##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938270_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2626809 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4835566651401 33.0 31.0 34.0 31.0 34.0 2 32.713967022345365 34.0 31.0 34.0 31.0 34.0 3 32.67237130678325 34.0 31.0 34.0 31.0 34.0 4 36.20510018048515 37.0 35.0 37.0 35.0 37.0 5 36.167272915541254 37.0 35.0 37.0 35.0 37.0 6 36.23129470014759 37.0 37.0 37.0 35.0 37.0 7 36.19189594675517 37.0 37.0 37.0 35.0 37.0 8 36.18479836181466 37.0 37.0 37.0 35.0 37.0 9 37.88520710870109 39.0 38.0 39.0 35.0 39.0 10-11 37.85306811420244 39.0 38.0 39.0 35.0 39.0 12-13 37.79202294494955 39.0 38.0 39.0 35.0 39.0 14-15 39.055693619140186 40.0 38.0 41.0 36.0 41.0 16-17 38.98959231523875 40.0 38.0 41.0 36.0 41.0 18-19 38.99430468678918 40.0 38.5 41.0 36.0 41.0 20-21 38.99920321576484 40.0 38.5 41.0 35.5 41.0 22-23 39.02335971134559 40.0 39.0 41.0 35.0 41.0 24-25 38.96083194476644 40.0 38.0 41.0 35.0 41.0 26-27 38.782468957583134 40.0 38.0 41.0 35.0 41.0 28-29 38.699277145768875 40.0 38.0 41.0 35.0 41.0 30-31 38.4563919569333 40.0 38.0 41.0 34.0 41.0 32-33 38.23071015060478 40.0 38.0 41.0 34.0 41.0 34-35 38.01135408017865 40.0 37.0 41.0 33.5 41.0 36-37 37.9119648592646 40.0 37.0 41.0 33.0 41.0 38-39 37.8471023207245 40.0 37.0 41.0 33.0 41.0 40-41 37.69889531366765 40.0 37.0 41.0 33.0 41.0 42-43 37.48938217434157 40.0 37.0 41.0 33.0 41.0 44-45 37.211727993927234 40.0 36.5 41.0 32.0 41.0 46-47 36.821663090083824 40.0 36.0 41.0 31.0 41.0 48-49 36.39907031687496 39.0 35.5 41.0 30.0 41.0 50-51 36.05663373317208 39.0 35.0 41.0 29.5 41.0 52-53 35.56079334279728 39.0 35.0 40.0 28.0 41.0 54-55 35.22586663133863 38.0 35.0 40.0 27.0 41.0 56-57 34.91988587674247 38.0 34.0 40.0 27.0 41.0 58-59 34.507299160312 37.5 34.0 40.0 26.0 41.0 60-61 34.06800151819184 37.0 33.5 40.0 25.5 41.0 62-63 33.6165802309951 36.5 33.0 39.0 23.5 41.0 64-65 33.15632826749109 36.0 33.0 39.0 22.5 40.0 66-67 32.62458233544959 35.0 32.0 38.5 21.5 40.0 68-69 32.0815099232567 35.0 31.0 38.0 19.5 40.0 70-71 31.47714489329068 35.0 31.0 37.0 18.0 39.5 72-73 31.01069777056497 34.5 30.5 36.5 15.5 39.0 74-75 30.465706109580104 34.0 30.0 36.0 11.0 39.0 76-77 29.28733208238589 32.5 28.5 35.0 10.0 37.0 78-79 29.994926353610026 34.0 30.0 35.0 7.5 37.0 80-81 29.818547903559033 34.0 30.0 35.0 7.0 37.0 82-83 29.373079276034154 34.0 29.0 35.0 2.0 36.5 84-85 28.88055222134537 34.0 29.0 35.0 2.0 36.0 86-87 28.35259168062847 33.5 28.5 35.0 2.0 35.5 88-89 27.714452021445034 33.0 26.5 35.0 2.0 35.0 90-91 27.178546099088287 33.0 26.0 35.0 2.0 35.0 92-93 26.53832787233484 32.5 25.0 35.0 2.0 35.0 94-95 25.78822042257355 32.0 23.5 34.0 2.0 35.0 96-97 24.9499303146898 32.0 19.0 34.0 2.0 35.0 98-99 23.889266977538146 31.0 4.5 34.0 2.0 35.0 100-101 20.384403662390376 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.0023053563682111644 1101 2 -0.007121787456874529 1101 3 0.06166733467620844 1101 4 0.015993512112750352 1101 5 0.015271971392635919 1101 6 0.017436310430788637 1101 7 0.067403566037612 1101 8 0.04184756013151514 1101 9 -0.03963208126155138 1101 10-11 0.025035375530379156 1101 12-13 0.06504422552927736 1101 14-15 0.19735593210919689 1101 16-17 0.20387413691425138 1101 18-19 0.20703867355168626 1101 20-21 0.1533297592875229 1101 22-23 0.036586585269304805 1101 24-25 0.13872422518173266 1101 26-27 0.1534444269046631 1101 28-29 0.19579144532437454 1101 30-31 0.15646667028276084 1101 32-33 0.15302720838280237 1101 