##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938272_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 235299 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.404005116893824 33.0 31.0 34.0 30.0 34.0 2 31.371251046540785 33.0 31.0 34.0 30.0 34.0 3 31.234854376771683 33.0 31.0 34.0 28.0 34.0 4 34.84006731860314 37.0 35.0 37.0 33.0 37.0 5 34.89945133638477 37.0 35.0 37.0 33.0 37.0 6 35.07124552165543 37.0 35.0 37.0 33.0 37.0 7 35.0175861350877 37.0 35.0 37.0 33.0 37.0 8 35.02536347370792 37.0 35.0 37.0 33.0 37.0 9 36.51589254522969 39.0 37.0 39.0 33.0 39.0 10-11 36.48156388254944 39.0 37.0 39.0 32.5 39.0 12-13 36.30135912179822 39.0 37.0 39.0 32.0 39.0 14-15 37.0954976434239 40.0 37.5 41.0 32.0 41.0 16-17 37.03420966514945 40.0 37.5 41.0 32.0 41.0 18-19 37.32207319198127 40.0 37.0 41.0 33.0 41.0 20-21 37.43647231819939 40.0 37.5 41.0 33.0 41.0 22-23 37.22782927254259 40.0 36.5 41.0 33.0 41.0 24-25 37.001166600793034 40.0 36.0 41.0 32.5 41.0 26-27 36.69450783896234 40.0 35.0 41.0 32.0 41.0 28-29 36.33444468527278 39.5 35.0 41.0 31.5 41.0 30-31 35.589581766178355 38.0 35.0 40.5 31.0 41.0 32-33 34.71196860165152 36.0 35.0 40.0 30.0 41.0 34-35 34.21031538595574 35.0 35.0 40.0 27.5 41.0 36-37 33.736913459045724 35.0 35.0 40.0 23.5 41.0 38-39 33.18149885889868 35.0 35.0 39.5 19.5 41.0 40-41 32.408040408161526 35.0 33.5 39.0 6.0 41.0 42-43 31.60224437842915 35.0 33.0 39.0 2.0 41.0 44-45 30.511464137119155 35.0 32.0 39.0 2.0 41.0 46-47 29.221728524133127 35.0 28.0 39.0 2.0 41.0 48-49 28.10837275126541 35.0 22.0 38.0 2.0 41.0 50-51 27.10483682463589 34.0 19.0 37.5 2.0 40.0 52-53 26.999366763139662 35.0 12.0 38.0 2.0 40.0 54-55 26.864999426261903 35.0 2.0 38.0 2.0 40.0 56-57 26.478552820028987 35.0 2.0 37.0 2.0 40.0 58-59 26.068306707635816 35.0 2.0 36.5 2.0 40.0 60-61 25.629645684852036 35.0 2.0 36.0 2.0 40.0 62-63 25.136581540933026 34.5 2.0 35.0 2.0 40.0 64-65 24.675795477243845 34.0 2.0 35.0 2.0 39.5 66-67 24.305375288462763 33.5 2.0 35.0 2.0 39.0 68-69 23.79746195266448 33.0 2.0 35.0 2.0 39.0 70-71 23.381722829251295 33.0 2.0 35.0 2.0 38.0 72-73 22.861452874852848 33.0 2.0 35.0 2.0 37.0 74-75 22.356588850781346 32.5 2.0 35.0 2.0 37.0 76-77 21.861956489402843 31.5 2.0 35.0 2.0 36.0 78-79 21.314780343307877 31.0 2.0 35.0 2.0 35.5 80-81 20.763558281165665 30.0 2.0 35.0 2.0 35.0 82-83 20.207752689131702 29.0 2.0 35.0 2.0 35.0 84-85 19.641768558302417 28.0 2.0 35.0 2.0 35.0 86-87 19.052643657644104 26.5 2.0 34.5 2.0 35.0 88-89 18.401950284531594 24.5 2.0 34.0 2.0 35.0 90-91 17.73547911380839 21.5 2.0 34.0 2.0 35.0 92-93 17.061668345381833 14.0 2.0 33.5 2.0 35.0 94-95 16.35067934840352 2.0 2.0 33.0 2.0 35.0 96-97 15.506901856786472 2.0 2.0 33.0 2.0 35.0 98-99 14.587040318913383 2.0 2.0 33.0 2.0 35.0 100-101 11.971228097016986 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.38725427745644936 1101 2 -0.31536106757313576 1101 3 -0.37865590464807397 1101 4 -0.5368445337365344 1101 5 -0.35271284696251115 1101 6 -0.2544011426048982 1101 7 -0.9605164891721785 1101 8 -0.3636781373256497 1101 9 -0.5385170690793757 1101 10-11 -0.36696622777692056 1101 12-13 -0.4808264975536076 1101 14-15 -0.3872853989175411 1101 16-17 -0.557935130401269 1101 18-19 -0.910593941877174 1101 20-21 -0.8801183878040248 1101 22-23 -1.2358713460804225 1101 24-25 -0.8925030437902208 1101 26-27 -0.9092058038774056 1101 28-29 -0.7460704408429635 1101 30-31 -0.42354942553227914 1101 32-33 -0.7860278604313322 1101 34-35 -0.5160885843405367 1101 36-37 -1.0619673523428048 1101 38-39 -0.897677418724399 1101 40-41 -0.5824801832629589 1101 42-43 -0.5594922650999337 1101 44-45 -0.7843477590794947 1101 46-47 -0.7051371932996204 1101 48-49 -0.1447242489334677 1101 50-51 -0.2291310068667336 1101 52-53 -0.009032904406929276 1101 54-55 -0.6503495748042205 1101 56-57 -0.3046174107940729 1101 58-59 -0.1381969527352176 1101 60-61 -0.02048198945789892 1101 62-63 -0.1745094780233707 1101 64-65 -0.10419146818506064 1101 66-67 -0.4055099670767568 1101 68-69 -0.14887281593102486 1101 70-71 -0.2988051159977374 1101 72-73 0.007788268138003929 1101 74-75 0.012660691729333706 1101 76-77 -0.020938272781240386 1101 78-79 -0.2859710487210201 1101 80-81 -0.4332948190510564 1101 82-83 -0.7504617238861826 1101 84-85 -0.5452778115229897 1101 86-87 0.009200891405516387 1101 88-89 -0.2602227144538496 1101 90-91 -0.019943334039254523 1101 92-93 0.2980427001566035 1101 94-95 0.23697238249290464 1101 96-97 0.24047127468707785 1101 98-99 -0.17076510219267682 1101 100-101 0.33338010605729096 1102 1 0.5714299330698651 1102 2 0.5717441955847598 1102 3 0.19450199008876723 1102 4 0.30420809784241243 1102 5 0.6925503109322264 1102 6 0.5408620152898393 1102 7 0.3579045634593996 1102 8 0.7642165995164589 1102 9 0.7914829309206226 1102 10-11 0.5965864038020285 1102 12-13 0.9296998182358678 1102 14-15 1.0508724958192985 1102 16-17 0.7064069748618849 1102 18-19 1.0162481633859883 1102 20-21 0.6326447700907138 1102 22-23 0.7553128644458909 1102 24-25 0.2872337983150359 1102 26-27 0.30342577506996804 1102 28-29 0.47656113810440104 1102 30-31 0.8489505744677146 1102 32-33 0.6125247711476121 1102 34-35 0.561016678817353 1102 36-37 0.27263791081509225 1102 38-39 0.5607436339071867 1102 40-41 0.4862040272633621 1102 42-43 0.45813931384743256 1102 44-45 0.9493364514468254 1102 46-47 0.802099648805644 1102 48-49 0.3411968036981108 1102 50-51 -0.576104691077262 1102 52-53 -0.8965329044069321 1102 54-55 -0.5327179958568529 1102 56-57 -0.7243542528993316 1102 58-59 -0.9975390579983774 1102 60-61 -0.8044293578789485 1102 62-63 -0.8384568464444229 1102 64-65 -0.901823047132428 1102 66-67 -0.7987994407609627 1102 68-69 -0.799793868562606 1102 70-71 -0.5599893265240539 1102 72-73 -0.6045801529146289 1102 74-75 -0.22089193984961142 1102 76-77 -0.29475406225492407 1102 78-79 -0.7834710487210188 1102 80-81 -0.6040842927352692 1102 82-83 -0.29638277651776335 1102 84-85 -0.07159360099667467 1102 86-87 -0.23014121385763886 1102 88-89 0.09477728554615084 1102 90-91 -0.4116538603550417 1102 92-93 -0.4809046682644471 1102 94-95 0.10723554038764149 1102 96-97 0.29718180100286595 1102 98-99 0.498445424123112 1102 100-101 0.541406421846764 1103 1 0.4824695370302621 1103 2 0.5227837995451559 1103 3 0.413214861375895 1103 4 0.6891585928919213 1103 5 0.1426493208332147 1103 6 0.6835352826165675 1103 7 0.48315208821187383 1103 8 0.36550372822932786 1103 9 0.620888871514687 1103 10-11 0.7100269978614264 1103 12-13 0.8064324915031946 1103 14-15 0.8813180403737562 1103 16-17 0.4888079649608983 1103 18-19 0.6341689554651921 1103 20-21 0.37464972058575796 1103 22-23 0.6467237555349996 1103 24-25 0.5690654814833565 1103 26-27 0.6053069631887809 1103 28-29 0.688739355926181 1103 30-31 0.6487278021904856 1103 32-33 0.6681930879792972 1103 34-35 1.20423450059954 1103 36-37 0.9014250395279575 1103 38-39 1.0454961091547048 1103 40-41 