##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938275_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 234342 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.349301448310587 33.0 31.0 34.0 30.0 34.0 2 31.36231234691178 33.0 31.0 34.0 30.0 34.0 3 31.30080822046411 33.0 31.0 34.0 28.0 34.0 4 34.90059400363571 37.0 35.0 37.0 33.0 37.0 5 34.97393979739014 37.0 35.0 37.0 33.0 37.0 6 35.13357400722022 37.0 35.0 37.0 33.0 37.0 7 35.087820365107405 37.0 35.0 37.0 33.0 37.0 8 35.06691502163505 37.0 35.0 37.0 33.0 37.0 9 36.570729958778195 39.0 37.0 39.0 33.0 39.0 10-11 36.5157696870386 39.0 37.0 39.0 32.5 39.0 12-13 36.351989826834284 39.0 37.0 39.0 32.0 39.0 14-15 37.11804542079525 40.0 37.0 41.0 32.0 41.0 16-17 37.061973525872446 39.5 37.0 41.0 32.0 41.0 18-19 37.37721151138081 40.0 37.0 41.0 33.0 41.0 20-21 37.47527758575074 40.0 37.5 41.0 33.0 41.0 22-23 37.286711302284694 40.0 36.5 41.0 33.0 41.0 24-25 37.06178149883503 40.0 36.0 41.0 33.0 41.0 26-27 36.742026610680114 40.0 35.0 41.0 32.0 41.0 28-29 36.37163205912726 39.5 35.0 41.0 31.5 41.0 30-31 35.61174053306705 37.5 35.0 40.5 31.0 41.0 32-33 34.74210555512883 36.0 35.0 40.0 30.0 41.0 34-35 34.211261745653786 35.0 35.0 40.0 28.0 41.0 36-37 33.72773553182955 35.0 35.0 40.0 24.0 41.0 38-39 33.17036212031987 35.0 35.0 39.0 20.5 41.0 40-41 32.38832134231167 35.0 33.0 39.0 8.0 41.0 42-43 31.557132310896044 35.0 33.0 39.0 2.0 41.0 44-45 30.47861458893412 35.0 32.0 39.0 2.0 41.0 46-47 29.137391077997115 35.0 27.5 38.0 2.0 41.0 48-49 28.020734652772447 35.0 22.5 38.0 2.0 40.5 50-51 26.969952035913323 34.0 19.0 37.5 2.0 39.5 52-53 26.857347381177938 35.0 12.5 37.0 2.0 40.0 54-55 26.71154765257615 35.0 4.0 37.0 2.0 40.0 56-57 26.24194126533016 35.0 2.0 36.5 2.0 40.0 58-59 25.847451587850237 35.0 2.0 36.0 2.0 40.0 60-61 25.38429304179362 35.0 2.0 35.5 2.0 40.0 62-63 24.869786465934403 34.0 2.0 35.0 2.0 39.5 64-65 24.396179515409102 33.5 2.0 35.0 2.0 39.0 66-67 23.87439084756467 33.0 2.0 35.0 2.0 39.0 68-69 23.327246076247533 33.0 2.0 35.0 2.0 38.0 70-71 22.847329970726545 33.0 2.0 35.0 2.0 37.5 72-73 22.333612839354448 32.0 2.0 35.0 2.0 37.0 74-75 21.739013919826576 31.0 2.0 35.0 2.0 36.0 76-77 21.15902612421162 30.5 2.0 35.0 2.0 35.5 78-79 20.569867970743616 29.5 2.0 35.0 2.0 35.0 80-81 19.977808075377013 29.0 2.0 35.0 2.0 35.0 82-83 19.373063727372816 27.0 2.0 35.0 2.0 35.0 84-85 18.740296233709707 25.5 2.0 34.5 2.0 35.0 86-87 18.131529986088708 23.5 2.0 34.0 2.0 35.0 88-89 17.467511158904507 19.0 2.0 34.0 2.0 35.0 90-91 16.79056037756783 4.5 2.0 33.5 2.0 35.0 92-93 16.090218569441245 2.0 2.0 33.0 2.0 35.0 94-95 15.390755818419233 2.0 2.0 33.0 2.0 35.0 96-97 14.592872809824957 2.0 2.0 33.0 2.0 35.0 98-99 13.711016377772658 2.0 2.0 32.5 2.0 35.0 100-101 11.580465729574723 2.0 2.0 27.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4327770649882723 1101 2 0.1499677294966162 1101 3 0.2780441958353954 1101 4 -0.1762501769727436 1101 5 0.16390979365483815 1101 6 -0.17888835561279848 1101 7 0.07230042838826023 1101 8 0.13296020165620348 1101 9 0.20015507146282374 1101 10-11 0.19630699215195335 1101 12-13 0.27313064181058166 1101 14-15 0.2875946411942465 1101 16-17 0.25011715837189996 1101 18-19 0.24948257815363206 1101 20-21 0.12675731953986968 1101 22-23 0.3459853945051279 1101 24-25 0.3611307624852387 1101 26-27 0.6132857927625182 1101 28-29 0.5181926072644742 1101 30-31 0.7722489409914814 1101 32-33 0.4210778646355493 1101 34-35 0.7540334759852101 1101 36-37 0.10402849394601787 1101 38-39 0.6460749821837837 