##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938272_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 235299 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31543695468319 33.0 31.0 34.0 31.0 34.0 2 32.466381072592746 34.0 31.0 34.0 31.0 34.0 3 32.39761324952507 34.0 31.0 34.0 30.0 34.0 4 35.99659582063672 37.0 35.0 37.0 35.0 37.0 5 36.03361680245135 37.0 35.0 37.0 35.0 37.0 6 36.15009413554669 37.0 36.0 37.0 35.0 37.0 7 36.09118610788826 37.0 35.0 37.0 35.0 37.0 8 36.129341816157314 37.0 36.0 37.0 35.0 37.0 9 37.68011763755902 39.0 37.0 39.0 35.0 39.0 10-11 37.70849642369921 39.0 37.5 39.0 35.0 39.0 12-13 37.61163668353882 39.0 37.0 39.0 35.0 39.0 14-15 38.474776773381954 40.0 38.0 41.0 34.0 41.0 16-17 38.42727550903319 40.0 37.5 41.0 34.5 41.0 18-19 38.77539216061267 40.0 38.0 41.0 35.0 41.0 20-21 38.86893696955788 40.0 38.0 41.0 35.0 41.0 22-23 38.67309040837402 40.0 38.0 41.0 35.0 41.0 24-25 38.45908397400754 40.0 37.0 41.0 35.0 41.0 26-27 38.09762472428697 40.0 36.5 41.0 34.0 41.0 28-29 37.742958958601605 39.5 35.0 41.0 33.5 41.0 30-31 36.96472785689697 38.5 35.0 40.5 33.0 41.0 32-33 36.11203830020527 36.5 35.0 40.0 33.0 41.0 34-35 35.517996251577785 35.0 35.0 40.0 31.5 41.0 36-37 35.0437443423049 35.0 35.0 40.0 31.0 41.0 38-39 34.53491940042244 35.0 35.0 40.0 29.0 41.0 40-41 34.003130060051255 35.0 35.0 40.0 25.0 41.0 42-43 33.23119732765545 35.0 34.5 40.0 18.5 41.0 44-45 32.19834550933068 35.0 33.0 40.0 2.0 41.0 46-47 30.856448603691472 35.0 32.0 39.5 2.0 41.0 48-49 29.752585008861068 35.0 30.0 39.0 2.0 41.0 50-51 29.015352806429267 35.0 27.0 39.0 2.0 41.0 52-53 28.436867559998127 35.0 24.5 38.0 2.0 40.5 54-55 28.04919272925087 35.0 23.0 38.0 2.0 40.0 56-57 27.531557720177304 35.0 21.0 37.0 2.0 40.0 58-59 26.9564022796527 35.0 15.5 37.0 2.0 40.0 60-61 26.370201318322643 35.0 4.5 36.0 2.0 40.0 62-63 25.818873008385076 34.0 2.0 35.5 2.0 40.0 64-65 25.23964615234234 33.5 2.0 35.0 2.0 39.0 66-67 24.59321119086779 33.0 2.0 35.0 2.0 39.0 68-69 24.03154072053005 33.0 2.0 35.0 2.0 38.5 70-71 23.62717861104382 33.0 2.0 35.0 2.0 38.0 72-73 23.203326405977077 33.0 2.0 35.0 2.0 37.0 74-75 22.758826004360408 32.5 2.0 35.0 2.0 36.5 76-77 21.62490915813497 30.0 2.0 33.5 2.0 35.0 78-79 22.165610563580806 31.5 2.0 35.0 2.0 36.0 80-81 21.865067424850935 31.5 2.0 35.0 2.0 35.0 82-83 21.439103013612467 31.0 2.0 35.0 2.0 35.0 84-85 21.017624384294024 31.0 2.0 35.0 2.0 35.0 86-87 20.549233103413105 29.5 2.0 35.0 2.0 35.0 88-89 20.00381429585336 29.0 2.0 34.5 2.0 35.0 90-91 19.606211671107825 29.0 2.0 34.0 2.0 35.0 92-93 19.013344723096996 26.5 2.0 34.0 2.0 35.0 94-95 18.48255836191399 25.0 2.0 34.0 2.0 35.0 96-97 17.84866914011534 23.5 2.0 34.0 2.0 35.0 98-99 17.028455284552848 10.0 2.0 33.5 2.0 35.0 100-101 14.345804699552485 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29160513272285726 1101 2 0.2945778744623482 1101 3 0.2845394242917578 1101 4 0.18005937366831404 1101 5 0.11634525102427062 1101 6 0.21925432481233997 1101 7 0.15678173025757047 1101 8 0.0546326797991199 1101 9 -0.027346803753658833 1101 10-11 0.09993312620016326 1101 12-13 0.3584246274337346 1101 14-15 -0.0021790503272924866 1101 16-17 0.2223133667831405 1101 18-19 0.060882165774302166 1101 20-21 0.14953723113441697 1101 22-23 0.18328000810131329 1101 24-25 0.08817053777559636 1101 26-27 0.2205446131674833 1101 28-29 0.3438928760381046 1101 30-31 0.19836102469385963 1101 32-33 0.1483365660423317 1101 34-35 0.11739683875402562 1101 36-37 0.13871151057750808 1101 38-39 -0.19043429452413108 1101 40-41 0.012833554218666166 1101 42-43 0.23386893445618995 1101 44-45 