##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938268_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2641483 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51338585181127 34.0 31.0 34.0 31.0 34.0 2 32.72463650154099 34.0 31.0 34.0 31.0 34.0 3 32.77476137457633 34.0 31.0 34.0 31.0 34.0 4 36.23809617551959 37.0 35.0 37.0 35.0 37.0 5 36.21008993811431 37.0 37.0 37.0 35.0 37.0 6 36.24700140034973 37.0 37.0 37.0 35.0 37.0 7 36.20563410780989 37.0 37.0 37.0 35.0 37.0 8 36.1872077162715 37.0 37.0 37.0 35.0 37.0 9 37.876502328426874 39.0 38.0 39.0 35.0 39.0 10-11 37.855010613356214 39.0 38.0 39.0 35.0 39.0 12-13 37.77345055788737 39.0 38.0 39.0 35.0 39.0 14-15 39.037746409876576 40.0 38.0 41.0 35.5 41.0 16-17 38.92556567655366 40.0 38.0 41.0 35.5 41.0 18-19 39.18506308766705 40.0 39.0 41.0 36.0 41.0 20-21 39.166624581721706 40.0 39.0 41.0 36.0 41.0 22-23 39.07551685927943 40.0 39.0 41.0 35.5 41.0 24-25 38.965566123272424 40.0 38.0 41.0 35.0 41.0 26-27 38.78414152201623 40.0 38.0 41.0 35.0 41.0 28-29 38.66201145341462 40.0 38.0 41.0 34.5 41.0 30-31 38.39637222726779 40.0 38.0 41.0 34.0 41.0 32-33 38.162323777968666 40.0 38.0 41.0 34.0 41.0 34-35 37.99213491057864 40.0 37.0 41.0 33.0 41.0 36-37 37.972272393954455 40.0 37.0 41.0 33.0 41.0 38-39 37.80533719126718 40.0 37.0 41.0 33.0 41.0 40-41 37.60890624698323 40.0 37.0 41.0 33.0 41.0 42-43 37.35157352896081 40.0 37.0 41.0 32.5 41.0 44-45 37.05633861736002 40.0 36.0 41.0 31.5 41.0 46-47 36.62382419269781 39.5 36.0 41.0 30.5 41.0 48-49 36.17835833128587 39.0 35.0 41.0 30.0 41.0 50-51 35.76221444544599 39.0 35.0 40.0 28.5 41.0 52-53 35.22040005557484 38.0 34.5 40.0 27.0 41.0 54-55 34.883375172204396 38.0 34.0 40.0 26.0 41.0 56-57 34.509926052902856 38.0 34.0 40.0 26.0 41.0 58-59 34.04082062992644 37.0 33.5 40.0 24.5 41.0 60-61 33.5367242567906 36.5 33.0 39.5 23.0 41.0 62-63 33.05180991132633 36.0 32.5 39.0 22.0 40.5 64-65 32.54385093525114 35.5 32.0 39.0 20.5 40.0 66-67 31.97909110147595 35.0 31.0 38.0 18.5 40.0 68-69 31.387808098708188 35.0 31.0 37.5 14.5 40.0 70-71 30.759808599941778 34.5 30.0 37.0 9.0 39.0 72-73 30.18132995745193 34.0 29.0 36.0 7.0 39.0 74-75 29.596765150485542 34.0 29.0 36.0 4.5 38.5 76-77 28.275396055927672 32.5 27.0 34.5 2.0 36.5 78-79 28.96547072231773 34.0 29.0 35.0 2.0 37.0 80-81 28.65526656806044 34.0 28.5 35.0 2.0 36.5 82-83 28.096596116651142 33.0 27.0 35.0 2.0 36.0 84-85 27.513430712974493 33.0 26.0 35.0 2.0 36.0 86-87 26.881707926948614 32.5 25.0 35.0 2.0 35.0 88-89 26.1195796073645 32.0 24.0 34.5 2.0 35.0 90-91 25.55893829337535 32.0 21.5 34.0 2.0 35.0 92-93 24.735874128283243 31.0 18.5 34.0 2.0 35.0 94-95 23.8938624628665 31.0 9.5 34.0 2.0 35.0 96-97 22.825949476108686 30.5 2.0 34.0 2.0 35.0 98-99 21.348926909618573 30.0 2.0 34.0 2.0 35.0 100-101 17.014737933198887 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08755549464031276 1101 2 0.05875619270255328 1101 3 0.0739411196517068 1101 4 0.048793783316696704 1101 5 0.07279753490036711 1101 6 0.04591269419988464 1101 7 0.03090945298498582 1101 8 0.08016854061672518 1101 9 0.005809341659912093 1101 10-11 0.013360896955468604 1101 12-13 0.17285943984525431 1101 14-15 0.09698762271952432 1101 16-17 0.16533507592497898 1101 18-19 0.17335677650287096 1101 20-21 0.19090765263853626 1101 22-23 0.285289338233504 1101 24-25 0.23529474545125595 1101 26-27 0.15265027058030256 1101 28-29 0.07933975997297438 1101 30-31 