1.0110060074613827 1103 42-43 1.5357135712731775 1103 44-45 1.8150790257042502 1103 46-47 2.000985787419502 1103 48-49 1.5212215561733586 1103 50-51 2.0115933287247216 1103 52-53 2.3018581847019775 1103 54-55 2.2329750734500777 1103 56-57 2.096685351061062 1103 58-59 2.2264460905164754 1103 60-61 1.884729057962634 1103 62-63 1.840602559496169 1103 64-65 1.6560482399962844 1103 66-67 1.8187995691400225 1103 68-69 1.9242902898532357 1103 70-71 2.208203742782878 1103 72-73 1.8346772728279461 1103 74-75 2.1797268720315763 1103 76-77 1.7997756407153744 1103 78-79 1.474920040387893 1103 80-81 1.3736879844924559 1103 82-83 1.69398851061095 1103 84-85 1.6521687752409484 1103 86-87 1.5183983901027567 1103 88-89 1.3170050083184286 1103 90-91 1.3968362386548598 1103 92-93 1.5601844406464416 1103 94-95 1.4268890057341785 1103 96-97 1.3236917019929635 1103 98-99 0.8609701765983591 1103 100-101 0.4894509763022086 1104 1 0.1892336713876226 1104 2 0.2575853170800855 1104 3 -0.06596529963086084 1104 4 0.31504921933774455 1104 5 0.4095596567266213 1104 6 0.45086201528983594 1104 7 0.34435316159024154 1104 8 0.49888949671272087 1104 9 0.3138193795187547 1104 10-11 0.45829201127865815 1104 12-13 0.3909147715068926 1104 14-15 -0.04609012100313237 1104 16-17 0.310682675796464 1104 18-19 0.6739117147878559 1104 20-21 0.6101914990626724 1104 22-23 0.586878285006641 1104 24-25 0.6938225833617722 1104 26-27 0.6929584853503314 1104 28-29 0.71029945586141 1104 30-31 0.32738515390696676 1104 32-33 0.32848271507284466 1104 34-35 0.19657742648090704 1104 36-37 0.5932220229646248 1104 38-39 0.35719223203802386 1104 40-41 1.103353559973641 1104 42-43 1.2347280988941662 1104 44-45 0.8402710308860755 1104 46-47 0.7727772188991011 1104 48-49 0.3321080186513825 1104 50-51 -0.16953927051651263 1104 52-53 -0.3543600072106692 1104 54-55 -0.3939796780998428 1104 56-57 -0.3484196734600822 1104 58-59 -0.02690821687687972 1104 60-61 -0.32559758217801615 1104 62-63 -0.08990544457526539 1104 64-65 -0.6058884676931768 1104 66-67 -0.45167327253666656 1104 68-69 -0.349536859216812 1104 70-71 -0.6664145601689171 1104 72-73 0.02210209007602515 1104 74-75 -0.17661623891503453 1104 76-77 -0.6023942491708141 1104 78-79 -0.6228869365714864 1104 80-81 -0.5126356946044233 1104 82-83 -0.5001911877327174 1104 84-85 -0.4131823860434025 1104 86-87 -0.2982019615211904 1104 88-89 -0.004615237818335061 1104 90-91 -0.4363968510092491 1104 92-93 -0.9196897149934209 1104 94-95 -1.091409319425443 1104 96-97 -1.6024210027354506 1104 98-99 -1.0547788749423095 1104 100-101 -1.7902057276859473 1105 1 0.10697787827534455 1105 2 0.2934108622514273 1105 3 0.22742436451799009 1105 4 0.34859165948625304 1105 5 -0.07576019135088075 1105 6 -0.053704194755823664 1105 7 0.10836118446396625 1105 8 -0.0012171904378845966 1105 9 0.14595781676537456 1105 10-11 0.16824165494357857 1105 12-13 -0.2155056612161914 1105 14-15 -0.5421640338610629 1105 16-17 0.09077912098060636 1105 18-19 -0.17763311515282254 1105 20-21 -0.2901520335622578 1105 22-23 0.08005030736826058 1105 24-25 -0.07995798250687614 1105 26-27 -0.34584363132273666 1105 28-29 -0.270379501165003 1105 30-31 -0.35185992781539 1105 32-33 -0.1439706626423387 1105 34-35 -0.013937659082188247 1105 36-37 0.16826576469636478 1105 38-39 0.22051532340490354 1105 40-41 0.2606104199574233 1105 42-43 0.003184975947888802 1105 44-45 -0.41431651658970736 1105 46-47 -0.31702135576056634 1105 48-49 