##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938267_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1031165 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.867903778735702 33.0 31.0 34.0 30.0 34.0 2 32.00950381364767 33.0 31.0 34.0 30.0 34.0 3 32.08451605708107 34.0 31.0 34.0 30.0 34.0 4 35.62667565326596 37.0 35.0 37.0 33.0 37.0 5 35.639690059301856 37.0 35.0 37.0 33.0 37.0 6 35.68579422303899 37.0 35.0 37.0 35.0 37.0 7 35.62488932421097 37.0 35.0 37.0 33.0 37.0 8 35.60184063656156 37.0 35.0 37.0 33.0 37.0 9 37.214513681127656 39.0 37.0 39.0 34.0 39.0 10-11 37.11292373189548 39.0 37.0 39.0 33.5 39.0 12-13 36.968187923368234 39.0 37.0 39.0 33.0 39.0 14-15 38.07723448720621 40.0 38.0 41.0 33.0 41.0 16-17 37.90269258557069 40.0 38.0 41.0 33.0 41.0 18-19 38.12296043795125 40.0 38.0 41.0 33.0 41.0 20-21 38.12534269491303 40.0 38.0 41.0 33.0 41.0 22-23 37.999916599186356 40.0 38.0 41.0 33.0 41.0 24-25 37.84568570500356 40.0 38.0 41.0 32.5 41.0 26-27 37.624107199138834 40.0 37.5 41.0 32.0 41.0 28-29 37.40493277021621 40.0 37.0 41.0 32.0 41.0 30-31 37.134673888272005 39.5 36.5 41.0 31.0 41.0 32-33 36.78282476616255 39.0 36.0 41.0 30.5 41.0 34-35 36.451369567431016 39.0 35.0 41.0 30.0 41.0 36-37 36.122024118351575 39.0 35.0 40.0 30.0 41.0 38-39 35.78608224677913 38.0 35.0 40.0 28.5 41.0 40-41 35.595873599278484 38.0 35.0 40.0 27.0 41.0 42-43 35.288142052920726 38.0 35.0 40.0 26.5 41.0 44-45 34.86862238342069 38.0 34.0 40.0 24.5 41.0 46-47 34.31981254212468 38.0 33.5 40.0 23.5 41.0 48-49 33.77326858456212 38.0 33.0 40.0 19.5 41.0 50-51 32.91001197674475 37.5 32.0 39.5 16.0 40.5 52-53 32.88531951724506 37.5 32.0 39.5 14.5 40.5 54-55 32.83883908006963 37.5 32.0 40.0 11.0 41.0 56-57 32.34008718294356 37.0 31.0 40.0 7.5 41.0 58-59 31.85661606047529 36.5 31.0 40.0 2.0 41.0 60-61 31.247608287713412 36.0 30.0 39.0 2.0 41.0 62-63 30.60261791274917 35.0 29.0 39.0 2.0 40.5 64-65 29.975458340808697 35.0 28.0 38.5 2.0 40.0 66-67 29.253645148933487 34.5 27.0 38.0 2.0 40.0 68-69 28.524655123088934 34.0 26.0 37.5 2.0 40.0 70-71 27.654323992765462 34.0 24.0 37.0 2.0 39.0 72-73 26.829056940450847 33.5 21.5 36.0 2.0 39.0 74-75 25.975391911090853 32.5 19.0 35.5 2.0 38.5 76-77 25.11889367850926 32.0 10.5 35.0 2.0 37.5 78-79 24.256430348198396 31.0 4.5 35.0 2.0 37.0 80-81 23.41565171432312 31.0 2.0 35.0 2.0 36.0 82-83 22.575209593033122 30.0 2.0 34.0 2.0 36.0 84-85 21.742677941939455 29.0 2.0 34.0 2.0 35.0 86-87 20.97000577017257 29.0 2.0 34.0 2.0 35.0 88-89 20.129045788016466 27.5 2.0 34.0 2.0 35.0 90-91 19.257948533939768 26.0 2.0 34.0 2.0 35.0 92-93 18.34620938453109 24.5 2.0 33.0 2.0 35.0 94-95 17.406532902105873 21.0 2.0 33.0 2.0 35.0 96-97 16.311067578903476 8.5 2.0 32.0 2.0 34.5 98-99 15.031215663836534 2.0 2.0 32.0 2.0 34.0 100-101 12.144079269564038 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4091056422120296 1101 2 0.4763798524614131 1101 3 0.4598381805274485 1101 4 0.2517986496984932 