34-35 0.1981329362927866 1101 36-37 0.186281573517185 1101 38-39 0.21660293478667114 1101 40-41 0.27880311352618037 1101 42-43 0.2983679812991298 1101 44-45 0.26461534461603975 1101 46-47 0.2032778352907414 1101 48-49 0.38766019890841363 1101 50-51 0.5317631657784503 1101 52-53 0.47090696595363823 1101 54-55 0.4633685074931577 1101 56-57 0.6681341762278592 1101 58-59 0.8017381353216066 1101 60-61 0.749517466931664 1101 62-63 0.6474577679120515 1101 64-65 0.6503642622477486 1101 66-67 0.8349030919907889 1101 68-69 0.9500357240736079 1101 70-71 0.8259647876617713 1101 72-73 0.9005904050097051 1101 74-75 0.79418240213273 1101 76-77 0.8472815401647154 1101 78-79 0.8108833920659286 1101 80-81 1.063967018005048 1101 82-83 1.2100565829227534 1101 84-85 1.2838992195578491 1101 86-87 1.477586318116849 1101 88-89 1.540044200142514 1101 90-91 1.512153107123524 1101 92-93 1.8886277036370664 1101 94-95 1.999865938944584 1101 96-97 1.9971912740402153 1101 98-99 2.1763975294546754 1101 100-101 2.5110090650004686 1102 1 0.11504063867081271 1102 2 0.0893900519344939 1102 3 0.21874039875481088 1102 4 0.14799237441670243 1102 5 0.027650027015909018 1102 6 0.08658501194810242 1102 7 0.10401706282768686 1102 8 0.12186145240639945 1102 9 0.12798053710334045 1102 10-11 0.2222697873531274 1102 12-13 0.14681129193745335 1102 14-15 0.36111518556388233 1102 16-17 0.3055258277859849 1102 18-19 0.38060813686098527 1102 20-21 0.3293977037338962 1102 22-23 0.2691024414242804 1102 24-25 0.25885293188446923 1102 26-27 0.19237259339525536 1102 28-29 0.21220677403726995 1102 30-31 0.1679629147248889 1102 32-33 0.29702508777928216 1102 34-35 0.26109512531285617 1102 36-37 0.30473391991854726 1102 38-39 0.3760523905113402 1102 40-41 0.267224540909055 1102 42-43 0.37192486803645863 1102 44-45 0.3779290004513811 1102 46-47 0.34621222041246824 1102 48-49 0.42724418525605046 1102 50-51 0.5415731821473528 1102 52-53 0.5265747432283447 1102 54-55 0.5406125549048966 1102 56-57 0.5830507645328069 1102 58-59 0.563010323296858 1102 60-61 0.5911875195984777 1102 62-63 0.6416728350322458 1102 64-65 0.5696067075977069 1102 66-67 0.6227749231783619 1102 68-69 0.8304303962507475 1102 70-71 0.9978125182677253 1102 72-73 0.86273811588012 1102 74-75 1.1329927358182061 1102 76-77 1.1200425233817626 1102 78-79 1.2418526200965907 1102 80-81 1.2893408324212814 1102 82-83 1.3051084757935776 1102 84-85 1.358349526619687 1102 86-87 1.410243322106524 1102 88-89 1.6306772660785747 1102 90-91 1.7038370907623737 1102 92-93 1.9906545633250907 1102 94-95 1.9705172505842974 1102 96-97 2.247430712622041 1102 98-99 2.4511936922458233 1102 100-101 2.6526358736082614 1103 1 0.26575161849022066 1103 2 0.29052982252647297 1103 3 0.3544900721777111 1103 4 0.24153440868912668 1103 5 0.13850318077038537 1103 6 0.16507454055361137 1103 7 0.15248286640560593 1103 8 0.15563249818006142 1103 9 0.2636359272225377 1103 10-11 0.35922994384579 1103 12-13 0.3012167233606675 1103 14-15 0.42640769510786214 1103 16-17 0.4358604894565161 1103 18-19 0.5074574003277519 1103 20-21 0.4786547821410423 1103 22-23 0.5088812942079528 1103 24-25 0.5310334095131068 1103 26-27 0.4429522815113742 1103 28-29 0.46144678011721396 1103 30-31 0.38303822020940004 1103 32-33 0.39688773815343836 1103 34-35 0.568920749664386 1103 36-37 0.5219813761417953 1103 38-39 0.5295852607991023 1103 40-41 0.66108323931482 1103 42-43 0.649682709741839 1103 44-45 0.5371242660196813 1103 46-47 0.6097909987387595 1103 48-49 