FastQCFastQC Report
Thu 26 May 2016
SRR938268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641483
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146140.5532498221642917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117810.4459994631803422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72820.27567847303957665No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA48860.1849718510397379No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45600.17263029896463464No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA43260.1637716388861863No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40430.15305796024430218No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC39790.15063507885532484No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA36560.13840709934532988No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA33550.12701198531279587No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32030.12125764201397474No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31710.12004620131948605No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30920.11705545710496718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.061.1871221
GTATCAA113350.047.7600671
TGGTATC21550.037.7352332
ATCAACG146250.036.9386183
TATCAAC147800.036.551242
GTGGTAT23550.036.392141
TCAACGC148800.036.2736364
CAACGCA151750.035.5684855
AACGCAG156800.034.4486966
ACGCAGA178000.030.2343797
CGCAGAG179300.029.9587238
AGGTAAG17350.027.6834432
GCAGAGT193500.027.145019
TAAGGTG19650.026.3792345
TATACCG1457.814433E-726.2373625
TAAGGTA8350.025.6285614
GTAAGGT20450.024.649654
GTACATG113400.024.3523521
GGTAAGG20500.024.1255783
TACATGG114450.023.9334282
Produced by FastQC (version 0.11.5)