##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938274_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 233200 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.320197255574612 33.0 31.0 34.0 30.0 34.0 2 31.36774013722127 33.0 31.0 34.0 30.0 34.0 3 31.287234133790736 33.0 31.0 34.0 28.0 34.0 4 34.8648627787307 37.0 35.0 37.0 33.0 37.0 5 34.93514579759863 37.0 35.0 37.0 33.0 37.0 6 35.08126929674099 37.0 35.0 37.0 33.0 37.0 7 35.04578044596912 37.0 35.0 37.0 33.0 37.0 8 35.03623070325901 37.0 35.0 37.0 33.0 37.0 9 36.52522727272727 39.0 37.0 39.0 33.0 39.0 10-11 36.49299313893654 39.0 37.0 39.0 33.0 39.0 12-13 36.35081260720412 39.0 37.0 39.0 32.0 39.0 14-15 37.13082547169812 40.0 37.5 41.0 32.0 41.0 16-17 37.07764365351629 40.0 37.5 41.0 32.0 41.0 18-19 37.35726629502573 40.0 37.5 41.0 33.0 41.0 20-21 37.44295454545454 40.0 37.5 41.0 33.0 41.0 22-23 37.25882504288165 40.0 36.5 41.0 33.0 41.0 24-25 37.0497384219554 40.0 36.0 41.0 32.5 41.0 26-27 36.731760291595194 40.0 35.0 41.0 32.0 41.0 28-29 36.35064536878216 39.5 35.0 41.0 31.5 41.0 30-31 35.606852487135505 38.0 35.0 40.5 31.0 41.0 32-33 34.757819468267584 36.0 35.0 40.0 30.0 41.0 34-35 34.25736492281304 35.0 35.0 40.0 28.0 41.0 36-37 33.75714837049743 35.0 35.0 40.0 24.0 41.0 38-39 33.220960548885074 35.0 35.0 39.5 20.0 41.0 40-41 32.51624785591767 35.0 34.0 39.0 8.5 41.0 42-43 31.593434819897084 35.0 33.0 39.0 2.0 41.0 44-45 30.56902444253859 35.0 32.0 39.0 2.0 41.0 46-47 29.268312607204116 35.0 28.0 39.0 2.0 41.0 48-49 28.154189536878214 35.0 22.5 38.0 2.0 41.0 50-51 27.1490180102916 34.5 20.0 37.5 2.0 40.0 52-53 27.07397512864494 35.0 14.5 38.0 2.0 40.0 54-55 26.954476843910808 35.0 4.5 38.0 2.0 40.0 56-57 26.55666166380789 35.0 2.0 37.0 2.0 40.0 58-59 26.206292881646654 35.0 2.0 37.0 2.0 40.0 60-61 25.79794382504288 35.0 2.0 36.0 2.0 40.0 62-63 25.29222126929674 35.0 2.0 35.5 2.0 40.0 64-65 24.819541166380787 34.0 2.0 35.0 2.0 39.5 66-67 24.36420240137221 33.5 2.0 35.0 2.0 39.0 68-69 23.83722770154374 33.0 2.0 35.0 2.0 39.0 70-71 23.504172384219554 33.0 2.0 35.0 2.0 38.0 72-73 23.065754716981132 33.0 2.0 35.0 2.0 37.5 74-75 22.557748713550602 32.5 2.0 35.0 2.0 37.0 76-77 22.09129502572899 32.0 2.0 35.0 2.0 36.0 78-79 21.540709691252147 31.0 2.0 35.0 2.0 35.5 80-81 21.017272727272726 30.5 2.0 35.0 2.0 35.0 82-83 20.444826329331047 29.5 2.0 35.0 2.0 35.0 84-85 19.95767795883362 29.0 2.0 35.0 2.0 35.0 86-87 19.403784305317323 27.5 2.0 35.0 2.0 35.0 88-89 18.7689987135506 25.5 2.0 34.0 2.0 35.0 90-91 18.140851200686107 24.0 2.0 34.0 2.0 35.0 92-93 17.454847770154373 19.0 2.0 34.0 2.0 35.0 94-95 16.786723842195542 7.0 2.0 33.0 2.0 35.0 96-97 16.015345197255577 2.0 2.0 33.0 2.0 35.0 98-99 15.123496998284734 2.0 2.0 33.0 2.0 35.0 100-101 12.719343910806174 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.009342734341640124 1101 2 -0.01912788362658091 1101 3 -0.08394805947434492 1101 4 -0.29903352026210683 1101 5 0.11649671018550833 1101 6 -0.303043002986783 1101 7 -0.4691206257030771 1101 8 -0.23216975950218455 1101 9 -0.2747157006728429 1101 10-11 -0.3463770380404654 1101 12-13 -0.6964343678267397 1101 14-15 -0.7117261323125135 1101 16-17 -0.44635940229921545 1101 18-19 -0.4310691599878069 1101 20-21 -0.09867853849380026 1101 22-23 -0.38841112067147776 1101 24-25 -0.3736967740440704 1101 26-27 -0.24429615821374284 1101 28-29 -0.2536845935723022 1101 30-31 -0.5416012102280305 1101 32-33 -0.548508378536944 1101 34-35 -0.44512355883214383 1101 36-37 -0.3896604996534947 1101 38-39 -0.06687269429059484 1101 40-41 -0.09711034821529552 1101 42-43 -0.6711429819392478 1101 44-45 -1.60430621379545 1101 46-47 -2.027844147004565 1101 48-49 -2.2097410067138767 1101 50-51 -2.3240187839261495 1101 52-53 -2.4176225317412445 1101 54-55 -1.832437167970923 1101 56-57 -1.3915264778353205 1101 58-59 -1.2088186489517376 1101 60-61 -1.490593416810004 1101 62-63 -1.2370964041826475 1101 64-65 -1.5067601771050612 1101 66-67 -1.425537070232938 1101 68-69 -1.7983351761590747 1101 70-71 -1.540673028321482 1101 72-73 -1.6586493480301918 1101 74-75 -1.4801105950227367 1101 76-77 -2.039251326350538 1101 78-79 -1.694789249201424 1101 80-81 -1.6275487903076709 1101 82-83 -1.2803643456516731 1101 84-85 -1.6573495047898348 1101 86-87 -1.4947599561178073 1101 88-89 -1.3118804272394655 1101 90-91 -1.7418141262132671 1101 92-93 -1.6091860809530854 1101 94-95 -0.9992328455055137 1101 96-97 0.015328464125245134 1101 98-99 0.3589035318661349 1101 100-101 0.5302192054153849 1102 1 0.5868038742380115 1102 2 0.5412923811978274 1102 3 -0.03193309113813214 1102 4 0.33231362999696046 1102 5 0.35259342866564936 1102 6 0.8466403591318112 1102 7 0.5223013662370519 1102 8 0.5903403153394962 1102 9 0.9489745699086214 1102 10-11 0.6869706879238393 1102 12-13 0.8520831911830484 1102 14-15 1.1504356408596195 1102 16-17 0.9558282776086671 1102 18-19 0.44591702308645864 1102 20-21 0.778710638247702 1102 22-23 0.7093724141586861 1102 24-25 0.685370583468881 1102 26-27 0.606912822787983 1102 28-29 0.47327280424001117 1102 30-31 0.6043256752066313 1102 32-33 0.8328675569840698 1102 34-35 1.0196288879151254 1102 36-37 1.3278553322405742 1102 38-39 1.1470737651221796 1102 40-41 1.2402385291594982 1102 42-43 1.5156014049346709 1102 44-45 1.4378929802172173 1102 46-47 1.699933630773213 1102 48-49 1.1846026893137562 1102 50-51 0.9864158735292392 1102 52-53 0.7321471861631892 1102 54-55 0.7823440640382842 1102 56-57 0.9778891813471802 1102 58-59 0.3629717943412949 1102 60-61 0.47659138457168737 1102 62-63 -0.19161569030815073 1102 64-65 -0.4111643221827812 1102 66-67 -0.6677506568765743 1102 68-69 -0.6000335066138796 1102 70-71 -0.59379622924261 1102 72-73 -0.9704081275350838 1102 74-75 -0.55732706019257 1102 76-77 -0.8797521899083947 1102 78-79 -0.7261363994604935 1102 80-81 -0.7331187384941984 1102 82-83 -1.1009745932049277 1102 84-85 -0.8801906101438952 1102 86-87 -0.4993224201362345 1102 88-89 -0.3038925170494835 1102 90-91 -0.49595920393347726 1102 92-93 0.13391984881087637 1102 94-95 0.5658650243851042 1102 96-97 0.48130428450521023 1102 98-99 0.34990813750804683 1102 100-101 0.505449487510953 1103 1 0.6950570200559234 1103 2 0.8943641798654802 1103 3 0.8847027341764466 1103 4 0.9664426702140858 1103 5 1.237925281614082 1103 6 1.1572744573306792 1103 7 0.9896095330270853 1103 8 1.026560399227975 1103 9 1.0201255691684281 1103 10-11 0.8224721683087424 1103 12-13 0.9817402353478641 1103 14-15 0.896210375623987 1103 16-17 0.9240771056372878 1103 18-19 1.2730975066788517 1103 20-21 1.2808849535696893 1103 22-23 1.2528587205983683 1103 24-25 1.4114222735749777 1103 26-27 1.4830847941672118 1103 28-29 1.5443312794435684 1103 30-31 1.6175257738989544 1103 32-33 1.693356700828133 1103 34-35 2.1020986633900876 1103 36-37 2.384942674949677 1103 38-39 2.700984448566551 1103 40-41 2.9082467946418546 1103 42-43 