0.1207396089351036 1101 32-33 0.13258511553883068 1101 34-35 0.1128736078062289 1101 36-37 0.26788061388170803 1101 38-39 0.2333988555551585 1101 40-41 0.26914819445816107 1101 42-43 0.2746404881519382 1101 44-45 0.006312934233662304 1101 46-47 0.05949066475960052 1101 48-49 0.2681443781415993 1101 50-51 0.11851173209925747 1101 52-53 0.2034994190508712 1101 54-55 0.03958113838707078 1101 56-57 0.018902975691794666 1101 58-59 0.01735462085719064 1101 60-61 -0.1137963904529471 1101 62-63 -0.1140893189269292 1101 64-65 0.0017061089363821225 1101 66-67 -0.18224799991643081 1101 68-69 -0.21133064597759343 1101 70-71 -0.2572439471140555 1101 72-73 -0.4345951284377172 1101 74-75 -0.50977274452503 1101 76-77 -0.5796167081073769 1101 78-79 -0.4207695851674238 1101 80-81 -0.4170783989671314 1101 82-83 -0.3470866808805866 1101 84-85 -0.3416253916446905 1101 86-87 -0.6078996970256689 1101 88-89 -0.587945432737623 1101 90-91 -0.6688283856820618 1101 92-93 -0.8234930232845841 1101 94-95 -0.6537652069148621 1101 96-97 -0.6282939551807978 1101 98-99 -0.6294661464714189 1101 100-101 -0.9832005316555659 1102 1 0.17166459711926763 1102 2 0.13406417154114791 1102 3 0.1060915335767163 1102 4 0.029073217480060976 1102 5 0.11257498442547131 1102 6 0.10369101234447697 1102 7 0.046916610022435634 1102 8 0.16003011799583788 1102 9 0.0771419950988772 1102 10-11 0.12782359289388268 1102 12-13 0.21023185379687703 1102 14-15 0.26953985179311246 1102 16-17 0.2532707013007709 1102 18-19 0.19347777834294533 1102 20-21 0.13219599196630583 1102 22-23 0.1873416029955024 1102 24-25 0.22145581024808791 1102 26-27 0.12988379009823348 1102 28-29 0.09882048762931106 1102 30-31 0.11868631586313683 1102 32-33 -0.01850779650195733 1102 34-35 0.035689265700810324 1102 36-37 0.10791278183739195 1102 38-39 0.02284422144600029 1102 40-41 0.0573066606551933 1102 42-43 0.1875420280763791 1102 44-45 0.003679855800228893 1102 46-47 -0.02266858053346965 1102 48-49 0.02251046133308421 1102 50-51 0.04737687710911587 1102 52-53 0.17048824918494887 1102 54-55 0.1181694260573849 1102 56-57 0.28854399872714254 1102 58-59 0.14395621364885614 1102 60-61 0.004507648143764698 1102 62-63 -6.933433692566382E-4 1102 64-65 -0.027044530732908356 1102 66-67 -0.16701358758348306 1102 68-69 -0.04017480136612406 1102 70-71 -0.2569366638969228 1102 72-73 -0.34970813970436865 1102 74-75 -0.4364796483562685 1102 76-77 -0.49716348713808145 1102 78-79 -0.616564644198462 1102 80-81 -0.6436674100837649 1102 82-83 -0.522113810117375 1102 84-85 -0.6855303564462467 1102 86-87 -0.8004779604883687 1102 88-89 -0.6446189225434438 1102 90-91 -0.8537823355416663 1102 92-93 -0.9946809269718635 1102 94-95 -1.006216032526794 1102 96-97 -1.14532579286751 1102 98-99 -1.0649886912603677 1102 100-101 -1.0047578405804494 1103 1 0.14493420974648785 1103 2 0.15292981091637614 1103 3 0.14426627550191995 1103 4 0.09397018601698903 1103 5 0.07436778492787965 1103 6 0.13893047689468574 1103 7 0.08187044793562848 1103 8 0.04561569155082168 1103 9 0.12677815498599188 1103 10-11 0.06293506332578147 1103 12-13 0.1674179046821891 1103 14-15 0.22736188562539894 1103 16-17 0.24797325454324692 1103 18-19 0.19822029334854108 1103 20-21 0.19973537783936735 1103 22-23 0.24775108030988235 1103 24-25 0.21278824738148217 1103 26-27 0.22775589019961018 1103 28-29 0.242146357575713 1103 30-31 0.24451050636199767 1103 32-33 0.2521517265312312 1103 34-35 0.16385809849312238 