 0.0 0.0 1.8496769081750095 0.0 66-67 0.0 0.0 0.0 2.0355636402246566 0.0 68-69 0.0 0.0 0.0 2.2420849765056 0.0 70-71 0.0 0.0 0.0 2.4698920088989236 0.0 72-73 0.0 0.0 0.0 2.6893350148198527 0.0 74-75 0.0 0.0 0.0 2.9138114618214743 0.0 76-77 0.0 0.0 0.0 3.1527120981886627 0.0 78-79 0.0 0.0 0.0 3.4236915306969786 0.0 80-81 0.0 0.0 0.0 3.712425737962563 0.0 82-83 0.0 0.0 0.0 3.996401966097145 0.0 84-85 0.0 0.0 0.0 4.281505084025912 0.0 86-87 0.0 0.0 0.0 4.57341962471064 0.0 88-89 0.0 0.0 0.0 4.893055654332315 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9720 0.0 30.15288 1 GTATCAA 13525 0.0 28.167414 1 GTACATG 10640 0.0 25.179516 1 TACATGG 10795 0.0 24.333326 2 GAGTACT 8380 0.0 23.546593 12-13 ATCAACG 16080 0.0 23.541744 3 CAACGCA 16300 0.0 23.278782 5 TCAACGC 16350 0.0 23.236639 4 TATCAAC 16320 0.0 23.22639 2 ACATGGG 10990 0.0 23.035406 3 AACGCAG 16600 0.0 22.886692 6 GTACTTT 8795 0.0 22.218985 14-15 GTACCTG 1665 0.0 21.682493 1 GGACCGA 595 0.0 21.55003 6 GTATAGG 410 0.0 20.854439 1 ACGCAGA 18425 0.0 20.56821 7 CGCAGAG 18510 0.0 20.422445 8 CATGGGA 5605 0.0 20.419378 4 AGTACTT 8710 0.0 20.419012 12-13 TACCTGG 1640 0.0 19.695366 2 >>END_MODULE