1101 40-41 0.7062478499346696 1101 42-43 0.49757257731018356 1101 44-45 0.5956440113833104 1101 46-47 0.34481380705727105 1101 48-49 0.06492955254330823 1101 50-51 -0.5977543569658437 1101 52-53 -0.7208008462707909 1101 54-55 -0.21360274320365846 1101 56-57 0.12062253763907904 1101 58-59 -0.09345284874927273 1101 60-61 -0.14711224697314762 1101 62-63 -0.8514090355627566 1101 64-65 -0.8579273697110068 1101 66-67 -0.6362068783186672 1101 68-69 -0.2231765842507265 1101 70-71 -0.3912324469894166 1101 72-73 -0.3525712222119459 1101 74-75 -0.4016939555517922 1101 76-77 -0.44925481373303455 1101 78-79 -0.6633783331771639 1101 80-81 -0.4410846037074059 1101 82-83 0.23116641172742902 1101 84-85 0.5653270569619799 1101 86-87 0.7165543647511257 1101 88-89 0.514916188547474 1101 90-91 0.2092955577694191 1101 92-93 0.4177219962090035 1101 94-95 0.3704094122464934 1101 96-97 0.5701229559230825 1101 98-99 0.6183875077843393 1101 100-101 0.7250345585717444 1102 1 -0.24800357513662874 1102 2 -0.40978246566341525 1102 3 -0.14809163554633642 1102 4 -0.5157349544903127 1102 5 -0.6036468027542199 1102 6 -0.47372051798594583 1102 7 -0.43470971993336605 1102 8 -0.4744090411228754 1102 9 -0.35619153275263216 1102 10-11 -0.296807761946404 1102 12-13 -0.06074913961018069 1102 14-15 0.05783663963297414 1102 16-17 -0.21956277917688993 1102 18-19 0.015696473547855305 1102 20-21 -0.10292261800892533 1102 22-23 0.1262351993451034 1102 24-25 0.08840632844932372 1102 26-27 0.19900007847680712 1102 28-29 0.3208623964916484 1102 30-31 0.2309062399766404 1102 32-33 0.09364617064647263 1102 34-35 -0.13780102830830998 1102 36-37 0.06927439558536008 1102 38-39 0.39324125853038083 1102 40-41 0.21986221371296466 1102 42-43 0.6095632096286927 1102 44-45 0.7338485390960336 1102 46-47 0.8794586158004414 1102 48-49 -0.009621579384873513 1102 50-51 -0.1794561524381315 1102 52-53 -0.5189116971685266 1102 54-55 -0.5603630866853138 1102 56-57 -0.4005952609557717 1102 58-59 -0.8147174857516148 1102 60-61 -0.9222722781987507 1102 62-63 -1.0785753119093577 1102 64-65 -0.8296369715845451 1102 66-67 -0.7022334200829121 1102 68-69 -0.9496246716824182 1102 70-71 -0.563004500072946 1102 72-73 -0.7354752034765788 1102 74-75 -0.46564399458380024 1102 76-77 -0.39184653894771415 1102 78-79 -0.20848371959402456 1102 80-81 -0.05241949832099024 1102 82-83 0.36882449135271855 1102 84-85 0.7124933333085828 1102 86-87 0.7334318042515129 1102 88-89 0.6030504586489585 1102 90-91 0.6442994609700428 1102 92-93 0.5129288658421025 1102 94-95 0.5234304895298667 1102 96-97 0.7837685453063763 1102 98-99 1.0968886787445253 1102 100-101 1.00931246645621 1103 1 0.6046843200368883 1103 2 0.38102232934702585 1103 3 0.372464539889247 1103 4 0.2569884168941172 1103 5 0.32136678692334897 1103 6 0.4498625793161466 1103 7 0.46200873055729375 1103 8 0.12616887779682173 1103 9 0.37490451050545914 1103 10-11 0.46342741100011864 1103 12-13 0.5993086522817777 1103 14-15 0.6745505125480307 1103 16-17 0.8317775921789305 1103 18-19 0.8198191525739773 1103 20-21 0.9196219418285736 1103 22-23 1.0138986330989965 1103 24-25 1.2416692815577832 1103 26-27 1.1597330627699947 1103 28-29 1.2945127269353875 1103 30-31 0.9888383201986599 1103 32-33 0.7020202730125149 1103 34-35 0.5215129075484128 1103 36-37 0.422248389233971 1103 38-39 0.47834124994742666 1103 40-41 0.449613594138107 1103 42-43 0.24620384133412188 1103 44-45 0.3081197032307337 1103 46-47 -0.4395840987018893 1103 48-49 -0.47059774738189475 1103 50-51 -0.8040520383420606 1103 52-53 -0.6009504349020531 