##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938269_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2641275 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50131811341114 34.0 31.0 34.0 31.0 34.0 2 32.71558130069758 34.0 31.0 34.0 31.0 34.0 3 32.767589895031755 34.0 31.0 34.0 31.0 34.0 4 36.23421112909485 37.0 35.0 37.0 35.0 37.0 5 36.196154508712645 37.0 35.0 37.0 35.0 37.0 6 36.23976185743628 37.0 37.0 37.0 35.0 37.0 7 36.20404085148271 37.0 37.0 37.0 35.0 37.0 8 36.19519285193704 37.0 37.0 37.0 35.0 37.0 9 37.9019447047354 39.0 38.0 39.0 35.0 39.0 10-11 37.890036819339144 39.0 38.0 39.0 35.0 39.0 12-13 37.83890961751427 39.0 38.0 39.0 35.0 39.0 14-15 39.1489195558963 40.5 38.5 41.0 36.0 41.0 16-17 39.098388467690796 40.0 38.0 41.0 36.0 41.0 18-19 39.13861033023824 40.0 39.0 41.0 36.0 41.0 20-21 39.06828084921108 40.0 39.0 41.0 35.5 41.0 22-23 38.926961789287375 40.0 38.0 41.0 35.0 41.0 24-25 38.87650471836518 40.0 38.0 41.0 35.0 41.0 26-27 38.828462768927885 40.0 38.0 41.0 35.0 41.0 28-29 38.76825150732128 40.0 38.0 41.0 35.0 41.0 30-31 38.55065470274772 40.0 38.0 41.0 34.5 41.0 32-33 38.35316068943976 40.0 38.0 41.0 34.0 41.0 34-35 38.14436096203538 40.0 38.0 41.0 34.0 41.0 36-37 37.99745653141002 40.0 37.5 41.0 33.5 41.0 38-39 37.96070117651513 40.0 37.5 41.0 33.0 41.0 40-41 37.87408013175455 40.0 37.5 41.0 33.0 41.0 42-43 37.70290143964563 40.0 37.0 41.0 33.0 41.0 44-45 37.47271526062224 40.0 37.0 41.0 33.0 41.0 46-47 37.11533123207542 40.0 36.5 41.0 31.5 41.0 48-49 36.73296476133685 40.0 36.0 41.0 31.0 41.0 50-51 36.405289869475915 39.5 35.5 41.0 30.0 41.0 52-53 35.96430511779349 39.0 35.0 41.0 29.0 41.0 54-55 35.71995589251404 39.0 35.0 40.5 28.5 41.0 56-57 35.45265127637221 38.5 35.0 40.0 28.0 41.0 58-59 35.089621678924004 38.0 34.5 40.0 27.5 41.0 60-61 34.70859755231848 37.5 34.0 40.0 26.5 41.0 62-63 34.32458149946522 37.0 34.0 40.0 26.0 41.0 64-65 33.9160965821431 36.0 34.0 39.0 26.0 41.0 66-67 33.488447246121666 36.0 33.0 39.0 25.0 40.5 68-69 33.03116487302533 35.0 33.0 38.5 23.5 40.0 70-71 32.50955069048092 35.0 32.0 37.5 22.5 40.0 72-73 32.10369651020814 35.0 32.0 37.0 21.5 39.0 74-75 31.670392518764615 35.0 31.5 36.5 20.0 39.0 76-77 30.3912572526526 33.5 30.0 35.0 19.5 37.5 78-79 31.124281644281645 34.5 31.0 36.0 20.0 37.5 80-81 30.980612204333134 35.0 31.0 35.0 18.5 37.0 82-83 30.630491523979895 34.0 31.0 35.0 17.5 37.0 84-85 30.27548399920493 34.0 31.0 35.0 13.5 36.0 86-87 29.86531826485315 34.0 30.5 35.0 7.0 36.0 88-89 29.405762368553066 34.0 30.0 35.0 4.5 35.5 90-91 29.07230958533284 34.0 29.0 35.0 2.0 35.0 92-93 28.673211611816264 34.0 29.0 35.0 2.0 35.0 94-95 28.26262183983114 34.0 29.0 35.0 2.0 35.0 96-97 27.688135275577135 33.5 28.0 35.0 2.0 35.0 98-99 26.990089445438283 33.0 26.5 35.0 2.0 35.0 100-101 24.522985489962235 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11752198198544761 1101 2 0.10837279190983651 1101 3 0.11466478327910323 1101 4 0.10042153932684528 1101 5 0.04493252673381676 1101 6 0.06396153822192474 1101 7 0.057172578706783383 1101 8 0.05762462859889439 1101 9 0.035262299443282075 1101 10-11 0.09799873339400023 1101 12-13 0.09837861081415866 1101 14-15 0.11347953246978904 1101 16-17 0.11001840886069658 1101 18-19 0.08580510296945931 1101 20-21 0.1422890319771497 1101 22-23 0.21864610926457573 1101 24-25 0.16885296080126722 1101 26-27 