0.5840107746016017 1103 50-51 0.5942724822920766 1103 52-53 0.7552078996703457 1103 54-55 0.7925667305758424 1103 56-57 0.7087025419272877 1103 58-59 0.8940055188135574 1103 60-61 0.9399155761508382 1103 62-63 0.8917877152726206 1103 64-65 0.797697404611263 1103 66-67 0.8463435462235864 1103 68-69 0.8508272011922458 1103 70-71 0.928828751297285 1103 72-73 0.8348340028811378 1103 74-75 0.8011120923126285 1103 76-77 0.6786147040796777 1103 78-79 1.0287405220948251 1103 80-81 1.2786857176621993 1103 82-83 1.3708899147594593 1103 84-85 1.43744771122552 1103 86-87 1.4136833409631855 1103 88-89 1.7367746201982932 1103 90-91 1.7207366019430914 1103 92-93 2.0946315344078705 1103 94-95 1.9931212320712852 1103 96-97 2.0448543332330793 1103 98-99 2.35421901876785 1103 100-101 2.6808989460544055 1104 1 0.0699653972795673 1104 2 0.05877994444189483 1104 3 0.10600138584918284 1104 4 0.030602433913884397 1104 5 0.010178151474889319 1104 6 0.038980583342144826 1104 7 -0.0033051527094585254 1104 8 0.008045627973650937 1104 9 -0.04071547876257142 1104 10-11 -0.014497104034951747 1104 12-13 -0.05522757063965855 1104 14-15 -0.007244262420222469 1104 16-17 0.07248869475810693 1104 18-19 0.07623301151581074 1104 20-21 0.09749129137438928 1104 22-23 0.09809673168344091 1104 24-25 0.01612492364296969 1104 26-27 0.08053572297088607 1104 28-29 0.026644481981705326 1104 30-31 0.0783312110074732 1104 32-33 0.15673961787840796 1104 34-35 0.07093941445783969 1104 36-37 0.06979171154642927 1104 38-39 0.151191020139251 1104 40-41 0.050296727734647106 1104 42-43 0.03551219998820443 1104 44-45 0.01781190876813099 1104 46-47 0.048528599363727665 1104 48-49 -0.03871052078056891 1104 50-51 0.13586592678257148 1104 52-53 0.06306283265067236 1104 54-55 0.06664989876979632 1104 56-57 0.2075166012215348 1104 58-59 0.12481925353037582 1104 60-61 0.12480796838993768 1104 62-63 0.12762663258413198 1104 64-65 0.30582516560771467 1104 66-67 0.1495161712992612 1104 68-69 0.420112152334589 1104 70-71 0.434039889712853 1104 72-73 0.48845757579451643 1104 74-75 0.30290303580672173 1104 76-77 0.27935363483570086 1104 78-79 0.41285464447483733 1104 80-81 0.7125411327354811 1104 82-83 0.7082386342298896 1104 84-85 0.9091476218871684 1104 86-87 1.0989919958045213 1104 88-89 1.1490989366962943 1104 90-91 1.1216555617997095 1104 92-93 1.4079585406026887 1104 94-95 1.6097453642443078 1104 96-97 1.9177844560076736 1104 98-99 1.9956717334841727 1104 100-101 2.2685678675265564 1105 1 0.10970303176880236 1105 2 0.06803627513468058 1105 3 0.15748753310261776 1105 4 0.08636054771728396 1105 5 0.05142192026377046 1105 6 0.07200698499045899 1105 7 0.00843096264330967 1105 8 0.07974469623258784 1105 9 0.055740312975430584 1105 10-11 0.006320266371275807 1105 12-13 -0.008759502440597089 1105 14-15 0.07549636377604685 1105 16-17 0.09356293348145073 1105 18-19 0.10935148662210281 1105 20-21 0.06147802070140784 1105 22-23 0.06981113039991271 1105 24-25 0.06291834829784904 1105 26-27 0.00465056291425725 1105 28-29 0.008342727778227754 1105 30-31 -0.030255901369322658 1105 32-33 -0.024418614819190054 1105 34-35 0.10513540604211613 1105 36-37 0.17609573477857055 1105 38-39 0.12988319604219356 1105 40-41 0.22070837017395206 1105 42-43 0.18058353348401823 1105 44-45 0.28702381439540403 1105 46-47 0.3305997955808877 1105 48-49 0.29954226667155837 1105 50-51 0.35373727914663533 1105 52-53 0.3763789829701878 1105 54-55 0.442528376319288 1105 56-57 0.42411570703556123 1105 58-59 