1101 5 0.3486305804807799 1101 6 0.39149297840895514 1101 7 0.46459643098029346 1101 8 0.3357456355608761 1101 9 0.49637894838973295 1101 10-11 0.3232044956404181 1101 12-13 0.5352229425975068 1101 14-15 0.6633830235405398 1101 16-17 0.6241618184660993 1101 18-19 0.6967879935857155 1101 20-21 0.6470357831185609 1101 22-23 0.7394631019444873 1101 24-25 0.7400571690493507 1101 26-27 0.9920260945954738 1101 28-29 1.0485354660942647 1101 30-31 1.1188361569221357 1101 32-33 1.1814330252749201 1101 34-35 1.0696651459892408 1101 36-37 1.1869988981396702 1101 38-39 1.4452425247445504 1101 40-41 1.5834831530430904 1101 42-43 1.7429184098406552 1101 44-45 1.6687955963223757 1101 46-47 1.94457512395595 1101 48-49 2.030692299493289 1101 50-51 2.4177472359741046 1101 52-53 2.5150069421836037 1101 54-55 2.6483828750065683 1101 56-57 2.699855529873588 1101 58-59 3.029565784258022 1101 60-61 3.079377434257321 1101 62-63 3.271783161885022 1101 64-65 3.338497772918071 1101 66-67 3.5528889184299004 1101 68-69 3.8387101394118055 1101 70-71 3.8965662057813724 1101 72-73 4.003646912352444 1101 74-75 4.283208365452207 1101 76-77 4.163376248345159 1101 78-79 4.467215582673063 1101 80-81 4.637110280386526 1101 82-83 4.867364709997208 1101 84-85 5.091735877879692 1101 86-87 4.8144385458945 1101 88-89 4.984096421448829 1101 90-91 4.997732785950397 1101 92-93 5.585104580486281 1101 94-95 5.959316768784948 1101 96-97 5.985168883407464 1101 98-99 5.654831149149812 1101 100-101 4.26638136958435 1102 1 0.5076127199246763 1102 2 0.47194539708484484 1102 3 0.4660285611873647 1102 4 0.3473659579631345 1102 5 0.30609265668330465 1102 6 0.27870040686574526 1102 7 0.4122405370015869 1102 8 0.41058354591059043 1102 9 0.5281586607839444 1102 10-11 0.6330542113634934 1102 12-13 0.5787606305079649 1102 14-15 0.881086343815582 1102 16-17 0.7952647776356017 1102 18-19 0.5025433060037372 1102 20-21 0.6420165704520926 1102 22-23 0.7406877303122172 1102 24-25 0.716826707438301 1102 26-27 0.8339366030311979 1102 28-29 0.7372037406138361 1102 30-31 0.8284680629553307 1102 32-33 0.8607165239863619 1102 34-35 0.8283466698634285 1102 36-37 1.0176744609065693 1102 38-39 0.9430396266411165 1102 40-41 1.0255304539004442 1102 42-43 1.1804788972229048 1102 44-45 1.4582350229626186 1102 46-47 1.6623341091567951 1102 48-49 1.8645732801659918 1102 50-51 1.9492728738059668 1102 52-53 2.1065090574507863 1102 54-55 2.332514733641908 1102 56-57 2.6856907352385804 1102 58-59 2.7080661160430246 1102 60-61 2.7552455315309494 1102 62-63 3.0469530776931997 1102 64-65 3.0011666691211722 1102 66-67 3.365984836261859 1102 68-69 3.55381429344823 1102 70-71 3.885092617410823 1102 72-73 4.0921712296579535 1102 74-75 4.365115721379254 1102 76-77 4.562151509749857 1102 78-79 4.715814590183967 1102 80-81 4.8841176017021155 1102 82-83 5.088501619260068 1102 84-85 5.07830685231351 1102 86-87 5.520524981950384 1102 88-89 5.531099664344108 1102 90-91 5.674580806373363 1102 92-93 