FastQCFastQC Report
Thu 26 May 2016
SRR938271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626346
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149680.5699172919333554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120210.45770816183397006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75280.2866339773967329No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA46550.17724245015698617No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45430.17297797015320907No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42440.16159333157169695No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40740.1551204601373924No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC39000.14849528584581012No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35640.13570184583447878No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA33240.12656367439781355No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32570.12401260153841115No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31430.11967197010599517No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA29390.11190452438482971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59550.060.646171
GTATCAA112400.044.312211
GTGGTAT22800.040.1749761
TGGTATC23100.038.0568352
TATCAAC145050.034.2350922
ATCAACG145950.034.023983
TCAACGC147900.033.543264
CAACGCA149500.033.0253375
AACGCAG154750.031.9915686
CGCAGAG175550.028.0890488
ACGCAGA175200.028.0638227
AGGTAAG17200.025.6937482
GCAGAGT189400.025.4074889
TAAGGTG19350.025.294685
GTAAGGT19900.024.1179964
GTACATG111500.023.9180741
GAGTACT122850.023.68354212-13
TACATGG112200.023.2092252
CAGAGTA180650.022.73291610-11
GTACTTT127100.022.68669714-15
Produced by FastQC (version 0.11.5)