##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938273_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 237458 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3753168981462 33.0 31.0 34.0 30.0 34.0 2 31.4259237423039 33.0 31.0 34.0 30.0 34.0 3 31.337049078152766 33.0 31.0 34.0 28.0 34.0 4 34.92464351590597 37.0 35.0 37.0 33.0 37.0 5 34.984494100009265 37.0 35.0 37.0 33.0 37.0 6 35.14392018799114 37.0 35.0 37.0 33.0 37.0 7 35.10363095789571 37.0 35.0 37.0 33.0 37.0 8 35.107597975220884 37.0 35.0 37.0 33.0 37.0 9 36.63941833924315 39.0 37.0 39.0 33.0 39.0 10-11 36.58758180394007 39.0 37.0 39.0 33.0 39.0 12-13 36.459725509353234 39.0 37.0 39.0 32.5 39.0 14-15 37.29373194417539 40.0 37.5 41.0 32.0 41.0 16-17 37.298425405755964 40.0 37.5 41.0 32.5 41.0 18-19 37.44641157594185 40.0 37.5 41.0 33.0 41.0 20-21 37.5168071827439 40.0 37.5 41.0 33.0 41.0 22-23 37.33031736138601 40.0 36.5 41.0 33.0 41.0 24-25 37.105673424352936 40.0 36.0 41.0 33.0 41.0 26-27 36.83420857583236 40.0 35.0 41.0 32.5 41.0 28-29 36.524728583581094 39.5 35.0 41.0 32.0 41.0 30-31 35.795820734614125 38.0 35.0 40.5 31.0 41.0 32-33 34.9588495649757 36.0 35.0 40.0 30.5 41.0 34-35 34.460245601327394 35.0 35.0 40.0 29.0 41.0 36-37 34.00712547060954 35.0 35.0 40.0 25.0 41.0 38-39 33.49920828104338 35.0 35.0 40.0 22.0 41.0 40-41 32.90459786572784 35.0 35.0 40.0 13.5 41.0 42-43 32.08469497763815 35.0 33.5 39.0 2.0 41.0 44-45 30.915968297551565 35.0 33.0 39.0 2.0 41.0 46-47 29.560791803182035 35.0 30.0 39.0 2.0 41.0 48-49 28.580557403835627 35.0 23.5 39.0 2.0 41.0 50-51 27.65797530510659 35.0 21.0 38.0 2.0 40.5 52-53 27.5151900546623 35.0 20.0 38.0 2.0 40.0 54-55 27.392465193844806 35.0 10.0 38.0 2.0 41.0 56-57 27.045995502362523 35.0 2.0 38.0 2.0 41.0 58-59 26.714381069494394 35.0 2.0 37.5 2.0 41.0 60-61 26.322273833688485 35.0 2.0 37.0 2.0 40.5 62-63 25.90229008919472 35.0 2.0 36.0 2.0 40.0 64-65 25.480345998029126 35.0 2.0 35.5 2.0 40.0 66-67 25.05415905128486 35.0 2.0 35.0 2.0 39.5 68-69 24.602498125984383 34.5 2.0 35.0 2.0 39.0 70-71 24.19154124097735 34.0 2.0 35.0 2.0 39.0 72-73 23.777625938060623 34.0 2.0 35.0 2.0 38.0 74-75 23.343157526804738 33.0 2.0 35.0 2.0 37.0 76-77 22.91174228705708 33.0 2.0 35.0 2.0 37.0 78-79 22.44116433221875 33.0 2.0 35.0 2.0 36.0 80-81 21.965596021191118 32.5 2.0 35.0 2.0 36.0 82-83 21.48603121394099 31.5 2.0 35.0 2.0 35.0 84-85 21.03422710542496 31.0 2.0 35.0 2.0 35.0 86-87 20.566746540440835 30.5 2.0 35.0 2.0 35.0 88-89 20.074697420175355 29.5 2.0 35.0 2.0 35.0 90-91 19.53267104077353 29.0 2.0 35.0 2.0 35.0 92-93 19.003668017080916 27.0 2.0 35.0 2.0 35.0 94-95 18.601068399464324 25.5 2.0 35.0 2.0 35.0 96-97 18.023465202267346 23.5 2.0 34.0 2.0 35.0 98-99 17.367749244076848 10.5 2.0 34.0 2.0 35.0 100-101 15.856408291150434 2.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.058723913081635715 1101 2 -0.16916409051395576 1101 3 -0.2199496328039885 1101 4 -0.28097938038272474 1101 5 -0.5182804889180019 1101 6 -0.4809035379530471 1101 7 -0.2948028322313121 1101 8 -0.2798993234440772 1101 9 -0.8951906148971318 1101 10-11 -0.2622186585182149 1101 12-13 -0.1380237794526522 1101 14-15 -0.872853605971919 1101 16-17 -0.43631921349845015 1101 18-19 -0.31165094960199724 1101 20-21 -0.17771222161790234 1101 22-23 0.02199665792497285 1101 24-25 -0.03968909599144155 1101 26-27 -0.2410832722433227 1101 28-29 -0.5088621239760798 1101 30-31 -0.4527085981993153 1101 32-33 -0.3586337919713216 1101 34-35 -0.28896156074242185 1101 36-37 -0.32840060079481503 1101 38-39 -0.658500585732817 1101 40-41 -0.798253940422434 1101 42-43 -0.9164999313278486 1101 44-45 -0.5175583779083546 1101 46-47 0.05844778045674559 1101 48-49 0.08414659369038446 1101 50-51 -0.033251992261639884 1101 52-53 -0.026697755230159714 1101 54-55 0.14425242655591575 1101 56-57 -0.11346070241274475 1101 58-59 0.15789394245083344 1101 60-61 -0.1846827331327816 1101 62-63 0.25899980320443916 1101 64-65 0.13919137904211 1101 66-67 -0.21020474425910507 1101 68-69 -0.38416084566373954 1101 70-71 -0.560080317177178 1101 72-73 -0.38121339002169563 1101 74-75 -0.1917467579478327 1101 76-77 -0.5423013350579957 1101 78-79 -0.8990231247305864 1101 80-81 -0.36006716363084124 1101 82-83 -0.7532752511374241 1101 84-85 -0.8951721729039761 1101 86-87 -0.769600141678243 1101 88-89 -0.517181024717285 1101 90-91 -0.15599714785800955 1101 92-93 0.09639411735729553 1101 94-95 0.2576505324454317 1101 96-97 0.27480865179884617 1101 98-99 0.16759035198155203 1101 100-101 -0.22621418288074402 1102 1 0.03536558874780482 1102 2 -0.22151421716748843 1102 3 -0.10703097251690963 1102 4 -0.3977257918659802 1102 5 0.13685601544450776 1102 6 -0.33958634121789544 1102 7 -0.5979550979222807 1102 8 -0.21496827925606965 1102 9 -0.12372987749211717 1102 10-11 -0.27903543307492384 1102 12-13 -0.14999957455538038 1102 14-15 -0.2070219144436365 1102 16-17 -0.4143377893498439 1102 18-19 -0.6782284897089497 1102 20-21 -0.6713091819331254 1102 22-23 -0.73764308313892 1102 24-25 -0.2582227295405559 1102 26-27 6.983207766566579E-4 1102 28-29 0.1526070569977449 1102 30-31 -0.3174848436257136 1102 32-33 -0.5667677632631936 1102 34-35 -0.9059612792901319 1102 36-37 -0.7874211468122638 1102 38-39 -0.4228124348743876 1102 40-41 -0.13613741917279043 1102 42-43 0.13420369940673993 1102 44-45 0.07454688524953923 1102 46-47 0.27290035574523586 1102 48-49 0.3649093294066823 1102 50-51 0.11940773191511056 1102 52-53 -0.26080977324310695 1102 54-55 -0.6164286879951639 1102 56-57 -1.306959154425126 1102 58-59 -1.356150527011593 1102 60-61 -1.3975591755757897 1102 62-63 -1.214529608560266 1102 64-65 -0.936125254788454 1102 66-67 -0.6203933172959744 1102 68-69 -0.41061736128434134 1102 70-71 -0.29859706358866234 1102 72-73 -0.5481427454959409 1102 74-75 -0.7705393276072741 1102 76-77 -0.771628664075724 1102 78-79 -0.42073716920004856 1102 80-81 -0.42481526382785617 1102 82-83 -0.8067652595809918 1102 84-85 -0.7237958711871144 1102 86-87 -0.4821247687539554 1102 88-89 -0.5571050325979492 1102 90-91 -0.4537033116632472 1102 92-93 -0.6503128908048232 1102 94-95 -1.7744772468959695 1102 96-97 -1.2638628933742453 1102 98-99 -0.7379542582070222 1102 100-101 -0.7072583708908748 1103 1 0.2979090031213012 1103 2 0.2615207814966851 1103 3 -7.525861957766722E-4 1103 4 0.36230626797906496 1103 5 0.09552553187518242 1103 6 0.437036688437459 1103 7 0.7373641647012832 1103 8 0.3095575022026509 1103 9 0.7643278302289573 1103 10-11 0.603345010419595 1103 12-13 0.3936953225859554 1103 14-15 0.36078465782460256 1103 16-17 0.25448401134478615 1103 18-19 0.07583242399662993 1103 20-21 0.8031097333754431 1103 22-23 0.5527656798853968 1103 24-25 0.6418440618032903 1103 26-27 0.8357103432185582 1103 28-29 0.7293636693407777 1103 30-31 1.0277061796176739 1103 32-33 0.47223838154044273 1103 34-35 0.06163156067781017 1103 36-37 0.48693097715247546 1103 38-39 0.800564535470258 1103 40-41 0.994506449381845 1103 42-43 1.3607198629119566 