5.550724048439818 1102 94-95 5.690241335645691 1102 96-97 5.638225383858295 1102 98-99 5.33606843164325 1102 100-101 4.2483299506247665 1103 1 0.42795532961303095 1103 2 0.20335840694156815 1103 3 0.1397393901918349 1103 4 0.14929074582510538 1103 5 0.24420139422953469 1103 6 0.1782974429390407 1103 7 0.08003392949865429 1103 8 0.13194531100100448 1103 9 0.32957004432886094 1103 10-11 0.29133793155708076 1103 12-13 0.3541159477503797 1103 14-15 0.7151913780478552 1103 16-17 0.780522739388573 1103 18-19 0.6208197556268615 1103 20-21 0.5295363811621527 1103 22-23 0.6248316153266558 1103 24-25 0.7217688883330737 1103 26-27 0.7174809848679473 1103 28-29 0.8483982932133358 1103 30-31 0.8198316031704138 1103 32-33 0.8696750572869618 1103 34-35 0.8914831769495777 1103 36-37 1.032103992177987 1103 38-39 1.1186573859661095 1103 40-41 1.1956777467290465 1103 42-43 1.307273287828984 1103 44-45 1.443517964014653 1103 46-47 1.8590721315828844 1103 48-49 1.9633132605439485 1103 50-51 2.1471174260334323 1103 52-53 2.2365837083517945 1103 54-55 2.39733495178028 1103 56-57 2.6676716255433206 1103 58-59 2.707630838469864 1103 60-61 2.7845850469750637 1103 62-63 2.9116779493155427 1103 64-65 3.19362844813622 1103 66-67 3.5281546976214315 1103 68-69 3.639891895095378 1103 70-71 3.891774314174885 1103 72-73 4.128270037135586 1103 74-75 4.308553021744167 1103 76-77 4.583896255327723 1103 78-79 4.695885361321505 1103 80-81 4.91640148558983 1103 82-83 5.009602642986152 1103 84-85 5.228194339172802 1103 86-87 5.556796518623717 1103 88-89 5.764239434683372 1103 90-91 5.869284876085807 1103 92-93 5.768004089767267 1103 94-95 5.834874134634614 1103 96-97 5.221806650021758 1103 98-99 5.058754099584393 1103 100-101 4.019388828427099 1104 1 0.5101442095746656 1104 2 0.41903259118765845 1104 3 0.41140615028326977 1104 4 0.4275283810574564 1104 5 0.3634684873283476 1104 6 0.32608088848589745 1104 7 0.46729618942296014 1104 8 0.4091988720416353 1104 9 0.5825580797306174 1104 10-11 0.5668792242651719 1104 12-13 0.6562158250525201 1104 14-15 0.7800593065716797 1104 16-17 0.8485593245265619 1104 18-19 0.8461218809364723 1104 20-21 0.8363837936075811 1104 22-23 0.8410829570100873 1104 24-25 0.8683269123785351 1104 26-27 1.0058219894125457 1104 28-29 1.124603420779664 1104 30-31 1.0408424176059867 1104 32-33 1.2020019982902568 1104 34-35 1.2195727858319927 1104 36-37 1.2977417407657228 1104 38-39 1.3784202739124467 1104 40-41 1.563487932519763 1104 42-43 1.8010288803392243 1104 44-45 1.7002938546121769 1104 46-47 1.6984848219877549 1104 48-49 2.085243997499262 1104 50-51 2.1979752729870583 1104 52-53 2.3403256816073963 1104 54-55 2.470407650780217 1104 56-57 2.6106321733031024 1104 58-59 2.6296398446172127 1104 60-61 3.0136693181892333 1104 62-63 3.0007480822486023 1104 64-65 3.1959692575268654 1104 66-67 3.2890101362922266 1104 68-69 3.3820483670440744 1104 70-71 3.6661405309494093 1104 72-73 4.0111444472620015 1104 74-75 