0.8809281871072159 1101 46-47 0.25219659957445373 1101 48-49 -0.08530381657233477 1101 50-51 -0.30735608435539774 1101 52-53 -0.30907305544434394 1101 54-55 -0.12377835372619117 1101 56-57 -0.38511553291398926 1101 58-59 -0.27229595844540455 1101 60-61 -0.7541478574021134 1101 62-63 -0.789061516161496 1101 64-65 -0.7952099989299697 1101 66-67 -1.3157032926732128 1101 68-69 -1.1634398253021914 1101 70-71 -0.7652241201553522 1101 72-73 -0.5079364528648824 1101 74-75 -0.3312681221744711 1101 76-77 -0.3120942552170831 1101 78-79 -0.6780458954610147 1101 80-81 -0.7872553267155276 1101 82-83 -0.5832105814292525 1101 84-85 -0.993874608028694 1101 86-87 -1.0621773833173158 1101 88-89 -0.9145608658546145 1101 90-91 -1.0715343043661214 1101 92-93 -1.497743040699163 1101 94-95 -1.392622639150467 1101 96-97 -0.7744412795815734 1101 98-99 -0.05837847621534209 1101 100-101 0.20017465593898365 1102 1 -0.05892118306661587 1102 2 0.17089366393602745 1102 3 0.015065740081233514 1102 4 -0.008624836858004414 1102 5 -0.0707600121336256 1102 6 -0.10864041202976438 1102 7 0.06388699341546555 1102 8 0.08515899558859275 1102 9 0.3734426699305544 1102 10-11 0.3216436525159594 1102 12-13 0.2638193642758395 1102 14-15 0.3107156865148113 1102 16-17 0.2616554720463 1102 18-19 0.23654006051114607 1102 20-21 0.26401091534494014 1102 22-23 0.25130632389078755 1102 24-25 0.26619685356507006 1102 26-27 0.04264987632538464 1102 28-29 0.11441919182757232 1102 30-31 0.019676814167546297 1102 32-33 -0.2365318550103055 1102 34-35 -0.21010316124598205 1102 36-37 -0.022077963106696075 1102 38-39 -0.20306587347149474 1102 40-41 -0.5566401299918695 1102 42-43 -1.0023152760701208 1102 44-45 -0.9628876023664645 1102 46-47 -0.8953034004255471 1102 48-49 -1.0599090797302289 1102 50-51 -1.2630139790922392 1102 52-53 -1.073020423865394 1102 54-55 -1.2535151958314508 1102 56-57 -0.9941944802824096 1102 58-59 -0.8961117479190897 1102 60-61 -0.6655952258231679 1102 62-63 -0.729587831950969 1102 64-65 -0.6044205252457573 1102 66-67 -0.31122960846268555 1102 68-69 -0.008439825302193782 1102 70-71 0.08872324826569766 1102 72-73 0.3108793366087994 1102 74-75 0.07702135150973533 1102 76-77 -0.14946267626971732 1102 78-79 -0.1667301059873303 1102 80-81 -0.19778164250500296 1102 82-83 0.10178941857074975 1102 84-85 0.04388854986604329 1102 86-87 0.12940156405110415 1102 88-89 0.5284654499348598 1102 90-91 0.5432025377391376 1102 92-93 0.9608095908797836 1102 94-95 0.8410615713758531 1102 96-97 0.5137166151552677 1102 98-99 0.9175425764162348 1102 100-101 0.3591220243600368 1103 1 0.41870257930962396 1103 2 0.38723029759939465 1103 3 0.2683330668139021 1103 4 0.12023654928061234 1103 5 0.10790335420301034 1103 6 0.1781912711385516 1103 7 0.1860157062867529 1103 8 0.22654513420245337 1103 9 0.278541679831541 1103 10-11 0.28909414756546425 1103 12-13 0.2499827306124729 1103 14-15 0.38195331027718993 1103 16-17 -0.022527696270529418 1103 18-19 0.4542380803131181 1103 20-21 0.4038624004934519 1103 22-23 0.6235092941878122 1103 24-25 0.5660235862383374 1103 26-27 0.7859172030580552 1103 28-29 0.5489736472731224 1103 30-31 0.19051839832595618 1103 32-33 0.3836414123164289 1103 34-35 0.6159116902391659 1103 36-37 1.2343081755071594 1103 38-39 1.642082641379993 1103 40-41 1.839201454166549 1103 42-43 1.7720659120486886 1103 44-45 1.5046123976335366 1103 46-47 2.1318995698714787 1103 48-49 1.658234484626206 1103 50-51 1.618099882293901 1103 52-53 1.8144548236593572 1103 54-55 1.9592075764457775 1103 56-57 2.3422164108066994 1103 58-59 1.9919823114868507 1103 60-61 2.0010631900184173 1103 62-63 1.819224049237146 