2.659214160917898 1103 44-45 2.859185849696612 1103 46-47 2.402314583154162 1103 48-49 2.2599018504289816 1103 50-51 2.156536771629412 1103 52-53 2.7188854047666915 1103 54-55 2.912602514568757 1103 56-57 2.819981458672615 1103 58-59 2.860427382794292 1103 60-61 2.670120868904281 1103 62-63 2.847228992642748 1103 64-65 3.0182398228949374 1103 66-67 2.5641454694496026 1103 68-69 2.666744188920294 1103 70-71 2.3327396700912146 1103 72-73 2.6225014456206033 1103 74-75 2.623064008151868 1103 76-77 2.652415340316132 1103 78-79 2.8778297984176255 1103 80-81 2.955784543025665 1103 82-83 2.807334067046739 1103 84-85 2.6027695428292112 1103 86-87 2.6449225835647283 1103 88-89 2.756571953712914 1103 90-91 3.071876349977206 1103 92-93 3.0560916968246943 1103 94-95 3.519417948145282 1103 96-97 3.5278284641252426 1103 98-99 4.221006706469309 1103 100-101 4.155616030812212 1104 1 0.21026866026756608 1104 2 0.37716841266971457 1104 3 0.32253342200713675 1104 4 0.25744796121937696 1104 5 0.1664967101855126 1104 6 -0.06230226224604252 1104 7 0.19199048540803432 1104 8 -0.15531790765033548 1104 9 0.08361763266049138 1104 10-11 0.2943637027002737 1104 12-13 -0.040415849308217844 1104 14-15 0.4229960899097094 1104 16-17 0.30039985696004123 1104 18-19 0.28004195112330166 1104 20-21 0.1985436837284169 1104 22-23 0.37686665710629796 1104 24-25 0.5165810037337053 1104 26-27 0.8177408788232938 1104 28-29 0.7893709619832521 1104 30-31 0.4898802712534547 1104 32-33 0.38713976961120267 1104 34-35 0.7039505152419352 1104 36-37 1.1987654262724305 1104 38-39 1.2201643427464361 1104 40-41 1.3788155777106397 1104 42-43 1.1876533143570498 1104 44-45 0.9091197121304795 1104 46-47 0.40826696410654506 1104 48-49 -0.009278043750914122 1104 50-51 -0.04253730244466425 1104 52-53 -0.5208632724819857 1104 54-55 -0.5893816124153695 1104 56-57 -0.13041536672420762 1104 58-59 -0.12270753784062904 1104 60-61 -0.3493897131063015 1104 62-63 -0.4060778856641285 1104 64-65 -0.5535194363643221 1104 66-67 -0.36674077393664106 1104 68-69 -0.5941685094924054 1104 70-71 -0.17354339869185154 1104 72-73 -0.5855011998820423 1104 74-75 -0.66529578020792 1104 76-77 -0.5674920670912798 1104 78-79 -0.5401596195717957 1104 80-81 -0.5326413829002661 1104 82-83 -0.6840680493553783 1104 84-85 -0.967997652937985 1104 86-87 -0.7535562524141071 1104 88-89 -0.5271582050172441 1104 90-91 -0.7399622743614174 1104 92-93 -0.6777045994716033 1104 94-95 -1.0844180306906992 1104 96-97 -0.3078196840229026 1104 98-99 -0.4674853570227544 1104 100-101 0.21447846467464515 1105 1 0.2855109586407032 1105 2 0.3796779315343741 1105 3 0.26771341508432656 1105 4 0.11825619936406184 1105 5 0.2934437506839558 1105 6 0.2696465089550628 1105 7 0.4747007657818685 1105 8 0.26866097777714515 1105 9 0.3887837801163556 1105 10-11 0.3395783098307703 1105 12-13 0.22228318149829107 1105 14-15 0.3445303578224781 1105 16-17 0.446579330826431 1105 18-19 0.4613763228782375 1105 20-21 0.3312020430222873 1105 22-23 0.33630331338874697 1105 24-25 0.3445275250213484 1105 26-27 0.7951119414747296 1105 28-29 0.26529256115251343 1105 30-31 0.19907895591942548 1105 32-33 0.44038051035194314 1105 34-35 0.36300209018136087 1105 36-37 0.35974240792698 1105 38-39 0.4794668695723274 1105 40-41 0.03972765801523792 1105 42-43 0.7093087314563888 1105 44-45 0.007272187035287203 1105 46-47 -0.40053365894641146 1105 48-49 0.515882046245622 1105 50-51 0.48441839156710387 1105 52-53 0.3441635534093237 