1103 36-37 0.14718746452289366 1103 38-39 0.14550556061946907 1103 40-41 0.17716190359177375 1103 42-43 0.1705704271539119 1103 44-45 0.24929761984814292 1103 46-47 0.14972425105402465 1103 48-49 0.2198180964298473 1103 50-51 0.24685907682000163 1103 52-53 0.04703077212874973 1103 54-55 0.22118345477658607 1103 56-57 0.14373166470126364 1103 58-59 0.3283231073701529 1103 60-61 0.3217121646056853 1103 62-63 0.2162525140615088 1103 64-65 0.17774225640142305 1103 66-67 0.19283841701473392 1103 68-69 0.372362520205332 1103 70-71 0.09560506077606234 1103 72-73 -0.1753139742033909 1103 74-75 -0.11958524081454058 1103 76-77 -0.044248760043203106 1103 78-79 -0.0087081311507049 1103 80-81 -0.13504844083981737 1103 82-83 -0.20911904079427046 1103 84-85 -0.046237478973026214 1103 86-87 -0.09244124299870649 1103 88-89 -0.289236789342759 1103 90-91 -0.22773780449423597 1103 92-93 -0.3203975808966142 1103 94-95 -0.07168425433776449 1103 96-97 -0.14841964866760549 1103 98-99 -0.1985145072715575 1103 100-101 -0.7996114235440466 1104 1 0.088547219056899 1104 2 0.1743316744351091 1104 3 0.15470384047812047 1104 4 0.10042348427349879 1104 5 0.03156867386129392 1104 6 0.04753818553187017 1104 7 0.039219047937301355 1104 8 0.08009921655236951 1104 9 0.10808928561335307 1104 10-11 0.13483496445852694 1104 12-13 0.13584252681796016 1104 14-15 0.1888050227623097 1104 16-17 0.10555747421421557 1104 18-19 0.18472174131022 1104 20-21 0.12129216701788437 1104 22-23 0.1209646354674021 1104 24-25 0.20853138193686505 1104 26-27 0.23570356628907518 1104 28-29 0.07103035087605747 1104 30-31 -0.02533214202906464 1104 32-33 0.0778240298682249 1104 34-35 0.09561823486600218 1104 36-37 -0.003006141263277584 1104 38-39 -0.09206350157082 1104 40-41 -0.0032718677402172602 1104 42-43 -0.028270639326834157 1104 44-45 0.027724777348659302 1104 46-47 -0.07936552906677008 1104 48-49 -0.09874133769999105 1104 50-51 -0.0424933144324271 1104 52-53 -0.03745876218305 1104 54-55 -0.057740090555746804 1104 56-57 -0.11349929642764067 1104 58-59 -0.17910262208573613 1104 60-61 -0.26087900893994487 1104 62-63 -0.19713973387698758 1104 64-65 -0.2866066344064109 1104 66-67 -0.4342217378049327 1104 68-69 -0.3988419250772104 1104 70-71 -0.4892729844183954 1104 72-73 -0.5159466391726362 1104 74-75 -0.4707471063301085 1104 76-77 -0.5214484283087621 1104 78-79 -0.6046508295139361 1104 80-81 -0.4089837457853882 1104 82-83 -0.5170935408034154 1104 84-85 -0.6117781108857656 1104 86-87 -0.9278824793813776 1104 88-89 -0.7691454341501256 1104 90-91 -0.7465593402892949 1104 92-93 -0.5513063954691333 1104 94-95 -0.4281628635012851 1104 96-97 -0.5806212086264182 1104 98-99 -0.7610773250790182 1104 100-101 -1.407088749296701 1105 1 0.18567783535121407 1105 2 0.16492809401717778 1105 3 0.11810917040696722 1105 4 -0.012974454159163429 1105 5 0.011022107353880983 1105 6 0.07560989820265718 1105 7 0.06212763346571393 1105 8 0.0963135676596849 1105 9 0.1309823873019269 1105 10-11 0.14323486607909075 1105 12-13 0.15569867705619345 1105 14-15 0.13227621279645518 1105 16-17 0.08915821346163 1105 18-19 0.08559753988925678 1105 20-21 0.10446392240776703 1105 22-23 0.20323321032989128 1105 24-25 0.10636737677927499 1105 26-27 0.15545661045273107 1105 28-29 0.24483278588100887 1105 30-31 0.30635957830540406 1105 32-33 0.14956114882111393 1105 34-35 0.21441123206309953 1105 36-37 0.20297252787297992 1105 38-39 0.14136899353261612 