1103 44-45 0.5332030434114436 1103 46-47 0.515232709472194 1103 48-49 0.8387137643519154 1103 50-51 1.203725124381048 1103 52-53 1.0590673306842255 1103 54-55 0.8180356454272228 1103 56-57 0.6028725313883925 1103 58-59 0.8149421793736593 1103 60-61 1.1394031541062866 1103 62-63 1.14105414778491 1103 64-65 1.2549247051367374 1103 66-67 1.204586645300875 1103 68-69 1.4157251447268777 1103 70-71 1.2890198212630608 1103 72-73 1.6887260763047536 1103 74-75 1.7867542871402549 1103 76-77 1.5732284024484535 1103 78-79 1.4812532128464362 1103 80-81 1.5121470658542187 1103 82-83 1.7295825897377348 1103 84-85 1.375749947674759 1103 86-87 1.303501734051281 1103 88-89 1.5236724186443702 1103 90-91 1.2743891275566313 1103 92-93 1.2389604193741803 1103 94-95 1.2584241690805165 1103 96-97 0.7520067299225737 1103 98-99 0.7851288302247177 1103 100-101 1.134219053634908 1104 1 0.9129161000972701 1104 2 0.9560861819345305 1104 3 0.7167155977001052 1104 4 0.7547810677000015 1104 5 0.8646933813711755 1104 6 0.7551255570110911 1104 7 0.7630576234546211 1104 8 0.5243432697586456 1104 9 0.780735076361708 1104 10-11 0.9815657143433825 1104 12-13 0.6856576965845562 1104 14-15 0.6606258780233176 1104 16-17 0.7315090552498162 1104 18-19 0.9859895197697242 1104 20-21 1.0485087277700416 1104 22-23 1.0591890445737278 1104 24-25 1.2414904158822466 1104 26-27 0.9295708575688764 1104 28-29 1.0856078053140337 1104 30-31 1.0948748470702228 1104 32-33 0.8837186423242329 1104 34-35 0.9392869122788383 1104 36-37 0.9739158449562595 1104 38-39 1.1436131076549216 1104 40-41 0.9525380609968366 1104 42-43 1.1217442332056962 1104 44-45 1.247844465693536 1104 46-47 0.4706179910657653 1104 48-49 0.6825321779973521 1104 50-51 0.9835384378268124 1104 52-53 0.6991303614538253 1104 54-55 0.6549856297898167 1104 56-57 0.4973561361710317 1104 58-59 0.0022181434798014266 1104 60-61 0.06379028813087118 1104 62-63 -0.32756278391572025 1104 64-65 -0.28962986940556945 1104 66-67 -0.39497550736831144 1104 68-69 -0.323563233072818 1104 70-71 -0.32569858516511374 1104 72-73 -0.4510362351442332 1104 74-75 -0.7972666910395496 1104 76-77 -0.6161285393563425 1104 78-79 -0.6099489427096536 1104 80-81 -0.7317122456188265 1104 82-83 -1.0438193877925173 1104 84-85 -0.7909447861284988 1104 86-87 -0.6110097774843091 1104 88-89 -0.6412781430993206 1104 90-91 -0.6252548207677613 1104 92-93 -0.6381776949953917 1104 94-95 -0.7391941339002095 1104 96-97 -0.5464271238556648 1104 98-99 -1.1377547071967946 1104 100-101 -0.6536415088305105 1105 1 0.4273048964765849 1105 2 0.2585711313389112 1105 3 0.29192587907152046 1105 4 0.42868482928148666 1105 5 0.26198640899595205 1105 6 0.3025189219399991 1105 7 0.0010491695030410142 1105 8 -0.07300526360884163 1105 9 0.2432687000327718 1105 10-11 -0.054758524587498414 1105 12-13 -0.08567999181474306 1105 14-15 -0.016576205576875225 1105 16-17 0.14052233393133662 1105 18-19 0.1249246634442045 1105 20-21 0.3291649764357629 1105 22-23 0.3303986427975474 1105 24-25 0.39011771616831226 1105 26-27 0.39157077217542735 1105 28-29 0.6522040223841756 1105 30-31 0.584435497768311 1105 32-33 0.3784669451294107 1105 34-35 0.34159680511005774 1105 36-37 0.35930716044236277 1105 38-39 0.30895617584159396 1105 40-41 0.3227293423255517 1105 42-43 0.22941469988089835 1105 44-45 -0.36440522475520254 1105 46-47 -0.6793756044254593 1105 48-49 -0.4067360001333853 1105 50-51 -0.2296676592655409 1105 52-53 -0.08577421136543961 1105 54-55 -0.4421280715892877 1105 56-57 -0.8368311316655266 1105 58-59 -1.1032107451244428 1105 60-61 -0.8019925563249579 1105 62-63 -1.215430509461168 1105 64-65 -1.3538350698666939 1105 66-67 -1.0446702257834097 1105 68-69 -1.2894698980316157 1105 70-71 -1.1233481304449917 1105 72-73 -1.1155680655528393 1105 74-75 -1.3508617552981192 1105 76-77 -1.1924916323071137 1105 78-79 -1.2382099050938393 1105 80-81 -1.3977171130454948 1105 82-83 -1.546381191302185 1105 84-85 -1.4962946857911952 1105 86-87 -1.3240877844011791 1105 88-89 -1.2731790013034967 1105 90-91 -1.510720855522898 1105 92-93 -1.3932479311082844 1105 94-95 -1.00909080782532 1105 96-97 -0.7599747947493043 1105 98-99 -1.3930277527542003 1105 100-101 -1.11126500910804 1106 1 0.3965409486722784 1106 2 0.6812471706068806 1106 3 0.486124562059441 1106 4 0.6419721075911866 1106 5 0.2786308561336739 1106 6 0.5289763210431389 1106 7 0.3321605499417757 1106 8 0.38845395345575184 1106 9 0.7950806016204623 1106 10-11 0.4447667854759345 1106 12-13 0.5444658490934273 1106 14-15 0.6567260204159382 1106 16-17 0.965553643267576 1106 18-19 0.5906055910085399 1106 20-21 0.7218345518407645 1106 22-23 0.47500737709237484 1106 24-25 0.48158137713870275 1106 26-27 0.3878944501134569 1106 28-29 0.15772860526302424 1106 30-31 0.16078987905542164 1106 32-33 -0.02251475405856951 1106 34-35 -0.13990038714946706 1106 36-37 -0.1919290533499023 1106 38-39 -0.5604333930995509 1106 40-41 -0.6250446648654915 1106 42-43 -0.7971982359248599 1106 44-45 -0.0893072568321216 1106 46-47 0.562716263226946 1106 48-49 0.4278194072919774 1106 50-51 0.4356456360925982 1106 52-53 1.050542598718188 1106 54-55 1.4363917037914753 1106 56-57 1.0359721649045888 1106 58-59 0.7429998152116788 1106 60-61 0.7038451199163056 1106 62-63 1.0969053690182058 1106 64-65 1.447613239429149 1106 66-67 1.183382939489487 1106 68-69 1.1771806089776327 1106 70-71 1.5685377015753694 1106 72-73 1.9210335584927272 1106 74-75 1.9062484939645934 1106 76-77 2.0883430139983794 1106 78-79 1.7735866448193 1106 80-81 1.4398177312158076 1106 82-83 1.3881721961659998 1106 84-85 1.4870608670710865 1106 86-87 2.2727489626597794 1106 88-89 1.9535510920185253 1106 90-91 1.1723882625637998 1106 92-93 0.7847708948926027 1106 94-95 0.8484517122732562 1106 96-97 1.2561040643788814 1106 98-99 1.2700938890669953 1106 100-101 0.8936030876883052 1107 1 0.6000023873558931 1107 2 0.4161543343766603 1107 3 0.1312900366174965 1107 4 0.3057974791214093 1107 5 0.4943592777324568 1107 6 0.5669817318573536 1107 7 0.8099613875061706 1107 8 0.5596728690692885 1107 9 0.557653332599223 1107 10-11 0.7216170245153251 1107 12-13 0.6558507951705934 1107 14-15 0.46866316602613267 1107 16-17 0.45308282831001634 1107 18-19 0.4511103532662588 1107 20-21 0.8386951677841381 1107 22-23 0.7351711839337227 1107 24-25 0.7059356001680115 1107 26-27 0.9085278118597415 1107 28-29 1.2622851779198854 1107 30-31 0.9358426900845913 1107 32-33 1.1103744724914861 1107 34-35 1.1784667328890777 1107 36-37 1.4625571046013945 1107 38-39 1.5363219713892065 1107 40-41 1.7191257387219494 1107 42-43 1.753407701146628 1107 44-45 1.680788729529656 1107 46-47 1.548846419251106 1107 48-49 1.0813512606811493 1107 50-51 0.48230464361585135 1107 52-53 0.6971719579443665 1107 54-55 0.7281124168330173 1107 56-57 0.9635932596679595 1107 58-59 0.9281904908222494 1107 60-61 0.5700571793611395 1107 62-63 0.3817513831752706 1107 64-65 0.32574947335421456 1107 66-67 0.5818467870972412 1107 68-69 0.13722952866780957 1107 70-71 