1103 54-55 -0.6791225636972982 1103 56-57 -0.603281725636915 1103 58-59 -0.29755157724590475 1103 60-61 0.12928266701338842 1103 62-63 0.4017697228516077 1103 64-65 0.04183328847897272 1103 66-67 0.004286764164504575 1103 68-69 0.10205901784351568 1103 70-71 0.10945566071439572 1103 72-73 -0.604628065891827 1103 74-75 -0.6446558104508249 1103 76-77 -0.3156871248773889 1103 78-79 -0.6346273982482167 1103 80-81 -0.7591399515009769 1103 82-83 -0.714473079671226 1103 84-85 -0.8280611255361485 1103 86-87 -0.9605286569392284 1103 88-89 -0.4654129064413084 1103 90-91 0.10830827280307531 1103 92-93 0.6289561024169288 1103 94-95 0.5903346179308624 1103 96-97 0.6784700015476144 1103 98-99 0.4279761390483632 1103 100-101 0.13626866477967248 1104 1 -0.6200800778688702 1104 2 -0.7193179847891003 1104 3 -0.6098129470217479 1104 4 -0.6569644626870357 1104 5 -0.8739473492023038 1104 6 -0.6381740698985183 1104 7 -0.5205567144688814 1104 8 -0.43061122691522513 1104 9 -0.5212734999657442 1104 10-11 -0.6361930078480427 1104 12-13 -0.5343693581894229 1104 14-15 -0.19811964452004105 1104 16-17 -0.23381141305667086 1104 18-19 -0.4808745647035124 1104 20-21 -0.5496712518887037 1104 22-23 -0.4658003197805769 1104 24-25 -0.514940666086197 1104 26-27 -0.6759999215231929 1104 28-29 -0.5407359641640923 1104 30-31 -0.4834653447228092 1104 32-33 -0.4914221353644521 1104 34-35 -0.5541808097290755 1104 36-37 -0.9059715060539872 1104 38-39 -0.8674964463876549 1104 40-41 -0.48732357863676157 1104 42-43 -0.4056417084040973 1104 44-45 -0.470427417188116 1104 46-47 -0.39768619294272867 1104 48-49 -0.480427590313834 1104 50-51 -0.36918292839441946 1104 52-53 -0.5725865605565019 1104 54-55 -0.7382456003465165 1104 56-57 -0.37473460521806246 1104 58-59 -0.302381420177845 1104 60-61 -0.0013979612588634893 1104 62-63 0.12716239300867116 1104 64-65 0.5217154874318517 1104 66-67 1.0884359788241902 1104 68-69 1.0193234157492732 1104 70-71 1.1169818387248682 1104 72-73 1.0285002063594852 1104 74-75 1.1654489015910627 1104 76-77 1.1614594719812494 1104 78-79 1.427335952537124 1104 80-81 1.6496296820068785 1104 82-83 1.7600949831560015 1104 84-85 1.7692556283905496 1104 86-87 1.6151257933225551 1104 88-89 1.980987617118906 1104 90-91 1.9875098434837035 1104 92-93 1.7155791390661435 1104 94-95 1.5332665551036389 1104 96-97 1.317265813065939 1104 98-99 0.961601793498625 1104 100-101 1.0953917014288859 1105 1 0.5961584542412233 1105 2 0.618617795027415 1105 3 0.3753705392167852 1105 4 0.3149162712579212 1105 5 0.1599517333664977 1105 6 0.15251400349598043 1105 7 0.3265075057146021 1105 8 0.62126950702973 1105 9 0.7523962248048974 1105 10-11 0.5768574508675499 1105 12-13 0.5685434858472718 1105 14-15 0.7405959518102421 1105 16-17 0.3507986786864521 1105 18-19 0.18488231603043204 1105 20-21 0.33670489490028643 1105 22-23 0.4092226159992336 1105 24-25 0.46430245317986163 1105 26-27 0.3824863170089188 1105 28-29 0.495663118404714 1105 30-31 0.4549750222496698 1105 32-33 0.4815365802318823 1105 34-35 0.7466678141241374 1105 36-37 1.0555881269735394 1105 38-39 1.1709897921444608 1105 40-41 1.313547980996269 1105 42-43 1.15167480535737 1105 44-45 1.5116597387751902 1105 46-47 1.5862816969655285 1105 48-49 1.612439382163231 1105 50-51 2.271275787201912 1105 52-53 2.130784999076525 1105 54-55 2.364575500570915 1105 56-57 2.3583158534975333 1105 58-59 2.0653020660606884 1105 60-61 2.5402534148879283 1105 62-63 2.072575237045374 1105 64-65 2.0552705333034105 1105 66-67 2.036348822860891 1105 68-69 1.768841764373125 