FastQCFastQC Report
Thu 26 May 2016
SRR938270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626809
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146110.5562262044937413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123950.47186529359386237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77810.2962149132274178No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47160.17953341868403833No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC43760.16658995762539264No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA43350.1650291284977324No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA42640.1623262292766623No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40940.15585449874733945No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA36060.13727682522787155No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG33400.12715047039963698No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA32670.12437143317233952No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32570.12399074314120288No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA29080.11070466105453423No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA28160.10720231276807717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62100.060.3533131
GTATCAA114200.043.301241
GTGGTAT24000.034.2413251
TGGTATC23400.034.10432
ATCAACG144600.033.8345073
TATCAAC146050.033.7607042
TCAACGC148100.033.1292344
CAACGCA149950.032.783855
AACGCAG155100.031.8177786
ACGCAGA178200.027.4800227
CGCAGAG179200.027.2206558
TAAGGTA8650.026.3567124
GTGTAAG11450.024.8920961
TAAGGTG19250.024.6737615
GCAGAGT195300.024.6118569
GTAAGGT19950.024.0460974
TGTAAGG10850.023.2039452
AGGTAAG18300.022.8426912
GAGTACT126650.022.76405312-13
CAGAGTA186150.022.49186110-11
Produced by FastQC (version 0.11.5)