##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938274_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 233200 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3008704974271 33.0 31.0 34.0 31.0 34.0 2 32.457903087478556 34.0 31.0 34.0 31.0 34.0 3 32.29945111492281 34.0 31.0 34.0 30.0 34.0 4 35.96485420240137 37.0 35.0 37.0 35.0 37.0 5 36.00235420240137 37.0 35.0 37.0 35.0 37.0 6 36.13690394511149 37.0 36.0 37.0 35.0 37.0 7 36.080617495711834 37.0 35.0 37.0 35.0 37.0 8 36.117684391080616 37.0 36.0 37.0 35.0 37.0 9 37.678331903945114 39.0 37.0 39.0 35.0 39.0 10-11 37.69533876500858 39.0 37.5 39.0 35.0 39.0 12-13 37.61413164665523 39.0 37.0 39.0 35.0 39.0 14-15 38.44603344768439 40.0 38.0 41.0 34.0 41.0 16-17 38.44559176672384 40.0 38.0 41.0 34.5 41.0 18-19 38.64680960548885 40.0 38.0 41.0 35.0 41.0 20-21 38.790891938250425 40.0 38.0 41.0 35.0 41.0 22-23 38.622630789022296 40.0 38.0 41.0 35.0 41.0 24-25 38.44338121783876 40.0 37.0 41.0 35.0 41.0 26-27 38.08150514579759 40.0 36.5 41.0 34.0 41.0 28-29 37.764313893653515 39.5 35.0 41.0 33.5 41.0 30-31 37.009841337907375 38.5 35.0 40.5 33.0 41.0 32-33 36.151616638078906 36.5 35.0 40.0 33.0 41.0 34-35 35.593696397941684 35.0 35.0 40.0 32.0 41.0 36-37 35.08904373927959 35.0 35.0 40.0 31.0 41.0 38-39 34.52200686106347 35.0 35.0 40.0 28.5 41.0 40-41 34.04530231560892 35.0 35.0 40.0 25.0 41.0 42-43 33.313599914236704 35.0 34.5 40.0 19.5 41.0 44-45 32.33590480274442 35.0 33.0 40.0 4.0 41.0 46-47 31.02206475128645 35.0 32.5 40.0 2.0 41.0 48-49 29.908550600343055 35.0 30.5 39.0 2.0 41.0 50-51 29.234886363636363 35.0 28.0 39.0 2.0 41.0 52-53 28.669554030874785 35.0 26.5 38.5 2.0 41.0 54-55 28.249251715265867 35.0 23.5 38.0 2.0 41.0 56-57 27.794281732418526 35.0 22.5 37.5 2.0 40.0 58-59 27.288795025728987 35.0 19.0 37.0 2.0 40.0 60-61 26.76597126929674 35.0 9.5 36.5 2.0 40.0 62-63 26.206766723842197 35.0 2.0 36.0 2.0 40.0 64-65 25.675270154373926 34.0 2.0 35.0 2.0 39.5 66-67 25.02682461406518 33.5 2.0 35.0 2.0 39.0 68-69 24.391125643224697 33.0 2.0 35.0 2.0 39.0 70-71 23.834138078902228 33.0 2.0 35.0 2.0 38.0 72-73 23.462107632933105 33.0 2.0 35.0 2.0 37.5 74-75 23.004974271012006 33.0 2.0 35.0 2.0 37.0 76-77 21.93069897084048 30.0 2.0 34.5 2.0 35.5 78-79 22.48148370497427 32.0 2.0 35.0 2.0 36.0 80-81 22.33860420240137 33.0 2.0 35.0 2.0 35.5 82-83 21.98230703259005 32.0 2.0 35.0 2.0 35.0 84-85 21.59429459691252 31.5 2.0 35.0 2.0 35.0 86-87 21.159785591766724 31.0 2.0 35.0 2.0 35.0 88-89 20.696001286449402 30.5 2.0 35.0 2.0 35.0 90-91 20.285328044596913 29.5 2.0 35.0 2.0 35.0 92-93 19.82427530017153 29.0 2.0 35.0 2.0 35.0 94-95 19.29988636363636 28.0 2.0 34.0 2.0 35.0 96-97 18.761653087478557 26.5 2.0 34.0 2.0 35.0 98-99 18.187746569468267 24.5 2.0 34.0 2.0 35.0 100-101 16.21153730703259 11.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05009357281230109 1101 2 -0.2536873700932034 1101 3 -0.0488443940015415 1101 4 -0.21696581601204912 1101 5 -0.009178065247681388 1101 6 -0.04614844584711619 1101 7 0.04268392097407059 1101 8 0.053962365586116334 1101 9 0.006283781111299902 1101 10-11 -0.20594380159629821 1101 12-13 -0.26178625208059003 1101 14-15 0.0010725042163954868 1101 16-17 -0.1192217422472055 1101 18-19 -0.2375519550426688 1101 20-21 -0.12074783488187535 1101 22-23 0.08758615859056817 1101 24-25 -0.12067801122832122 1101 26-27 0.17694533087031772 1101 28-29 0.11614089927699922 1101 30-31 -0.09588771404828833 1101 32-33 -0.13017753030555212 1101 34-35 -0.1256243314178178 1101 36-37 0.05749841098470654 1101 38-39 -0.3174538772792417 1101 40-41 