1105 54-55 0.01504465965108892 1105 56-57 0.0021131898697674956 1105 58-59 0.032406688535278505 1105 60-61 0.46579287602488506 1105 62-63 0.2914601897945026 1105 64-65 0.31613701915661707 1105 66-67 -0.008896364106249166 1105 68-69 -0.09605064864090096 1105 70-71 0.34876103190697094 1105 72-73 0.5337442137558952 1105 74-75 0.2611926240842237 1105 76-77 0.4721193901603655 1105 78-79 0.4879210311724087 1105 80-81 0.3619371910007416 1105 82-83 0.39995756504406543 1105 84-85 0.6791769749609422 1105 86-87 0.49555676246993485 1105 88-89 0.774178762791685 1105 90-91 0.8287466214502821 1105 92-93 0.9928128806253156 1105 94-95 0.9900454514830663 1105 96-97 1.0860449750286705 1105 98-99 0.9818266886677964 1105 100-101 1.3910966716666842 1106 1 0.5171205121194156 1106 2 0.5464276719289742 1106 3 0.6393852738589878 1106 4 0.27985536862678373 1106 5 0.47871893240773034 1106 6 0.6525125525687727 1106 7 0.6264349298524792 1106 8 0.9256080182755895 1106 9 1.0530620771049328 1106 10-11 1.0097340730706463 1106 12-13 0.9563434099510388 1106 14-15 1.1032738676874843 1106 16-17 0.7380850421452223 1106 18-19 0.9139308400121848 1106 20-21 0.5974325726173078 1106 22-23 0.4815888793285197 1106 24-25 0.5796365592892627 1106 26-27 0.634592730675152 1106 28-29 0.5224265175388041 1106 30-31 0.5667321231053037 1106 32-33 0.4481582881297186 1106 34-35 0.4943208856123036 1106 36-37 0.5131172781242839 1106 38-39 0.727016194598292 1106 40-41 0.7334452073402673 1106 42-43 0.36385701806074877 1106 44-45 0.37569378620455396 1106 46-47 0.22215585299543505 1106 48-49 -0.06807434004721102 1106 50-51 -0.11901878392614407 1106 52-53 -0.5876225317412462 1106 54-55 -0.6796593901931445 1106 56-57 -0.3054153667242083 1106 58-59 -0.05326309339618618 1106 60-61 -0.4439267501433406 1106 62-63 -0.34598529307153925 1106 64-65 -0.35898239932728515 1106 66-67 -0.09720373689960482 1106 68-69 -0.24611295393684784 1106 70-71 -0.26900636165481373 1106 72-73 -0.04142712580796726 1106 74-75 -0.08288837280051098 1106 76-77 -0.011473548572759285 1106 78-79 -0.1870114714236486 1106 80-81 0.2885623208034396 1106 82-83 0.16408009879276975 1106 84-85 0.2009838285434995 1106 86-87 0.004684488326635261 1106 88-89 0.3386751283160905 1106 90-91 0.7415192071200636 1106 92-93 1.0591472523802459 1106 94-95 1.1913227100500414 1106 96-97 1.2864395752363578 1106 98-99 0.8294590874216912 1106 100-101 0.4318858720820522 1107 1 0.5817917139334767 1107 2 0.616813159457319 1107 3 0.6025598770335918 1107 4 0.8448440307583027 1107 5 0.7280613360358501 1107 6 0.6946894233170724 1107 7 0.848112934387629 1107 8 0.5585331883436169 1107 9 0.8471663854949583 1107 10-11 0.8359925764720018 1107 12-13 0.7803796911301788 1107 14-15 1.0065845252838557 1107 16-17 1.0908288063175675 1107 18-19 0.8599172345700126 1107 20-21 1.1796094433656066 1107 22-23 1.0334709654963206 1107 24-25 1.2467113892212325 1107 26-27 1.110465746548158 1107 28-29 1.2463720957700986 1107 30-31 1.4270495834227646 1107 32-33 1.5924213266784761 1107 34-35 1.2976768946826027 1107 36-37 1.0017794096435537 1107 38-39 0.6349980540087259 1107 40-41 0.8867331891996741 1107 42-43 1.5256257255437475 1107 44-45 1.2129273462952561 1107 46-47 1.3917703654670923 1107 48-49 1.3251229388643537 1107 50-51 0.7436116015613834 1107 52-53 1.2620826836782584 1107 54-55 1.1012929907592337 1107 56-57 1.0043352001692156 1107 58-59 1.0086416685085773 1107 60-61 1.0740324335301317 1107 62-63 