0.48575476824368735 1105 60-61 0.49600022442248815 1105 62-63 0.5223103201911385 1105 64-65 0.5058912649769027 1105 66-67 0.5857794161191023 1105 68-69 0.5899506412776176 1105 70-71 0.8101870282391168 1105 72-73 0.7031765083350585 1105 74-75 0.7220545267315153 1105 76-77 0.836268185223922 1105 78-79 0.9379326423352232 1105 80-81 1.129064674815016 1105 82-83 1.0632100869222718 1105 84-85 1.3006681553266155 1105 86-87 1.4395050430217253 1105 88-89 1.558022412011237 1105 90-91 1.4873569740765298 1105 92-93 1.6134330862980342 1105 94-95 1.7604674983407627 1105 96-97 1.8303458156348071 1105 98-99 2.0591578190529205 1105 100-101 2.505456395445826 1106 1 -0.014461322277490751 1106 2 -0.03261528932299029 1106 3 0.03635014913260903 1106 4 0.03263794008243792 1106 5 0.010782673709847757 1106 6 -0.04069894140239683 1106 7 -0.036006207802863344 1106 8 -0.030660229540330874 1106 9 -0.01689496338330798 1106 10-11 -0.01349859853591795 1106 12-13 -0.049725500339782513 1106 14-15 -0.01760383100188534 1106 16-17 0.022862201552655392 1106 18-19 -0.039169439205196 1106 20-21 -0.06181275443841372 1106 22-23 -0.0268336438784047 1106 24-25 -0.023176942750957608 1106 26-27 -0.03382957902224604 1106 28-29 -0.06872582231076763 1106 30-31 0.04080441614794239 1106 32-33 0.059399397265543996 1106 34-35 -0.06999355153286047 1106 36-37 0.09473747037063163 1106 38-39 0.1398314654750763 1106 40-41 0.17854146344605937 1106 42-43 0.18127305259108795 1106 44-45 0.29927771306400786 1106 46-47 0.022569899351559286 1106 48-49 0.1932577491309857 1106 50-51 0.2324105558623586 1106 52-53 0.2572266242709844 1106 54-55 0.4444357276315145 1106 56-57 0.5848403399790953 1106 58-59 0.512742563451738 1106 60-61 0.4132558342324941 1106 62-63 0.3266995208205188 1106 64-65 0.4165752528881086 1106 66-67 0.46582247381740416 1106 68-69 0.17645245497177342 1106 70-71 0.4061231180481464 1106 72-73 0.5547312314469259 1106 74-75 0.6293062513971464 1106 76-77 0.5918178232227689 1106 78-79 0.8058516918952598 1106 80-81 1.046149459773865 1106 82-83 0.9183948742822174 1106 84-85 0.9767657953979345 1106 86-87 1.055689518817971 1106 88-89 1.210648124492618 1106 90-91 1.1708735620229795 1106 92-93 1.1999021380711241 1106 94-95 1.600763330532608 1106 96-97 1.7002818568433717 1106 98-99 2.1650949011214635 1106 100-101 2.512007550184215 1107 1 0.07060719353734157 1107 2 0.036972206966638055 1107 3 0.060228856942110554 1107 4 0.026119223184821294 1107 5 0.06932625915479207 1107 6 0.029351511503811878 1107 7 0.023387556547469046 1107 8 -0.027255577905343387 1107 9 0.049697498119428474 1107 10-11 0.021544068528513094 1107 12-13 0.03852582524847037 1107 14-15 0.09873149345659726 1107 16-17 0.06516464184027626 1107 18-19 0.061139592140541765 1107 20-21 0.10716668206290336 1107 22-23 0.0932169379661758 1107 24-25 0.06936599474474292 1107 26-27 0.11906988528632922 1107 28-29 0.05186392551428298 1107 30-31 0.130263012068788 1107 32-33 0.124576319034297 1107 34-35 0.1747854962803359 1107 36-37 0.16807761617414485 1107 38-39 0.1155237321994278 1107 40-41 0.22328905029657165 1107 42-43 0.24070733073504869 1107 44-45 0.1667243166304999 1107 46-47 0.29361681513892535 1107 48-49 0.29784373412717713 1107 50-51 0.3802924852310028 1107 52-53 0.33735320374906763 1107 54-55 0.4569177763563772 1107 56-57 0.42566476649854934 1107 58-59 0.5021159378951481 1107 60-61 0.7548359753345011 1107 62-63 0.7363798880261427 1107 64-65 0.5551948603969876 1107 66-67 0.7191264517290392 1107 68-69 0.7474259030322159 