0.09553654807098155 1101 28-29 0.050113999793289565 1101 30-31 -0.07241887206480158 1101 32-33 0.009033516350029913 1101 34-35 -0.09709692411213666 1101 36-37 -0.06347773387219746 1101 38-39 0.01464241135672495 1101 40-41 0.02219669809301905 1101 42-43 -0.0017086168414053304 1101 44-45 0.044794399054381984 1101 46-47 0.07526470106022742 1101 48-49 0.08072068275929212 1101 50-51 0.017015293191981584 1101 52-53 -0.08296469643171633 1101 54-55 -0.025467540563830937 1101 56-57 -0.04756903470589435 1101 58-59 -0.08703534667065327 1101 60-61 0.042360631871176224 1101 62-63 -0.18367700565143252 1101 64-65 -0.08979964509466498 1101 66-67 -0.1038008670608761 1101 68-69 -0.14570498576206603 1101 70-71 -0.08449239308190926 1101 72-73 -0.11861444956977607 1101 74-75 -0.0778744466564909 1101 76-77 -0.13315758616823103 1101 78-79 -0.18171465299042922 1101 80-81 0.07676970689169949 1101 82-83 0.1065207583813681 1101 84-85 0.19353807536015566 1101 86-87 0.2560504392982317 1101 88-89 0.25113311989064613 1101 90-91 0.21674387556692665 1101 92-93 0.25534640344994486 1101 94-95 0.21858135284508862 1101 96-97 0.3245790552958141 1101 98-99 0.40695654449467256 1101 100-101 0.5320542009704354 1102 1 0.09810778337917725 1102 2 0.15837279190984077 1102 3 0.09188603763450232 1102 4 0.07583530239305247 1102 5 0.11437939084531479 1102 6 0.07961920372715525 1102 7 0.03257545327124234 1102 8 0.08371347877311308 1102 9 0.1125488848091365 1102 10-11 0.09278314105950614 1102 12-13 0.14460574496050072 1102 14-15 0.19230139658128564 1102 16-17 0.2344794280244571 1102 18-19 0.15414133990325496 1102 20-21 0.07775832640223967 1102 22-23 0.1585851336548174 1102 24-25 0.0691251733448226 1102 26-27 0.04459469267028737 1102 28-29 0.037107684462277746 1102 30-31 0.02067237357979934 1102 32-33 -0.021650281559381312 1102 34-35 0.18261779714222115 1102 36-37 0.1455760553264085 1102 38-39 -0.008593651361046284 1102 40-41 0.11048611446933165 1102 42-43 0.0911757472700927 1102 44-45 0.24556203842719526 1102 46-47 0.04980193973618441 1102 48-49 -0.02928040609088356 1102 50-51 -0.048239497748781446 1102 52-53 -0.07512497517735994 1102 54-55 0.00179726779853695 1102 56-57 0.13498214125228714 1102 58-59 0.12921574217952525 1102 60-61 0.1900686022544491 1102 62-63 0.036357837554142236 1102 64-65 0.024486069191056004 1102 66-67 0.004588723531462335 1102 68-69 0.1723808156316622 1102 70-71 0.15555007207488103 1102 72-73 0.06306020199816942 1102 74-75 0.07046505682783177 1102 76-77 0.15415730930214977 1102 78-79 0.08604993760190638 1102 80-81 0.3189680953934442 1102 82-83 0.21455102841621354 1102 84-85 -0.014535748681655747 1102 86-87 0.0502468678696637 1102 88-89 -0.04237188882015275 1102 90-91 0.07301565257041176 1102 92-93 0.06857593306666843 1102 94-95 0.009244462601184722 1102 96-97 0.04302417724703034 1102 98-99 0.0020676072124423683 1102 100-101 0.15788172710283987 1103 1 0.15172073551038068 1103 2 0.10087894178683854 1103 3 0.17865125354969535 1103 4 0.05465407967604108 1103 5 0.09044499148452445 1103 6 0.05411386017548381 1103 7 0.07902732911177424 1103 8 0.03577681555515966 1103 9 0.04709293783052004 1103 10-11 0.04457748931887551 1103 12-13 0.094759089454584 1103 14-15 0.17465187152300388 1103 16-17 0.20941776462358064 1103 18-19 0.22965147604199387 1103 20-21 0.1954598748102896 1103 22-23 0.23610651005655825 1103 24-25 0.18617030195444073 1103 