FastQCFastQC Report
Thu 26 May 2016
SRR938267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031165
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71650.6948451508730417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60150.5833208070483386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36140.35047737268041484No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29830.2892844501122517No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17290.1676744264981841No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16010.1552612821420432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11760.11404576377204423No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11250.10909990156764438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG600.003940020731.6842864
GGTATCA64100.027.3249861
GTATCAA87300.025.9675661
GTACATG53050.024.650011
GAGTACT47500.024.5993612-13
GGACCGA2351.7462298E-1024.2664596
TACATGG53150.023.8749892
GTAAGCG1401.8189894E-1223.77071694-95
AGGACCG3050.023.3736535
ACATGGG53500.022.4751153
GTACTTT53100.022.32086414-15
TATCAAC101050.022.1993832
TCAACGC101050.022.152354
ATCAACG101400.022.0290163
CAACGCA101850.021.9783485
AACGCAG103750.021.711176
AGTACTT49150.020.77768912-13
AGAGTAC78950.020.54669610-11
TACCTGG7650.020.5015962
GTACCTG7450.020.4996071
Produced by FastQC (version 0.11.5)