0.03168767127801786 1105 50-51 -0.5633535495247486 1105 52-53 -0.6683936350005375 1105 54-55 -0.5344759867244342 1105 56-57 -0.4283496866892875 1105 58-59 -0.17526736850065916 1105 60-61 0.0882418749977596 1105 62-63 0.15065274259667305 1105 64-65 0.46030024053880325 1105 66-67 0.592399189376021 1105 68-69 0.5781399213917311 1105 70-71 0.1279672944805128 1105 72-73 0.22959792927715483 1105 74-75 -0.12711340103682645 1105 76-77 -0.06803031796268755 1105 78-79 -0.050126299862572665 1105 80-81 -0.1321893155663183 1105 82-83 -0.15701063039904284 1105 84-85 -0.39510958273183405 1105 86-87 -0.4838055974192841 1105 88-89 -0.6337386961890097 1105 90-91 0.006120112247696596 1105 92-93 0.1200085737446841 1105 94-95 0.5509113394743999 1105 96-97 0.15504709780651993 1105 98-99 0.6260025017486832 1105 100-101 -0.09307987952309915 1106 1 -0.0768353730525817 1106 2 -0.1742762125785049 1106 3 -0.20019188746225325 1106 4 -0.2851796572596257 1106 5 -0.11275581151674885 1106 6 -0.17158696430200138 1106 7 0.02831272672470675 1106 8 -0.20476299232027628 1106 9 2.5844112470707614E-4 1106 10-11 -0.31836257578981275 1106 12-13 -0.3162185491110776 1106 14-15 -0.07412750418070146 1106 16-17 -0.1971134333013822 1106 18-19 0.5742073470594491 1106 20-21 0.5343284435600992 1106 22-23 0.19087408893568636 1106 24-25 0.1501929819885106 1106 26-27 0.3138339383352644 1106 28-29 -0.004153147609883945 1106 30-31 -0.16946779287922453 1106 32-33 0.08961660788230574 1106 34-35 -0.4229629130193757 1106 36-37 0.03013791081509254 1106 38-39 -0.5532359579295516 1106 40-41 -0.18736740130807306 1106 42-43 -0.8803300739076683 1106 44-45 -1.3326023240633802 1106 46-47 -0.9343799430310895 1106 48-49 -0.7722215636488272 1106 50-51 -0.23901285434256891 1106 52-53 -0.20852270032529674 1106 54-55 -0.08292207748950275 1106 56-57 0.02717635934556384 1106 58-59 -0.39289620085551746 1106 60-61 -0.23514364359323636 1106 62-63 -0.38610990766891007 1106 64-65 -0.003251618560998537 1106 66-67 -0.27078923667933097 1106 68-69 -0.5014775420319957 1106 70-71 -0.941877081626096 1106 72-73 -1.0686617855676879 1106 74-75 -0.5721674500536942 1106 76-77 -0.7370499806222739 1106 78-79 -0.8033179874965306 1106 80-81 -0.6450026600822021 1106 82-83 -0.6923521642728652 1106 84-85 -0.30322625405789694 1106 86-87 -0.6633554995719244 1106 88-89 -1.0750186328211981 1106 90-91 -0.9888477379060632 1106 92-93 -0.5962107907134282 1106 94-95 -0.8909277249184804 1106 96-97 -1.3646039132828491 1106 98-99 -1.3865545758768878 1106 100-101 -0.5224201087654805 1107 1 -0.07486038951077845 1107 2 -0.09502999796362843 1107 3 -0.19130446152413683 1107 4 -0.22304996667371313 1107 5 -0.1458367858419649 1107 6 -0.06526701696822812 1107 7 0.21354972474971845 1107 8 -0.005783400483544199 1107 9 -0.3981944884342141 1107 10-11 -0.0870426284560395 1107 12-13 -0.12513889144155144 1107 14-15 -0.17695008482586871 1107 16-17 -0.15012528320262675 1107 18-19 0.0996352601601771 1107 20-21 -0.05590361700605939 1107 22-23 0.07007092896201783 1107 24-25 0.3510241208956799 1107 26-27 0.10189351700545046 1107 28-29 0.14446436391084916 1107 30-31 0.251450574467718 1107 32-33 0.2209118679218065 1107 34-35 -0.08083815989232335 1107 36-37 -0.1976846698300676 1107 38-39 -0.015546688673460096 1107 40-41 0.27346209177948566 1107 42-43 0.2911231848151772 1107 44-45 0.22804612886617548 1107 46-47 0.06701900364435431 1107 48-49 0.26563228756907975 1107 50-51 0.06365337343886779 1107 52-53 -0.03645225924564244 1107 54-55 -0.41747606037298013 1107 56-57 -0.37798328515739854 1107 58-59 -0.5081035741274071 1107 60-61 -0.5280583901370157 1107 62-63 -0.5599084593476462 1107 64-65 -0.3169036922937174 1107 66-67 -0.4717026665674169 1107 68-69 -0.39108419114325343 1107 70-71 0.029526802508200944 1107 72-73 0.3128392019240849 1107 74-75 0.21539838273103484 1107 76-77 0.22250400226120348 1107 78-79 0.2636257254725294 1107 80-81 -0.2986810669288147 1107 82-83 -0.15194729264679552 1107 84-85 -0.4736097300289295 1107 86-87 -0.9945766977286077 1107 88-89 -1.1477227144538489 1107 90-91 -0.9537506345485909 1107 92-93 -0.5506627327805766 1107 94-95 -0.7791354273542943 1107 96-97 -1.0132214248035858 1107 98-99 -0.7292965113607597 1107 100-101 -0.24641615879839662 1108 1 -0.06679587338174642 1108 2 -0.03051386893136865 1108 3 0.27240521589521904 1108 4 0.2164661623585431 1108 5 0.48319547222254755 1108 6 0.4065071765801562 1108 7 0.342581982814238 1108 8 0.3813133737100074 1108 9 -0.09577513359550238 1108 10-11 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sequence quality scores pass #Quality Count 2 5115.0 3 738.0 4 578.0 5 485.0 6 472.0 7 583.0 8 692.0 9 773.0 10 1049.0 11 1589.0 12 2509.0 13 3498.0 14 4354.0 15 6381.0 16 8046.0 17 10842.0 18 9188.0 19 4807.0 20 4212.0 21 3899.0 22 3834.0 23 3972.0 24 4005.0 25 4209.0 26 4371.0 27 4777.0 28 5363.0 29 6042.0 30 6856.0 31 8022.0 32 9566.0 33 11723.0 34 17747.0 35 32013.0 36 17703.0 37 15527.0 38 9146.0 39 613.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.41000323453806 21.227507192591204 15.616860455218672 11.745629117652067 2 17.46201829244958 26.431781749220807 38.54000569778764 17.56619426054197 3 19.34172494966307 36.118288587032865 31.787822933206193 12.752163530097866 4 10.78535680239684 23.586664169957782 34.78780811657361 30.84017091107177 5 8.890601777524157 43.637494091243965 29.126440991223102 18.34546314000877 6 21.096904441453567 41.16990646881442 17.171235327188782 20.56195376254323 7 19.785613636266884 46.567306505238335 18.180270154063592 15.466809704431192 8 27.58229140086757 32.10895636642 17.53253380964532 22.77621842306711 9 30.976422380229014 17.80682606346086 19.43909008514495 31.77766147116518 10-11 23.836030774008428 33.24286325049932 20.755864779840767 22.16524119565148 12-13 29.35912989804823 34.00102164612732 24.01975182512824 12.62009663069621 14-15 24.665104708643756 31.52856957416428 30.810366804774397 12.995958912417565 16-17 14.636642784032752 35.94439254623492 30.084458129493953 19.334506540238372 18-19 13.74901848963376 29.076539721112738 42.668516552148255 14.505925237105242 20-21 17.970094881828608 26.617342585322362 43.55137641423717 11.86118611861186 22-23 16.643171693597157 28.926286828902647 42.93851841125714 11.492023066243046 24-25 14.43495929764476 29.859469314556154 44.03990577330215 11.66566561449694 26-27 13.556786161219748 30.504737500744888 44.250512910008766 11.687963428026595 28-29 13.156948478175302 31.4082064702816 43.74897605293673 11.685868998606368 30-31 13.345159396615532 31.709163922849825 43.36209023541523 11.583586445119408 32-33 12.173985199811062 32.30317833844688 43.40566049779359 12.117175963948476 34-35 12.308552882048795 32.47066480350683 43.284861400900525 11.935920913543853 36-37 12.67612223760578 32.33269317884555 42.40949044978828 12.581694133760388 38-39 12.48761571072001 32.95305238182305 42.154207256662005 12.405124650794933 40-41 