FastQCFastQC Report
Thu 26 May 2016
SRR938275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234342
Sequences flagged as poor quality0
Sequence length101
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108214.617610159510459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95694.0833482687695755No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82573.523482773041111No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66032.8176767288834266No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26421.1274120729532051No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24401.0412132694950116No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT23430.9998207747650869No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18400.7851772196191891No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16660.7109267651552005No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT14570.6217408744484557RNA PCR Primer, Index 23 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.5026841112561982No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11190.477507233018409No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT7690.32815287059084586RNA PCR Primer, Index 23 (96% over 25bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.3140708878476756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6320.2696913058691997No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5310.2265919041401029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5150.21976427614341432No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5000.2133633748965188No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.1967210316545903No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT4050.1728243336661802No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.16855706616824984No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3730.159169077672803No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.15831562417321693No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT3540.15106126942673528RNA PCR Primer, Index 23 (96% over 28bp)
CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA3530.15063454267694223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3460.14764745542839097No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC3410.1455138216794258No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG3350.14295346118066757No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC3180.13569910643418595No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.12887147843749733No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG2990.1275912981881182RNA PCR Primer, Index 23 (96% over 30bp)
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT2810.11991021669184355No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT2800.11948348994205052No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA2780.11863003644246442No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2780.11863003644246442No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2680.11436276894453407No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.11094895494618975No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG2540.10838859444743153Illumina PCR Primer Index 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAT200.00205900171.363192
TTCATGC250.005018710656.9930848
GGGATAC250.005018710656.9930849
GGTATCA39750.047.5156141
TACTAGA205.382448E-447.5043820-21
CGTACTA205.3881155E-447.49423618-19
ACCGTAC205.3881155E-447.49423616-17
GAGAGTG500.001614152837.995399
CGTCACT1054.566209E-836.247971
GTATCAA62200.034.572521
ATTATAT1051.8698138E-631.7169723
CTAGATC300.003946803531.66958422-23
ATGGGGG4550.031.3684355
CGCAGAA650.005838164629.2272249
GGGCAAA504.9438277E-528.50262520-21
CTTGGTA504.9510276E-528.49654216-17
TGGGAGT1004.9406073E-528.4965426
TATCAAC77850.027.622492
CATGGGG11550.027.1859764
GACTATT350.00839155427.13956336-37
Produced by FastQC (version 0.11.5)