1105 40-41 0.10779664922902299 1105 42-43 0.11383898332547204 1105 44-45 0.1346935809971086 1105 46-47 0.07555908989630922 1105 48-49 0.1874045862443623 1105 50-51 0.2085659839475298 1105 52-53 0.17145050887199886 1105 54-55 0.24895239989601947 1105 56-57 0.08547749883801004 1105 58-59 0.07664339013280141 1105 60-61 -0.17235398485443199 1105 62-63 -0.03459894007104225 1105 64-65 -0.03692254548614926 1105 66-67 0.060453940130550166 1105 68-69 0.03555646825437364 1105 70-71 -0.008099703788790436 1105 72-73 -0.07783848080261535 1105 74-75 -0.12124676984182159 1105 76-77 -0.047931173036147356 1105 78-79 -0.1267474098834569 1105 80-81 -0.005108645934992495 1105 82-83 -0.02970977350694426 1105 84-85 -0.18611439609092173 1105 86-87 -0.36379348087777075 1105 88-89 -0.5544007113279719 1105 90-91 -0.6206903097781478 1105 92-93 -0.4505562278769375 1105 94-95 -0.48313293975838434 1105 96-97 -0.9405888653298042 1105 98-99 -1.0604956705606448 1105 100-101 -1.3153557995714245 1106 1 0.20871624870785155 1106 2 0.15957496893491196 1106 3 0.19089174763912808 1106 4 0.17514936092445765 1106 5 0.1356104029479681 1106 6 0.11352309302884578 1106 7 0.16744808543856493 1106 8 0.15236575791938378 1106 9 0.13137621956860812 1106 10-11 0.16509229879855525 1106 12-13 0.11274257103014662 1106 14-15 0.1402669301234738 1106 16-17 0.2588960491804073 1106 18-19 0.25174881793432036 1106 20-21 0.3312994362711308 1106 22-23 0.3197611066100663 1106 24-25 0.34996579587564725 1106 26-27 0.23377038895588953 1106 28-29 0.1921885609434284 1106 30-31 0.2894036281605423 1106 32-33 0.33171089489479044 1106 34-35 0.1958115612717748 1106 36-37 0.14183845611705692 1106 38-39 0.05179486402838762 1106 40-41 0.12446535473900866 1106 42-43 0.2280309845559998 1106 44-45 0.10968703863278506 1106 46-47 -0.036119456258717264 1106 48-49 0.016923217917259592 1106 50-51 -0.07554812314491244 1106 52-53 -0.004349143543926459 1106 54-55 -0.33666069577528646 1106 56-57 -0.16273309354662047 1106 58-59 -0.19215874554267032 1106 60-61 -0.5403190075939861 1106 62-63 -0.4264093968080829 1106 64-65 -0.38257075055558687 1106 66-67 -0.4162198857150692 1106 68-69 -0.530214294525944 1106 70-71 -0.8605424187435275 1106 72-73 -0.9211616302426044 1106 74-75 -0.7683926730829604 1106 76-77 -0.5295328057625284 1106 78-79 -0.5001569185325394 1106 80-81 -0.7026692086144557 1106 82-83 -0.6292254578898735 1106 84-85 -0.7118280815815297 1106 86-87 -0.7039974581191153 1106 88-89 -0.9347234473215025 1106 90-91 -1.254683350870387 1106 92-93 -1.143273376304215 1106 94-95 -1.1429173488525564 1106 96-97 -1.3854748010788853 1106 98-99 -1.2429997624417624 1106 100-101 -1.838585967432751 1107 1 -0.03523977392845268 1107 2 -0.01596432779249568 1107 3 0.018478315582605376 1107 4 -0.03146658300244809 1107 5 -0.04815397820827627 1107 6 -0.009495333420645125 1107 7 -0.019583048333693398 1107 8 -0.06330797208357097 1107 9 -0.06499878749503551 1107 10-11 -0.039582932931317316 1107 12-13 -0.028942276340480078 1107 14-15 -0.06483281315252043 1107 16-17 -0.15948225204355992 1107 18-19 -0.07711397922567897 1107 20-21 -0.008462698684738257 1107 22-23 -0.053418109169136585 1107 24-25 -0.08732228517357044 1107 26-27 -0.0398477813084952 1107 28-29 -0.10833350973058486 1107 30-31 -0.12791939925809714 1107 32-33 -0.10468789836532011 1107 34-35 -0.07997166407056255 1107 36-37 -0.08796633478137039 1107 38-39 -0.09647976676436087 1107 40-41 -0.151797428532376 