FastQCFastQC Report
Thu 26 May 2016
SRR938269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641275
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148580.5625313532290277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125400.47477070732884685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78510.29724280887071586No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA46660.17665710688966502No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45300.17150807848482266No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC43830.1659425845472357No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA43140.16333020984183774No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA41120.15568238824052777No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG35070.13277678393957465No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34950.13232245790385327No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34130.12921789665975714No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA32770.12406886825491475No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30810.11664820967146548No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA27340.10351061513852212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59350.057.071441
GTATCAA113450.044.8052941
TGGTATC24700.037.120912
GTGGTAT26150.034.880251
ATCAACG148050.034.1697583
TCAACGC149650.033.802514
TATCAAC150150.033.6963272
CAACGCA152000.033.3111535
AACGCAG156050.032.477066
TAAGGTG17650.029.8713115
ACGCAGA177850.028.4160547
CGCAGAG179850.027.94168
AGGTAAG17000.026.5480692
GTAAGGT18950.025.5662484
GCAGAGT197800.025.237389
AAGGTAA18350.023.558921
GTACATG115400.022.7650891
GTGTAAG10550.022.5147721
GAGTACT126750.022.46513612-13
TACATGG115750.022.4093842
Produced by FastQC (version 0.11.5)