0.15006168379652252 1101 42-43 0.5452479591980364 1101 44-45 0.27390552624952846 1101 46-47 0.628452492464934 1101 48-49 -0.03990948784904802 1101 50-51 -0.15403776662433089 1101 52-53 0.3117690624656504 1101 54-55 0.20634422422164178 1101 56-57 0.7144292555354781 1101 58-59 0.7905930274429984 1101 60-61 0.10289704969914837 1101 62-63 0.31778315705966165 1101 64-65 0.4242084060900382 1101 66-67 -5.847826747782392E-4 1101 68-69 0.03038383080949103 1101 70-71 -0.3383208510390894 1101 72-73 -0.09615626936055932 1101 74-75 0.26232465235115043 1101 76-77 0.38726056506210327 1101 78-79 0.5926719316355893 1101 80-81 0.25692761142288134 1101 82-83 0.22414327901871545 1101 84-85 0.30025644494751447 1101 86-87 0.3807535132947244 1101 88-89 0.24505764793606843 1101 90-91 0.3594601749725008 1101 92-93 0.14330957528888888 1101 94-95 0.5032828299180814 1101 96-97 0.5555070170825651 1101 98-99 0.6857154327037485 1101 100-101 0.3778146794767032 1102 1 -0.08878096486756704 1102 2 -0.11284108339199861 1102 3 -0.08753178605680745 1102 4 0.08965479423550704 1102 5 0.14588814085479385 1102 6 -0.027351669796452427 1102 7 0.0744052796384338 1102 8 0.026443655165849123 1102 9 -0.09898392182479654 1102 10-11 -0.08049763003614174 1102 12-13 -0.05538153129763401 1102 14-15 0.12651867577655196 1102 16-17 -0.13134033752642438 1102 18-19 0.08705944392105636 1102 20-21 -0.026346568330346543 1102 22-23 0.044278731992982046 1102 24-25 -0.07208848906366683 1102 26-27 0.060321842096563216 1102 28-29 -0.013335208955588485 1102 30-31 0.20575304588263066 1102 32-33 0.35678274603296245 1102 34-35 0.37100779293451325 1102 36-37 0.44483289572852414 1102 38-39 0.8409082413160363 1102 40-41 0.7754934627257128 1102 42-43 0.8519117473385052 1102 44-45 1.3292595849714601 1102 46-47 1.2059712028852054 1102 48-49 0.8809022565378264 1102 50-51 0.6663134135714088 1102 52-53 0.6640143128974287 1102 54-55 0.8740903382112784 1102 56-57 0.9056497506419845 1102 58-59 0.6486529007878445 1102 60-61 0.05901391786264654 1102 62-63 -0.05210745894494195 1102 64-65 -0.08488773668485905 1102 66-67 0.06868695019799276 1102 68-69 0.181707952859 1102 70-71 0.35957782483886547 1102 72-73 0.3285270927580335 1102 74-75 0.015548601689086894 1102 76-77 -0.354175819508999 1102 78-79 -0.26685599006849614 1102 80-81 -0.26525143290642106 1102 82-83 -0.3260870030768537 1102 84-85 -0.34765662356140936 1102 86-87 -0.33535184076399815 1102 88-89 0.104355287544589 1102 90-91 0.08277623714866778 1102 92-93 0.46131475663603894 1102 94-95 0.33214005501882937 1102 96-97 0.13659509998412034 1102 98-99 0.3532024793358737 1102 100-101 0.6561825551243707 1103 1 0.2130016652829383 1103 2 0.16655072514488722 1103 3 0.3630603679032234 1103 4 -0.07371184775807649 1103 5 -0.20520981127942406 1103 6 -0.032656382355050084 1103 7 -5.700472799006207E-4 1103 8 0.05475601637976979 1103 9 0.25985520968272624 1103 10-11 0.17361969046718428 1103 12-13 0.35587247807813327 1103 14-15 0.3240883772322718 1103 16-17 0.3716512736258153 1103 18-19 0.30947185448113856 1103 20-21 0.5582204190863749 1103 22-23 0.3203242538286659 1103 24-25 0.1904330998827959 1103 26-27 0.2584929499179296 1103 28-29 0.33082343895953414 1103 30-31 0.4862551430945672 1103 32-33 0.03887008874207254 1103 34-35 0.22616138286790033 1103 36-37 0.39995872844502856 1103 38-39 0.4660778687525067 1103 40-41 0.5667283504631939 1103 42-43 0.7200495464996237 1103 44-45 0.9977150500590497 1103 46-47 1.2312302702427154 1103 48-49 1.2654476550080922 1103 50-51 1.6344542968677338 1103 52-53 1.9718881100846986 1103 54-55 1.9712251766025943 1103 