4.1315374862437935 1104 76-77 4.567364743154478 1104 78-79 4.4107477881647235 1104 80-81 4.764807347682417 1104 82-83 4.779359904685528 1104 84-85 5.015860609303246 1104 86-87 4.809262932420683 1104 88-89 5.031115061407835 1104 90-91 5.366564139317347 1104 92-93 5.234763558367206 1104 94-95 5.039799280636757 1104 96-97 5.123475527741199 1104 98-99 4.875341562811366 1104 100-101 3.5614849894996983 1105 1 0.5558330780499467 1105 2 0.5245863672678865 1105 3 0.5396432005712413 1105 4 0.5586683263839163 1105 5 0.32294379056101974 1105 6 0.3293363210897837 1105 7 0.48730017813370097 1105 8 0.31976915695242525 1105 9 0.5264320848192412 1105 10-11 0.5123532462756017 1105 12-13 0.5725752804122024 1105 14-15 0.8017484248450089 1105 16-17 0.8461585437062169 1105 18-19 0.6068737730559448 1105 20-21 0.7152186468814463 1105 22-23 0.6938370811959729 1105 24-25 0.6640036938515763 1105 26-27 0.7817994753563724 1105 28-29 0.8592816345624712 1105 30-31 0.7985443561596952 1105 32-33 0.9264027347915871 1105 34-35 1.0814138175785004 1105 36-37 1.0144858649717534 1105 38-39 1.2453781366081245 1105 40-41 1.3417589271390256 1105 42-43 1.5016746284340101 1105 44-45 1.49655232306813 1105 46-47 1.5841936541617443 1105 48-49 1.514826129686746 1105 50-51 1.5776536982924085 1105 52-53 1.718026371170879 1105 54-55 1.9039835299585448 1105 56-57 2.27115108845436 1105 58-59 2.4508408494499996 1105 60-61 2.307653919348379 1105 62-63 2.5892309821621815 1105 64-65 2.5951717168289186 1105 66-67 2.6056039787703824 1105 68-69 2.639822323986145 1105 70-71 3.2269164963483483 1105 72-73 3.6307231749438564 1105 74-75 3.549524657905412 1105 76-77 3.698121429784841 1105 78-79 3.6799082048439793 1105 80-81 3.959464489791319 1105 82-83 4.124883483405153 1105 84-85 4.053761419333846 1105 86-87 4.25923029751463 1105 88-89 4.449853641708842 1105 90-91 4.581794366492552 1105 92-93 4.590193946299543 1105 94-95 4.5654327906417755 1105 96-97 4.41287008979889 1105 98-99 4.67415139599059 1105 100-101 4.035365984904621 1106 1 0.4917043430068695 1106 2 0.4272032149172489 1106 3 0.43269014541446893 1106 4 0.35411468726796613 1106 5 0.26733274464703527 1106 6 0.4007577511102909 1106 7 0.21357513787487648 1106 8 0.3615762077547373 1106 9 0.4900229678342427 1106 10-11 0.44747029534040195 1106 12-13 0.45486081664805056 1106 14-15 0.5460173062079221 1106 16-17 0.5845985107420333 1106 18-19 0.7031299696141176 1106 20-21 0.8002115949143231 1106 22-23 0.655386145512054 1106 24-25 0.6883628287455679 1106 26-27 0.8703101561987978 1106 28-29 0.9341397458777436 1106 30-31 1.03302056438541 1106 32-33 0.9095299449013865 1106 34-35 1.0489825342175507 1106 36-37 1.0615821337871054 1106 38-39 1.254390385578965 1106 40-41 1.380864099002217 1106 42-43 1.3332631701765152 1106 44-45 1.6357153472700148 1106 46-47 1.5529131081754386 1106 48-49 1.7949789918729593 1106 50-51 1.9251462944549331 1106 52-53 1.9826440361301252 1106 54-55 2.050211140084251 1106 56-57 1.9454103892370114 1106 