##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938268_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2641483 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96570108533729 33.0 31.0 34.0 30.0 34.0 2 32.07647749389263 33.0 31.0 34.0 30.0 34.0 3 32.0920263352064 34.0 31.0 34.0 30.0 34.0 4 35.62107308659567 37.0 35.0 37.0 33.0 37.0 5 35.623508082391595 37.0 35.0 37.0 33.0 37.0 6 35.66257060901017 37.0 35.0 37.0 35.0 37.0 7 35.59717704032167 37.0 35.0 37.0 33.0 37.0 8 35.587439328589284 37.0 35.0 37.0 33.0 37.0 9 37.21613843435676 39.0 37.0 39.0 34.0 39.0 10-11 37.127358949499204 39.0 37.0 39.0 34.0 39.0 12-13 36.97732523737613 39.0 37.0 39.0 33.0 39.0 14-15 38.14996954362379 40.0 38.0 41.0 33.0 41.0 16-17 37.986797567881375 40.0 38.0 41.0 33.0 41.0 18-19 38.15722853412269 40.0 38.0 41.0 33.0 41.0 20-21 38.15563170385727 40.0 38.0 41.0 33.0 41.0 22-23 38.04570803597827 40.0 38.0 41.0 33.0 41.0 24-25 37.91957207371768 40.0 38.0 41.0 33.0 41.0 26-27 37.73140239781971 40.0 38.0 41.0 32.5 41.0 28-29 37.55302816637472 40.0 37.5 41.0 32.0 41.0 30-31 37.30802091098069 40.0 37.0 41.0 31.5 41.0 32-33 36.99384474554635 40.0 36.0 41.0 31.0 41.0 34-35 36.74997756941839 39.0 36.0 41.0 30.0 41.0 36-37 36.47154174378559 39.0 35.0 41.0 30.0 41.0 38-39 36.154430673981246 39.0 35.0 40.5 29.5 41.0 40-41 36.0212102443968 39.0 35.0 41.0 29.0 41.0 42-43 35.78360678452218 39.0 35.0 40.5 27.5 41.0 44-45 35.43305162289517 39.0 35.0 40.0 26.5 41.0 46-47 35.0178085567842 38.0 34.5 40.0 25.0 41.0 48-49 34.57433892249165 38.0 34.0 40.0 23.5 41.0 50-51 33.80738774393021 37.5 33.5 39.5 22.5 40.5 52-53 33.85681054922557 38.0 33.5 39.5 23.0 40.5 54-55 33.929111033461126 38.0 33.5 40.0 21.5 41.0 56-57 33.59805041334735 37.5 33.0 40.0 19.5 41.0 58-59 33.17228125261454 37.0 33.0 40.0 17.5 41.0 60-61 32.662418989635746 36.5 32.0 39.5 12.5 41.0 62-63 32.1077540533102 36.0 31.0 39.0 8.0 41.0 64-65 31.560487801738645 35.0 31.0 39.0 6.5 40.0 66-67 31.007296280157775 35.0 30.5 38.0 2.0 40.0 68-69 30.337763105043642 35.0 29.5 37.5 2.0 40.0 70-71 29.756181470787432 34.5 29.0 37.0 2.0 39.0 72-73 29.085719650665933 34.0 28.0 36.0 2.0 39.0 74-75 28.43337397969247 34.0 26.5 36.0 2.0 38.5 76-77 27.717673746149416 33.5 26.0 35.0 2.0 37.5 78-79 26.98322987503611 33.0 24.5 35.0 2.0 37.0 80-81 26.29897731690872 32.5 23.0 35.0 2.0 36.0 82-83 25.581541694570817 32.0 20.0 35.0 2.0 36.0 84-85 24.882277114787414 31.0 18.0 34.5 2.0 35.0 86-87 24.165506649105822 31.0 10.0 34.0 2.0 35.0 88-89 23.286383633739078 30.5 4.5 34.0 2.0 35.0 90-91 22.41456144143271 29.5 2.0 34.0 2.0 35.0 92-93 21.500681246103042 29.0 2.0 34.0 2.0 35.0 94-95 20.43967460702946 28.0 2.0 34.0 2.0 35.0 96-97 19.196955270959535 26.0 2.0 33.5 2.0 35.0 98-99 17.649073456085084 22.5 2.0 33.0 2.0 34.5 100-101 13.77456849050325 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17958183939189354 1101 2 0.1386333936298172 1101 3 0.07791826552711001 1101 4 -0.03506800785974207 1101 5 0.009196088972799998 1101 6 0.041930812381814064 1101 7 0.00975423896177574 1101 8 -0.029866599589389864 1101 9 -0.042411389478147044 1101 10-11 0.15554869235702995 1101 12-13 0.11944677805202275 1101 14-15 0.2124575959128876 1101 16-17 0.137788981753161 1101 18-19 0.27329385834664777 1101 20-21 0.15722085904567962 1101 22-23 0.15922321060899947 1101 24-25 0.13185692394907278 1101 26-27 0.26025257758067966 1101 