1103 64-65 1.092361652972066 1103 66-67 0.795552569755138 1103 68-69 1.1603225509354296 1103 70-71 1.3889707730181762 1103 72-73 1.3060030989850375 1103 74-75 1.561749074282016 1103 76-77 1.6558838583837456 1103 78-79 1.2195570227255423 1103 80-81 0.9839015258118273 1103 82-83 1.1489676363925305 1103 84-85 1.0064380548165381 1103 86-87 1.4545500789025958 1103 88-89 0.9390347568655564 1103 90-91 0.5989451119965672 1103 92-93 0.800537313652061 1103 94-95 0.4865071159303085 1103 96-97 0.29713245673942623 1103 98-99 0.1555871308716803 1103 100-101 0.14892400455805443 1104 1 -0.010509968113339596 1104 2 0.12369740225378933 1104 3 0.12777602045506598 1104 4 0.057777032300876385 1104 5 0.17984746450188993 1104 6 0.2912661300263082 1104 7 0.025055217714530897 1104 8 0.02403750026149254 1104 9 -0.07604331137785891 1104 10-11 -0.010248870848528213 1104 12-13 -0.009848860023225825 1104 14-15 0.11786521922508797 1104 16-17 0.23672556550424417 1104 18-19 0.08151669602516165 1104 20-21 -0.012858243533564462 1104 22-23 0.175582024825367 1104 24-25 0.18187442365852036 1104 26-27 0.10330408193286189 1104 28-29 0.01843788341636099 1104 30-31 0.08075158052267994 1104 32-33 0.3238653412513841 1104 34-35 0.40898562380075276 1104 36-37 0.6719874574540512 1104 38-39 0.6522612293322467 1104 40-41 0.7901822999146759 1104 42-43 0.8784090229952994 1104 44-45 0.4812479116522326 1104 46-47 0.1909816463034275 1104 48-49 -0.1629230984218175 1104 50-51 0.43275704894514533 1104 52-53 0.5666524733308691 1104 54-55 0.682419383607801 1104 56-57 -0.007348685889887463 1104 58-59 0.5023228315201642 1104 60-61 0.08045617604598831 1104 62-63 0.38499160730136595 1104 64-65 0.19814956821218743 1104 66-67 0.09526571864012112 1104 68-69 0.09029849245481714 1104 70-71 0.24783539779841135 1104 72-73 -0.022742158718301653 1104 74-75 0.3055493888929135 1104 76-77 -0.0010514613164467335 1104 78-79 -0.6120104798191086 1104 80-81 -0.721613418205937 1104 82-83 -0.6705937589993418 1104 84-85 -0.38113481461993715 1104 86-87 -1.1964862864161816 1104 88-89 -1.6115111855791575 1104 90-91 -1.2473582099244105 1104 92-93 -1.4790268577183454 1104 94-95 -1.162863662269011 1104 96-97 -1.779507683910154 1104 98-99 -1.7595835918080631 1104 100-101 -2.5671396578829544 1105 1 0.06920667081466547 1105 2 0.08710370959813218 1105 3 -0.08986576676808511 1105 4 0.1616263046945079 1105 5 -0.007495171950978374 1105 6 -0.17060388234939694 1105 7 0.13632991578989362 1105 8 -0.034384383406838026 1105 9 -0.14534728440734312 1105 10-11 -0.11311662145664769 1105 12-13 0.19736959258634101 1105 14-15 0.055943997017095626 1105 16-17 -0.20340160557927334 1105 18-19 -0.28737546460301644 1105 20-21 -0.1258292673034589 1105 22-23 0.05385198599124408 1105 24-25 -0.34399721036187003 1105 26-27 -0.2035601693367184 1105 28-29 -0.20448491776146938 1105 30-31 -0.288108573960308 1105 32-33 -0.18350674085505148 1105 34-35 0.19570734103712795 1105 36-37 0.5076252332403328 1105 38-39 0.3772537612316995 1105 40-41 0.3754146645286838 1105 42-43 0.2216002490440303 1105 44-45 0.686119246948607 1105 46-47 0.718634955738839 1105 48-49 0.9531845275757043 1105 50-51 0.7785727788986314 1105 52-53 0.4638060601528693 1105 54-55 0.648151470835213 1105 56-57 0.5266845151513806 1105 58-59 0.8573243251402722 1105 60-61 0.8427038609348223 1105 62-63 0.2826039488709462 1105 64-65 0.0947803879962521 1105 66-67 -0.03307892353117836 1105 68-69 -0.1438051221058423 1105 70-71 0.03244470945291411 1105 72-73 0.30696381149464713 1105 74-75 0.042512219089648084 1105 76-77 0.1323181456480924 1105 78-79 0.04918313602180291 1105 80-81 -0.005315889080343084 