1105 70-71 1.8572341323028496 1105 72-73 2.0438901146163637 1105 74-75 1.429026883242436 1105 76-77 1.5548998389537267 1105 78-79 1.5267396222618927 1105 80-81 1.3177489480619258 1105 82-83 1.9150720473761815 1105 84-85 2.1416180137116534 1105 86-87 2.2064101969922767 1105 88-89 2.3044967914308288 1105 90-91 2.252899751740582 1105 92-93 2.268744276680824 1105 94-95 2.3529913257458386 1105 96-97 2.450385079120986 1105 98-99 2.224652252214222 1105 100-101 2.344864178493106 1106 1 0.5202972806216977 1106 2 0.44945560011656127 1106 3 0.2542436567518358 1106 4 0.1995449712752304 1106 5 0.37727906589203286 1106 6 0.3504108357618634 1106 7 0.4249149836443209 1106 8 0.46344537685835974 1106 9 0.6175944245625544 1106 10-11 0.5035711430953498 1106 12-13 0.6179419625652969 1106 14-15 0.7064086573667581 1106 16-17 0.6370848133584204 1106 18-19 0.7766254352964879 1106 20-21 0.8598570499980909 1106 22-23 0.5562279821062148 1106 24-25 0.33246499429116483 1106 26-27 0.4466415879107686 1106 28-29 0.5573300735717552 1106 30-31 1.076534655277193 1106 32-33 1.3886250344468678 1106 34-35 0.9358663600822439 1106 36-37 1.0024247203611054 1106 38-39 1.192692232857631 1106 40-41 0.9162613270236193 1106 42-43 1.3034620651808133 1106 44-45 0.7617895639439602 1106 46-47 0.5842477693214256 1106 48-49 0.7970724096861659 1106 50-51 1.2161000904735069 1106 52-53 1.1037813639717982 1106 54-55 0.9061411921063147 1106 56-57 0.8487087910083524 1106 58-59 1.2224770703881944 1106 60-61 1.4501114727034015 1106 62-63 1.2398982420652764 1106 64-65 1.1665739779978885 1106 66-67 1.1565020165600401 1106 68-69 1.1322007742398377 1106 70-71 1.1983497632531694 1106 72-73 1.4439247346613726 1106 74-75 1.4019583355533243 1106 76-77 1.2141953210378524 1106 78-79 1.4734680280088206 1106 80-81 1.1831202480446166 1106 82-83 1.2185383793824158 1106 84-85 1.1157178925414932 1106 86-87 1.2052201329451933 1106 88-89 1.209053654854749 1106 90-91 1.292085315181815 1106 92-93 1.3762395164246364 1106 94-95 1.145530706047035 1106 96-97 1.0185865677829202 1106 98-99 1.012215001045794 1106 100-101 1.296287927843979 1107 1 0.07074941521868894 1107 2 0.09798616267633875 1107 3 -0.04527377651483633 1107 4 -0.05717183595891839 1107 5 -0.22632062109170903 1107 6 -0.3344643924791626 1107 7 0.047599967558767275 1107 8 -0.04805362322858997 1107 9 0.09527027883609662 1107 10-11 -0.007656141488602941 1107 12-13 -0.04438087892674503 1107 14-15 0.18280201446614086 1107 16-17 0.162006559293566 1107 18-19 0.15104939843012488 1107 20-21 0.17956837944770143 1107 22-23 0.009579864551220396 1107 24-25 0.1767067993515994 1107 26-27 8.433964491558754E-4 1107 28-29 -0.09222213927929346 1107 30-31 0.10533649859516458 1107 32-33 0.0266999844512128 1107 34-35 0.09007034234466005 1107 36-37 -0.03716966273600519 1107 38-39 -0.027473404912996102 1107 40-41 -0.20813003024966292 1107 42-43 -0.5336716623211508 1107 44-45 -0.13348041257982501 1107 46-47 0.01605804668860955 1107 48-49 0.11101250185206624 1107 50-51 0.6090659195318509 1107 52-53 0.7968950062637745 1107 54-55 0.7822497913585522 1107 56-57 0.6931570998510601 1107 58-59 1.0167775659972733 1107 60-61 0.7613670156996655 1107 62-63 0.6739365865570583 1107 64-65 0.6037316164641062 1107 66-67 0.4303369004831765 1107 68-69 0.6129524480073378 1107 70-71 0.5222237742087401 1107 72-73 0.6266914505991146 1107 74-75 0.8497346158767769 1107 76-77 0.7294548636863176 1107 78-79 0.636345169126983 1107 80-81 0.6863808340806123 1107 82-83 0.6084936006675221 1107 84-85 -0.01467294303801836 1107 86-87 0.11673869654836011 