1107 42-43 -0.12717116038982823 1107 44-45 -0.16569369371661224 1107 46-47 -0.0901818562225003 1107 48-49 -0.14830569881765854 1107 50-51 -0.1696261639802472 1107 52-53 -0.16621199320971414 1107 54-55 -0.10782649193922822 1107 56-57 -0.0020050462366043575 1107 58-59 0.007665706537949291 1107 60-61 -0.14777247261366 1107 62-63 -0.1019156657057394 1107 64-65 -0.24690043265849226 1107 66-67 -0.22244368382926538 1107 68-69 -0.16223114934345872 1107 70-71 -0.34440936324429927 1107 72-73 -0.47576317680730185 1107 74-75 -0.5632140171601172 1107 76-77 -0.4768483360947755 1107 78-79 -0.45826200184658816 1107 80-81 -0.3859096097955721 1107 82-83 -0.35018979968987907 1107 84-85 -0.48746710849697905 1107 86-87 -0.726308642481694 1107 88-89 -0.8246711771239816 1107 90-91 -1.196193758948084 1107 92-93 -1.3588183054699137 1107 94-95 -1.5041560418250768 1107 96-97 -1.6741574341221437 1107 98-99 -1.7443783086413838 1107 100-101 -2.1263469007607263 1108 1 -0.019450446445006264 1108 2 -0.04989023608690246 1108 3 -0.012911371538628202 1108 4 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3.2816675538089406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 21.0 5 49.0 6 112.0 7 269.0 8 669.0 9 1307.0 10 2223.0 11 3718.0 12 5751.0 13 8532.0 14 11433.0 15 16026.0 16 21325.0 17 30538.0 18 30730.0 19 24226.0 20 22340.0 21 22663.0 22 24415.0 23 26358.0 24 29649.0 25 33662.0 26 38436.0 27 45833.0 28 54845.0 29 66408.0 30 79762.0 31 98052.0 32 121906.0 33 157237.0 34 213819.0 35 305377.0 36 420722.0 37 484056.0 38 229450.0 39 9559.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.857206085188245 18.386677737253777 14.96286014902888 22.7932560285291 2 16.24110164148944 19.667431127528655 39.15777540753261 24.933691823449298 3 19.61969651594183 23.428334744907637 29.643211816788888 27.30875692236165 4 13.38226547910055 16.514409908683803 35.41816125120718 34.68516336100846 5 13.855756934238592 37.13305303358978 33.61329235596402 15.39789767620761 6 33.19764961269188 35.44377549038648 16.33114853770883 15.027426359212805 7 29.45697549444763 32.833260709987535 19.350645073241054 18.359118722323785 8 27.594498999236418 33.88460194519518 18.720544482020138 19.80035457354827 9 26.8236819998463 16.430505136697832 19.522063931511198 37.22374893194467 10-11 25.636545834290814 26.27758724928383 25.921991547929707 22.163875368495653 12-13 26.749967726218387 23.485072476881182 26.762782594342603 23.002177202557824 14-15 23.155326004369513 24.857665182778007 25.35969377807845 26.627315034774025 16-17 22.473276633925376 28.113075756744017 26.421785293109945 22.99186231622066 18-19 22.580762397486566 25.88918800537425 29.24637410121511 22.283675495924072 20-21 22.867154549168024 25.01528497438749 29.86078274969023 22.25677772675425 22-23 22.846957561339597 24.873962845871052 29.848157266202357 22.430922326586998 24-25 22.637271027237748 25.273989315093438 29.685076737073423 22.40366292059539 26-27 21.87860958142534 25.730577860558668 29.546631366565634 22.844181191450353 28-29 22.22644361795952 26.11550382358125 29.362325046335968 22.29572751212326 30-31 22.137679561399505 25.61166865393239 29.289507861640875 22.961143923027226 32-33 21.846515764061326 25.61547812346322 29.32967957772206 23.208326534753393 34-35 22.79230545703741 25.786468197965146 29.04976767387072 22.371458671126724 36-37 22.969517420198944 25.17634663715268 29.033910987467344 22.82022495518103 38-39 