56-57 2.3360562396624616 1103 58-59 2.520553344903316 1103 60-61 2.659047843349942 1103 62-63 2.60151331578982 1103 64-65 2.8704385648202013 1103 66-67 3.0617168046268084 1103 68-69 3.409748910174571 1103 70-71 3.0436235934053606 1103 72-73 3.0260659528616607 1103 74-75 3.1416897317162302 1103 76-77 2.507300247601787 1103 78-79 3.45080685227051 1103 80-81 3.5579196749149453 1103 82-83 3.303508358383791 1103 84-85 3.175058032249101 1103 86-87 2.4819439894852025 1103 88-89 2.5877163780948003 1103 90-91 2.5475554130677445 1103 92-93 2.0421190990984144 1103 94-95 1.7596320362672877 1103 96-97 2.3912213027968505 1103 98-99 2.6392868612751776 1103 100-101 2.179005155667177 1104 1 0.20175827903955224 1104 2 0.2287200373142042 1104 3 0.22152597636883087 1104 4 0.06914529509906231 1104 5 -0.10269658376619617 1104 6 -0.012815112513784754 1104 7 0.328795032085182 1104 8 0.05211051373426301 1104 9 0.12295044777796704 1104 10-11 0.17924138358888086 1104 12-13 0.2761767108823676 1104 14-15 0.29366509680897934 1104 16-17 0.31874122071576494 1104 18-19 0.21291100792029027 1104 20-21 0.190826239192198 1104 22-23 0.1625861585905639 1104 24-25 0.16959976654946018 1104 26-27 0.3153712567962401 1104 28-29 -0.030618359982263144 1104 30-31 0.024945619285041687 1104 32-33 0.1559335808055593 1104 34-35 0.09520900191551362 1104 36-37 0.23527618876248368 1104 38-39 -0.03226869209405692 1104 40-41 0.19172835046318681 1104 42-43 0.16052573697581352 1104 44-45 -0.009890770046773412 1104 46-47 -0.41691787790543344 1104 48-49 -0.10194652488608469 1104 50-51 0.13114741856085388 1104 52-53 -0.42989760420101675 1104 54-55 -0.3214335535561368 1104 56-57 -0.6874225963163738 1104 58-59 -0.6325551207051525 1104 60-61 -0.3429362836341845 1104 62-63 -0.7817538799773764 1104 64-65 -0.6470878902062545 1104 66-67 -0.3357699678599637 1104 68-69 -0.2668383914127297 1104 70-71 -0.22952455474279176 1104 72-73 -0.21235997306426313 1104 74-75 -0.305730903204406 1104 76-77 -0.61736906456753 1104 78-79 -0.6202910313273726 1104 80-81 -0.5657575737623048 1104 82-83 -0.23419005431462026 1104 84-85 -0.6483546661635984 1104 86-87 -0.059061301520088705 1104 88-89 -0.1378127224343011 1104 90-91 -0.47063241762009156 1104 92-93 -0.5279867210074087 1104 94-95 -0.3981060589708072 1104 96-97 -0.46162261254706394 1104 98-99 0.02321543270374704 1104 100-101 0.2236480128100382 1105 1 0.05239863902570363 1105 2 -0.0030643171337061403 1105 3 -0.10523068683642833 1105 4 0.1396281611426815 1105 5 0.06470563153321507 1105 6 0.0585763724291084 1105 7 0.019734803632431408 1105 8 0.01637150369619178 1105 9 -0.014121203312377872 1105 10-11 0.09893677991978933 1105 12-13 0.1680943294354975 1105 14-15 0.013897010966132939 1105 16-17 0.1043504280539338 1105 18-19 0.05310225056480533 1105 20-21 0.17938196781802418 1105 22-23 0.1493462832011616 1105 24-25 0.3298411995712627 1105 26-27 0.36160265174258654 1105 28-29 0.39952615369028877 1105 30-31 0.5189097937398728 1105 32-33 0.8419408497567531 1105 34-35 0.7729737994233048 1105 36-37 0.9632097297801394 1105 38-39 0.9031328309242852 1105 40-41 0.561666045167243 1105 42-43 0.6798014379103918 1105 44-45 0.9684538128538875 1105 46-47 1.4637069057567338 1105 48-49 1.7127384872288296 1105 50-51 1.3249861170724522 1105 52-53 1.0479061341167402 1105 54-55 0.9528395513244483 1105 56-57 1.0509816958262377 1105 58-59 1.3215379910982392 1105 60-61 1.2677205180272892 1105 62-63 1.322196448856129 1105 64-65 1.1656933489768484 1105 66-67 1.891601094791472 1105 68-69 1.812730663623615 1105 70-71 1.8031640918477265 1105 72-73 1.8870472612728761 