58-59 2.227442490476246 1106 60-61 2.690874217365579 1106 62-63 2.469323759453001 1106 64-65 2.9530490989911833 1106 66-67 2.8977146087493537 1106 68-69 2.881119478252902 1106 70-71 3.206052768696967 1106 72-73 3.422520761647135 1106 74-75 3.5573148247550535 1106 76-77 3.5476941863808342 1106 78-79 4.270768894276902 1106 80-81 4.664304478570298 1106 82-83 4.395531963470848 1106 84-85 4.2336453069721784 1106 86-87 4.331121049779462 1106 88-89 4.6103933962635 1106 90-91 4.800563567693473 1106 92-93 4.828942588565333 1106 94-95 4.562535320322642 1106 96-97 4.66358863331773 1106 98-99 4.64244181084816 1106 100-101 3.4142468721808914 1107 1 0.5121829266846625 1107 2 0.5499527911506306 1107 3 0.4175079009878715 1107 4 0.2897596551013564 1107 5 0.3170120697925043 1107 6 0.23194400025532502 1107 7 0.25238125597442007 1107 8 0.273730131684907 1107 9 0.3658410108750516 1107 10-11 0.329958077975391 1107 12-13 0.4636702059284872 1107 14-15 0.6892345621827829 1107 16-17 0.8252776277214409 1107 18-19 0.6841734763200336 1107 20-21 0.6794529783264593 1107 22-23 0.6897097545167199 1107 24-25 0.823566682791423 1107 26-27 0.8491555284690975 1107 28-29 1.0187022502145382 1107 30-31 1.167097843555915 1107 32-33 1.0990745568699438 1107 34-35 1.4431558671543812 1107 36-37 1.5040296709844014 1107 38-39 1.3239951674506756 1107 40-41 1.5150781730102096 1107 42-43 1.4439520015719225 1107 44-45 1.5656243489650805 1107 46-47 1.9133442101668905 1107 48-49 1.8614181627772908 1107 50-51 2.0031594981305503 1107 52-53 2.2702923544457576 1107 54-55 2.310785975781762 1107 56-57 2.786242656958393 1107 58-59 2.7512829559751957 1107 60-61 2.8322080661577154 1107 62-63 2.7644070754391628 1107 64-65 3.090788118231668 1107 66-67 3.26193284607848 1107 68-69 3.415325126491915 1107 70-71 3.651722419077121 1107 72-73 3.5952842156176565 1107 74-75 3.903539584620635 1107 76-77 4.076213927461907 1107 78-79 4.136748117321872 1107 80-81 4.052473212028527 1107 82-83 4.4970922176316925 1107 84-85 4.710411001205976 1107 86-87 4.793461331894033 1107 88-89 4.843175585179154 1107 90-91 4.349036520975066 1107 92-93 4.511155790258421 1107 94-95 4.869852357227511 1107 96-97 5.029331439197925 1107 98-99 4.933501574331869 1107 100-101 4.303273547174202 1108 1 0.44278697344821083 1108 2 0.41303587805323616 1108 3 0.4411567716461846 1108 4 0.22544812426306748 1108 5 0.2501111614435345 1108 6 0.15683509377181082 1108 7 0.3296004574328677 1108 8 0.27112273558328326 1108 9 0.306318185559995 1108 10-11 0.33458726597289257 1108 12-13 0.5098995507937261 1108 14-15 0.6925171254863258 1108 16-17 0.7141702263202347 1108 18-19 0.6361322031476035 1108 20-21 0.5263082042477905 1108 22-23 0.5639219711213812 1108 24-25 0.6706047444150016 1108 26-27 0.7665636071573445 1108 28-29 0.8499342978684155 1108 30-31 0.6772152823722024 1108 32-33 0.6916461277730974 1108 34-35 0.9768432128039493 1108 36-37 1.0945640811935178 1108 38-39 1.0562855423433604 1108 40-41 1.1318699098963805 