1107 88-89 0.15685167241844056 1107 90-91 -0.04563531780661734 1107 92-93 0.3588279869924129 1107 94-95 -0.004521463329542286 1107 96-97 0.573625260070548 1107 98-99 0.8935948810562273 1107 100-101 0.8638364018897153 1108 1 0.628353101854632 1108 2 0.7062810935519117 1108 3 0.4570303709506014 1108 4 0.5234733253314019 1108 5 0.5386563374336362 1108 6 0.4212959761844317 1108 7 0.4807796910610733 1108 8 0.5141583583382356 1108 9 0.8786804170849436 1108 10-11 0.6651549184192334 1108 12-13 0.7666790289073475 1108 14-15 1.030728281747244 1108 16-17 1.2495641629801995 1108 18-19 0.9436761265407299 1108 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72-73 -0.08287829831341398 2316 74-75 0.08093176763467369 2316 76-77 0.6172694408286041 2316 78-79 0.6628967002006725 2316 80-81 0.8448789031906827 2316 82-83 0.8927507463958761 2316 84-85 0.8511481517550372 2316 86-87 0.9875556998646076 2316 88-89 1.6783785828509892 2316 90-91 2.162735700181525 2316 92-93 2.1240059303434045 2316 94-95 1.9831107918637603 2316 96-97 2.3910664361188996 2316 98-99 1.8974351268319563 2316 100-101 1.8104462185628432 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4791.0 3 784.0 4 558.0 5 470.0 6 490.0 7 558.0 8 628.0 9 749.0 10 946.0 11 1527.0 12 2440.0 13 3468.0 14 4346.0 15 6454.0 16 8131.0 17 10732.0 18 9291.0 19 4861.0 20 4525.0 21 4201.0 22 4199.0 23 4320.0 24 4750.0 25 4873.0 26 5246.0 27 5698.0 28 6173.0 29 6964.0 30 7630.0 31 8609.0 32 9770.0 33 11773.0 34 16672.0 35 30956.0 36 15948.0 37 12224.0 38 7967.0 39 620.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.49375851403799 21.202203582964213 15.54117153162724 11.762866371370556 2 17.206159782154046 26.401871153337257 38.784177144954626 17.607791919554064 3 19.438740880819946 36.240656709027185 31.436145462927783 12.884456947225079 4 10.800220255855349 23.536216294664772 34.837988159113515 30.82557529036637 5 8.9195524000222 43.714430872615026 28.938166821075285 18.42784990628749 6 21.110247717937987 41.10017163497255 17.25956160500047 20.53001904208899 7 19.98523153621902 46.45407477281749 18.121673360849915 15.439020330113582 8 27.72544316062198 31.84097864548431 17.521117622704168 22.912460571189545 9 31.066937569895593 17.8429045834436 19.46747027036256 31.62268757629824 10-11 23.82385330499142 33.21638025968704 20.74743258124824 22.212333854073297 12-13 29.351468096276722 34.101065805030665 23.854697097100516 12.692769001592092 14-15 24.707790475580737 31.522989425611147 30.78737048774502 12.981849611063096 16-17 14.732578265221411 35.9878096408838 29.929551163440877 19.350060930453918 18-19 13.807681391075723 29.102173129002722 42.68577660071662 14.40436887920493 20-21 17.833237998480335 26.785821003833316 43.485072269510205 11.895868728176143 22-23 16.540400268040443 28.873675269854417 43.0219852919208 11.563939170184343 24-25 14.390491722094623 29.860441089831706 44.01185973240892 11.737207455664752 26-27 13.586613164859557 30.55237769999146 44.183812857508755 11.677196277640228 28-29 13.188278982414205 31.301007341642478 43.80207444084002 11.708639235103295 30-31 13.330530216039238 31.90708733346709 43.18202191553937 11.580360534954304 32-33 12.154429639494852 32.42582762940814 43.33339736242816 12.086345368668857 34-35 12.265121441072267 32.48196525376702 43.27570751696761 11.97720578819311 36-37 12.699154613343918 32.40214878890518 42.26399711445908 12.634699483291822 38-39 12.512536863714155 32.99531814825721 42.019726088199775 12.47241889982886 40-41 12.170931197760295 33.171586294605795 41.90101849421616 