28-29 0.2586009991648268 1101 30-31 0.18623308031105523 1101 32-33 0.21442978459806739 1101 34-35 0.1253937227869102 1101 36-37 0.11344284805029048 1101 38-39 0.2078750354651362 1101 40-41 0.26743483471965845 1101 42-43 0.20225617708156562 1101 44-45 0.28148030782185174 1101 46-47 0.3456330896018187 1101 48-49 0.4388718063656043 1101 50-51 0.36750990678491036 1101 52-53 0.3970632222017869 1101 54-55 0.34962196497044573 1101 56-57 0.2990493205442064 1101 58-59 0.32069420367184875 1101 60-61 0.32807422894673266 1101 62-63 0.21910664354953013 1101 64-65 0.15259946959595538 1101 66-67 0.21810889870436867 1101 68-69 0.17807873108883499 1101 70-71 0.07318908752899134 1101 72-73 -0.015873266141699816 1101 74-75 -0.13595295216090975 1101 76-77 -0.2889995284956761 1101 78-79 -0.4770633522968133 1101 80-81 -0.5131886193207009 1101 82-83 -0.4981419037638162 1101 84-85 -0.6694091248722174 1101 86-87 -0.6594557416275215 1101 88-89 -0.612698857541794 1101 90-91 -0.6630274448505382 1101 92-93 -0.6826408224048599 1101 94-95 -0.6767513276195238 1101 96-97 -0.8248641933082368 1101 98-99 -1.2992772034098827 1101 100-101 -1.617198652954242 1102 1 0.10578245178073331 1102 2 0.1603113777551286 1102 3 0.13091615204766072 1102 4 -0.0192876839058016 1102 5 0.031765073450159775 1102 6 0.14206824298601362 1102 7 0.1023021370356858 1102 8 0.11143286968168553 1102 9 0.1264002108262261 1102 10-11 0.1369359793002758 1102 12-13 0.13869643840728685 1102 14-15 0.17664458379939418 1102 16-17 0.19752985973631354 1102 18-19 0.195852136302463 1102 20-21 0.1385346520698647 1102 22-23 0.18267291233861727 1102 24-25 0.15866950175281858 1102 26-27 0.1358276750584153 1102 28-29 -0.09161355468776833 1102 30-31 -0.11695776555253445 1102 32-33 -0.06905501274623305 1102 34-35 0.0013290312012657068 1102 36-37 0.040432839569760404 1102 38-39 -0.18176764496395492 1102 40-41 0.029770017801176607 1102 42-43 0.19518231176113687 1102 44-45 0.109503629892167 1102 46-47 -0.009116613700506093 1102 48-49 0.06952856980229427 1102 50-51 0.07023607751582261 1102 52-53 0.13120577499945796 1102 54-55 0.029130106161034064 1102 56-57 0.015930783018752948 1102 58-59 -8.350384395043875E-4 1102 60-61 0.04018524045291372 1102 62-63 -0.17795468848692764 1102 64-65 -0.1568949164364497 1102 66-67 -0.1994771980608121 1102 68-69 -0.5338916439055694 1102 70-71 -0.6306313259967915 1102 72-73 -0.6614429438209619 1102 74-75 -0.7129921210928849 1102 76-77 -1.002077453825855 1102 78-79 -0.670537757661446 1102 80-81 -0.5726324971865075 1102 82-83 -0.7720576718690282 1102 84-85 -0.8183852305776433 1102 86-87 -0.61430507002013 1102 88-89 -0.7835244331836684 1102 90-91 -0.8667114560821041 1102 92-93 -0.8990882775796187 1102 94-95 -0.9040389846333845 1102 96-97 -0.7714020550767628 1102 98-99 -0.7906554291275505 1102 100-101 -1.1366814977193584 1103 1 0.26675179439804 1103 2 0.21187588838581917 1103 3 0.18769575993793097 1103 4 0.11396419019442305 1103 5 0.11633819331282069 1103 6 0.18639768252349143 1103 7 0.17946732604819005 1103 8 0.14656568792261737 1103 9 0.30637633689070043 1103 10-11 0.31881717939211285 1103 12-13 0.20044344591184426 1103 14-15 0.3071485070852802 1103 16-17 0.3834779009125526 1103 18-19 0.26712627592640814 1103 20-21 0.28738433715722067 1103 22-23 0.2845511114960573 1103 24-25 0.22615333880912658 1103 26-27 0.13899108279934325 1103 28-29 0.19440368603279978 1103 30-31 