0.6362006745583564 1107 72-73 0.7800651709894879 1107 74-75 1.0260461443370517 1107 76-77 1.1216510227446292 1107 78-79 0.7498587420657152 1107 80-81 0.7733796035057701 1107 82-83 0.41894156947511973 1107 84-85 0.333315916546681 1107 86-87 0.05769689283804169 1107 88-89 0.3124251979370669 1107 90-91 0.35980256045506565 1107 92-93 0.185115556346112 1107 94-95 -0.18826585063672852 1107 96-97 -0.2219098703207436 1107 98-99 -0.4032652629917095 1107 100-101 -0.271547192117497 1108 1 -0.5590717240463761 1108 2 -0.643208697270186 1108 3 -0.5440442373863981 1108 4 -0.6423984491188648 1108 5 -0.6726433427069693 1108 6 -0.5552046552957748 1108 7 -0.5635520170493677 1108 8 -1.021258394788802 1108 9 -0.9750349694904088 1108 10-11 -0.7764680389799281 1108 12-13 -0.8944582823003486 1108 14-15 -0.9453830612130005 1108 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-0.22656785005841584 2316 92-93 -0.5081644870084467 2316 94-95 -0.4131743996138333 2316 96-97 -0.12029084850798455 2316 98-99 -0.08739342990676136 2316 100-101 -0.10108925958802928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4848.0 3 681.0 4 539.0 5 470.0 6 459.0 7 464.0 8 644.0 9 723.0 10 963.0 11 1371.0 12 2247.0 13 3445.0 14 3963.0 15 5636.0 16 8174.0 17 11689.0 18 8777.0 19 4626.0 20 4012.0 21 3652.0 22 3526.0 23 3584.0 24 3564.0 25 3724.0 26 3764.0 27 4048.0 28 4450.0 29 4907.0 30 5580.0 31 6339.0 32 7538.0 33 9473.0 34 14353.0 35 33390.0 36 23949.0 37 18575.0 38 16956.0 39 2355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.59323009467541 21.155742526906522 15.563248843537991 11.68777853488008 2 17.189218342469736 26.460735940403406 38.776550695448094 17.573495021678767 3 19.25407473579531 36.310236140841745 31.579834482293652 12.855854641069293 4 10.726251332774234 23.653213197520305 34.7823521726854 30.838183297020066 5 8.921925250898852 43.51119297609664 28.99678394247346 18.570097830531047 6 20.89189804470451 41.42840279592921 17.15457711149147 20.52512204787481 7 19.86041277695125 46.52780997256302 18.19017073304365 15.421606517442079 8 27.5885317467006 32.00247770904617 17.465741880024947 22.943248664228285 9 31.235331049506787 17.847135314616068 19.306761727786416 31.61077190809073 10-11 23.847697080045858 33.26041877402387 20.64556780632544 22.246316339604828 12-13 29.382872200263506 34.10508563899868 23.722793148880108 12.789249011857706 14-15 24.65040821701343 31.72188569923624 30.647985251514353 12.979720832235975 16-17 14.704159590741108 36.088273839372626 29.89397700025705 19.313589569629215 18-19 13.89637451987198 29.110032805675363 42.511267074437484 14.48232560001517 20-21 17.89746086135574 26.772750357721627 43.46733933083403 11.862449450088613 22-23 16.68998474491989 28.904162698379253 42.94431474348709 11.461537813213765 24-25 14.36155415430267 30.02427839223091 43.90068620178042 11.713481251686 26-27 13.59596444871487 30.566688159264366 44.16307403146348 11.67427336055729 28-29 13.248206327633616 31.350759084252555 43.74600967160783 11.655024916506001 30-31 13.314424310877815 31.78422641938835 43.24912075152828 11.652228518205554 32-33 12.144199728592982 32.34400418067953 43.43439451791539 12.077401572812096 34-35 12.289566901341672 32.61104566996167 43.238641511978535 11.860745916718118 36-37 12.720698396242517 32.423884207487575 42.321834575502606 12.5335828207673 38-39 12.434196251582556 33.033429041492965 42.10660470563948 12.425770001285002 40-41 12.103286206947402 33.27055433017121 41.89887524463798 12.72728421824341 