1107 70-71 0.8940756594981032 1107 72-73 0.7647431331372516 1107 74-75 0.8547273742983563 1107 76-77 0.8577264770369517 1107 78-79 0.9975819647655015 1107 80-81 1.0099565267095727 1107 82-83 1.1332828180151395 1107 84-85 1.1613961786258464 1107 86-87 1.4310899233587158 1107 88-89 1.4270191309488958 1107 90-91 1.4812704329737194 1107 92-93 1.678750951858465 1107 94-95 1.6661154048999158 1107 96-97 1.608828787899757 1107 98-99 1.5706301032649321 1107 100-101 1.9629163010414885 1108 1 0.07324005186338667 1108 2 0.08542518668372168 1108 3 0.16802991959016111 1108 4 0.058934905058279696 1108 5 0.045893565916081513 1108 6 0.07520721206141445 1108 7 0.08884343876213308 1108 8 0.041038632322297985 1108 9 0.02847338164543345 1108 10-11 0.08919899737398396 1108 12-13 0.05456415112669788 1108 14-15 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-0.8097470391927217 2316 52-53 -0.7440408922555619 2316 54-55 -0.7132238058152609 2316 56-57 -0.7754780457643164 2316 58-59 -0.9757292011427765 2316 60-61 -1.0539970641191232 2316 62-63 -1.0819731946134254 2316 64-65 -0.9323921696628048 2316 66-67 -0.9684199255859554 2316 68-69 -1.0951824323844583 2316 70-71 -1.1790704094091318 2316 72-73 -1.0721373135009031 2316 74-75 -1.1177467351576382 2316 76-77 -1.1127281036147778 2316 78-79 -1.2385532368610512 2316 80-81 -1.606889970738429 2316 82-83 -1.6290699194909166 2316 84-85 -1.650664467526024 2316 86-87 -1.5755032328208323 2316 88-89 -1.7639299087613658 2316 90-91 -1.9228764235458762 2316 92-93 -2.373268742875375 2316 94-95 -2.543457514509196 2316 96-97 -2.810078610125462 2316 98-99 -3.1596350632509242 2316 100-101 -3.617006386840565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 3.0 4 21.0 5 53.0 6 91.0 7 283.0 8 663.0 9 1223.0 10 2133.0 11 3494.0 12 5281.0 13 7789.0 14 10771.0 15 14907.0 16 19339.0 17 27618.0 18 27998.0 19 20839.0 20 18526.0 21 18093.0 22 18980.0 23 20330.0 24 23031.0 25 26642.0 26 31573.0 27 37926.0 28 45161.0 29 55376.0 30 67076.0 31 83073.0 32 104631.0 33 137350.0 34 191942.0 35 288071.0 36 431847.0 37 556007.0 38 311142.0 39 17521.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.844032817003445 18.338600179914106 14.984416453575422 22.832950549507025 2 16.163556374560013 19.685284000159907 39.24165094546154 24.909508679818543 3 19.803840797489723 23.46128631157196 29.53299307633092 27.201879814607395 4 13.356357466416476 16.590661901950238 35.42275056922677 34.63023006240652 5 13.896478408098082 37.0673767842561 33.62687229113806 15.40927251650776 6 33.19563743455448 35.475481031504835 16.365825682397958 14.96305585154273 7 29.466055583028687 32.8050117081219 19.334104611336418 18.394828097512992 8 27.608129416485266 33.84749387659385 18.763433614815863 19.780943092105012 9 26.77838396320402 16.47569351254697 19.566553944348446 37.179368579900554 10-11 25.651769885058258 26.274902362524266 25.917263112772947 22.156064639644526 12-13 26.734281023096845 23.46881710851455 26.81239861748608 22.98450325090252 14-15 23.17983530587873 24.832353627538204 25.37255278172109 26.615258284861977 16-17 22.44868630507325 28.09888882269111 26.49420389800018 22.958220974235466 18-19 22.65231312973269 25.872646241123736 29.239126255468133 22.235914373675435 20-21 22.87851914623408 25.049898945831234 29.884947858789886 22.1866340491448 22-23 22.846883804646627 24.890808581819236 29.88546179033192 22.37684582320222 24-25 22.656523626064047 25.313085321841356 29.66314014222845 22.367250909866147 26-27 21.846212484964287 