0.1828152725541372 1103 32-33 0.18751885122946277 1103 34-35 0.13617865248630778 1103 36-37 -0.06646719518766275 1103 38-39 -0.02356860251797599 1103 40-41 0.21016141532037835 1103 42-43 0.23202749878537787 1103 44-45 0.168544232275039 1103 46-47 0.4419417257255702 1103 48-49 0.5546165409517769 1103 50-51 0.6117508891116685 1103 52-53 0.5797416046354087 1103 54-55 0.47596395343683895 1103 56-57 0.5209116293416045 1103 58-59 0.5417006101759299 1103 60-61 0.4488920120669775 1103 62-63 0.3587336820947158 1103 64-65 0.2024711519351463 1103 66-67 0.13295019805919495 1103 68-69 0.2421984019319403 1103 70-71 0.03619345541536845 1103 72-73 0.1014592194113213 1103 74-75 0.1404875346862191 1103 76-77 0.15818282993864585 1103 78-79 0.04025590602691054 1103 80-81 0.06040302400976927 1103 82-83 -0.07962173291597097 1103 84-85 -0.3634183502845083 1103 86-87 -0.3428260396520848 1103 88-89 -0.13199291799370982 1103 90-91 -0.20269907694946454 1103 92-93 -0.4525228080595056 1103 94-95 -0.6124343119299773 1103 96-97 -0.702177193837521 1103 98-99 -0.9135834768101354 1103 100-101 -1.0947806264839262 1104 1 0.19553232156584954 1104 2 0.14400572983406335 1104 3 0.2604215779349843 1104 4 0.24844622782758563 1104 5 0.25297332492013425 1104 6 0.17815817449708504 1104 7 0.23348957195052833 1104 8 0.3426117840336289 1104 9 0.31724352908280196 1104 10-11 0.24020796458073335 1104 12-13 0.3847238596464493 1104 14-15 0.4825693340381676 1104 16-17 0.44600721019063627 1104 18-19 0.43090853066405543 1104 20-21 0.4395787953593455 1104 22-23 0.41752458145985116 1104 24-25 0.4984047546727126 1104 26-27 0.38285162782241144 1104 28-29 0.28062096114996393 1104 30-31 0.3349143340246812 1104 32-33 0.3779024332809868 1104 34-35 0.32226681718825034 1104 36-37 0.2233012318108436 1104 38-39 0.19873689713779186 1104 40-41 0.24400887663042425 1104 42-43 0.24819117564211268 1104 44-45 0.09919918554517437 1104 46-47 0.06668457768095948 1104 48-49 0.06283698672805116 1104 50-51 0.009349079787853043 1104 52-53 0.2378144162762723 1104 54-55 0.23683264154544048 1104 56-57 0.21791553941087471 1104 58-59 0.25207425250349047 1104 60-61 0.2436125210136595 1104 62-63 0.27521982725811256 1104 64-65 0.11655374396350027 1104 66-67 0.10484406423741177 1104 68-69 0.2571966316216283 1104 70-71 0.09507383971032723 1104 72-73 0.12604182879230663 1104 74-75 0.11090121286790477 1104 76-77 -0.028514728400814704 1104 78-79 -0.14296633912319834 1104 80-81 -0.13598439750376556 1104 82-83 -0.27672437347356293 1104 84-85 -0.2739316000870069 1104 86-87 -0.03726706010989389 1104 88-89 -0.19121616634815908 1104 90-91 -0.3055158107671083 1104 92-93 -0.45061085582352334 1104 94-95 -0.48388815640971217 1104 96-97 -0.6455887245669771 1104 98-99 -0.8323490567803802 1104 100-101 -0.9368500551310177 1105 1 0.19081936669342525 1105 2 0.1939554562509116 1105 3 0.2674944329871707 1105 4 0.07323989751229476 1105 5 0.13880983284810355 1105 6 0.09404278501087049 1105 7 0.16430370084029988 1105 8 0.15926046499790658 1105 9 0.24402664400435015 1105 10-11 0.22436872986707357 1105 12-13 0.27562562055020834 1105 14-15 0.16051320723758522 1105 16-17 0.2037354798845783 1105 18-19 0.26736039083553464 1105 20-21 0.13011567550253034 1105 22-23 0.19128816017618533 1105 24-25 0.18571276524540536 1105 26-27 0.17232299160153985 1105 28-29 0.2815776994644281 1105 30-31 0.09670287399475086 1105 32-33 0.18897831729822911 1105 