12.20253788974128 33.1133375291296 41.84052288693463 12.843601694194492 42-43 12.147171953638795 33.138992190207674 41.262196820374776 13.451639035778756 44-45 12.656085915810328 32.896129792055504 40.58531421258721 13.862470079546952 46-47 12.7641165078265 33.53883700511704 39.30193945861829 14.395107028438165 48-49 12.899982356454881 33.551220486919334 37.76122543986634 15.787571716759455 50-51 13.3457355770764 33.91871923800615 36.2754998990103 16.460045285907153 52-53 13.586201033009543 34.23788976980467 35.19225455395668 16.983654643229112 54-55 13.707047212453915 33.44013505066526 34.902262608910114 17.95055512797071 56-57 14.37449785966001 33.46220716450649 34.052235348129386 18.11105962770412 58-59 14.730495126970897 32.74837140475907 34.3818566836188 18.139276784651233 60-61 15.324963543371698 32.9285194995132 34.44332960618338 17.30318735093172 62-63 15.873130615594611 32.711941523402125 34.431328692738475 16.983599168264796 64-65 16.178893416921238 33.02274857940957 34.38073311607282 16.41762488759638 66-67 16.366148678812102 32.469547841244875 34.358750876894625 16.805552603048405 68-69 16.332246005452234 31.971267325301 34.769896186413646 16.92659048283312 70-71 16.228507523297026 32.114496140018154 34.69391226328244 16.963084073402378 72-73 15.982030288276528 31.332399271176385 35.022866096732834 17.662704343814248 74-75 14.968005272220921 31.956036480367356 35.17017793745615 17.905780309955567 76-77 14.483010735319436 31.434231025094423 35.64121801561308 18.441540223973067 78-79 14.278700336457021 31.191669978809372 35.76689033371521 18.762739351018404 80-81 14.516934652539401 30.73828071033083 35.414944153373646 19.329840483756126 82-83 14.71734685636315 30.60747365602139 34.64737962959027 20.02779985802519 84-85 15.441820330366326 30.019043246873593 34.296972633834066 20.242163788926014 86-87 15.438030441186784 29.913641571073008 34.111260603510026 20.53706738423018 88-89 16.066283493130733 29.463535785899133 33.892571068909575 20.57760965206057 90-91 16.552886139340547 29.227944696068775 33.783775159182824 20.435394005407854 92-93 17.07865885430513 28.90800625272493 33.97739235848956 20.035942534480373 94-95 17.362345715768253 28.429341862383993 33.992080572308375 20.21623184953938 96-97 17.693714382920813 28.127711150803776 33.80411669643617 20.374457769839243 98-99 18.104773467083678 27.999761868819483 33.391732170459406 20.503732493637433 100-101 19.603324566555166 26.831235264577963 33.65506558060755 19.910374588259327 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7448.0 1 4644.0 2 2150.0 3 2284.0 4 1680.5 5 1492.0 6 2668.5 7 5297.0 8 7566.0 9 7567.5 10 5134.0 11 2899.5 12 2473.0 13 2384.0 14 2205.5 15 1910.5 16 1659.5 17 1569.0 18 1538.0 19 1465.0 20 1466.5 21 1525.0 22 1582.5 23 1692.5 24 1840.5 25 2158.5 26 2615.0 27 3042.5 28 3276.5 29 3494.5 30 3999.0 31 4490.0 32 4846.5 33 5325.5 34 6005.5 35 6392.5 36 6274.0 37 6276.5 38 5913.0 39 5225.5 40 4871.0 41 4704.0 42 4679.0 43 4608.5 44 4580.5 45 4480.0 46 4385.5 47 4530.0 48 5059.5 49 5289.0 50 4861.0 51 4296.5 52 3775.5 53 3340.5 54 2987.5 55 2936.5 56 3062.0 57 3072.5 58 2832.0 59 2764.5 60 3009.0 61 3220.0 62 2738.0 63 1507.5 64 672.0 65 422.0 66 351.0 67 339.0 68 322.5 69 295.0 70 262.5 71 236.0 72 200.5 73 206.5 74 191.0 75 121.0 76 90.0 77 76.5 78 59.5 79 39.0 80 25.0 81 18.5 82 9.5 83 5.5 84 3.5 85 2.5 86 2.5 87 2.