26-27 0.16100864487904687 1103 28-29 0.17411567600976952 1103 30-31 0.22045767665422034 1103 32-33 0.22122714747740702 1103 34-35 0.0638590442684972 1103 36-37 0.22169834385623943 1103 38-39 0.22636805184391307 1103 40-41 0.28945732163055027 1103 42-43 0.14126111751391335 1103 44-45 0.15414005589124002 1103 46-47 0.1990031325415984 1103 48-49 0.3342882676575982 1103 50-51 0.2830700345971593 1103 52-53 0.21242814571144208 1103 54-55 0.24963520738121048 1103 56-57 0.39401003996260187 1103 58-59 0.35374405649128704 1103 60-61 0.34950864516090974 1103 62-63 0.43825705566734285 1103 64-65 0.3892637736369622 1103 66-67 0.4254906408589605 1103 68-69 0.4957858719207735 1103 70-71 0.5336856496072357 1103 72-73 0.363971836204513 1103 74-75 0.356118154741484 1103 76-77 0.29802135275298625 1103 78-79 0.3236367712310866 1103 80-81 0.5041586903620328 1103 82-83 0.5338831173841889 1103 84-85 0.7119690504906551 1103 86-87 0.7329582136427497 1103 88-89 0.5379151030009837 1103 90-91 0.5018769229059821 1103 92-93 0.6261815492470291 1103 94-95 0.41076960283009 1103 96-97 0.5050820702355132 1103 98-99 0.49356012492306434 1103 100-101 0.6806667287198813 1104 1 0.11168960871356148 1104 2 0.028418874859148957 1104 3 0.05999888466158865 1104 4 0.007938820432840998 1104 5 0.06615948525916338 1104 6 -0.0160211806720838 1104 7 0.06430103492797912 1104 8 -0.0337924220923469 1104 9 0.04231875105618599 1104 10-11 0.021169816343302728 1104 12-13 0.05207244721968607 1104 14-15 0.1730849472163314 1104 16-17 0.04399738351507665 1104 18-19 0.05075325965148636 1104 20-21 0.11182388220756678 1104 22-23 0.194279749817575 1104 24-25 0.10224869812845583 1104 26-27 0.03379259645807764 1104 28-29 0.0819760389637949 1104 30-31 0.13861943438681124 1104 32-33 0.22997245644219078 1104 34-35 0.3868460482380982 1104 36-37 0.27987624078217266 1104 38-39 0.26887628232446303 1104 40-41 0.42296714740177777 1104 42-43 0.4628579154166559 1104 44-45 0.4095049405290325 1104 46-47 0.5079619936869548 1104 48-49 0.5993338740035341 1104 50-51 0.6641207079385296 1104 52-53 0.49356180126414273 1104 54-55 0.5197801552887 1104 56-57 0.6923287187503249 1104 58-59 0.6738445496427161 1104 60-61 0.6103689844057385 1104 62-63 0.6209600911227611 1104 64-65 0.652740677485987 1104 66-67 0.6225456191142413 1104 68-69 0.6184223183853987 1104 70-71 0.5230320884849107 1104 72-73 0.517719075775851 1104 74-75 0.4524610948181653 1104 76-77 0.35913792074421025 1104 78-79 0.5874457732768512 1104 80-81 0.5395361239423941 1104 82-83 0.5522652860311403 1104 84-85 0.5004721191389585 1104 86-87 0.5000740568097548 1104 88-89 0.4350832927017052 1104 90-91 0.5479650737236099 1104 92-93 0.7400382237264402 1104 94-95 0.5728368251953171 1104 96-97 0.5335076267243828 1104 98-99 0.6699605767527395 1104 100-101 0.7492489014312653 1105 1 0.11946342716817071 1105 2 0.12016681184339006 1105 3 0.10094662159582413 1105 4 0.05542569215077009 1105 5 0.13511109816239042 1105 6 0.15869437321639168 1105 7 0.06687980462040599 1105 8 0.08255541486688855 1105 9 0.07862470254406162 1105 10-11 0.13680341224227988 1105 12-13 0.13131569553176092 1105 14-15 0.18468315926934764 1105 16-17 0.13584378261485597 1105 18-19 0.15049225690079737 1105 20-21 0.19211426730383607 1105 22-23 0.18914721668428314 1105 24-25 0.107740696127955 1105 26-27 0.11739943566788469 1105 28-29 0.05435507122186323 1105 30-31 0.13839750390418715 