42-43 12.008872991095409 33.15385117727234 41.378010065325604 13.45926576630665 44-45 12.622551342562463 32.92347741474849 40.69578623465409 13.758185008034946 46-47 12.771926868312411 33.380444856348475 39.503117364563145 14.34451091077597 48-49 12.753597546591177 33.40621103359957 38.12704310315775 15.713148316651502 50-51 13.169102190918702 33.86614375560789 36.67735236257788 16.287401690895535 52-53 13.602788630643344 34.15440673146793 35.517128805669934 16.725675832218794 54-55 13.633424531739363 33.301108492404 35.31526636042537 17.750200615431275 56-57 14.342612699328743 33.42993927726678 34.30971332045722 17.917734702947254 58-59 14.581328098837613 32.85832531239535 34.58347637631925 17.976870212447793 60-61 15.285764235553815 32.94808767340267 34.69738521617147 17.06876287487204 62-63 15.810457323622702 32.64743916462724 34.90078052931092 16.641322982439142 64-65 16.15635727975401 32.99109117331143 34.745055917103684 16.10749562983088 66-67 16.373304692106817 32.512846432482526 34.53773902788308 16.576109847527587 68-69 16.256720982100546 32.17626084887083 34.977960022661264 16.589058146367353 70-71 16.08163420075289 32.31641679113045 34.96796944615896 16.633979561957688 72-73 15.98100799197853 31.62097715313676 35.193353639783794 17.204661215100923 74-75 14.941010685691536 32.22432158602371 35.34703747469705 17.4876302535877 76-77 14.26263689974726 31.674178601516427 35.96377422072452 18.099410278011792 78-79 14.133390055029874 31.480120379164884 36.05730856953919 18.329180996266057 80-81 14.408885669036628 30.933256393840345 35.75390875088452 18.9039491862385 82-83 14.559362764610858 30.86050787593118 34.951484937900304 19.628644421557663 84-85 15.358889361576662 30.187057995981686 34.734829179531026 19.719223462910623 86-87 15.272387266061152 30.184876710251718 34.40720048351398 20.135535540173148 88-89 15.619950832829513 29.683362579443738 34.31253383666135 20.384152751065393 90-91 16.1996237251213 29.533996414222603 34.12029419386373 20.146085666792374 92-93 16.78918987235118 29.157222900956313 34.47550238025025 19.578084846442263 94-95 17.28375554094824 28.451399774148424 34.62249919940672 19.64234548549662 96-97 17.500426587907654 28.21968539931788 34.502851292508694 19.77703672026577 98-99 18.001356526281644 28.040072291897495 34.10547202480505 19.853099157015812 100-101 19.363819864968328 27.05843146436172 34.463237511890675 19.114511158779273 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8028.0 1 4888.5 2 2153.0 3 2370.5 4 1700.5 5 1522.0 6 2782.0 7 5516.5 8 8014.5 9 7967.0 10 5257.0 11 2897.0 12 2440.0 13 2366.5 14 2238.5 15 1977.0 16 1718.5 17 1560.0 18 1500.0 19 1511.5 20 1530.5 21 1584.5 22 1650.0 23 1690.5 24 1807.5 25 2101.0 26 2570.5 27 3062.0 28 3313.0 29 3598.5 30 4059.5 31 4508.0 32 4886.5 33 5289.5 34 5893.5 35 6269.0 36 6160.0 37 6175.0 38 5906.0 39 5187.0 40 4777.5 41 4681.0 42 4758.5 43 4795.5 44 4727.5 45 4527.5 46 4284.0 47 4476.0 48 5259.5 49 5405.5 50 4950.0 51 4365.0 52 3707.0 53 3347.5 54 2936.5 55 2757.0 56 2956.0 57 3051.0 58 2833.5 59 2797.0 60 3057.5 61 3357.5 62 2815.5 63 1388.0 64 543.5 65 395.5 66 348.5 67 322.0 68 290.5 69 258.5 70 248.0 71 247.5 72 219.5 73 220.5 74 214.0 75 149.0 76 92.0 77 71.0 78 63.5 79 39.5 80 23.5 81 17.0 82 16.5 83 11.5 84 8.0 85 5.0 86 1.5 87 0.5 88 1.5 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49566660209384394 2 0.054325396491168965 3 0.05980004885074413 4 0.07201273488364257 5 0.08885781906695078 6 