1105 74-75 1.6365717966917508 1105 76-77 1.8609106169831797 1105 78-79 2.202225410347946 1105 80-81 2.1738798440293188 1105 82-83 2.2228452520197486 1105 84-85 2.394934534251771 1105 86-87 2.366345413606254 1105 88-89 1.9832715627854967 1105 90-91 2.232357371234187 1105 92-93 2.079057238840292 1105 94-95 2.630515436356294 1105 96-97 2.4302733722227536 1105 98-99 2.666322909339261 1105 100-101 2.5065529972337117 1106 1 0.025461982743252065 1106 2 0.15631262990679318 1106 3 0.17782227266512507 1106 4 0.19636751732128488 1106 5 0.034155268085655166 1106 6 0.1460737763751041 1106 7 -0.0884271901370397 1106 8 0.14729569891945005 1106 9 0.2273948922224065 1106 10-11 0.21850064284814863 1106 12-13 0.19876930347496824 1106 14-15 0.07885028199417121 1106 16-17 0.05522270219724135 1106 18-19 -0.020885288376007338 1106 20-21 -0.10130339043742964 1106 22-23 0.027030603035008482 1106 24-25 0.0037664332161213565 1106 26-27 -0.33138800246302225 1106 28-29 0.0072520103881075215 1106 30-31 0.1874456192850431 1106 32-33 0.2931558030277799 1106 34-35 0.2465978908044093 1106 36-37 0.4352761887624865 1106 38-39 0.48199056716520516 1106 40-41 0.2583950171298568 1106 42-43 -0.5841964852464088 1106 44-45 -0.6205389181949172 1106 46-47 -0.10376972975728549 1106 48-49 0.5478682899287293 1106 50-51 0.831517788931226 1106 52-53 0.7662135069100948 1106 54-55 0.7435664464438645 1106 56-57 0.8155403666465872 1106 58-59 0.6272596941096644 1106 60-61 0.5845637163658175 1106 62-63 0.6400053792818845 1106 64-65 1.0330972949789263 1106 66-67 1.3310818839918888 1106 68-69 0.3848282752539376 1106 70-71 0.7539013711831366 1106 72-73 0.9166215084172187 1106 74-75 0.9612135412400384 1106 76-77 0.7678161206176561 1106 78-79 1.1126719316355924 1106 80-81 1.2497053892006598 1106 82-83 1.2963655012409347 1106 84-85 0.9463675560586253 1106 86-87 0.9279757355169451 1106 88-89 0.9739465368249576 1106 90-91 0.8083490638613924 1106 92-93 0.3983095752888879 1106 94-95 0.23494949658474695 1106 96-97 0.14106257263811983 1106 98-99 -0.007062345074029253 1106 100-101 0.2783702350322592 1107 1 0.07915812786797005 1107 2 0.36638065711768064 1107 3 0.32530526586240427 1107 4 -0.0051744327920886235 1107 5 -0.10861117182363955 1107 6 -0.16394889936185564 1107 7 0.0696680479581957 1107 8 -0.10340724892635222 1107 9 0.10594364505687537 1107 10-11 0.1555074455692349 1107 12-13 -0.007988066139553496 1107 14-15 -0.3007415547405259 1107 16-17 -0.20981131140820253 1107 18-19 0.07367253475324986 1107 20-21 0.1714857252088322 1107 22-23 -0.05033901147746178 1107 24-25 -0.17011111780428223 1107 26-27 -0.3273063698099534 1107 28-29 -0.16730581274114087 1107 30-31 -0.35473125146324946 1107 32-33 -0.159089094931403 1107 34-35 -0.2041957599892399 1107 36-37 -0.4025922919631313 1107 38-39 -0.07737451219987435 1107 40-41 -0.4293600849109609 1107 42-43 -0.6427905895547994 1107 44-45 -0.5427611404171415 1107 46-47 -0.272341158328711 1107 48-49 -0.4212020048558536 1107 50-51 -0.2677565874860086 1107 52-53 -0.5640812776704038 1107 54-55 -0.2655945512885616 1107 56-57 0.1573997770774298 1107 58-59 0.30499212041351953 1107 60-61 -0.07198957161604724 1107 62-63 -0.05750028965235643 1107 64-65 -0.08917027871721572 1107 66-67 0.10899571619143344 1107 68-69 0.3386604748004203 1107 70-71 0.2391167906842675 1107 72-73 0.06024962633104636 1107 74-75 0.4760434732128296 1107 76-77 0.2587458258330777 1107 78-79 -0.2846523314029561 1107 80-81 -0.16512454277212996 1107 82-83 -0.21247803617402994 1107 84-85 0.013578440412366888 1107 86-87 -0.15411043228350252 1107 88-89 0.1908626365982009 