34-35 0.08911400912870704 1105 36-37 0.13030439328496612 1105 38-39 0.08289169707109068 1105 40-41 0.20902297751889165 1105 42-43 0.13977944110814633 1105 44-45 0.18485986224764872 1105 46-47 0.12511887787415077 1105 48-49 0.23354053492329996 1105 50-51 0.16320792160640707 1105 52-53 0.2904624055203797 1105 54-55 0.22558412086334556 1105 56-57 0.22009321924385006 1105 58-59 0.08117691182853548 1105 60-61 0.13487896709266067 1105 62-63 0.1700050981821235 1105 64-65 -0.08254072848496108 1105 66-67 -0.05413593536815853 1105 68-69 -0.11201821871727802 1105 70-71 -0.24374590704250565 1105 72-73 -0.23568202132477367 1105 74-75 -0.2822891410584454 1105 76-77 -0.2884547423753858 1105 78-79 -0.3041059269173054 1105 80-81 -0.23484301032448585 1105 82-83 -0.31131510869354884 1105 84-85 -0.22916773760892895 1105 86-87 -0.23631206576475705 1105 88-89 -0.24392511302491116 1105 90-91 -0.21974299846661793 1105 92-93 -0.4136492187481018 1105 94-95 -0.4251256803057579 1105 96-97 -0.6511617659957025 1105 98-99 -0.8309411682692449 1105 100-101 -1.2771401147177226 1106 1 0.2144400117628038 1106 2 0.2402991391658631 1106 3 0.263914843512147 1106 4 0.2507132080209047 1106 5 0.20098784400031633 1106 6 0.19988146450108957 1106 7 0.3900681767569125 1106 8 0.18277684516837667 1106 9 0.371196270090806 1106 10-11 0.4506483363238587 1106 12-13 0.49785076769533276 1106 14-15 0.5242673733171088 1106 16-17 0.1224914447916916 1106 18-19 0.4993028200941225 1106 20-21 0.5575189169020973 1106 22-23 0.5379674005702597 1106 24-25 0.533737384149461 1106 26-27 0.47316000130488334 1106 28-29 0.4545763940885408 1106 30-31 0.32531114922104365 1106 32-33 0.4961321281544784 1106 34-35 0.43556665402465455 1106 36-37 0.4614657132785851 1106 38-39 0.4812339367946805 1106 40-41 0.368234187750474 1106 42-43 0.2130092734636051 1106 44-45 0.4923324036735863 1106 46-47 0.5870655448298692 1106 48-49 0.6463807572243141 1106 50-51 0.5518372666008915 1106 52-53 0.45237108288917227 1106 54-55 0.28094387863843195 1106 56-57 0.21697275663805016 1106 58-59 0.23405583602124835 1106 60-61 0.5229160151985184 1106 62-63 0.4954161057681645 1106 64-65 0.4498706863535915 1106 66-67 0.151802208706318 1106 68-69 0.16190038136643992 1106 70-71 0.10168086415746203 1106 72-73 0.1591812806826347 1106 74-75 0.21033153652919623 1106 76-77 -0.15397031423480811 1106 78-79 -0.044270572548267495 1106 80-81 0.03692912648138602 1106 82-83 0.12086934933286386 1106 84-85 0.13326556769676046 1106 86-87 -0.041369739222577806 1106 88-89 -0.40856042073282595 1106 90-91 -0.3904796093309173 1106 92-93 -0.25773680539249355 1106 94-95 -0.5946818266957443 1106 96-97 -0.6682284330752992 1106 98-99 -0.912904992478623 1106 100-101 -1.0435214465521199 1107 1 0.010915749857808521 1107 2 -0.05984114827325726 1107 3 0.04358349662184935 1107 4 -0.05299855033301526 1107 5 -0.08298343456779378 1107 6 -0.07633681973626238 1107 7 -0.018118228926766733 1107 8 0.016840717141725747 1107 9 0.038722430210647474 1107 10-11 0.07481827151386256 1107 12-13 0.050572653505888354 1107 14-15 -0.023143498890128456 1107 16-17 0.1269482771102446 1107 18-19 0.08750806626847663 1107 20-21 0.03651922223629356 1107 22-23 0.08832714215559889 1107 24-25 0.07667979885797394 1107 26-27 0.06254878320618928 1107 28-29 0.05378352600569514 1107 30-31 -0.0649912640585768 1107 32-33 -0.009764609758377674 1107 34-35 -0.014248521008212833 1107 