25.77562201879717 29.586763925860744 22.791401570377808 28-29 22.2243942773728 26.148540781837067 29.403774668080047 22.223290272710088 30-31 22.151714050936814 25.58440324904394 29.361769923026216 22.902112776993025 32-33 21.84717173136159 25.644712612302907 29.369520004659815 23.138595651675686 34-35 22.79998560747589 25.77969500413785 29.074764403328025 22.34555498505824 36-37 22.94132562445247 25.20877901728521 29.068035594561902 22.781859763700414 38-39 22.307041624440476 25.623178593982598 29.027007689910285 23.042772091666638 40-41 22.76202777274299 25.371700711149153 29.15454509453303 22.711726421574824 42-43 22.399032552358992 25.52010591358463 28.912569508645646 23.16829202541073 44-45 22.40166516690218 25.757260500429563 29.195341294300242 22.645733038368018 46-47 23.154906445947233 26.167381778997456 28.146886058726327 22.530825716328984 48-49 22.768907357483997 26.286017424613167 27.948190984358735 22.996884233544105 50-51 22.994877054250995 26.523112262825354 27.97904225240587 22.50296843051779 52-53 23.390699514125313 26.03879840521332 27.96706955092662 22.603432529734746 54-55 22.646945400293664 26.455977575834407 28.09764623160649 22.799430792265444 56-57 22.726319271785652 26.70789920393908 27.784985509033966 22.780796015241307 58-59 22.665941832847384 26.115735860506035 27.80352511355032 23.414797193096263 60-61 22.224208155217983 26.509045766174854 28.19002066766179 23.07672541094537 62-63 22.44382823418071 26.496463960645787 28.34123074802926 22.718477057144238 64-65 22.111657147512435 26.54768580433522 28.10683989585844 23.233817152293902 66-67 22.046505643083915 26.625880658192603 28.014928432870494 23.312685265852988 68-69 22.693334094716004 26.143977463072943 28.283824425156084 22.878864017054973 70-71 22.370678644697804 26.47843828767147 27.72811041838215 23.422772649248575 72-73 22.396755911830667 26.55484277692059 27.61611141122175 23.43228990002699 74-75 22.393196460039537 27.07836770773969 27.3806736614653 23.147762170755467 76-77 22.543340608319827 26.535941516874654 27.784757095015284 23.13596077979023 78-79 22.5530101351107 26.79606701515032 27.693505694551828 22.957417155187148 80-81 22.785067695446344 26.80807818938736 27.417050736418414 22.98980337874788 82-83 22.543555711471026 26.94194545069352 27.523417934394566 22.991080903440885 84-85 22.38539420312973 27.108240516037075 27.530746155695496 22.975619125137698 86-87 22.764665439312196 27.040457775808324 27.11484520258201 23.080031582297465 88-89 22.5107387919599 27.24372560847127 26.839185269881682 23.406350329687147 90-91 22.332894700024116 27.227878728581413 27.065870565928336 23.373356005466135 92-93 22.43352970075493 26.665609611109513 27.385003807874504 23.515856880261047 94-95 22.17377301559384 26.469941061502066 27.539871615947277 23.816414306956812 96-97 22.66201538524206 25.983562528290093 27.51140835456637 23.84301373190148 98-99 22.12485412392219 26.54186089051647 27.066072560107795 24.26721242545354 100-101 22.4063598744974 25.975147317841635 27.407600014999705 24.210892792661262 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1635.0 1 1080.0 2 805.5 3 1072.5 4 827.0 5 757.5 6 1558.5 7 3464.5 8 5671.5 9 6498.5 10 5174.5 11 3535.5 12 3207.5 13 3364.5 14 3513.5 15 3664.5 16 3780.5 17 4005.5 18 4348.0 19 4753.0 20 5166.0 21 5870.5 22 7043.5 23 8466.0 24 10215.5 25 12395.5 26 15238.0 27 18757.5 28 21655.5 29 24420.5 30 28091.5 31 33699.5 32 42151.0 33 44312.5 34 41886.5 35 44089.0 36 48842.0 37 53774.5 38 58199.5 39 61898.0 40 65190.0 41 69819.5 42 72701.0 43 75377.5 44 79379.5 45 85179.0 46 88347.5 47 89834.0 48 91271.5 49 90711.0 50 92464.0 51 92739.5 52 93709.5 53 98771.5 54 104131.5 55 99835.5 56 90970.0 57 84648.5 58 79831.5 59 71642.0 60 57609.0 61 47994.0 62 41144.0 63 30499.0 64 21175.0 65 16076.0 66 11868.5 67 8996.5 68 7043.5 69 5317.5 70 4036.5 71 2877.5 72 2077.0 73 1734.5 74 1305.5 75 895.5 76 602.5 77 371.5 78 237.0 79 130.0 80 69.5 81 48.0 82 30.0 83 16.0 84 11.5 85 6.5 86 4.0 87 3.