1107 90-91 -0.1099275801295363 1107 92-93 0.08973814671745828 1107 94-95 0.38225108388633444 1107 96-97 0.17897640483766963 1107 98-99 0.3209195143363992 1107 100-101 0.659957536619558 1108 1 -0.05869572334993478 1108 2 -0.13567661740503212 1108 3 -0.025188480023047077 1108 4 -0.03703750059985822 1108 5 -0.034894911125817885 1108 6 0.07410245021023343 1108 7 0.05952979911027256 1108 8 0.14231362006640325 1108 9 0.06381066283172743 1108 10-11 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-0.42970540621639586 2316 50-51 0.11319579346637099 2316 52-53 0.5539459332139494 2316 54-55 0.5507319793236825 2316 56-57 0.8220256274175632 2316 58-59 1.060094161229845 2316 60-61 0.5956975032138878 2316 62-63 0.23841807769458256 2316 64-65 0.6625304015548927 2316 66-67 0.7215807502050389 2316 68-69 0.8689325836439608 2316 70-71 1.0976201920448112 2316 72-73 0.9010659528616607 2316 74-75 0.743730548042759 2316 76-77 0.42099072379226143 2316 78-79 0.2650075325426222 2316 80-81 0.20120198784011478 2316 82-83 -0.19479096134410057 2316 84-85 0.1941906853103248 2316 86-87 0.2897671187369042 2316 88-89 0.3177333848975188 2316 90-91 0.05605881442828675 2316 92-93 -0.20788090090158562 2316 94-95 0.20435992742374864 2316 96-97 -0.290751486318797 2316 98-99 -0.5035702815819683 2316 100-101 -0.8703145722239771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 9.0 5 34.0 6 70.0 7 124.0 8 271.0 9 473.0 10 812.0 11 1521.0 12 2160.0 13 3221.0 14 4227.0 15 5492.0 16 7581.0 17 10624.0 18 9296.0 19 5348.0 20 4064.0 21 3962.0 22 4179.0 23 4375.0 24 4545.0 25 4819.0 26 4377.0 27 4009.0 28 4312.0 29 4834.0 30 5589.0 31 6289.0 32 7653.0 33 9860.0 34 14943.0 35 33180.0 36 22624.0 37 21863.0 38 15328.0 39 1127.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.531303602058316 21.86320754716981 15.717838765008576 12.887650085763294 2 16.788825176804608 26.325337633541622 38.87514099336527 18.010696196288496 3 19.014353287275878 35.93081947106828 32.75053926676873 12.304287974887108 4 10.22598627787307 24.568610634648373 33.48842195540308 31.71698113207547 5 8.400550602704127 44.239469294465245 29.64120772387532 17.718772378955315 6 20.638078902229847 41.21097770154374 16.799313893653515 21.351629502572898 7 19.13164665523156 46.51715265866209 17.499571183533448 16.851629502572898 8 27.492710120068608 32.59862778730703 17.741852487135507 22.16680960548885 9 29.624785591766724 17.590480274442537 18.937821612349914 33.84691252144083 10-11 23.54566895368782 32.97877358490566 20.955617495711834 22.519939965694682 12-13 28.86299313893653 33.4639794168096 23.847555746140653 13.825471698113207 14-15 24.272084048027445 31.707547169811324 29.75814751286449 14.26222126929674 16-17 14.824645850441359 36.21277061057164 29.382567286808076 19.58001625217893 18-19 13.798456260720412 28.925385934819897 41.563893653516296 15.712264150943398 20-21 17.531089193825043 26.67860205831904 42.57161234991423 13.218696397941681 22-23 16.260934819897084 29.712264150943398 42.09884219554031 11.927958833619211 24-25 14.466095189903816 30.94508076862052 42.924222867361074 11.66460117411459 26-27 13.621166272419035 31.576125535880106 43.33578531768924 11.46692287401161 28-29 12.933346769611429 31.75242657038287 42.92676442273676 12.387462237268949 30-31 13.876500857632934 32.43739279588336 42.50493138936535 11.181174957118353 32-33 12.795095781497254 32.75675745217358 42.884680535364325 11.563466230964847 34-35 12.295502064011151 33.089154559588266 43.07296413445558 11.542379241944996 36-37 12.59660002230056 32.5718978634348 42.129832145399654 12.701669968864987 