1105 32-33 0.1329607035039615 1105 34-35 0.2918738676929564 1105 36-37 0.23732341507574972 1105 38-39 0.2229272950776533 1105 40-41 0.1561269998648882 1105 42-43 0.2142191932361115 1105 44-45 0.22330526561029984 1105 46-47 0.09613091091626558 1105 48-49 0.21104148840713322 1105 50-51 0.21554450138689418 1105 52-53 0.06677229138666974 1105 54-55 0.17421407626894592 1105 56-57 0.1722174409308721 1105 58-59 0.19181466717209616 1105 60-61 0.23489073984459452 1105 62-63 0.18395865326330352 1105 64-65 0.3100065564557255 1105 66-67 0.20996747457810727 1105 68-69 0.23734111058344354 1105 70-71 0.1974756993876312 1105 72-73 0.17175445962180902 1105 74-75 0.22813157493819247 1105 76-77 0.2373262178184774 1105 78-79 0.2577492866551978 1105 80-81 0.20335989238450125 1105 82-83 0.42766382316542106 1105 84-85 0.4218274579736665 1105 86-87 0.3545658047467377 1105 88-89 0.289839169170822 1105 90-91 0.3311264890774481 1105 92-93 0.3897006393303464 1105 94-95 0.20325224154940358 1105 96-97 0.555939797267019 1105 98-99 0.6064962731768446 1105 100-101 0.776544787902882 1106 1 -0.06852563932629607 1106 2 -0.0026471504908585075 1106 3 0.008766967705554407 1106 4 -0.03655375002814765 1106 5 -0.03269034725411757 1106 6 -0.04459039624528316 1106 7 -0.01262429544416932 1106 8 -0.12909696570638118 1106 9 -0.1925738549880549 1106 10-11 -0.055215246663600226 1106 12-13 -0.10841180718833954 1106 14-15 -0.2578846812453648 1106 16-17 -0.10297259464416442 1106 18-19 -0.00755535456464429 1106 20-21 0.02726218513152645 1106 22-23 0.05916735580046861 1106 24-25 0.060224102734643736 1106 26-27 0.009240071273715955 1106 28-29 -0.013978896066085156 1106 30-31 0.09843565912576224 1106 32-33 0.16062191226374267 1106 34-35 0.14931008153911307 1106 36-37 0.05683646422050259 1106 38-39 0.1021542980056438 1106 40-41 0.13280476810877673 1106 42-43 0.07344293793227763 1106 44-45 0.1628621903112375 1106 46-47 0.21825828566599625 1106 48-49 0.15379428149319807 1106 50-51 0.14162863748367727 1106 52-53 0.02953394509412277 1106 54-55 -0.2586774791608022 1106 56-57 -0.17240949889652057 1106 58-59 -0.08981436028255985 1106 60-61 -0.00964184394798906 1106 62-63 0.030824706026010062 1106 64-65 -0.2615950045469262 1106 66-67 -0.19232235472592407 1106 68-69 -0.16908143932941755 1106 70-71 -0.29793933447733423 1106 72-73 -0.11540080913071193 1106 74-75 -0.07653949107859503 1106 76-77 0.01827883044862233 1106 78-79 -0.0037216796980317213 1106 80-81 -0.13151129688485597 1106 82-83 -0.3002329808187625 1106 84-85 0.06075138636922972 1106 86-87 0.28736853281559505 1106 88-89 0.13184728673844504 1106 90-91 0.04654404401772183 1106 92-93 0.13102614995866801 1106 94-95 0.008123224414941177 1106 96-97 0.003598440722104357 1106 98-99 0.16621030067572917 1106 100-101 0.10841810902890359 1107 1 0.12108196367042723 1107 2 0.15562553916258537 1107 3 0.07322705434137333 1107 4 0.05566421331952398 1107 5 0.05653966959096124 1107 6 0.05555952356991156 1107 7 0.06764647980568128 1107 8 0.03523451870878347 1107 9 -0.007068286637299082 1107 10-11 0.026696077716344746 1107 12-13 0.0623724294954755 1107 14-15 0.17402211488737152 1107 16-17 0.0923157989705885 1107 18-19 0.1197336743980344 1107 20-21 0.05014731952293516 1107 22-23 0.04039519351366039 1107 24-25 0.07300818058148906 1107 26-27 -0.014828607606673927 1107 28-29 0.10691917378596116 1107 30-31 0.06336981841138822 