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011953666977690422 3 0.006357523519981849 4 0.0 5 5.329660435912926E-4 6 2.284140186819826E-4 7 0.0 8 1.142070093409913E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.9034501556831882E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018653811525695244 26-27 0.021394779749879037 28-29 3.23586526466142E-4 30-31 2.0937951712515071E-4 32-33 0.0036546242989117217 34-35 0.01695974088713721 36-37 0.008679732709915339 38-39 0.021908711291913498 40-41 0.024249954983403817 42-43 0.019681674609764166 44-45 0.010868700388951005 46-47 0.0024935197039449766 48-49 2.855175233524782E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.6165552957980574E-4 68-69 3.426210280229739E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.855175233524782E-4 82-83 0.001123035591853081 84-85 0.003749796806695881 86-87 7.994490653869392E-4 88-89 1.9034501556831882E-5 90-91 0.003407175778672907 92-93 5.900695482617884E-4 94-95 1.142070093409913E-4 96-97 2.284140186819826E-4 98-99 2.855175233524782E-4 100-101 0.0034452447817865705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2626809.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.52968246462604 #Duplication Level Percentage of deduplicated Percentage of total 1 71.77225696980072 32.6776806960457 2 14.88478067992181 13.553986758248811 3 5.428271044004193 7.4144237089630485 4 2.573908420069687 4.687569322352006 5 1.3788710462324558 3.1389780447315205 6 0.851769635161954 2.3268480613160483 7 0.5471874672685821 1.7439290143353048 8 0.40754705491207516 1.4844390399632237 9 0.2944149608593106 1.2064157712683745 >10 1.637090251520722 13.80207054387762 >50 0.13857211394892927 4.371667059898253 >100 0.07534751463841555 6.354690253098444 >500 0.0051172549692689185 1.5919122813625535 >1k 0.004613918414911126 4.316714271628687 >5k 8.388942572565684E-5 0.29719209820952946 >10k+ 1.677788514513137E-4 1.0314830747007522 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14611 0.5562262044937413 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12395 0.47186529359386237 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7781 0.2962149132274178 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4716 0.17953341868403833 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4376 0.16658995762539264 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4335 0.1650291284977324 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4264 0.1623262292766623 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4094 0.15585449874733945 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3606 0.13727682522787155 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3340 0.12715047039963698 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3267 0.12437143317233952 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3257 0.12399074314120288 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2908 0.11070466105453423 