1105 82-83 0.5140496925433524 1105 84-85 0.18033832155554208 1105 86-87 0.45798603893695145 1105 88-89 0.40920745906727873 1105 90-91 0.7496865560039829 1105 92-93 1.0004785406514713 1105 94-95 1.0127967311932018 1105 96-97 0.33506364711873715 1105 98-99 0.04223435723815072 1105 100-101 -0.04211085235229284 1106 1 0.14536453121910142 1106 2 0.2810977455686796 1106 3 0.29914737273429637 1106 4 0.06759965293791481 1106 5 0.1375052939888235 1106 6 0.0909514247049259 1106 7 0.28154005463995446 1106 8 0.1661794037518618 1106 9 0.11497328217544833 1106 10-11 0.3351640606792259 1106 12-13 -0.06990512552007999 1106 14-15 -0.022345537974985064 1106 16-17 0.11782894143405542 1106 18-19 0.41281557071522457 1106 20-21 0.5344190786102487 1106 22-23 0.5785002014418126 1106 24-25 0.5512478739732316 1106 26-27 0.6330580395906864 1106 28-29 0.3207457224398169 1106 30-31 -0.35287420624062094 1106 32-33 -0.35158287541846533 1106 34-35 -0.43056234491944423 1106 36-37 -0.17809837126996797 1106 38-39 -0.312453628573536 1106 40-41 0.05091089041629715 1106 42-43 0.08518472392987775 1106 44-45 -0.0030406635909514534 1106 46-47 -0.5865789106296262 1106 48-49 -0.8488376511587994 1106 50-51 -0.7883711219493854 1106 52-53 -1.1318979748858027 1106 54-55 -0.8731070325661427 1106 56-57 -1.0957761129354715 1106 58-59 -0.8134076662864373 1106 60-61 -0.914727878884392 1106 62-63 -0.651628648277498 1106 64-65 -0.6758490966743267 1106 66-67 -1.1741887921361567 1106 68-69 -1.9532357436695378 1106 70-71 -1.9128073639791978 1106 72-73 -2.393355357268753 1106 74-75 -2.0828255872657735 1106 76-77 -1.5645647170860428 1106 78-79 -1.4144852080281467 1106 80-81 -1.2049244996478592 1106 82-83 -1.814333030408843 1106 84-85 -1.6569787970727283 1106 86-87 -1.7678433339080755 1106 88-89 -2.356075366391668 1106 90-91 -2.1176137887914734 1106 92-93 -2.4435271438140944 1106 94-95 -1.8873057755629254 1106 96-97 -1.4658752215794237 1106 98-99 -0.9098553827674394 1106 100-101 -1.4040412409460856 1107 1 0.13269172015919395 1107 2 0.10153882522634916 1107 3 0.07087219169413572 1107 4 0.1152461308839321 1107 5 0.1677883749631519 1107 6 0.10329507184120246 1107 7 0.023886993415466407 1107 8 0.014352543975689969 1107 9 0.26828137960796994 1107 10-11 0.12285332993531739 1107 12-13 -0.08730966798222539 1107 14-15 0.10450600909545216 1107 16-17 -0.2190703344053162 1107 18-19 0.1474271572853354 1107 20-21 -0.008166504009892606 1107 22-23 0.013161162600468401 1107 24-25 -0.12065798514461079 1107 26-27 0.056198263422153616 1107 28-29 -0.25364532430145914 1107 30-31 0.04887036255464494 1107 32-33 -0.13733830662320656 1107 34-35 -0.020506387052428465 1107 36-37 -0.09683602762282817 1107 38-39 0.20935348136720933 1107 40-41 0.3991663216210384 1107 42-43 -0.2232830180056098 1107 44-45 0.4602575589238569 1107 46-47 0.7292127286067149 1107 48-49 0.43226833962460987 1107 50-51 0.24561505316582455 1107 52-53 0.36439893097331577 1107 54-55 0.2113235138459686 1107 56-57 0.5115313261692052 1107 58-59 1.0493721230486557 1107 60-61 1.0882757419187676 1107 62-63 0.45242829708128696 1107 64-65 0.4540472166897267 1107 66-67 0.020141359279250537 1107 68-69 0.5083343682461958 1107 70-71 0.9076748611689247 1107 72-73 0.5199922398346075 1107 74-75 0.5829084482839306 1107 76-77 0.42037603340770247 1107 78-79 0.3240763456255742 1107 80-81 0.5897183574949985 1107 82-83 0.6309023217965581 1107 84-85 0.7795337111563647 1107 86-87 0.7960144672769154 1107 88-89 0.32459448219292497 1107 90-91 0.4312670538681722 1107 92-93 0.39427733281526756 1107 94-95 0.2689647971823028 1107 96-97 -0.010476933231828411 1107 98-99 -0.11858645584182881 