1107 32-33 0.15516446873098033 1107 34-35 0.1694209330307217 1107 36-37 0.12146388700691801 1107 38-39 0.1765242794885893 1107 40-41 0.10259390505272847 1107 42-43 0.08045141063112737 1107 44-45 0.08872182579430898 1107 46-47 0.11077866626168742 1107 48-49 0.17955882378493726 1107 50-51 0.29596446901616247 1107 52-53 0.336586585619564 1107 54-55 0.34637311877683175 1107 56-57 0.3320841246347612 1107 58-59 0.3805871350143306 1107 60-61 0.26967862637667395 1107 62-63 0.37154277456835416 1107 64-65 0.3239366186416035 1107 66-67 0.23284633989883474 1107 68-69 0.3335418091097253 1107 70-71 0.3963512424492208 1107 72-73 0.3198356420053159 1107 74-75 0.2685140606695171 1107 76-77 0.20430807317242738 1107 78-79 0.3769907041524263 1107 80-81 0.5290648076243052 1107 82-83 0.4583350898831995 1107 84-85 0.5652127548473374 1107 86-87 0.5506131682359658 1107 88-89 0.657675015495041 1107 90-91 0.5306175019405543 1107 92-93 0.661859681838223 1107 94-95 0.627389731965966 1107 96-97 0.7252429747097331 1107 98-99 0.8438269657400959 1107 100-101 0.7755523694686026 1108 1 0.01604523434747307 1108 2 0.05337253376538342 1108 3 -0.030025830588421343 1108 4 -0.010904226071467349 1108 5 -0.09381915120516027 1108 6 0.044495187351976995 1108 7 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-0.5112793094915951 2316 100-101 -0.43384926701574145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 4.0 4 12.0 5 39.0 6 93.0 7 247.0 8 619.0 9 1131.0 10 1839.0 11 3275.0 12 4832.0 13 7075.0 14 9453.0 15 13592.0 16 17673.0 17 25300.0 18 24904.0 19 17850.0 20 15142.0 21 14432.0 22 14618.0 23 15353.0 24 17754.0 25 20321.0 26 24171.0 27 29275.0 28 36072.0 29 43833.0 30 53842.0 31 66359.0 32 82822.0 33 109823.0 34 155754.0 35 241333.0 36 404435.0 37 631332.0 38 487672.0 39 48988.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83985007240821 18.40910166491562 14.971367994623808 22.77968026805236 2 16.233338142414695 19.711288504364337 39.18019173466594 24.875181618555025 3 19.59362882575065 23.420089410291155 29.634812688582674 27.35146907537552 4 13.355140983047962 16.546819244493662 35.47850943199781 34.61953034046058 5 13.86364539852912 37.099090401415985 33.62633576587065 15.410928434184248 6 33.186661744801285 35.421075048982026 16.362325013487805 15.02993819272889 7 29.475802406034962 32.87374468769818 19.31033307777494 18.340119828491922 8 27.62659379243945 33.85721149313794 18.736377255880992 19.779817458541615 9 26.77800220727568 16.452078833437767 19.58285139885623 37.18706756043033 10-11 25.702530028111426 26.238388656993305 25.929011556918535 22.130069757976734 12-13 26.731994964553103 23.52057245080501 26.727281331932495 23.020151252709393 14-15 23.20396399466167 24.839178048480374 25.366802017964808 26.590055938893148 16-17 22.430937092939587 28.136753935938756 26.43971014695774 22.99259882416391 18-19 22.641072966654363 25.863380375008283 29.242089521159286 22.253457137178067 20-21 22.88926749391866 25.040444482304945 29.874019176344756 22.19626884743164 22-23 22.845046426441776 24.88782879480554 29.86754503033573 22.399579748416958 24-25 22.626609776811186 25.302721148964057 29.70343720691962 22.367231867305133 26-27 21.897048003038602 25.70304621486757 29.61282423854096 22.78708154355287 28-29 22.203148934945787 26.1350204484654 29.376713661664787 22.285116954924025 30-31 22.11951086492525 25.583406798498228 