12.756464013417748 42-43 12.095340723590855 33.21006753656897 41.23230232483703 13.462289415003148 44-45 12.652461143213184 32.99412773875265 40.46077619685732 13.89263492117684 46-47 12.730333985612862 33.572905574535916 39.30172658462313 14.395033855228087 48-49 12.817093156250534 33.451194113931614 37.89796684818792 15.833745881629934 50-51 13.25213539343944 33.90874620451553 36.49298302140835 16.34613538063668 52-53 13.610487594629886 34.226787406010224 35.46135003883346 16.701374960526426 54-55 13.653772705256802 33.455381089852324 35.04861640091408 17.842229803976796 56-57 14.481317973195862 33.37842653187072 34.0691206186161 18.07113487631732 58-59 14.707112345789646 32.75959187331174 34.469081117526315 18.064214663372294 60-61 15.278026714462511 32.939871121922074 34.529723040157045 17.25237912345837 62-63 15.86918532079554 32.63154974896674 34.69699976315604 16.802265167081675 64-65 16.09094109739574 32.879623603717015 34.70463347238374 16.324801826503503 66-67 16.259668213580838 32.52531071638129 34.35002933802742 16.864991732010452 68-69 16.34667497407491 32.03679442151846 34.79338878243837 16.823141821968257 70-71 16.21573175650155 32.11180084460937 34.899566598878835 16.772900800010245 72-73 15.999257406727724 31.37541930913219 35.237544491579676 17.38777879256041 74-75 15.076198239182666 31.870751057301245 35.34309478796362 17.709955915552463 76-77 14.407188337515578 31.313480480019116 35.93955378023591 18.339777402229394 78-79 14.327596434103896 31.08124831968455 35.934212716200584 18.656942530010966 80-81 14.619527412547423 30.63432724355281 35.57805971996398 19.16808562393578 82-83 14.788110276544897 30.464322294298395 34.70318368043701 20.0443837487197 84-85 15.539178137142152 29.814132422380922 34.51128413137686 20.135405309100065 86-87 15.517918256223808 29.770378335936364 34.30563023273677 20.406073175103053 88-89 16.02720461291899 29.384951248087376 34.231119704138095 20.356724434855536 90-91 16.555271020059752 29.066794707639776 34.04310712761417 20.334827144686297 92-93 17.16943890234404 28.703575277134657 34.238799918913436 19.888185901607862 94-95 17.366428504357774 28.168668425141064 34.47271376987887 19.992189300622297 96-97 17.83026961045834 27.847512387911276 34.159265218671706 20.162952782958673 98-99 18.22680597167806 27.769455327648462 33.533072136436964 20.470666564236513 100-101 19.690552319682865 26.620446016459166 34.02877897601183 19.660222687846147 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7558.0 1 4699.0 2 2118.5 3 2278.5 4 1671.0 5 1498.5 6 2705.5 7 5269.5 8 7670.0 9 7776.5 10 5210.0 11 2855.0 12 2421.0 13 2340.0 14 2196.0 15 1963.5 16 1684.5 17 1516.5 18 1501.5 19 1479.0 20 1468.0 21 1493.5 22 1583.0 23 1701.0 24 1808.0 25 2139.5 26 2552.5 27 2968.5 28 3252.5 29 3504.0 30 3944.5 31 4349.5 32 4846.0 33 5392.0 34 5923.0 35 6168.0 36 6091.0 37 6192.0 38 5832.5 39 5169.0 40 4848.0 41 4697.5 42 4677.0 43 4673.0 44 4671.5 45 4536.0 46 4328.0 47 4464.5 48 5030.5 49 5115.5 50 4716.5 51 4295.5 52 3755.5 53 3325.0 54 2994.0 55 2851.0 56 2906.5 57 2942.0 58 2830.5 59 2820.5 60 2953.0 61 3132.5 62 2799.5 63 1655.5 64 783.5 65 513.0 66 409.5 67 343.5 68 301.5 69 291.0 70 255.0 71 222.5 72 225.0 73 214.5 74 209.0 75 173.5 76 110.5 77 73.5 78 56.5 79 40.5 80 28.0 81 18.5 82 9.0 83 10.5 84 10.5 85 6.5 86 3.5 87 1.