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14237204578005008 2 0.05099894177195823 3 0.16234663130740037 4 0.13727215160285422 5 0.20314578472496697 6 0.2171704937122555 7 0.16829650784746217 8 0.06757359784784465 9 0.12239746025269975 10-11 0.09796046732030311 12-13 0.16319661367026633 14-15 0.14343452373363252 16-17 0.14045958546360163 18-19 0.14003459428216866 20-21 0.13705965601213774 22-23 0.21122061717219368 24-25 0.23204518506240995 26-27 0.1551217812230396 28-29 0.12813484120204505 30-31 0.09562301582242168 32-33 0.12834733679276156 34-35 0.09116060841737533 36-37 0.08478574069588056 38-39 0.0518489241348242 40-41 0.06077373894491689 42-43 0.06183621689849936 44-45 0.039099188691834645 46-47 0.044411578459746956 48-49 0.03633674601252024 50-51 0.05376138445127264 52-53 0.027199435611711058 54-55 0.054398871223422116 56-57 0.023799506160247175 58-59 0.05397388004198913 60-61 0.03739922396610271 62-63 0.054398871223422116 64-65 0.04143664018971606 66-67 0.03994917105470061 68-69 0.07054853611787555 70-71 0.05503635799557159 72-73 0.05333639326983965 74-75 0.044199082869030466 76-77 0.020187081118066802 78-79 0.022737028206664713 80-81 0.024012001750963668 82-83 0.019549594345917323 84-85 0.01912460316448434 86-87 0.024861984113829637 88-89 0.06693611107569518 90-91 0.11496011457762252 92-93 0.08563572305874653 94-95 0.07692340383937032 96-97 0.06757359784784465 98-99 0.057161313902736516 100-101 0.1364221692399883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 235299.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.13915061262479 #Duplication Level Percentage of deduplicated Percentage of total 1 80.78625961897271 34.04264361514498 2 9.893800490151584 8.338326979715172 3 3.507710306294313 4.434357987071768 4 1.7256159672425442 2.908639645727351 5 0.930884592498462 1.9613343023132268 6 0.5960485310580619 1.5070187293613657 7 0.3882888061884159 1.1453512339618954 8 0.2975199943521628 1.0029791881818453 9 0.22591348723689653 0.8567822217688983 >10 1.3887628210946719 11.203192533754924 >50 0.1452300989380049 4.390583895384171 >100 0.09984569301987836 7.222300137272152 >500 0.004034169414944581 1.037403473877917 >1k 0.006051254122416872 4.679152907577168 >5k 0.003025627061208436 10.444158283715613 >10k+ 0.0010085423537361453 4.825774865171548 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11355 4.825774865171548 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9410 3.999167017284391 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8495 3.6103000862732095 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6670 2.834691180158012 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2844 1.20867491999541 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2276 0.9672799289414745 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1794 0.7624341794907755 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1764 0.749684444047786 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1200 0.5099894177195823 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1132 0.4810900173821393 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 718 0.3051436682688834 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 604 0.2566946735855231 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 575 0.24436992932396653 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 544 0.23119520269954397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 447 0.1899710581005444 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 