22.353362521099104 25.607541596730677 28.9823005080443 23.056795374125915 40-41 22.836114693459795 25.381768580464282 29.09552368259009 22.686593043485832 42-43 22.452993017500827 25.52099945880757 28.83800363407214 23.18800388961946 44-45 22.47566991723967 25.72736602885576 29.13989982142607 22.657064232478497 46-47 23.160132516569305 26.20501529255747 28.077771522869178 22.557080668004048 48-49 22.78281780215649 26.251383464450523 27.871922661142968 23.093876072250016 50-51 22.979197206304594 26.564279375598172 27.889081297020944 22.567442121076287 52-53 23.45842093327109 26.036594530886482 27.889075289188643 22.615909246653786 54-55 22.670682643495105 26.458291962979363 28.028002295096304 22.84302309842923 56-57 22.76788503356053 26.690682376585972 27.709614626612378 22.83181796324112 58-59 22.71953163857393 26.102038857322018 27.691157428821185 23.487272075282867 60-61 22.252970038389027 26.54489319976679 28.08259318992345 23.11954357192074 62-63 22.485121848060153 26.53937801997991 28.240279171950426 22.73522096000951 64-65 22.17855058898013 26.558499856678786 27.99720249110064 23.26574706324044 66-67 22.09735190107302 26.631378874273988 27.899221548270823 23.372047676382167 68-69 22.74749315419691 26.117283158169386 28.1825741885863 22.9526494990474 70-71 22.435691733794364 26.482096031178525 27.619258033315177 23.462954201711934 72-73 22.424713602567177 26.58863072515652 27.512023221485098 23.474632450791205 74-75 22.416927626094946 27.050453881033548 27.319096474875295 23.213522017996212 76-77 22.580563934763912 26.501844120029773 27.752922562483402 23.16466938272292 78-79 22.588267458949133 26.76227247029225 27.673546250410386 22.975913820348236 80-81 22.7849845788508 26.82852121288304 27.31226369445755 23.07423051380861 82-83 22.611359437030853 26.912133777126236 27.418122822677365 23.058383963165543 84-85 22.443438434279457 27.087716694141463 27.391892667117396 23.07695220446169 86-87 22.791540041845362 27.06478457212644 26.98976587425563 23.153909511772557 88-89 22.539104514799575 27.26564980507618 26.677669214342743 23.517576465781502 90-91 22.424668036094893 27.164380425873368 26.983523502595748 23.42742803543599 92-93 22.502178275109046 26.647716966992835 27.225807135654378 23.624297622243738 94-95 22.220457388187402 26.417356829311373 27.395659656783742 23.96652612571748 96-97 22.673899527998625 25.97499369579424 27.384049942940315 23.96705683326682 98-99 22.192854174771433 26.463614446883366 26.945271880031463 24.398259498313735 100-101 22.69606724928939 25.701707640510485 27.08343784637199 24.51878726382814 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1517.0 1 1012.5 2 756.0 3 1010.5 4 818.0 5 770.5 6 1537.5 7 3377.0 8 5369.0 9 6091.0 10 4924.0 11 3460.5 12 3118.0 13 3217.5 14 3345.5 15 3545.5 16 3707.0 17 3879.0 18 4181.5 19 4606.0 20 5172.0 21 5997.0 22 7057.0 23 8483.5 24 10235.5 25 12422.5 26 15389.0 27 18857.5 28 21981.0 29 24900.0 30 28362.5 31 33923.0 32 42362.5 33 44621.0 34 42352.5 35 44565.5 36 49361.5 37 53992.0 38 58269.5 39 61938.5 40 65555.0 41 70098.5 42 72664.0 43 75266.0 44 79180.0 45 85351.0 46 88904.5 47 90456.0 48 91437.0 49 90587.5 50 91968.0 51 92591.0 52 94299.0 53 99217.0 54 104485.0 55 100439.0 56 91798.5 57 85066.0 58 80377.0 59 72821.0 60 58377.5 61 48432.5 62 42021.5 63 31619.5 64 22143.0 65 16904.5 66 12536.0 67 9417.0 68 7249.5 69 5527.0 70 4196.5 71 2992.5 72 2158.5 73 1710.5 74 1262.5 75 919.5 76 671.0 77 409.5 78 251.5 79 159.0 80 98.5 81 56.0 82 27.0 83 16.5 84 13.0 85 7.5 86 5.0 87 2.5 88 3.0 89 3.