36-37 0.05683237839009081 1107 38-39 -0.08606677181779077 1107 40-41 -0.024992535751771072 1107 42-43 0.020925343666149843 1107 44-45 -0.11044658409811348 1107 46-47 -0.09470900581843011 1107 48-49 -0.19940853322857066 1107 50-51 -0.10479548379509396 1107 52-53 -0.10972262283794976 1107 54-55 -0.1339162273308503 1107 56-57 -0.20734970707484024 1107 58-59 -0.30155594024007115 1107 60-61 -0.2936157762817757 1107 62-63 -0.23918711297476847 1107 64-65 -0.24771688089346355 1107 66-67 -0.26582122568601463 1107 68-69 -0.20854730201230964 1107 70-71 -0.16658772154159251 1107 72-73 -0.2460605669480529 1107 74-75 -0.23831965771555375 1107 76-77 -0.5488978554876454 1107 78-79 -0.49016490535762003 1107 80-81 -0.5474408110798485 1107 82-83 -0.6899390810938222 1107 84-85 -0.8276154700638294 1107 86-87 -0.7059208540619579 1107 88-89 -0.8194491803846127 1107 90-91 -0.7138119772353804 1107 92-93 -1.1357105226308484 1107 94-95 -1.2118768077590154 1107 96-97 -1.0703164694099172 1107 98-99 -0.9475983795064309 1107 100-101 -1.1494810374258453 1108 1 -0.11056610973869851 1108 2 -0.05933502535827273 1108 3 -0.029686460064631603 1108 4 0.06621327408463173 1108 5 0.010071697661281576 1108 6 0.09071827375631614 1108 7 -0.016728174828195108 1108 8 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-0.15454450256484265 2316 88-89 -0.2423555687965937 2316 90-91 -0.14270593373957396 2316 92-93 -0.16229779324250515 2316 94-95 -0.20290655324073015 2316 96-97 -0.5632090603171136 2316 98-99 -0.7728025542702497 2316 100-101 -0.4953039468594014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8506.0 3 2479.0 4 2407.0 5 2675.0 6 3273.0 7 4209.0 8 5130.0 9 6465.0 10 8214.0 11 10237.0 12 13118.0 13 15962.0 14 18818.0 15 23425.0 16 28115.0 17 35607.0 18 34330.0 19 29212.0 20 28947.0 21 30696.0 22 33314.0 23 36991.0 24 40566.0 25 45648.0 26 51545.0 27 59002.0 28 67960.0 29 78646.0 30 92457.0 31 111015.0 32 135061.0 33 169680.0 34 219054.0 35 290909.0 36 358781.0 37 362099.0 38 167658.0 39 9272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.53517409338485 17.976071281936772 14.6127136336978 22.876040990980574 2 15.98141744093996 19.317755699573127 39.75319210184424 24.94763475764267 3 19.51129441614742 23.583524992246442 29.293785246472225 27.61139534513391 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29.801862312950355 22.407533912734756 24-25 22.64825930508783 25.24827573255402 29.659297006881513 22.444167955476637 26-27 21.857940693017447 25.72290183260688 29.550745635727278 22.8684118386484 28-29 22.275194999521446 26.165538507608083 29.320858670714962 22.23840782215551 30-31 22.19064565067832 25.644891842716213 29.238286316157247 22.92617619044822 32-33 21.859556196397993 25.680067500722085 29.288520859345446 23.17185544353448 34-35 22.85583340027677 25.784185485349997 28.999270190265868 22.360710924107366 36-37 23.055194546853038 25.176814273968347 28.970961007271118 22.797030171907505 38-39 22.35729384890251 25.63110686726539 28.927645755205265 23.08395352862683 40-41 22.818778218067063 25.432350315216574 29.067483203159476 22.68138826355689 42-43 22.438605669010602 25.5452843918676 28.79007644720688 23.226033491914915 44-45 22.47818354379084 25.806854771821946 29.054005433946944 22.660956250440268 46-47 23.14823038974965 26.214427359724958 28.076750373606586 22.560591876918814 48-49 22.808134144530932 26.35111438673886 