29.367848741204295 22.929233595372235 32-33 21.840361629954756 25.619935307476894 29.32528314573688 23.21441991683147 34-35 22.78754623489171 25.781796872263417 29.086340790558662 22.344316102286207 36-37 22.953329434796014 25.232762176792296 29.053902989271958 22.760005399139736 38-39 22.324573137608745 25.636073670695637 29.019637023779026 23.019716167916595 40-41 22.77410101908959 25.379070086667582 29.155201711578236 22.69162718266459 42-43 22.38324108079823 25.518060286717738 28.93428490670021 23.164413725783827 44-45 22.428089178051692 25.758503370452996 29.194135366357866 22.619272085137446 46-47 23.1495715987004 26.19656767843239 28.125258516393703 22.528602206473504 48-49 22.752685729429917 26.26920710641641 27.93834417090231 23.039762993251365 50-51 22.935400516795866 26.574911737702433 27.947336040359293 22.5423517051424 52-53 23.432830735156315 26.023246348827744 27.961590519730056 22.582332396285885 54-55 22.67344369669951 26.446697144371562 28.062659132426575 22.817200026502352 56-57 22.76489574163993 26.692828274223622 27.76136524973734 22.780910734399107 58-59 22.73061305619445 26.08918041476181 27.76954311838033 23.41066341066341 60-61 22.264663846059197 26.483232529744154 28.205563600912438 23.04654002328421 62-63 22.436418017813367 26.491486119393098 28.36707650661139 22.705019356182145 64-65 22.114509091253275 26.584111839925797 28.120320678460214 23.181058390360715 66-67 22.05741545276429 26.61701261701262 28.022053742983978 23.303518187239117 68-69 22.705954622165304 26.130143955506213 28.293120632423634 22.870780789904845 70-71 22.42617675175815 26.446148167078398 27.73764564462239 23.390029436541067 72-73 22.363858363858363 26.59999432092455 27.619899480364595 23.416247834852484 74-75 22.39532044183207 27.053941751616172 27.448069587604472 23.10266821894729 76-77 22.53830063132389 26.512593349802653 27.8308960634542 23.118209955419257 78-79 22.506081343290646 26.806939830195642 27.738175691664065 22.948803134849648 80-81 22.75709647802671 26.83781507037321 27.40634731332406 22.99874113827602 82-83 22.581156889121267 26.95518411008631 27.506509171903094 22.957149828889328 84-85 22.38523704142617 27.131233660393644 27.480714295179666 23.002815003000524 86-87 22.72583316469599 27.08091696057521 27.119307590535534 23.073942284193265 88-89 22.45563647398254 27.337559954484096 26.84703918506009 23.359764386473273 90-91 22.346274803706635 27.225833393752648 27.09975736991027 23.328134432630442 92-93 22.438677642457648 26.728341601144145 27.38244043816084 23.450540318237362 94-95 22.13588134518367 26.503961155123946 27.613311752846638 23.746845746845747 96-97 22.651276372206606 26.05391335623894 27.526876224550644 23.767934047003813 98-99 22.118976630604536 26.645862320280926 27.08996602019858 24.145195028915957 100-101 22.191346982044656 26.24128498547103 27.577590368288043 23.989777664196268 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1535.0 1 1027.0 2 799.5 3 1089.5 4 826.0 5 712.0 6 1528.5 7 3518.5 8 5691.0 9 6488.0 10 5257.0 11 3603.5 12 3183.0 13 3371.0 14 3553.5 15 3625.5 16 3800.5 17 4077.0 18 4347.0 19 4672.0 20 5137.0 21 5960.5 22 7058.0 23 8422.0 24 10102.0 25 12282.5 26 15204.5 27 18633.5 28 21622.5 29 24615.0 30 28142.0 31 33288.5 32 42196.0 33 44955.5 34 42332.5 35 44150.5 36 49228.0 37 54460.5 38 58751.0 39 62113.0 40 65404.0 41 70713.5 42 73519.5 43 75815.5 44 79783.5 45 86252.0 46 89198.0 47 90515.0 48 91877.0 49 90960.0 50 93069.0 51 93669.0 52 95318.0 53 100362.5 54 105381.5 55 100575.0 56 91348.5 57 84811.5 58 80138.5 59 72064.5 60 57424.5 61 47907.0 62 41181.0 63 30136.0 64 20864.0 65 15983.0 66 11652.0 67 8830.5 68 6900.5 69 5144.0 70 3908.5 71 2767.5 72 1971.0 73 1662.5 74 1250.0 75 850.5 76 587.0 77 352.0 78 222.0 79 129.0 80 71.0 81 45.5 82 22.5 83 15.0 84 12.0 85 5.5 86 4.0 87 4.0 88 2.