FastQCFastQC Report
Thu 26 May 2016
SRR938270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626809
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148400.5649440062067703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122440.4661168741236991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74170.2823577960940441No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47640.1813607308334942No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA44960.1711582379990323No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA41700.1587477429839779No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC40900.1557022227348848No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39090.14881173317131166No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35430.1348784780317107No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA33310.126807849371614No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG31760.12090715388899612No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30820.11732866759631172No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30080.11451156136590061No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA26280.10004534018270836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.058.7266851
GTATCAA111350.046.2698141
ATCAACG141250.036.2038463
TATCAAC141700.036.086812
TGGTATC22350.035.9348032
TCAACGC144050.035.5661134
CAACGCA145550.035.134275
GTGGTAT23350.034.9360241
AACGCAG150500.033.9432336
ACGCAGA171200.029.6890167
CGCAGAG173750.029.2532968
GCAGAGT191950.026.0579079
AGGTAAG16450.025.1338772
TAAGGTA9150.024.4122434
TAAGGTG20200.023.7629815
GAGTACT123550.023.06810412-13
GTACATG108000.022.9690911
GTAAGGT19700.022.9186134
TACATGG108100.022.8603952
CAGAGTA180450.022.74257310-11
Produced by FastQC (version 0.11.5)