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38576098181290597 2 0.0204828839900658 3 0.03541832023282212 4 0.02944414573571959 5 0.04907357622619932 6 0.05291411697433665 7 0.025176878237789215 8 0.025176878237789215 9 0.0273105119867544 10-11 0.02560360498758225 12-13 0.025176878237789215 14-15 0.021122974114755357 16-17 0.024963514862892694 18-19 0.020696247364962318 20-21 0.034138139983443005 22-23 0.02090961073985884 24-25 0.044379581978475906 26-27 0.034991593483029076 28-29 0.026457058487168325 30-31 0.03371141323364996 32-33 0.031364416109788254 34-35 0.030724325985098704 36-37 0.030084235860409148 38-39 0.014081982743170237 40-41 0.010881532119722457 42-43 0.01045480536992942 44-45 0.008534534995860751 46-47 0.015148799617652833 48-49 0.011094895494618976 50-51 0.0074677181213781575 52-53 0.0034138139983443 54-55 0.0032004506234477813 56-57 0.00533408437241297 58-59 0.0012801802493791125 60-61 0.00512072099751645 62-63 0.004480630872826894 64-65 0.006187537871999044 66-67 0.001920270374068669 68-69 0.005547447747309488 70-71 0.01386861936827372 72-73 0.011948348994205051 74-75 0.008107808246067714 76-77 0.007681081496274676 78-79 0.004693994247723413 80-81 0.006400901246895563 82-83 0.009387988495446826 84-85 0.0036271773732408186 86-87 0.0 88-89 0.018562613615997134 90-91 0.017922523491307577 92-93 0.01045480536992942 94-95 0.0204828839900658 96-97 0.017495796741514538 98-99 0.015362162992549352 100-101 0.10561487057377679 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 234342.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.90542028317587 #Duplication Level Percentage of deduplicated Percentage of total 1 80.73358994725159 33.8317501770916 2 9.801226044276085 8.214489933515972 3 3.6394370786745687 4.575364211280948 4 1.6608623042300565 2.783965315649777 5 0.9582289566403943 2.0077493577762415 6 0.5967291908515101 1.5003712522723198 7 0.4419461925419034 1.296395865871248 8 0.2810533390358649 0.9422126635430269 9 0.23421111586322071 0.8833243720715876 >10 1.3869371295900288 11.199016821568476 >50 0.15274637991079612 4.472096337831033 >100 0.09877599234231482 7.19290609451144 >500 0.0040732367976212295 1.0168898447568084 >1k 0.006109855196431844 4.713623678213893 >5k 0.003054927598215922 10.427068131192872 >10k+ 0.0010183091994053074 4.942775942852753 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11583 4.942775942852753 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9343 3.98690802331635 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8571 3.6574749724761246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6521 2.782685135400398 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2815 1.2012358006674007 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2297 0.9801913442746071 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1818 0.7757892311237422 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1680 0.7169009396523031 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1279 0.545783512985295 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1157 0.49372284951054446 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 729 0.3110838005991243 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 625 0.2667042186206484 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 519 0.22147118314258646 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 510 0.21763064239444913 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 434 0.1851994094101783 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 403 0.17197088016659412 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 399 0.17026397316742195 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 395 0.16855706616824984 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 