422 0.1793462785647198 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.17637134029468887 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 407 0.172971410843225 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 397 0.16872149902889516 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 367 0.1559717635859056 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 359 0.1525718341344417 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 340 0.144497001687215 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 338 0.14364701932434903 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 338 0.14364701932434903 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 327 0.1389721163285862 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 320 0.13599717805855527 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 274 0.11644758371263796 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 268 0.11389763662404005 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 265 0.1126226630797411 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 264 0.11219767189830812 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.499823628659706E-4 0.0 18-19 0.0 0.0 0.0 0.0012749735442989557 0.0 20-21 0.0 0.0 0.0 0.0025499470885979115 0.0 22-23 0.0 0.0 0.0 0.010412283945108139 0.0 24-25 0.0 0.0 0.0 0.028686904746726506 0.0 26-27 0.0 0.0 0.0 0.06842358021071063 0.0 28-29 0.0 0.0 0.0 0.15193434736229222 0.0 30-31 0.0 0.0 0.0 0.3489177599564809 0.0 32-33 0.0 0.0 0.0 0.5469636505042521 0.0 34-35 0.0 0.0 0.0 0.7900586062839197 0.0 36-37 0.0 0.0 0.0 1.10688953204221 0.0 38-39 0.0 0.0 0.0 1.5044687822727678 0.0 40-41 0.0 0.0 0.0 1.9113978384948513 0.0 42-43 0.0 0.0 0.0 2.2348161275653533 0.0 44-45 0.0 0.0 0.0 2.557171938682272 0.0 46-47 0.0 0.0 0.0 2.895252423512212 0.0 48-49 0.0 0.0 0.0 3.251820024734487 0.0 50-51 0.0 0.0 0.0 3.605200192096014 0.0 52-53 0.0 0.0 0.0 3.9041814882341193 0.0 54-55 0.0 0.0 0.0 4.1715009413554665 0.0 56-57 0.0 0.0 0.0 4.470694733084288 0.0 58-59 0.0 0.0 0.0 4.76691358654308 0.0 60-61 0.0 0.0 0.0 5.1039315934194365 0.0 62-63 0.0 0.0 0.0 5.403337880738975 0.0 64-65 0.0 0.0 0.0 5.704019141602812 0.0 66-67 0.0 0.0 0.0 6.010437783415994 0.0 68-69 0.0 0.0 0.0 6.3480932770645015 0.0 70-71 0.0 0.0 0.0 6.708910790101106 0.0 72-73 0.0 0.0 0.0 7.072065754635592 0.0 74-75 0.0 0.0 0.0 7.409508752693382 0.0 76-77 0.0 0.0 0.0 7.709127535603637 0.0 78-79 0.0 0.0 0.0 8.051457932247907 0.0 80-81 0.0 0.0 0.0 8.417587835052423 0.0 82-83 0.0 0.0 0.0 8.775430409818995 0.0 84-85 0.0 0.0 0.0 9.129660559543389 0.0 86-87 0.0 0.0 0.0 9.464341114921865 0.0 88-89 0.0 0.0 0.0 9.823883654414171 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 55 1.4915713E-10 69.23754 1 ACTACAC 25 0.0049870024 57.08444 3 TGGGCGA 25 0.0049870024 57.08444 6 GGTATCA 3695 0.0 47.5364 1 ATGGGCG 55 4.9081224E-5 43.245785 5 GTCACTA 110 1.5752448E-9 38.946117 1 GTATCAA 6095 0.0 38.580475 1 GTCGATG 25 0.0016132431 38.007668 30-31 GGTGCCT 70 2.0280924E-4 33.986076 9 TCACTAC 140 4.456524E-10 33.971584 2 ATGGGTA 135 1.1716111E-8 31.713575 5 ACATGAG 90 2.3806324E-5 31.713575 3 CTATAGC 60 0.0039161183 31.713573 4 CACTACC 165 7.4578566E-11 31.713573 3 TACATGA 105 1.8745232E-6 31.706814 2 GACTATT 30 0.0039405855 31.6798 94-95 CGAAGAG 30 0.0039488114 31.666313 90-91 GCTAGCT 30 0.0039653033 31.639368 46-47 TATCAAC 7550 0.0 30.929893 2 ATGGGGT 280 0.0 30.580944 5 >>END_MODULE