1107 100-101 -0.6128134595109316 1108 1 -0.10924376371177402 1108 2 -0.2532998844510672 1108 3 -0.09848264701554399 1108 4 0.050730001851675866 1108 5 -0.3564051734239442 1108 6 -0.01767267009428508 1108 7 -0.12933881303614925 1108 8 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-0.2014432426573407 2316 54-55 -0.25005881999252466 2316 56-57 0.1427014928719501 2316 58-59 0.09836811785272204 2316 60-61 -0.13007509159497843 2316 62-63 -0.01770863732009431 2316 64-65 0.2856801458951814 2316 66-67 0.5006160291212076 2316 68-69 0.7144125236910952 2316 70-71 0.5412400267891897 2316 72-73 0.43079544399135017 2316 74-75 0.7442528951338971 2316 76-77 0.8281212163477321 2316 78-79 0.7425651960260922 2316 80-81 0.7948358071594264 2316 82-83 1.0815545192418945 2316 84-85 0.8251469391277908 2316 86-87 0.5516163291517806 2316 88-89 1.0194553828207695 2316 90-91 1.1329340813632989 2316 92-93 0.8739807318193797 2316 94-95 1.5444843901677956 2316 96-97 1.6773072191821115 2316 98-99 1.4969553280940886 2316 100-101 1.4294575948298345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 6.0 5 27.0 6 72.0 7 143.0 8 273.0 9 472.0 10 918.0 11 1552.0 12 2302.0 13 3356.0 14 3978.0 15 5765.0 16 7946.0 17 10780.0 18 9513.0 19 5517.0 20 4242.0 21 4299.0 22 4457.0 23 4697.0 24 4804.0 25 4899.0 26 4559.0 27 4179.0 28 4702.0 29 5208.0 30 6049.0 31 7068.0 32 8445.0 33 10540.0 34 16574.0 35 33024.0 36 21660.0 37 20501.0 38 12083.0 39 684.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.3822228078154 21.896556853777856 15.968700989038545 12.752519349368196 2 16.877143913729125 26.216212769755888 38.84739797414764 18.059245342367348 3 18.68250035064753 35.98876227797636 32.941741506891816 12.386995864484293 4 10.246537384349274 24.481617006447117 33.54710389759413 31.724741711609482 5 8.276278267225955 44.34995473843918 29.539437056681074 17.834329937653795 6 20.7062593499252 41.03724670202639 16.854515163878688 21.401978784169724 7 19.102078631868388 46.645757100540166 17.50623674558753 16.745927522003917 8 27.649076281667156 32.52882502688069 17.670708332802093 22.15139035865006 9 29.650359755035083 17.531736216473508 18.975431259801358 33.84247276869005 10-11 23.436138700121973 32.968053412891685 20.901278798464933 22.694529088521413 12-13 28.773239098421794 33.45665186985892 24.08791386260431 13.682195169114975 14-15 24.33159511940127 31.601919260175354 29.922354111152195 14.144131509271181 16-17 14.75515737757869 36.27270563635282 29.4089522044761 19.56318478159239 18-19 13.804351059715511 28.902162780122314 41.59197446652982 15.701511693632359 20-21 17.569347936030326 26.713245700151724 42.61705319614618 13.100353167671772 22-23 16.438446402237155 29.558561659845555 42.138725621443356 11.864266316473932 24-25 14.475205276847491 30.96110364993285 42.9691617224555 11.594529350764157 26-27 13.64798547955512 31.563264464654235 43.2756865437218 11.513063512068843 28-29 12.869046328821792 31.695323341217374 43.02687585932834 12.408754470632486 30-31 13.792165512786186 32.35816620128946 42.596252332198574 11.25341595372578 32-33 12.73932315904445 32.74195810436933 43.03375704954122 11.484961687044994 34-35 12.3272503936874 33.04976506266058 43.01807880548548 11.604905738166533 36-37 12.663755458515283 32.58151914066235 42.05654543715934 12.698179963663023 38-39 12.118694311572568 32.65559822094533 42.13418560223721 13.091521865244896 40-41 11.79630589815295 32.95111647555824 42.16023358011679 13.092344046172022 42-43 12.090747009560483 32.82141513824342 41.695938867319896 13.391898984876208 44-45 12.130735787232414 32.97846569683679 40.703317906153444 14.187480609777348 