27.7689048832439 23.071846585486313 50-51 23.01459300231317 26.603300404037572 27.808367937438756 22.5737386562105 52-53 23.440944744732466 26.072005507426166 27.837267371453628 22.64978237638774 54-55 22.677757484256528 26.523137770619915 27.98402510168014 22.81507964344342 56-57 22.796286517448532 26.67992522747286 27.70923082486639 22.81455743021221 58-59 22.7664477509277 26.06114919210509 27.723622256909024 23.448780800058177 60-61 22.300841353254754 26.49011099379362 28.121804592146667 23.087243060804965 62-63 22.55007737280549 26.449530413070978 28.28306989081448 22.71732232330905 64-65 22.22983240657194 26.532896163274117 28.08275428498931 23.154517145164636 66-67 22.214506899007514 26.54595920413969 27.989290618004965 23.250243278847833 68-69 22.85913708227816 26.062982879157804 28.253518779133746 22.82436125943029 70-71 22.617705440620924 26.351291847524816 27.71670734416497 23.314295367689294 72-73 22.623991260988394 26.533498250360637 27.525178349204797 23.317332139446172 74-75 22.645759866199707 27.022447377301447 27.384558049624424 22.947234706874422 76-77 22.915548446912272 26.496066801051832 27.57834259432284 23.010042157713052 78-79 22.982720800811535 26.755893114561168 27.43794683748586 22.823439247141433 80-81 23.244395318438006 26.79383132865904 27.07075205200793 22.891021300895023 82-83 23.044865617465963 26.933058834176283 27.15608624382841 22.86598930452935 84-85 22.959073846781234 27.084877499466582 27.140746731763503 22.815301921988677 86-87 23.3590220417752 27.006201414772296 26.742214340918398 22.89256220253411 88-89 23.179012912744035 27.209593006021578 26.515172895681854 23.096221185552533 90-91 23.102712177558804 27.165682071203545 26.588227491294976 23.143378259942676 92-93 23.27592576104027 26.567973129391525 26.905674388998662 23.250426720569543 94-95 23.082772084572046 26.36144104510663 27.047877610199805 23.507909260121515 96-97 23.632507846021927 25.861310327806407 26.97211269329039 23.534069132881278 98-99 23.134801415367797 26.392503391545635 26.49555183378311 23.97714335930346 100-101 24.29462310688227 25.118253817982083 26.649578452170907 23.937544622964733 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1452.0 1 990.5 2 761.5 3 1036.0 4 863.5 5 773.0 6 1449.5 7 3201.0 8 5291.0 9 6079.0 10 4895.0 11 3460.0 12 3132.5 13 3239.0 14 3465.5 15 3638.0 16 3786.0 17 4039.5 18 4404.5 19 4861.5 20 5412.5 21 6229.5 22 7279.0 23 8678.0 24 10557.5 25 12789.5 26 15641.0 27 19117.5 28 21995.5 29 24887.0 30 28277.5 31 33790.0 32 42123.5 33 44304.0 34 42484.0 35 44683.5 36 49395.5 37 54218.5 38 58266.0 39 61439.0 40 64882.5 41 69821.5 42 72575.0 43 74716.5 44 77841.5 45 83797.0 46 87701.5 47 88987.5 48 90107.0 49 89753.5 50 90981.5 51 91797.5 52 93408.5 53 97776.0 54 102308.5 55 98818.5 56 91467.5 57 85643.5 58 81255.0 59 73496.5 60 59702.0 61 49681.0 62 42595.0 63 32755.5 64 23810.5 65 18399.0 66 13792.5 67 10308.0 68 8011.5 69 6122.0 70 4789.0 71 3493.0 72 2439.5 73 1948.0 74 1499.5 75 1077.5 76 792.0 77 518.5 78 342.0 79 213.0 80 119.0 81 82.5 82 47.5 83 34.5 84 30.5 85 22.0 86 16.5 87 12.5 88 10.5 89 6.5 90 5.0 91 4.0 92 2.0 93 0.