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009464380425692687 2 0.015370153811324925 3 0.008480084861420649 4 7.571504340554152E-5 5 9.464380425692688E-4 6 0.0017414459983274547 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.57150434055415E-4 14-15 0.0 16-17 0.0025743114757884114 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0035396782792090653 26-27 0.01925054978585893 28-29 0.006681852580539038 30-31 0.0030853880187758165 32-33 0.0 34-35 0.009861884403571781 36-37 8.896517600151128E-4 38-39 0.003009672975370275 40-41 0.00225252254131486 42-43 0.0035396782792090653 44-45 0.0 46-47 7.571504340554152E-5 48-49 6.246491080957174E-4 50-51 0.004050754822196471 52-53 0.008101509644392942 54-55 0.0075525755797027656 56-57 0.016430164419002507 58-59 0.02333916212975817 60-61 0.0038993247353853876 62-63 0.0200644865024685 64-65 0.021275927196957166 66-67 0.01298512994405037 68-69 0.030816022666055397 70-71 0.016449093179853894 72-73 0.005148622951576823 74-75 0.0161840905279345 76-77 0.01974269756799495 78-79 0.02551596962766749 80-81 0.01154654411934508 82-83 0.03452605979292692 84-85 0.02186271878335011 86-87 0.030588877535838773 88-89 0.021938433826755654 90-91 0.027314201908549097 92-93 0.023925953716151117 94-95 0.014329071964498731 96-97 0.014726575942377822 98-99 0.010997610054654904 100-101 0.010051172012085635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2641483.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.75019209860179 #Duplication Level Percentage of deduplicated Percentage of total 1 72.27713136816536 33.06692644429442 2 14.485498953082256 13.254287194952166 3 5.351462213496285 7.344912728275912 4 2.5316577368704656 4.63295311158941 5 1.3942447359056853 3.189348225007471 6 0.8539863779592038 2.344202450473363 7 0.5854908776957903 1.8750424088592959 8 0.4077253355095879 1.4922809938424408 9 0.2883920602424544 1.1874592940223445 >10 1.5996709065742232 13.53843414187462 >50 0.13896272952408673 4.368875611411085 >100 0.0757702621745411 6.443638350076515 >500 0.005044570337222313 1.5613515209681323 >1k 0.004713778839695765 4.414559872491302 >5k 8.269787438062746E-5 0.28947127282446117 >10k+ 1.6539574876125491E-4 0.9962563790372123 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14176 0.5366682276584782 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12156 0.46019603381888124 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7651 0.2896478985478991 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4774 0.18073180860902757 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4541 0.171911006052282 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4476 0.1694502671416019 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4376 0.16566451497132484 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4149 0.15707085754479586 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3541 0.13405348434951125 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3417 0.12935915165836767 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3348 0.12674698266087647 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3319 0.12564911453149613 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3073 0.11633616419261451 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2833 0.10725035898394954 