0.9665317137311824 1107 64-65 0.8840561494255503 1107 66-67 0.9510502313543618 1107 68-69 1.0317101753148528 1107 70-71 1.1174335476422357 1107 72-73 1.2909538265729843 1107 74-75 1.3810572054307784 1107 76-77 1.1236738437174907 1107 78-79 1.1368434038597997 1107 80-81 1.3566348831617177 1107 82-83 1.3813136588834745 1107 84-85 1.4445212435094845 1107 86-87 1.1178817672381989 1107 88-89 1.3318270557537275 1107 90-91 1.4387981186846908 1107 92-93 1.7932515607702726 1107 94-95 1.9143159073289517 1107 96-97 1.6711107770504157 1107 98-99 1.5500033051087652 1107 100-101 1.8673507246897607 1108 1 0.23192337950293052 1108 2 0.20542408769958342 1108 3 -0.017370998542446614 1108 4 0.013869705544344413 1108 5 0.10735692523927298 1108 6 0.21353405794511815 1108 7 0.13957113056932258 1108 8 0.2813607064476358 1108 9 0.21309791939884093 1108 10-11 0.3636588042534399 1108 12-13 0.3991749511696767 1108 14-15 0.2704423264688458 1108 16-17 0.1309703468047232 1108 18-19 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5059.0 3 731.0 4 570.0 5 491.0 6 492.0 7 498.0 8 607.0 9 766.0 10 1029.0 11 1525.0 12 2351.0 13 3523.0 14 4220.0 15 6049.0 16 8117.0 17 10572.0 18 9208.0 19 4695.0 20 4202.0 21 3778.0 22 3789.0 23 3758.0 24 3905.0 25 4072.0 26 4240.0 27 4499.0 28 5106.0 29 5632.0 30 6701.0 31 7689.0 32 9060.0 33 11506.0 34 17605.0 35 32009.0 36 18150.0 37 16272.0 38 10125.0 39 599.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.433014694162395 21.284780707611585 15.50275057833225 11.779454019893768 2 17.3984660524013 26.34606475523756 38.778504143717505 17.47696504864364 3 19.231297244888328 36.399478322050335 31.55851287463427 12.810711558427073 4 10.817420104939357 23.743065893850794 34.668600136429745 30.770913864780102 5 8.9156667868239 43.51322587843521 29.073759376555607 18.49734795818528 6 20.98356434793803 41.36806419774278 17.203364373685794 20.445007080633395 7 20.06366420990313 46.61129653622081 18.038335807257035 15.286703446619018 8 27.551887156695354 32.17947507957889 17.37050733155443 22.898130432171325 9 31.043758043758046 17.937794937794937 19.313599313599315 31.704847704847705 10-11 23.777797321675635 33.33447736637873 20.629060870423242 22.258664441522395 12-13 29.355895266755756 34.09707580011712 23.809074393034944 12.73795454009219 14-15 24.736563279505663 31.6375007774949 30.679627059825282 12.94630888317415 16-17 14.680233181625152 36.101971920402534 29.88143598014782 19.336358917824494 18-19 13.819826815127186 29.000422559077833 42.689835201959646 14.489915423835335 20-21 17.85919601870522 26.703633789523362 43.583808829207605 11.853361362563817 22-23 16.585658823277065 28.973638489092895 43.03811587053045 11.40258681709959 24-25 14.429756250348614 29.983223708204076 43.991856418969654 11.595163622477656 26-27 13.53255879804021 30.528431566031422 44.36946019923976 11.569549436688604 28-29 13.333719413843989 31.304480249836985 43.76501424208106 11.596786094237963 30-31 13.288147226631203 31.796147741409637 43.36858135644117 11.547123675517994 32-33 12.11700030672149 32.426038295143286 43.475090139654796 11.98187125848042 34-35 12.278368604167293 32.50929854440779 43.40736418977181 11.804968661653103 36-37 12.786388067896654 32.338848399315374 42.4053809427803 12.469382590007678 38-39 12.440811144660993 32.93345113917101 42.325178424375515 12.300559291792478 40-41 12.22666126261749 33.11567024148809 42.003615566234984 12.654052929659438 42-43 12.075879550006647 