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016582900303830535 3 0.007269216571542153 4 0.0 5 0.0 6 0.0 7 0.0 8 4.543260357213846E-4 9 3.7860502976782044E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.0823276637230127E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005603354440563743 26-27 0.022261975750347846 28-29 3.786050297678205E-5 30-31 1.8930251488391025E-5 32-33 4.353957842329936E-4 34-35 0.02072862537978817 36-37 0.0042971670878647625 38-39 0.019819973308345402 40-41 0.016563970052342145 42-43 0.009294753480799991 44-45 0.0032938637589800383 46-47 5.679075446517307E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.8395377232586535E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.57210059535641E-5 84-85 0.002650235208374743 86-87 2.0823276637230127E-4 88-89 1.8930251488391025E-5 90-91 4.353957842329936E-4 92-93 2.8395377232586535E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2641275.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.409983727568886 #Duplication Level Percentage of deduplicated Percentage of total 1 71.93519075732871 32.665758417298605 2 14.762400266058338 13.407207117231353 3 5.347239446083153 7.284541687021513 4 2.538729732428579 4.611347033531083 5 1.4046458744781496 3.189247315152477 6 0.86839308551284 2.366022952936284 7 0.5596847448513823 1.7790692609388865 8 0.3982053548896859 1.4465998948617143 9 0.30313028526828417 1.2388627189229486 >10 1.651936497148517 13.797235718638564 >50 0.1441130094701686 4.518446896760879 >100 0.07634355599902892 6.423427292017404 >500 0.004827238733561213 1.4750595519655971 >1k 0.004910466987584394 4.464975671144877 >5k 8.322825402685414E-5 0.29672019632773344 >10k+ 1.6645650805370828E-4 1.0354782752499352 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14858 0.5625313532290277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12540 0.47477070732884685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7851 0.29724280887071586 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4666 0.17665710688966502 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4530 0.17150807848482266 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4383 0.1659425845472357 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4314 0.16333020984183774 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4112 0.15568238824052777 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3507 0.13277678393957465 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3495 0.13232245790385327 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3413 0.12921789665975714 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3277 0.12406886825491475 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3081 0.11664820967146548 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2734 0.10351061513852212 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5144201190712817E-4 2 0.0 0.0 0.0 0.0 