FastQCFastQC Report
Thu 26 May 2016
SRR938269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641275
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152470.5772590888869958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124000.4694702369120974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75540.2859982394866116No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47810.18101106473199496No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46300.17529412878250086No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA43080.16310304682397705No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC40890.1548115966720618No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39680.15023047581187116No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35490.1343669250645995No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA32930.12467463630254329No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32440.12281947165668095No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32270.12217584310607567No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30020.1136572299362997No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA26720.10116326395396164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63850.061.995361
GTATCAA115850.043.0298651
GTGGTAT24450.036.9103741
TGGTATC23250.036.376222
ATCAACG146850.033.6825943
TATCAAC147300.033.6758272
TCAACGC150050.032.9959374
CAACGCA152850.032.422585
AACGCAG157450.031.5658686
ACGCAGA179500.027.7548947
CGCAGAG180550.027.5671548
TAAGGTG20500.026.4228535
AGGTAAG18850.025.7104282
GTGTAAG11400.025.5499781
GTAAGGT20950.025.4016954
GCAGAGT194250.024.8148389
TAAGGTA9350.023.8844384
GAGTACT124450.023.75730112-13
GTACATG115000.023.7500081
TACATGG112800.023.7148192
Produced by FastQC (version 0.11.5)