33.13039599586548 41.383776736047075 13.409947718080794 44-45 12.643298579987732 32.95299077485236 40.807875900105934 13.595834745053976 46-47 12.755060459569942 33.34241263495631 39.69913911062879 14.203387794844957 48-49 12.7216498382932 33.50805102557284 38.16344825811322 15.606850878020742 50-51 13.118093722726035 33.85949525339189 36.757424415600006 16.264986608282065 52-53 13.515292785596497 34.10924858094358 35.6120438057946 16.763414827665326 54-55 13.60515334371207 33.327114667901824 35.36233375791807 17.70539823046803 56-57 14.351698967710394 33.4374772162919 34.3050808205137 17.905742995484 58-59 14.58046089154875 32.82313763919058 34.605034340644615 17.99136712861606 60-61 15.005039133697867 32.927415031628605 34.83585290018227 17.23169293449126 62-63 15.751168675215506 32.60174979628597 34.76111849723378 16.88596303126474 64-65 16.187249372761777 32.91124311110158 34.65614479017005 16.24536272596659 66-67 16.239176955438435 32.4659173267348 34.46070253834971 16.834203179477065 68-69 16.237635651351635 32.08501568527385 34.77692174088314 16.900426922491373 70-71 16.149085637823372 32.08973787140603 35.00952103204556 16.75165545872504 72-73 15.890269373121013 31.34201937633754 35.3118526746465 17.455858575894943 74-75 15.097200005146261 31.863718430905013 35.264110404453234 17.774971159495497 76-77 14.544245984947574 31.388168192636744 35.916332525677035 18.15125329673864 78-79 14.305227945275977 31.038512673156927 36.132649997855644 18.523609383711456 80-81 14.60266469948683 30.632249597378618 35.60974354465505 19.155342158479495 82-83 14.799200576408428 30.56251286625952 34.68035922596583 19.957927331366225 84-85 15.660214306698316 29.850474223797608 34.409369967470155 20.07994150203392 86-87 15.441335703304738 29.77973417154276 34.425192595912016 20.353737529240487 88-89 16.101173306683194 29.19691435997847 34.22941566281855 20.472496670519785 90-91 16.570872187288217 29.03859516681107 34.10276317031114 20.287769475589563 92-93 17.087747137281813 28.634472702320195 34.34339752112193 19.934382639276066 94-95 17.309164684663045 28.218084022655965 34.44075328290573 20.03199800977526 96-97 17.813416143207323 27.878847667433714 34.14298857575452 20.16474761360444 98-99 18.13828612356671 27.82449074997373 33.73477182124836 20.302451305211193 100-101 19.56129220635626 26.73296448729757 33.99545275958237 19.710290546763794 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7720.0 1 4754.5 2 2032.0 3 2185.5 4 1637.5 5 1498.5 6 2667.5 7 5219.5 8 7597.0 9 7724.5 10 5279.5 11 2953.5 12 2476.0 13 2364.5 14 2208.5 15 1912.0 16 1652.5 17 1544.5 18 1491.5 19 1454.5 20 1457.0 21 1523.0 22 1596.5 23 1660.0 24 1800.0 25 2108.0 26 2552.0 27 3029.5 28 3268.0 29 3457.5 30 3916.0 31 4443.5 32 4795.5 33 5228.0 34 5849.0 35 6250.5 36 6194.0 37 6130.0 38 5833.5 39 5192.0 40 4872.0 41 4715.5 42 4614.0 43 4612.0 44 4658.0 45 4518.5 46 4252.0 47 4410.5 48 5010.5 49 5152.5 50 4807.5 51 4310.0 52 3760.0 53 3317.0 54 2878.0 55 2744.5 56 2913.5 57 2988.5 58 2770.0 59 2683.5 60 2949.0 61 3257.0 62 2756.5 63 1444.5 64 615.0 65 436.0 66 392.0 67 345.0 68 306.5 69 280.0 70 248.5 71 242.5 72 233.0 73 218.0 74 196.0 75 144.5 76 98.0 77 74.5 78 58.0 79 37.0 80 23.5 81 17.0 82 9.5 83 6.0 84 7.5 85 5.0 86 3.5 87 4.5 88 2.5 89 1.5 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4575471698113207 2 0.032590051457975985 3 0.045454545454545456 4 0.04845626072041166 5 0.07375643224699828 