46-47 13.052199874626801 32.857977666571045 38.9572774891361 15.132544969666062 48-49 12.610047323270852 32.95905378958038 37.86201664708145 16.56888224006732 50-51 12.643414361517882 33.629352900800505 36.822454136092496 16.90477860158911 52-53 13.131218680800242 34.18112113703305 35.91969534042562 16.767964841741083 54-55 13.053752746715631 33.5551957021604 34.96924953566161 18.42180201546236 56-57 14.32585284572371 33.89385628027281 33.52553489489132 18.25475597911216 58-59 13.805739354816767 34.16236069810892 34.183615993334335 17.848283953739976 60-61 14.745357875991857 35.1167277966433 33.09263847745982 17.04527584990501 62-63 14.621808373840834 35.318147714399274 33.11809627855274 16.941947633207153 64-65 14.748885719569039 35.601923980250085 33.022022049680864 16.62716825050002 66-67 15.030593355960445 35.036246591587734 32.58070206385606 17.352457988595766 68-69 15.130496520234507 34.19183137273241 32.95956499742791 17.71810710960517 70-71 14.978608364097756 34.0973962351571 33.0761661257654 17.847829274979755 72-73 14.713676421523731 33.2352959927237 33.245071392293156 18.80595619345941 74-75 13.832254005768222 33.69393571642625 33.01488388107347 19.45892639673205 76-77 12.9451798573384 32.851701651915896 34.68300728611388 19.520111204631828 78-79 12.706658561159031 32.59136763436792 34.733092151969764 19.968881652503285 80-81 12.809461570340144 32.28346456692913 34.45588532202705 20.451188540703665 82-83 12.642270719336063 32.21855163412031 33.69748685178335 21.44169079476027 84-85 13.173527967710191 31.65791336248031 33.436700964315854 21.731857705493645 86-87 13.117332828831433 31.72856380638181 33.09199436261641 22.06210900217035 88-89 13.41292597544205 31.330197522099816 32.4866784702399 22.77019803221823 90-91 13.515058890048529 31.115754389917804 33.01562536533936 22.353561354694303 92-93 13.587988105435663 31.003662318504805 33.02590829962569 22.38244127643384 94-95 13.771601146818726 30.72224501029714 32.964874957228076 22.54127888565605 96-97 13.99314794821005 30.78386282294332 32.75148985369254 22.471499375154085 98-99 14.301744602443591 30.656675245517356 32.21957040572792 22.82200974631113 100-101 14.697187693262407 29.89465272804167 32.68698237553104 22.72117720316488 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7269.0 1 4473.0 2 2013.0 3 2214.5 4 1628.0 5 1491.0 6 2762.5 7 5422.0 8 7567.5 9 7559.5 10 5222.0 11 2886.0 12 2438.0 13 2383.0 14 2180.0 15 1898.0 16 1640.5 17 1486.5 18 1445.5 19 1386.5 20 1373.5 21 1413.0 22 1477.0 23 1596.5 24 1739.0 25 1984.5 26 2435.0 27 2962.5 28 3195.0 29 3374.0 30 3878.5 31 4395.5 32 4866.0 33 5448.0 34 6067.0 35 6410.0 36 6371.5 37 6445.5 38 6128.0 39 5483.5 40 5327.5 41 5268.0 42 5289.0 43 5436.0 44 5575.5 45 5414.5 46 5051.5 47 5191.5 48 5680.5 49 5536.0 50 5012.5 51 4493.0 52 3753.0 53 3006.0 54 2447.0 55 2376.5 56 2640.5 57 2773.0 58 2542.5 59 2570.0 60 2773.0 61 2961.0 62 2448.0 63 1079.0 64 315.0 65 162.0 66 125.0 67 125.5 68 122.5 69 123.5 70 122.5 71 132.5 72 125.0 73 135.5 74 152.0 75 101.0 76 49.0 77 34.5 78 28.5 79 13.0 80 4.0 81 2.0 82 1.5 83 1.5 84 1.