0.10780853877317251 7 0.07875076855696586 8 0.05980004885074413 9 0.0576944133278306 10-11 0.0816986582890448 12-13 0.11391488178962175 14-15 0.061273993716783604 16-17 0.06359019279198848 18-19 0.06380075634427983 20-21 0.08106696763217074 22-23 0.06738033673323282 24-25 0.08843669196236809 26-27 0.07032822646531177 28-29 0.06969653580843771 30-31 0.07643456948176099 32-33 0.0741183704065561 34-35 0.07559231527259558 36-37 0.07222329843593393 38-39 0.04400778242889269 40-41 0.04948243478846786 42-43 0.04569229084722351 44-45 0.024214808513505548 46-47 0.032847914157451 48-49 0.03116340573912018 50-51 0.029057770216206653 52-53 0.02758382535016719 54-55 0.02674157114100178 56-57 0.028426079559332598 58-59 0.024635935618088252 60-61 0.0324267870528683 62-63 0.023161990752048785 64-65 0.022319736542883373 66-67 0.016002829974142797 68-69 0.0204246645722612 70-71 0.0448500366380581 72-73 0.04000707493535699 74-75 0.03558524033723859 76-77 0.0244253720657969 78-79 0.01747677484018226 80-81 0.017687338392473616 82-83 0.024214808513505548 84-85 0.01895071970622173 86-87 0.012423249585189804 88-89 0.04400778242889269 90-91 0.05537821425262572 92-93 0.03705918520327805 94-95 0.05643103201408249 96-97 0.046113417951806214 98-99 0.03663805809869535 100-101 0.16950365959453884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 237458.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.549663519443435 #Duplication Level Percentage of deduplicated Percentage of total 1 80.47900428732149 33.438755485180536 2 10.012872099976688 8.320629332345089 3 3.55046978097159 4.425624742059648 4 1.763579051924227 2.931044647895628 5 0.9578058644071232 1.9898255691532818 6 0.6365101405795486 1.5868069300676328 7 0.3841358969421161 1.1172502084579168 8 0.33041768444097586 1.098299488751695 9 0.22602191297649574 0.8452020988974893 >10 1.3946464226711128 11.262623284959867 >50 0.14899202335221917 4.3245542369597985 >100 0.1013551179266797 7.243807325927111 >500 0.004054204717067188 1.0132318136259886 >1k 0.006081307075600783 4.795374339883264 >5k 0.0030406535378003914 10.57365934186256 >10k+ 0.001013551179266797 5.033311153972492 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11952 5.033311153972492 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9614 4.048715983458127 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8644 3.640222692012903 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6850 2.88472066639153 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2941 1.2385348145777357 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2369 0.9976501107564284 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1936 0.8153020744721172 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1772 0.7462372293205536 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1241 0.5226187367871371 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1128 0.47503137396929146 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 687 0.28931432084831843 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 623 0.2623621861550253 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 563 0.237094559880063 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 533 0.22446074674258185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 434 0.18276916338889404 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 424 0.178557892343067 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 416 0.17518887550640536 