6 0.07289879931389365 7 0.04373927958833619 8 0.04202401372212693 9 0.04288164665523156 10-11 0.04566895368782161 12-13 0.04009433962264151 14-15 0.034090909090909095 16-17 0.0330188679245283 18-19 0.041380789022298456 20-21 0.04716981132075472 22-23 0.040737564322469985 24-25 0.05703259005145798 26-27 0.04888507718696398 28-29 0.03773584905660377 30-31 0.038593481989708404 32-33 0.03837907375643224 34-35 0.04245283018867924 36-37 0.03473413379073756 38-39 0.020583190394511147 40-41 0.017795883361921096 42-43 0.017581475128644938 44-45 0.014150943396226415 46-47 0.02958833619210978 48-49 0.02658662092624357 50-51 0.014365351629502573 52-53 0.01479416809605489 54-55 0.013293310463121785 56-57 0.012435677530017153 58-59 0.009219554030874785 60-61 0.01072041166380789 62-63 0.012864493996569469 64-65 0.015008576329331048 66-67 0.007289879931389366 68-69 0.008361921097770154 70-71 0.0137221269296741 72-73 0.012435677530017153 74-75 0.009005145797598627 76-77 0.006432246998284734 78-79 0.012864493996569469 80-81 0.01736706689536878 82-83 0.0137221269296741 84-85 0.01265008576329331 86-87 0.0019296740994854203 88-89 0.023799313893653515 90-91 0.02658662092624357 92-93 0.012864493996569469 94-95 0.025943396226415096 96-97 0.023799313893653515 98-99 0.01650943396226415 100-101 0.13357632933104632 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 233200.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.976843910806174 #Duplication Level Percentage of deduplicated Percentage of total 1 80.8713862498723 33.94725557461407 2 9.747675962815405 8.183533447684392 3 3.556032281131883 4.478130360205832 4 1.6825007661661049 2.825042881646655 5 0.9071406680968434 1.9039451114922814 6 0.6292777607518644 1.5849056603773584 7 0.4076003677597303 1.1976843910806174 8 0.30953110634385533 1.039451114922813 9 0.2564102564102564 0.9686963979416809 >10 1.3647972213709265 10.879931389365352 >50 0.15629788538155073 4.61106346483705 >100 0.0970477066094596 7.073327615780446 >500 0.004086219225661457 1.0180102915951972 >1k 0.006129328838492185 4.733704974271012 >5k 0.0030646644192460926 10.611921097770155 >10k+ 0.0010215548064153642 4.943396226415095 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11528 4.943396226415095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9651 4.138507718696398 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8551 3.666809605488851 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6545 2.806603773584906 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2873 1.2319897084048026 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2274 0.9751286449399658 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1858 0.7967409948542025 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1694 0.7264150943396226 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1229 0.5270154373927959 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1111 0.47641509433962265 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.2993138936535163 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 615 0.2637221269296741 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 540 0.23156089193825044 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 521 0.22341337907375644 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 443 0.1899656946826758 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 431 0.18481989708404803 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 