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008499823628659704 2 0.01699964725731941 3 0.008499823628659704 4 0.0 5 0.0 6 0.0012749735442989557 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.1249559071649264E-4 14-15 0.0 16-17 0.002124955907164926 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002974938270030897 26-27 0.018912107573767843 28-29 0.005737380949345301 30-31 0.0025499470885979115 32-33 0.0 34-35 0.009987292763675153 36-37 6.374867721494779E-4 38-39 0.002337451497881419 40-41 0.002124955907164926 42-43 0.0031874338607473895 44-45 0.0 46-47 6.374867721494779E-4 48-49 0.001062477953582463 50-51 0.004462407405046345 52-53 0.008499823628659704 54-55 0.007649841265793734 56-57 0.018062125210901874 58-59 0.026986940020994565 60-61 0.0033999294514638822 62-63 0.0231620193880977 64-65 0.024436992932396654 66-67 0.015087186940870977 68-69 0.03484927687750479 70-71 0.018487116392334857 72-73 0.006162372130778286 74-75 0.018912107573767843 76-77 0.02422449734168016 78-79 0.028899400337442996 80-81 0.013387222215139036 82-83 0.04079915341756658 84-85 0.0231620193880977 86-87 0.035486763649654265 88-89 0.025286975295262623 90-91 0.030811860653891434 92-93 0.027836922383860537 94-95 0.01678715166660292 96-97 0.017424638438752395 98-99 0.01296223103370605 100-101 0.012537239852273067 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 235299.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.26450601150026 #Duplication Level Percentage of deduplicated Percentage of total 1 78.17095384068595 29.91172933161637 2 11.163312452796658 8.54317272916587 3 4.01728197609845 4.611579309729323 4 1.940334977120263 2.969838375853701 5 1.052912168465947 2.0144581999923505 6 0.6619574392465236 1.5197684648043552 7 0.46536940779243857 1.2464991351429457 8 0.36874139233195613 1.1287765778860088 9 0.27433471056022035 0.9447553963255263 >10 1.574925585321427 11.590359500040375 >50 0.16660002665600426 4.597979591923468 >100 0.12328401972544316 8.118606538914317 >500 0.006664001066240171 1.5941419215551276 >1k 0.008885334754986894 6.271594864406564 >5k 0.0033320005331200853 10.336210523631635 >10k+ 0.0011106668443733618 4.600529539012065 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10825 4.600529539012065 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9476 4.0272164352589686 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8109 3.4462534902400774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6736 2.862740598132589 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2752 1.1695757313035755 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2414 1.0259287119792264 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 2315 0.9838545850173609 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1883 0.8002583946383113 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1631 0.693160616917199 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 1470 0.6247370367064883 RNA PCR Primer, Index 23 (95% over 23bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 0.5159392942596441 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1078 0.45814049358475817 No Hit ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 796 0.33829298042065625 RNA PCR Primer, Index 23 (96% over 25bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 714 0.3034437035431515 No Hit TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 580 0.24649488523113147 RNA PCR Primer, Index 23 (96% over 30bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 576 0.2447949205053995 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 573 0.24351994696110058 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 512 0.21759548489368846 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 481 0.2044207582692659 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 470 0.19974585527350305 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 409 0.17382139320609097 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 394 0.1674465254845962 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 369 0.15682174594877157 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 