38-39 12.069845545437778 32.75243091150067 42.12328914014146 13.054434402920096 40-41 11.782922461584654 32.95816935994081 42.22782204016857 13.031086138305973 42-43 12.060964259520572 32.75617298512136 41.80881223917342 13.374050516184651 44-45 11.951452251015878 33.0495663082053 40.866311421801456 14.132670018977366 46-47 13.035283926687653 32.83467842225786 39.201694726279406 14.928342924775079 48-49 12.625295617253112 32.84026269465713 38.161266045879366 16.37317564221039 50-51 12.568396226415093 33.379288164665525 37.29974271012007 16.752572898799315 52-53 13.08983704974271 34.11170668953688 36.27594339622642 16.522512864493997 54-55 13.010291595197254 33.50686106346484 35.20690394511149 18.275943396226417 56-57 14.161449399656947 33.921740994854204 33.61942538593482 18.29738421955403 58-59 13.533233276157805 34.151801029159515 34.577186963979415 17.737778730703262 60-61 14.679030874785592 35.114493996569465 33.31882504288164 16.88765008576329 62-63 14.501500857632932 35.382718696397944 33.24678387650086 16.868996569468266 64-65 14.673241852487134 35.58147512864494 33.374785591766724 16.3704974271012 66-67 14.931421379548413 35.04488645987663 33.0152937720707 17.008398388504265 68-69 15.105521238253083 34.303890016916846 33.23634913453931 17.354239610290758 70-71 14.883790737564324 34.19275300171527 33.328259005145796 17.59519725557461 72-73 14.754931389365353 33.262221269296745 33.438893653516296 18.543953687821613 74-75 14.021440823327616 33.72984562607204 33.04009433962264 19.2086192109777 76-77 13.156303602058319 33.07611492281304 34.72341337907376 19.04416809605489 78-79 12.777444253859349 32.91445111492281 34.86234991423671 19.44575471698113 80-81 12.77556936350499 32.360130189237516 34.79903430117625 20.065266146081242 82-83 12.62114272972092 32.20359697421911 34.07048148338737 21.1047788126726 84-85 13.161556752955203 31.67603311455655 33.476277978734124 21.68613215375412 86-87 12.971193945046581 31.692020712057378 33.524587527739364 21.81219781515668 88-89 13.19039451114923 31.293953687821613 33.10077186963979 22.414879931389365 90-91 13.34464023054063 31.04558066134628 33.595421778713586 22.014357329399505 92-93 13.386392337890948 31.060315309423903 33.62507209492301 21.928220257762128 94-95 13.63271869639794 30.65415951972556 33.46740994854203 22.24571183533448 96-97 13.900329974978506 30.73291323523421 33.3041022815229 22.062654508264384 98-99 14.12843053173242 30.64022298456261 33.00643224699829 22.224914236706688 100-101 14.465547703180212 30.065353871487872 33.44818861710522 22.020909808226698 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7274.0 1 4487.0 2 2006.0 3 2201.0 4 1619.0 5 1467.5 6 2826.5 7 5622.0 8 7759.0 9 7674.0 10 5241.0 11 2803.0 12 2377.5 13 2340.5 14 2161.0 15 1905.5 16 1664.0 17 1542.5 18 1467.0 19 1429.0 20 1429.0 21 1439.0 22 1493.0 23 1593.0 24 1680.0 25 1935.5 26 2431.5 27 2935.0 28 3210.0 29 3406.5 30 3915.5 31 4462.0 32 4901.5 33 5319.0 34 5894.5 35 6373.0 36 6299.5 37 6264.5 38 6048.5 39 5412.0 40 5033.0 41 5078.0 42 5176.0 43 5325.0 44 5537.5 45 5328.0 46 4897.5 47 4935.0 48 5518.5 49 5414.0 50 4932.5 51 4540.5 52 3798.5 53 2979.0 54 2348.5 55 2246.0 56 2532.0 57 2762.0 58 2520.0 59 2517.0 60 2808.0 61 3005.0 62 2471.0 63 1088.5 64 292.5 65 167.5 66 138.0 67 119.5 68 97.0 69 102.0 70 103.5 71 108.5 72 105.0 73 122.0 74 150.5 75 102.5 76 47.0 77 34.0 78 29.0 79 13.0 80 3.0 81 1.