389 0.16680960548885077 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 382 0.16380789022298456 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 372 0.1595197255574614 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 366 0.1569468267581475 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 364 0.15608919382504288 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 349 0.14965694682675815 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 342 0.14665523156089194 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 338 0.14493996569468268 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 311 0.13336192109777015 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 290 0.12435677530017153 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 282 0.12092624356775301 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 274 0.11749571183533448 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 261 0.11192109777015437 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 254 0.10891938250428818 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 253 0.10849056603773584 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 247 0.10591766723842194 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.288164665523156E-4 0.0 20-21 0.0 0.0 0.0 0.00192967409948542 0.0 22-23 0.0 0.0 0.0 0.010934819897084048 0.0 24-25 0.0 0.0 0.0 0.03473413379073756 0.0 26-27 0.0 0.0 0.0 0.07590051457975987 0.0 28-29 0.0 0.0 0.0 0.16895368782161235 0.0 30-31 0.0 0.0 0.0 0.3814322469982847 0.0 32-33 0.0 0.0 0.0 0.6114922813036021 0.0 34-35 0.0 0.0 0.0 0.8655660377358491 0.0 36-37 0.0 0.0 0.0 1.169596912521441 0.0 38-39 0.0 0.0 0.0 1.5617495711835334 0.0 40-41 0.0 0.0 0.0 1.983704974271012 0.0 42-43 0.0 0.0 0.0 2.3228987993138936 0.0 44-45 0.0 0.0 0.0 2.6393653516295026 0.0 46-47 0.0 0.0 0.0 2.978130360205832 0.0 48-49 0.0 0.0 0.0 3.3173241852487134 0.0 50-51 0.0 0.0 0.0 3.691680960548885 0.0 52-53 0.0 0.0 0.0 4.003859348198971 0.0 54-55 0.0 0.0 0.0 4.288807890222985 0.0 56-57 0.0 0.0 0.0 4.582761578044597 0.0 58-59 0.0 0.0 0.0 4.873713550600343 0.0 60-61 0.0 0.0 0.0 5.206046312178388 0.0 62-63 0.0 0.0 0.0 5.533876500857633 0.0 64-65 0.0 0.0 0.0 5.819897084048028 0.0 66-67 0.0 0.0 0.0 6.126072041166381 0.0 68-69 0.0 0.0 0.0 6.4352487135506 0.0 70-71 0.0 0.0 0.0 6.79631217838765 0.0 72-73 0.0 0.0 0.0 7.120283018867925 0.0 74-75 0.0 0.0 0.0 7.447255574614065 0.0 76-77 0.0 0.0 0.0 7.737135506003431 0.0 78-79 0.0 0.0 0.0 8.077186963979416 0.0 80-81 0.0 0.0 0.0 8.417667238421956 0.0 82-83 0.0 0.0 0.0 8.769725557461406 0.0 84-85 0.0 0.0 0.0 9.107847341337909 0.0 86-87 0.0 0.0 0.0 9.434391080617495 0.0 88-89 0.0 0.0 0.0 9.783662092624358 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 65 0.0 73.37596 1 CCTGTAG 25 0.00501201 57.01223 3 GATAGCA 25 0.0050162687 57.0 9 GGTATCA 3705 0.0 49.68969 1 ATGAGAG 45 9.599122E-4 42.23128 5 GTCACTA 120 8.54925E-11 39.591824 2 TGGGCAA 60 8.259961E-5 39.591824 6 GTATCAA 6205 0.0 38.50908 1 CGAGTAC 25 0.0016148308 38.0 10-11 TGTGAAG 55 0.0025727174 34.552864 3 GTATATG 85 1.5817846E-5 33.666622 1 TACATGA 60 0.0039404077 31.67346 2 CAGTGGA 60 0.0039445497 31.666668 7 GGCATTC 60 0.0039445497 31.666668 7 CACGCTC 30 0.003956865 31.653086 90-91 ATGGGCA 170 1.07320375E-10 30.741888 5 TATCAAC 7745 0.0 30.732868 2 ATGGGTG 80 4.477147E-4 29.69387 5 ACTACCT 160 1.884473E-9 29.69387 5 ATCAACG 8060 0.0 29.531769 3 >>END_MODULE