FastQCFastQC Report
Thu 26 May 2016
SRR938268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641483
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141760.5366682276584782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121560.46019603381888124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76510.2896478985478991No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47740.18073180860902757No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC45410.171911006052282No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA44760.1694502671416019No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA43760.16566451497132484No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA41490.15707085754479586No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35410.13405348434951125No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG34170.12935915165836767No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA33480.12674698266087647No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33190.12564911453149613No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30730.11633616419261451No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA28330.10725035898394954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60000.059.696381
GTATCAA113000.044.6871571
TGGTATC24200.035.5249522
ATCAACG145350.034.6680643
TATCAAC146300.034.4786722
TCAACGC147950.034.088994
CAACGCA150000.033.6864245
GTGGTAT26500.033.5215151
AACGCAG156200.032.4703186
ACGCAGA176050.028.7013247
CGCAGAG176650.028.523198
TAAGGTG20400.027.0042675
GTGTAAG12200.026.0881231
GCAGAGT193100.025.8228029
TAAGGTA9750.024.353984
AGGTAAG18850.023.937712
GTACATG112900.023.814991
GAGTACT123250.023.67725212-13
TACATGG113400.023.4555322
CAGAGTA184250.022.69437210-11
Produced by FastQC (version 0.11.5)