363 0.15427179886017367 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 363 0.15427179886017367 No Hit CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT 357 0.15172185177157574 RNA PCR Primer, Index 23 (96% over 28bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 353 0.1500218870458438 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 346 0.1470469487758129 No Hit TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC 313 0.1330222397885244 No Hit CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA 310 0.13174726624422545 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 305 0.1296223103370605 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 294 0.12494740734129767 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 281 0.11942252198266887 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 280 0.11899753080123587 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 259 0.11007271599114318 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 257 0.10922273362827721 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 251 0.1066727865396793 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 240 0.10199788354391646 No Hit CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG 238 0.10114790118105049 Illumina PCR Primer Index 1 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.499823628659706E-4 0.0 18-19 0.0 0.0 0.0 0.0012749735442989557 0.0 20-21 0.0 0.0 0.0 0.002974938270030897 0.0 22-23 0.0 0.0 0.0 0.010837275126541125 0.0 24-25 0.0 0.0 0.0 0.029111895928159492 0.0 26-27 0.0 0.0 0.0 0.0690610669828601 0.0 28-29 0.0 0.0 0.0 0.15405930326945716 0.0 30-31 0.0 0.0 0.0 0.35401765413367675 0.0 32-33 0.0 0.0 0.0 0.5561009609050612 0.0 34-35 0.0 0.0 0.0 0.8028083417269092 0.0 36-37 0.0 0.0 0.0 1.1247391616623954 0.0 38-39 0.0 0.0 0.0 1.525293350162984 0.0 40-41 0.0 0.0 0.0 1.9377472917436962 0.0 42-43 0.0 0.0 0.0 2.266690466172827 0.0 44-45 0.0 0.0 0.0 2.59669611855554 0.0 46-47 0.0 0.0 0.0 2.9400889931533922 0.0 48-49 0.0 0.0 0.0 3.302393975325012 0.0 50-51 0.0 0.0 0.0 3.6632114883616165 0.0 52-53 0.0 0.0 0.0 3.9728175640355463 0.0 54-55 0.0 0.0 0.0 4.246936876059822 0.0 56-57 0.0 0.0 0.0 4.551868048737989 0.0 58-59 0.0 0.0 0.0 4.857861699369739 0.0 60-61 0.0 0.0 0.0 5.198917122469709 0.0 62-63 0.0 0.0 0.0 5.508735693734355 0.0 64-65 0.0 0.0 0.0 5.814729344366104 0.0 66-67 0.0 0.0 0.0 6.129222818626514 0.0 68-69 0.0 0.0 0.0 6.474528153540813 0.0 70-71 0.0 0.0 0.0 6.842145525480346 0.0 72-73 0.0 0.0 0.0 7.211462862145611 0.0 74-75 0.0 0.0 0.0 7.554855736743463 0.0 76-77 0.0 0.0 0.0 7.859361918240197 0.0 78-79 0.0 0.0 0.0 8.208279678196678 0.0 80-81 0.0 0.0 0.0 8.586309334081317 0.0 82-83 0.0 0.0 0.0 8.954351697202283 0.0 84-85 0.0 0.0 0.0 9.323881529458262 0.0 86-87 0.0 0.0 0.0 9.66387447460465 0.0 88-89 0.0 0.0 0.0 10.04211662608001 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 15 6.6179765E-4 94.97598 5 TATCACG 25 5.2676627E-5 75.99694 2 CGGTATC 20 0.0020723513 71.24713 2 CGTCACT 20 0.0020723513 71.24713 1 AACGCGT 20 0.0020740998 71.23199 6 TCACGCA 30 1.3007104E-4 63.31732 4 ATCACGC 30 1.3007104E-4 63.31732 3 CTAACGC 25 0.005021349 56.985588 4 CGCGTAC 25 0.005021349 56.985588 8 ATGGGTT 35 2.786854E-4 54.27199 5 GGTATCA 3700 0.0 47.754833 1 CACGCAG 55 4.9586222E-5 43.170902 5 CACGAAC 30 8.264507E-5 39.598576 76-77 ACGCAGT 50 0.0016152093 37.99039 8 GTAAGCG 25 0.001616853 37.99039 94-95 GGGGCGT 50 0.0016152093 37.99039 7 GTATCAA 6060 0.0 36.99521 1 TGGGATC 125 5.6261342E-9 34.191357 8 TACCTCC 160 5.2750693E-11 32.654934 2 ATAGACG 150 9.495125E-10 31.658663 3 >>END_MODULE