SRR938273_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR938273_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237458
Sequences flagged as poor quality	0
Sequence length	101
%GC	32

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11328	4.770527840712884	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9906	4.171685097996277	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8359	3.520201467206832	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6882	2.898196733738177	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2721	1.1458868515695406	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2575	1.0844022943004656	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT	2415	1.0170219575672328	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1918	0.8077217865896285	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1629	0.6860160533652268	No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT	1418	0.5971582342982759	RNA PCR Primer, Index 23 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1243	0.5234609909963025	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1128	0.47503137396929146	No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT	757	0.31879321816910783	RNA PCR Primer, Index 23 (96% over 25bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	739	0.3112129302866191	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	610	0.25688753379545015	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	552	0.23246216172965323	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG	521	0.2194072214875894	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	490	0.20635228124552554	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	438	0.18445367180722486	No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT	421	0.17729451102931887	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	418	0.17603112971557075	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	412	0.1735043670880745	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA	403	0.16971422314683018	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	368	0.1549747744864355	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC	363	0.15286913896352197	No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG	362	0.15244801185893925	No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG	359	0.15118463054519116	RNA PCR Primer, Index 23 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	359	0.15118463054519116	No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT	350	0.1473944866039468	RNA PCR Primer, Index 23 (96% over 28bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.14444659687186787	No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC	318	0.13391841925730025	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	312	0.131391656629804	No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT	301	0.12675925847939426	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA	299	0.12591700427022884	No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT	273	0.1149676995510785	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	271	0.1141254453419131	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	268	0.11286206402816498	No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG	264	0.11117755560983417	Illumina PCR Primer Index 1 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCACT	50	5.520633E-9	66.53776	1
CGCAGAA	30	1.2995227E-4	63.32926	9
TAACTGA	30	1.2995227E-4	63.32926	4
GGTATCA	3840	0.0	54.08668	1
ACGCCCC	20	5.386673E-4	47.49695	82-83
TCAGAGT	40	5.381199E-4	47.49695	4
GAGCACT	45	9.6126704E-4	42.21951	4
GATAGCA	45	9.6126704E-4	42.21951	9
ACTGGGC	45	9.6126704E-4	42.21951	8
ATATGGG	50	0.0016137442	37.99756	3
GTATCAA	6340	0.0	37.48184	1
GTCACTA	95	8.622919E-7	35.01987	1
GGGATGT	95	8.6656837E-7	34.99775	7
TTTCAGA	55	0.00256836	34.56507	1
ATGGGTG	60	0.0039459383	31.66463	5
CATGGAT	60	0.0039459383	31.66463	4
GGATGTA	60	0.0039459383	31.66463	8
ATGGGGG	500	0.0	30.398046	5
TATCAAC	7985	0.0	29.760157	2
AGTACAA	80	4.4847146E-4	29.685593	7

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content