377 0.16087598467197517 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 376 0.1604492579221821 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 338 0.1442336414300467 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 331 0.14124655418149543 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 330 0.14081982743170238 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 328 0.1399663739321163 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 302 0.12887147843749733 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 293 0.12503093768936 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 290 0.12375075743998089 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 289 0.12332403069018785 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 287 0.12247057719060175 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 255 0.10881532119722456 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 252 0.10753514094784546 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 250 0.1066816874482594 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 237 0.1011342397009499 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 235 0.10028078620136383 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.2672674979303755E-4 0.0 14-15 0.0 0.0 0.0 4.2672674979303755E-4 0.0 16-17 0.0 0.0 0.0 0.001066816874482594 0.0 18-19 0.0 0.0 0.0 0.0012801802493791125 0.0 20-21 0.0 0.0 0.0 0.0032004506234477813 0.0 22-23 0.0 0.0 0.0 0.012375075743998088 0.0 24-25 0.0 0.0 0.0 0.04075240460523508 0.0 26-27 0.0 0.0 0.0 0.09516006520384737 0.0 28-29 0.0 0.0 0.0 0.1851994094101783 0.0 30-31 0.0 0.0 0.0 0.38256053118945815 0.0 32-33 0.0 0.0 0.0 0.6038183509571482 0.0 34-35 0.0 0.0 0.0 0.8540935897107647 0.0 36-37 0.0 0.0 0.0 1.1621903030613376 0.0 38-39 0.0 0.0 0.0 1.5564858198701044 0.0 40-41 0.0 0.0 0.0 1.9761715782915568 0.0 42-43 0.0 0.0 0.0 2.2919493731384044 0.0 44-45 0.0 0.0 0.0 2.605806897611184 0.0 46-47 0.0 0.0 0.0 2.939507215949339 0.0 48-49 0.0 0.0 0.0 3.2926236014030774 0.0 50-51 0.0 0.0 0.0 3.655127975352263 0.0 52-53 0.0 0.0 0.0 3.954263426957182 0.0 54-55 0.0 0.0 0.0 4.2457177970658275 0.0 56-57 0.0 0.0 0.0 4.555308054040676 0.0 58-59 0.0 0.0 0.0 4.854016778895802 0.0 60-61 0.0 0.0 0.0 5.19923871947837 0.0 62-63 0.0 0.0 0.0 5.500507804832254 0.0 64-65 0.0 0.0 0.0 5.782787549820348 0.0 66-67 0.0 0.0 0.0 6.107739969787746 0.0 68-69 0.0 0.0 0.0 6.4489080062472794 0.0 70-71 0.0 0.0 0.0 6.806931749323638 0.0 72-73 0.0 0.0 0.0 7.119935820296831 0.0 74-75 0.0 0.0 0.0 7.450008961261746 0.0 76-77 0.0 0.0 0.0 7.751704773365423 0.0 78-79 0.0 0.0 0.0 8.098846984322059 0.0 80-81 0.0 0.0 0.0 8.452816823275384 0.0 82-83 0.0 0.0 0.0 8.785450324739056 0.0 84-85 0.0 0.0 0.0 9.097387578837766 0.0 86-87 0.0 0.0 0.0 9.402497204939788 0.0 88-89 0.0 0.0 0.0 9.704833107168156 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 120 0.0 67.5593 1 GTCACTA 145 0.0 58.97254 2 AGATAGT 25 0.005013934 57.00679 6 GTACAGG 45 1.4770971E-5 52.98769 1 GGTATCA 3455 0.0 52.45091 1 TCACTAC 175 0.0 48.86296 3 ACTACCT 205 0.0 41.712284 5 GTATCAA 5940 0.0 39.981617 1 TGTCCAG 50 0.0016122458 38.004528 5 CACTACC 230 0.0 37.17834 4 GTATATG 80 1.0451124E-5 35.76669 1 ATATGGG 55 0.0025739474 34.54957 3 CCTCTAG 60 0.0038686479 31.792612 1 CATGGAT 105 1.8913779E-6 31.670437 4 GTCTACC 30 0.0039545395 31.65692 48-49 TATCAAC 7590 0.0 31.04454 2 ATCAACG 7930 0.0 29.773407 3 TCAACGC 8005 0.0 29.553802 4 GGGAGCA 65 0.0058313045 29.234253 9 CAACGCA 8160 0.0 28.934204 5 >>END_MODULE