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14128427099474045 2 0.05197837729790424 3 0.15086222398554147 4 0.13030558970093695 5 0.19606410489864975 6 0.19719983054973284 7 0.16445307427683614 8 0.06943069480288155 9 0.12212836501313845 10-11 0.10053064888170773 12-13 0.15004828726893188 14-15 0.1376120913895717 16-17 0.13454563213164727 18-19 0.13074095120051882 20-21 0.13337204895886137 22-23 0.19246764033688651 24-25 0.21402749894661446 26-27 0.1477011209233601 28-29 0.12627376363959186 30-31 0.097350617058675 32-33 0.12451338888041302 34-35 0.09233449543305787 36-37 0.08605014683039793 38-39 0.04997192864765739 40-41 0.05875487368270021 42-43 0.061404900201894166 44-45 0.03956111017939544 46-47 0.05071015032086142 48-49 0.041908276524967224 50-51 0.052319094993229176 52-53 0.025932402366397964 54-55 0.05076693660341558 56-57 0.025497040866816104 58-59 0.05110765429874052 60-61 0.03691108366020149 62-63 0.0508615804076725 64-65 0.0384443132891637 66-67 0.03764930533340551 68-69 0.06503922228536016 70-71 0.053284461796649835 72-73 0.051770160928539 74-75 0.04277899952413095 76-77 0.021446286044619633 78-79 0.021275927196957166 80-81 0.024796676715314842 82-83 0.020897351979929455 84-85 0.018720544482020138 86-87 0.024001668759556657 88-89 0.0653610112198337 90-91 0.11056289213294199 92-93 0.08207510705160699 94-95 0.07503360801489163 96-97 0.06698888465305285 98-99 0.055366625490302225 100-101 0.12899950520219133 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2641483.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.06491130275275 #Duplication Level Percentage of deduplicated Percentage of total 1 74.26843950237641 35.697059572755805 2 13.74040962395192 13.208631396774784 3 4.898310069650295 7.063105170933662 4 2.2733741805789474 4.370781133899813 5 1.237787135292989 2.974706443477297 6 0.7701118256518824 2.220921395589523 7 0.5058986854869228 1.7021182812275708 8 0.36457427515744517 1.4018584158966383 9 0.27788168772265465 1.2020722805743769 >10 1.4612661812017036 12.989870784087524 >50 0.12428789429586894 4.115383058148185 >100 0.06875272823798964 6.103390632449382 >500 0.004098433102102601 1.33915877151603 >1k 0.004571329229262547 4.335161900341218 >5k 7.881602119418185E-5 0.27586291864697077 >10k+ 1.576320423883637E-4 0.9999178436812405 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14614 0.5532498221642917 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11781 0.4459994631803422 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7282 0.27567847303957665 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4886 0.1849718510397379 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4560 0.17263029896463464 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4326 0.1637716388861863 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4043 0.15305796024430218 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3979 0.15063507885532484 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3656 0.13840709934532988 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3355 0.12701198531279587 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3203 0.12125764201397474 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3171 0.12004620131948605 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3092 0.11705545710496718 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1357256510831227E-4 2 0.0 0.0 0.0 0.0 1.1357256510831227E-4 3 0.0 0.0 0.0 0.0 1.1357256510831227E-4 4 0.0 0.0 0.0 0.0 1.1357256510831227E-4 5 0.0 0.0 0.0 0.0 1.1357256510831227E-4 6 0.0 0.0 0.0 0.0 1.1357256510831227E-4 7 0.0 0.0 0.0 0.0 1.1357256510831227E-4 8 0.0 0.0 