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014150943396226415 3 0.005574614065180103 4 0.0 5 4.288164665523156E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.144082332761578E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012864493996569467 26-27 0.023799313893653515 28-29 2.144082332761578E-4 30-31 0.0 32-33 0.005574614065180103 34-35 0.016080617495711838 36-37 0.009433962264150943 38-39 0.02444253859348199 40-41 0.024656946826758147 42-43 0.020154373927958835 44-45 0.01179245283018868 46-47 0.0025728987993138934 48-49 6.432246998284733E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.144082332761578E-4 68-69 2.144082332761578E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.288164665523156E-4 82-83 0.0017152658662092624 84-85 0.004073756432246998 86-87 0.0010720411663807888 88-89 0.0 90-91 0.0038593481989708405 92-93 2.144082332761578E-4 94-95 0.0 96-97 2.144082332761578E-4 98-99 0.0 100-101 0.003430531732418525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 233200.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.485420240137216 #Duplication Level Percentage of deduplicated Percentage of total 1 78.35049249008334 30.153516295025728 2 11.077684182377324 8.526586620926244 3 4.023487988590275 4.645368782161235 4 1.8763649329232963 2.888507718696398 5 1.0429201764941836 2.0068610634648367 6 0.6997370414939609 1.6157804459691252 7 0.4735481570619958 1.275728987993139 8 0.3454115969158087 1.0634648370497428 9 0.2562731202923742 0.8876500857632932 >10 1.5443241075010028 11.459262435677529 >50 0.18050541516245489 4.975128644939965 >100 0.1103088648215002 7.671526586620926 >500 0.005571154788964657 1.3083190394511148 >1k 0.00891384766234345 6.377787307032589 >5k 0.003342692873378794 10.380789022298456 >10k+ 0.0011142309577929312 4.7637221269296735 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11109 4.7637221269296735 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9512 4.078902229845626 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7895 3.3855060034305313 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6801 2.9163807890222984 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2653 1.1376500857632932 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2496 1.0703259005145798 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 2424 1.039451114922813 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1857 0.79631217838765 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1638 0.7024013722126929 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 1462 0.6269296740994854 RNA PCR Primer, Index 23 (95% over 23bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1230 0.5274442538593482 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1113 0.4772727272727273 No Hit ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 794 0.3404802744425386 RNA PCR Primer, Index 23 (96% over 25bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 670 0.28730703259005147 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 555 0.23799313893653518 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 525 0.2251286449399657 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 507 0.217409948542024 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 437 0.1873927958833619 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 435 0.1865351629502573 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 392 0.1680960548885077 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 388 0.16638078902229844 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 387 0.16595197255574615 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 377 0.161663807890223 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 375 