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2816 0.10720231276807717 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5227601245465505E-4 2 0.0 0.0 0.0 0.0 1.5227601245465505E-4 3 0.0 0.0 0.0 0.0 1.5227601245465505E-4 4 0.0 0.0 0.0 0.0 1.5227601245465505E-4 5 0.0 0.0 0.0 0.0 1.5227601245465505E-4 6 0.0 0.0 0.0 0.0 1.5227601245465505E-4 7 0.0 0.0 0.0 0.0 1.5227601245465505E-4 8 0.0 0.0 0.0 0.0 1.5227601245465505E-4 9 0.0 0.0 0.0 0.0 1.5227601245465505E-4 10-11 0.0 0.0 0.0 3.8069003113663764E-5 1.5227601245465505E-4 12-13 0.0 0.0 0.0 3.8069003113663764E-5 4.1875903425030143E-4 14-15 0.0 0.0 0.0 3.8069003113663764E-5 5.139315420344608E-4 16-17 0.0 0.0 0.0 7.613800622732753E-5 5.710350467049564E-4 18-19 0.0 0.0 0.0 1.3324151089782316E-4 5.900695482617883E-4 20-21 0.0 0.0 0.0 1.7131051401148695E-4 6.852420560459478E-4 22-23 0.0 0.0 0.0 2.2841401868198258E-4 7.233110591596115E-4 24-25 0.0 0.0 0.0 5.329660435912927E-4 7.233110591596115E-4 26-27 0.0 0.0 0.0 0.0011801390965235766 7.804145638301072E-4 28-29 0.0 0.0 0.0 0.004111452336275687 7.994490653869391E-4 30-31 0.0 0.0 0.0 0.012334357008827061 8.375180685006027E-4 32-33 0.0 0.0 0.0 0.028875338861713964 9.136560747279303E-4 34-35 0.0 0.0 0.0 0.054038949919845716 9.89794080955258E-4 36-37 0.0 0.0 0.0 0.0939162306814085 9.89794080955258E-4 38-39 0.0 0.0 0.0 0.1565968443080559 0.0010659320871825855 40-41 0.0 0.0 0.0 0.22504491190642334 0.0012182080996372404 42-43 0.0 0.0 0.0 0.2919131158755737 0.0012182080996372404 44-45 0.0 0.0 0.0 0.37393278308396233 0.0012562771027509042 46-47 0.0 0.0 0.0 0.4683439108058485 0.0013133806074214 48-49 0.0 0.0 0.0 0.5696074590881941 0.0013324151089782318 50-51 0.0 0.0 0.0 0.6807308791769786 0.001446622118319223 52-53 0.0 0.0 0.0 0.7786443551853217 0.0015608291276602143 54-55 0.0 0.0 0.0 0.8859418404611832 0.0015988981307738781 56-57 0.0 0.0 0.0 1.0100087216086133 0.0015988981307738781 58-59 0.0 0.0 0.0 1.142070093409913 0.00161793263233071 60-61 0.0 0.0 0.0 1.2870178227651876 0.0016750361370012057 62-63 0.0 0.0 0.0 1.4239139579619227 0.001751174143228533 64-65 0.0 0.0 0.0 1.5668249956506164 0.001751174143228533 66-67 0.0 0.0 0.0 1.7337004707993615 0.001770208644785365 68-69 0.0 0.0 0.0 1.9147756841095032 0.0017892431463421969 70-71 0.0 0.0 0.0 2.1004001432917274 0.0018463466510126926 72-73 0.0 0.0 0.0 2.2930864025515367 0.0020557261681378434 74-75 0.0 0.0 0.0 2.487599973960802 0.0021699331774788348 76-77 0.0 0.0 0.0 2.689898656506811 0.0022080021805924986 78-79 0.0 0.0 0.0 2.9211488159207617 0.00222703668214933 80-81 0.0 0.0 0.0 3.1667700240101206 0.002246071183706162 82-83 0.0 0.0 0.0 3.419871791211314 0.0023222091899334895 84-85 0.0 0.0 0.0 3.6739823869950197 0.0023412436914903214 86-87 0.0 0.0 0.0 3.933270367202183 0.0025315887070586404 88-89 0.0 0.0 0.0 4.2120687115051 0.0027028992210701274 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6210 0.0 60.353313 1 GTATCAA 11420 0.0 43.30124 1 GTGGTAT 2400 0.0 34.241325 1 TGGTATC 2340 0.0 34.1043 2 ATCAACG 14460 0.0 33.834507 3 TATCAAC 14605 0.0 33.760704 2 TCAACGC 14810 0.0 33.129234 4 CAACGCA 14995 0.0 32.78385 5 AACGCAG 15510 0.0 31.817778 6 ACGCAGA 17820 0.0 27.480022 7 CGCAGAG 17920 0.0 27.220655 8 TAAGGTA 865 0.0 26.356712 4 GTGTAAG 1145 0.0 24.892096 1 TAAGGTG 1925 0.0 24.673761 5 GCAGAGT 19530 0.0 24.611856 9 GTAAGGT 1995 0.0 24.046097 4 TGTAAGG 1085 0.0 23.203945 2 AGGTAAG 1830 0.0 22.842691 2 GAGTACT 12665 0.0 22.764053 12-13 CAGAGTA 18615 0.0 22.491861 10-11 >>END_MODULE