1.5144201190712817E-4 3 0.0 0.0 0.0 0.0 1.5144201190712817E-4 4 0.0 0.0 0.0 0.0 1.5144201190712817E-4 5 0.0 0.0 0.0 0.0 1.5144201190712817E-4 6 0.0 0.0 0.0 0.0 1.5144201190712817E-4 7 0.0 0.0 0.0 3.7860502976782044E-5 1.5144201190712817E-4 8 0.0 0.0 0.0 3.7860502976782044E-5 1.5144201190712817E-4 9 0.0 0.0 0.0 3.7860502976782044E-5 1.5144201190712817E-4 10-11 0.0 0.0 0.0 3.7860502976782044E-5 1.5144201190712817E-4 12-13 0.0 0.0 0.0 3.7860502976782044E-5 5.111167901865576E-4 14-15 0.0 0.0 0.0 5.6790754465173065E-5 6.246982991169037E-4 16-17 0.0 0.0 0.0 9.465125744195511E-5 6.625588020936858E-4 18-19 0.0 0.0 0.0 1.5144201190712817E-4 7.193495565588589E-4 20-21 0.0 0.0 0.0 2.2716301786069226E-4 7.76140311024032E-4 22-23 0.0 0.0 0.0 3.975352812562115E-4 7.95070562512423E-4 24-25 0.0 0.0 0.0 7.76140311024032E-4 7.95070562512423E-4 26-27 0.0 0.0 0.0 0.0013440478556757626 9.654428259079422E-4 28-29 0.0 0.0 0.0 0.003956422561073724 0.0010222335803731152 30-31 0.0 0.0 0.0 0.012456105479361294 0.0010222335803731152 32-33 0.0 0.0 0.0 0.030704867914170243 0.0010600940833498972 34-35 0.0 0.0 0.0 0.05677182421368468 0.0011358150893034614 36-37 0.0 0.0 0.0 0.09629818932144513 0.0011358150893034614 38-39 0.0 0.0 0.0 0.15714001760513388 0.0012304663467454166 40-41 0.0 0.0 0.0 0.22131357015077946 0.0012493965982338076 42-43 0.0 0.0 0.0 0.28840238142563723 0.0013251176041873716 44-45 0.0 0.0 0.0 0.36913990402362495 0.0014197688616293268 46-47 0.0 0.0 0.0 0.46320432366944 0.001514420119071282 48-49 0.0 0.0 0.0 0.5612062356248403 0.001628001628001628 50-51 0.0 0.0 0.0 0.671380299287276 0.0016658621309784101 52-53 0.0 0.0 0.0 0.7724299817323073 0.001779443639908756 54-55 0.0 0.0 0.0 0.8818089748322306 0.0018173041428855383 56-57 0.0 0.0 0.0 1.0048934700097492 0.0018173041428855383 58-59 0.0 0.0 0.0 1.1370834161531835 0.0018173041428855383 60-61 0.0 0.0 0.0 1.2789088603042091 0.0018362343943739293 62-63 0.0 0.0 0.0 1.4170618356664868 0.0018930251488391023 64-65 0.0 0.0 0.0 1.5578839764886276 0.0019308856518158843 66-67 0.0 0.0 0.0 1.723561537515026 0.0019876764062810573 68-69 0.0 0.0 0.0 1.9064088366413947 0.0020066066577694485 70-71 0.0 0.0 0.0 2.0987969825179125 0.0020255369092578397 72-73 0.0 0.0 0.0 2.295652667745691 0.0022148394241417496 74-75 0.0 0.0 0.0 2.49332235378747 0.002366281436048878 76-77 0.0 0.0 0.0 2.698677721933536 0.0024041419390256596 78-79 0.0 0.0 0.0 2.9315766059952106 0.002460932693490833 80-81 0.0 0.0 0.0 3.1754739661716407 0.0024798629449792244 82-83 0.0 0.0 0.0 3.427132729458311 0.0027259562143283076 84-85 0.0 0.0 0.0 3.686155360573965 0.0027638167173050896 86-87 0.0 0.0 0.0 3.9536966048593953 0.0028206074717702628 88-89 0.0 0.0 0.0 4.23801005196354 0.0028773982262354355 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5935 0.0 57.07144 1 GTATCAA 11345 0.0 44.805294 1 TGGTATC 2470 0.0 37.12091 2 GTGGTAT 2615 0.0 34.88025 1 ATCAACG 14805 0.0 34.169758 3 TCAACGC 14965 0.0 33.80251 4 TATCAAC 15015 0.0 33.696327 2 CAACGCA 15200 0.0 33.311153 5 AACGCAG 15605 0.0 32.47706 6 TAAGGTG 1765 0.0 29.871311 5 ACGCAGA 17785 0.0 28.416054 7 CGCAGAG 17985 0.0 27.9416 8 AGGTAAG 1700 0.0 26.548069 2 GTAAGGT 1895 0.0 25.566248 4 GCAGAGT 19780 0.0 25.23738 9 AAGGTAA 1835 0.0 23.55892 1 GTACATG 11540 0.0 22.765089 1 GTGTAAG 1055 0.0 22.514772 1 GAGTACT 12675 0.0 22.465136 12-13 TACATGG 11575 0.0 22.409384 2 >>END_MODULE