0.16080617495711835 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 364 0.15608919382504288 No Hit CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT 349 0.14965694682675815 RNA PCR Primer, Index 23 (96% over 28bp) TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC 339 0.145368782161235 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 324 0.13893653516295024 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 324 0.13893653516295024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 315 0.1350771869639794 No Hit TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 310 0.13293310463121785 RNA PCR Primer, Index 23 (96% over 30bp) CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 298 0.12778730703259006 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 282 0.12092624356775301 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 278 0.11921097770154375 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 277 0.11878216123499141 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 264 0.11320754716981132 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 252 0.10806174957118354 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 247 0.10591766723842194 No Hit CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG 239 0.10248713550600343 Illumina PCR Primer Index 1 (95% over 23bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.288164665523156E-4 0.0 20-21 0.0 0.0 0.0 0.00192967409948542 0.0 22-23 0.0 0.0 0.0 0.011363636363636364 0.0 24-25 0.0 0.0 0.0 0.03473413379073756 0.0 26-27 0.0 0.0 0.0 0.0756861063464837 0.0 28-29 0.0 0.0 0.0 0.16916809605488853 0.0 30-31 0.0 0.0 0.0 0.3850771869639794 0.0 32-33 0.0 0.0 0.0 0.6183533447684391 0.0 34-35 0.0 0.0 0.0 0.8737135506003431 0.0 36-37 0.0 0.0 0.0 1.1790308747855918 0.0 38-39 0.0 0.0 0.0 1.5752572898799313 0.0 40-41 0.0 0.0 0.0 2.0034305317324184 0.0 42-43 0.0 0.0 0.0 2.342838765008576 0.0 44-45 0.0 0.0 0.0 2.664236706689537 0.0 46-47 0.0 0.0 0.0 3.007933104631218 0.0 48-49 0.0 0.0 0.0 3.353130360205832 0.0 50-51 0.0 0.0 0.0 3.732204116638079 0.0 52-53 0.0 0.0 0.0 4.051672384219554 0.0 54-55 0.0 0.0 0.0 4.346483704974271 0.0 56-57 0.0 0.0 0.0 4.64622641509434 0.0 58-59 0.0 0.0 0.0 4.943181818181818 0.0 60-61 0.0 0.0 0.0 5.282161234991424 0.0 62-63 0.0 0.0 0.0 5.612564322469983 0.0 64-65 0.0 0.0 0.0 5.902873070325901 0.0 66-67 0.0 0.0 0.0 6.215480274442539 0.0 68-69 0.0 0.0 0.0 6.529802744425386 0.0 70-71 0.0 0.0 0.0 6.89901372212693 0.0 72-73 0.0 0.0 0.0 7.233061749571183 0.0 74-75 0.0 0.0 0.0 7.566252144082332 0.0 76-77 0.0 0.0 0.0 7.8647084048027445 0.0 78-79 0.0 0.0 0.0 8.214622641509434 0.0 80-81 0.0 0.0 0.0 8.569039451114921 0.0 82-83 0.0 0.0 0.0 8.935463121783876 0.0 84-85 0.0 0.0 0.0 9.28795025728988 0.0 86-87 0.0 0.0 0.0 9.626072041166381 0.0 88-89 0.0 0.0 0.0 9.984777015437391 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTATC 25 4.5918932E-7 95.05943 2 CGTTGCA 25 0.0050166296 56.99897 9 GGTATCA 3690 0.0 51.007496 1 GCGGTAT 60 1.4807338E-6 47.529716 1 GGATGGC 50 0.0016133183 37.999313 8 CCACGCG 25 0.0016149746 37.999313 88-89 TACCTGG 65 1.3200719E-4 36.561317 2 GGGAATG 105 4.631147E-8 36.189823 7 CGTCACT 80 1.069336E-5 35.647285 1 GTATCAA 5835 0.0 34.94473 1 TGGGGAT 160 5.2750693E-11 32.65566 6 ATGGGGT 220 0.0 32.38578 5 GTGTAGC 60 0.00393249 31.686478 1 GTCACTA 110 2.707904E-6 30.246181 2 GAATGCA 110 2.72153E-6 30.226728 9 TGTAGAC 80 4.466388E-4 29.706072 2 TGGGAGT 160 1.88993E-9 29.686962 6 CCTGGCT 85 6.392431E-4 27.940674 8 TATCAAC 7480 0.0 27.196148 2 ACCAGGC 70 0.008378839 27.142366 7 >>END_MODULE