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 393 0.16550295210100313 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 374 0.15750153711393172 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 374 0.15750153711393172 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 373 0.15708041000934903 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 346 0.14570997818561598 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 346 0.14570997818561598 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 321 0.13518180057104837 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 316 0.13307616504813483 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 288 0.12128460611981909 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 275 0.11580995376024392 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 261 0.10991417429608605 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 259 0.10907192008692064 No Hit CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT 256 0.10780853877317251 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 249 0.10486064904109359 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 245 0.10317614062276277 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 244 0.10275501351818006 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.316906568740577E-4 0.0 20-21 0.0 0.0 0.0 0.003158453284370289 0.0 22-23 0.0 0.0 0.0 0.010738741166858981 0.0 24-25 0.0 0.0 0.0 0.04148101980139646 0.0 26-27 0.0 0.0 0.0 0.09264796300819514 0.0 28-29 0.0 0.0 0.0 0.20045650178136765 0.0 30-31 0.0 0.0 0.0 0.42154823168728783 0.0 32-33 0.0 0.0 0.0 0.6367441821290502 0.0 34-35 0.0 0.0 0.0 0.8784711401595229 0.0 36-37 0.0 0.0 0.0 1.177681947965535 0.0 38-39 0.0 0.0 0.0 1.5651188841816237 0.0 40-41 0.0 0.0 0.0 1.9700325952378948 0.0 42-43 0.0 0.0 0.0 2.3027230078582317 0.0 44-45 0.0 0.0 0.0 2.613514811040268 0.0 46-47 0.0 0.0 0.0 2.940520007748739 0.0 48-49 0.0 0.0 0.0 3.285212542849683 0.0 50-51 0.0 0.0 0.0 3.648434670552266 0.0 52-53 0.0 0.0 0.0 3.9705969055580357 0.0 54-55 0.0 0.0 0.0 4.250225303000952 0.0 56-57 0.0 0.0 0.0 4.536170607012608 0.0 58-59 0.0 0.0 0.0 4.8330652157434155 0.0 60-61 0.0 0.0 0.0 5.173546479798533 0.0 62-63 0.0 0.0 0.0 5.476968558650372 0.0 64-65 0.0 0.0 0.0 5.759965972929949 0.0 66-67 0.0 0.0 0.0 6.08549722477238 0.0 68-69 0.0 0.0 0.0 6.412712985033142 0.0 70-71 0.0 0.0 0.0 6.771302714585316 0.0 72-73 0.0 0.0 0.0 7.090095932754424 0.0 74-75 0.0 0.0 0.0 7.420470146299556 0.0 76-77 0.0 0.0 0.0 7.729156313958679 0.0 78-79 0.0 0.0 0.0 8.087746043510853 0.0 80-81 0.0 0.0 0.0 8.43770266741908 0.0 82-83 0.0 0.0 0.0 8.787027600670434 0.0 84-85 0.0 0.0 0.0 9.139932114310742 0.0 86-87 0.0 0.0 0.0 9.477676052186071 0.0 88-89 0.0 0.0 0.0 9.821736896630142 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGACG 15 6.5924576E-4 95.06857 3 GTATCGA 20 0.0020402977 71.52778 1 TATCGAC 20 0.0020661184 71.30142 2 TTAGTAA 25 0.0050020684 57.041138 2 GAGGCAT 25 0.0050020684 57.041138 6 GTGTAGC 50 2.7560114E-5 47.68518 1 GGTATCA 3640 0.0 45.196125 1 CCTGGCT 85 3.5920675E-7 39.162403 8 GTATCAA 6080 0.0 38.979504 1 GTGCATG 50 0.0015826733 38.148148 1 CGTCACT 75 6.717195E-6 38.148148 1 GTAGCCC 80 1.0687081E-5 35.65071 3 GAATGCA 110 6.902883E-8 34.58498 9 AGCCCTG 85 1.6187512E-5 33.55361 5 TGGGGAT 160 5.2750693E-11 32.679817 6 GTATATG 60 0.0038702292 31.790123 1 ATGGGCG 60 0.0039307745 31.689524 5 CATGGAT 75 3.0581653E-4 31.68952 6 TATCAAC 7595 0.0 30.98021 2 ATGGGGA 390 0.0 30.470695 5 >>END_MODULE