0.0 0.0 1.1357256510831227E-4 9 0.0 0.0 0.0 3.785752170277075E-5 1.1357256510831227E-4 10-11 0.0 0.0 0.0 3.785752170277075E-5 1.51430086811083E-4 12-13 0.0 0.0 0.0 3.785752170277075E-5 6.435778689471029E-4 14-15 0.0 0.0 0.0 3.785752170277075E-5 8.328654774609565E-4 16-17 0.0 0.0 0.0 3.785752170277075E-5 9.085805208664981E-4 18-19 0.0 0.0 0.0 7.57150434055415E-5 9.464380425692688E-4 20-21 0.0 0.0 0.0 1.8928760851385379E-4 9.842955642720395E-4 22-23 0.0 0.0 0.0 4.353614995818636E-4 0.0010600106076775811 24-25 0.0 0.0 0.0 6.814353906498735E-4 0.0010600106076775811 26-27 0.0 0.0 0.0 0.0011925119336372789 0.0011357256510831227 28-29 0.0 0.0 0.0 0.0037100371268715343 0.0011357256510831227 30-31 0.0 0.0 0.0 0.011603330401899235 0.0011735831727858934 32-33 0.0 0.0 0.0 0.02865814392899746 0.001211440694488664 34-35 0.0 0.0 0.0 0.05315196047069014 0.0012682269770428202 36-37 0.0 0.0 0.0 0.09379201001861454 0.0012871557378942057 38-39 0.0 0.0 0.0 0.15536726906817117 0.0013060844987455912 40-41 0.0 0.0 0.0 0.22070935152715349 0.0013250132595969764 42-43 0.0 0.0 0.0 0.28894753439639775 0.001362870781299747 44-45 0.0 0.0 0.0 0.371969079490574 0.0014385858247052887 46-47 0.0 0.0 0.0 0.4686761186803019 0.0015143008681108303 48-49 0.0 0.0 0.0 0.5675410366070878 0.0015710871506649862 50-51 0.0 0.0 0.0 0.6750185407212539 0.0017414459983274547 52-53 0.0 0.0 0.0 0.7744134639518785 0.0018171610417329963 54-55 0.0 0.0 0.0 0.8827049047826543 0.0019307336068413086 56-57 0.0 0.0 0.0 1.0091490272699086 0.0019307336068413086 58-59 3.785752170277075E-5 0.0 0.0 1.1412149917300245 0.001968591128544079 60-61 3.785752170277075E-5 0.0 0.0 1.2865500175469613 0.0020632349328010063 62-63 3.785752170277075E-5 0.0 0.0 1.4260171274999687 0.002157878737057933 64-65 3.785752170277075E-5 0.0 0.0 1.5652192348010567 0.002157878737057933 66-67 3.785752170277075E-5 0.0 0.0 1.7314326838370717 0.002157878737057933 68-69 3.785752170277075E-5 0.0 0.0 1.912296993772059 0.002157878737057933 70-71 3.785752170277075E-5 0.0 0.0 2.098631715593097 0.002176807497909318 72-73 3.785752170277075E-5 0.0 0.0 2.2917240050380787 0.00225252254131486 74-75 3.785752170277075E-5 0.0 0.0 2.4844377192660336 0.002309308823869016 76-77 3.785752170277075E-5 0.0 0.0 2.6890576240695094 0.002309308823869016 78-79 3.785752170277075E-5 0.0 0.0 2.9184552768274488 0.0023282375847204014 80-81 3.785752170277075E-5 0.0 0.0 3.1562762281642547 0.002347166345571787 82-83 3.785752170277075E-5 0.0 0.0 3.4060601563591364 0.0024985964323828696 84-85 3.785752170277075E-5 0.0 0.0 3.6622420057217857 0.0024985964323828696 86-87 3.785752170277075E-5 0.0 0.0 3.9189538603882745 0.0026310977583425673 88-89 7.57150434055415E-5 0.0 0.0 4.190335504714586 0.002725741562599494 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5735 0.0 61.187122 1 GTATCAA 11335 0.0 47.760067 1 TGGTATC 2155 0.0 37.735233 2 ATCAACG 14625 0.0 36.938618 3 TATCAAC 14780 0.0 36.55124 2 GTGGTAT 2355 0.0 36.39214 1 TCAACGC 14880 0.0 36.273636 4 CAACGCA 15175 0.0 35.568485 5 AACGCAG 15680 0.0 34.448696 6 ACGCAGA 17800 0.0 30.234379 7 CGCAGAG 17930 0.0 29.958723 8 AGGTAAG 1735 0.0 27.683443 2 GCAGAGT 19350 0.0 27.14501 9 TAAGGTG 1965 0.0 26.379234 5 TATACCG 145 7.814433E-7 26.237362 5 TAAGGTA 835 0.0 25.628561 4 GTAAGGT 2045 0.0 24.64965 4 GTACATG 11340 0.0 24.352352 1 GGTAAGG 2050 0.0 24.125578 3 TACATGG 11445 0.0 23.933428 2 >>END_MODULE