FastQCFastQC Report
Thu 26 May 2016
SRR938271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626346
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141700.5395328719064434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121550.46281030755277486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76710.29207880454441265No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA45460.17309219729616737No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45010.17137879015179264No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA44110.1679519758630432No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC43400.16524860014636306No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42080.16022260585619716No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG35140.13379806011850687No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34000.12945742868609086No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA32470.12363184439521677No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31600.12031925724942563No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA29440.11209490295642692No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA28560.10874424009631634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60300.060.7252581
GTATCAA110100.043.0629541
TGGTATC22000.035.4500732
ATCAACG143050.032.9406473
TATCAAC143800.032.904872
GTGGTAT24850.032.5325781
TCAACGC145100.032.4740144
CAACGCA147100.032.0001685
AACGCAG151100.031.1809146
TAAGGTG18250.030.2221165
AGGTAAG16100.028.6511342
ACGCAGA172000.027.3092337
CGCAGAG174850.026.8641018
GTAAGGT19350.026.7839894
GTGTAAG10250.025.5173051
AAGGTAA18750.024.094521
GCAGAGT192800.024.06749
GGTAAGG21000.023.3219173
GAGTACT125900.022.95495612-13
GTACATG110050.022.816021
Produced by FastQC (version 0.11.5)