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1357256510831227E-4 2 0.0 0.0 0.0 0.0 1.1357256510831227E-4 3 0.0 0.0 0.0 0.0 1.1357256510831227E-4 4 0.0 0.0 0.0 0.0 1.1357256510831227E-4 5 0.0 0.0 0.0 0.0 1.51430086811083E-4 6 0.0 0.0 0.0 0.0 1.8928760851385376E-4 7 0.0 0.0 0.0 0.0 2.2714513021662453E-4 8 0.0 0.0 0.0 0.0 2.2714513021662453E-4 9 0.0 0.0 0.0 3.785752170277075E-5 2.2714513021662453E-4 10-11 0.0 0.0 0.0 3.785752170277075E-5 3.02860173622166E-4 12-13 0.0 0.0 0.0 3.785752170277075E-5 6.814353906498735E-4 14-15 0.0 0.0 0.0 3.785752170277075E-5 7.760791949068004E-4 16-17 0.0 0.0 0.0 3.785752170277075E-5 8.51794238312342E-4 18-19 0.0 0.0 0.0 7.57150434055415E-5 9.275092817178835E-4 20-21 0.0 0.0 0.0 1.7035884766246839E-4 0.0010221530859748104 22-23 0.0 0.0 0.0 4.353614995818636E-4 0.0010221530859748104 24-25 0.0 0.0 0.0 6.814353906498735E-4 0.0010221530859748104 26-27 0.0 0.0 0.0 0.001211440694488664 0.0010221530859748104 28-29 0.0 0.0 0.0 0.0036911083660201485 0.0010410818468261957 30-31 0.0 0.0 0.0 0.011641187923602008 0.0010600106076775811 32-33 0.0 0.0 0.0 0.028847431537511313 0.0010978681293803518 34-35 0.0 0.0 0.0 0.0534548206443123 0.0011357256510831227 36-37 0.0 0.0 0.0 0.09445451664841303 0.0011357256510831227 38-39 0.0 0.0 0.0 0.1562569208281863 0.001211440694488664 40-41 0.0 0.0 0.0 0.22220472363441296 0.0012492982161914348 42-43 0.0 0.0 0.0 0.29093505428579325 0.0012492982161914348 44-45 0.0 0.0 0.0 0.3745055334446597 0.0012871557378942057 46-47 0.0 0.0 0.0 0.47187507926418604 0.001362870781299747 48-49 0.0 0.0 0.0 0.5710239286037426 0.001457514585556674 50-51 0.0 0.0 0.0 0.6788610791740852 0.00151430086811083 52-53 0.0 0.0 0.0 0.7788617227519541 0.0016657309549219133 54-55 0.0 0.0 0.0 0.88770209764742 0.0016657309549219133 56-57 0.0 0.0 0.0 1.0151305156989463 0.0016846597157732985 58-59 0.0 0.0 0.0 1.1478022005063067 0.001703588476624684 60-61 0.0 0.0 0.0 1.2931940126057975 0.00176037475917884 62-63 0.0 0.0 0.0 1.4335886318405229 0.0019307336068413086 64-65 0.0 0.0 0.0 1.5738318209884372 0.0019496623676926938 66-67 0.0 0.0 0.0 1.7412756394797921 0.001968591128544079 68-69 0.0 0.0 0.0 1.923446033913525 0.001968591128544079 70-71 0.0 0.0 0.0 2.110727193777132 0.0020253774110982354 72-73 0.0 0.0 0.0 2.3052769978076713 0.002214665019612089 74-75 0.0 0.0 0.0 2.4991832239692626 0.002366095106423172 76-77 0.0 0.0 0.0 2.705336358401701 0.0024039526281259428 78-79 0.0 0.0 0.0 2.937573325287348 0.0024985964323828696 80-81 0.0 0.0 0.0 3.1796343190548644 0.0026121689974911823 82-83 0.0 0.0 0.0 3.4340368648974837 0.0027825278451536505 84-85 0.0 0.0 0.0 3.6960109150806577 0.002801456606005036 86-87 0.0 0.0 0.0 3.957473888720844 0.0028771716494105774 88-89 0.0 0.0 0.0 4.236180963496642 0.0029528866928161188 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6000 0.0 59.69638 1 GTATCAA 11300 0.0 44.687157 1 TGGTATC 2420 0.0 35.524952 2 ATCAACG 14535 0.0 34.668064 3 TATCAAC 14630 0.0 34.478672 2 TCAACGC 14795 0.0 34.08899 4 CAACGCA 15000 0.0 33.686424 5 GTGGTAT 2650 0.0 33.521515 1 AACGCAG 15620 0.0 32.470318 6 ACGCAGA 17605 0.0 28.701324 7 CGCAGAG 17665 0.0 28.52319 8 TAAGGTG 2040 0.0 27.004267 5 GTGTAAG 1220 0.0 26.088123 1 GCAGAGT 19310 0.0 25.822802 9 TAAGGTA 975 0.0 24.35398 4 AGGTAAG 1885 0.0 23.93771 2 GTACATG 11290 0.0 23.81499 1 GAGTACT 12325 0.0 23.677252 12-13 TACATGG 11340 0.0 23.455532 2 CAGAGTA 18425 0.0 22.694372 10-11 >>END_MODULE