1108 42-43 0.9842892671977452 1108 44-45 1.15620433561012 1108 46-47 1.2384096600487169 1108 48-49 1.2696012528596512 1108 50-51 1.6189040369076153 1108 52-53 1.7119359679151813 1108 54-55 1.9191534914420387 1108 56-57 2.0877731772717 1108 58-59 2.058565116510241 1108 60-61 2.383849347809498 1108 62-63 2.3586114486571823 1108 64-65 2.6701626611424167 1108 66-67 2.72702023624457 1108 68-69 2.78487158795863 1108 70-71 3.129997297981589 1108 72-73 3.2263657550412717 1108 74-75 3.534911011375062 1108 76-77 3.630119192070218 1108 78-79 3.6315020575637504 1108 80-81 3.8127628873759143 1108 82-83 4.095388930828719 1108 84-85 3.912169403698904 1108 86-87 4.101585066617975 1108 88-89 4.373355665096906 1108 90-91 4.457227560435779 1108 92-93 4.7338820030685795 1108 94-95 4.644295105804574 1108 96-97 4.567255273554078 1108 98-99 4.615009200435507 1108 100-101 3.3887738810480936 1109 1 0.2186129720085539 1109 2 0.0917498878986649 1109 3 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0.2577024088707134 2311 44-45 0.3949335702097798 2311 46-47 0.7653807041388703 2311 48-49 0.9210711442044683 2311 50-51 1.0541288692612127 2311 52-53 1.0466853163225949 2311 54-55 1.1829511761727716 2311 56-57 1.434499418118378 2311 58-59 1.5142920794681878 2311 60-61 1.4084218165650206 2311 62-63 1.6410932924479589 2311 64-65 1.7409591837253195 2311 66-67 1.7863137519110026 2311 68-69 2.04686634512991 2311 70-71 2.487857968115893 2311 72-73 2.705026802779596 2311 74-75 2.841304328836994 2311 76-77 2.9028186847118285 2311 78-79 2.952885295555827 2311 80-81 3.2126964117678583 2311 82-83 3.731126104891384 2311 84-85 4.291292395797051 2311 86-87 4.219406265097032 2311 88-89 4.157789465922068 2311 90-91 4.482467606019053 2311 92-93 4.718544190388755 2311 94-95 4.4078370427928455 2311 96-97 3.9944259325182223 2311 98-99 3.676137612129194 2311 100-101 3.5137329802142805 2312 1 -0.22221367086982724 2312 2 -0.07915406690359461 2312 3 -0.1826250070397748 2312 4 -0.08977250864133168 2312 5 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2.0303007354990044 2312 68-69 2.3695648925737878 2312 70-71 2.8843114674556425 2312 72-73 2.9710285005724657 2312 74-75 2.617893651606284 2312 76-77 2.782846038041388 2312 78-79 3.4166657143447345 2312 80-81 3.5645545517414483 2312 82-83 3.9287774914222275 2312 84-85 4.077875116038513 2312 86-87 4.373990305844057 2312 88-89 4.68084172021371 2312 90-91 4.703661720337106 2312 92-93 4.661328570694359 2312 94-95 5.042420140321614 2312 96-97 4.944855096826846 2312 98-99 4.762116672683808 2312 100-101 4.212204966631939 2313 1 -0.38572063725663597 2313 2 -0.2858962654531432 2313 3 -0.21900820345276273 2313 4 -0.22426740798231037 2313 5 -0.2804531484274335 2313 6 -0.20607772644564903 2313 7 -0.11556233083709344 2313 8 4.3110366890175555E-4 2313 9 -0.09552739011598987 2313 10-11 0.006678108469010624 2313 12-13 0.008047945589247263 2313 14-15 -0.06091790589421464 2313 16-17 -0.06621274773119268 2313 18-19 -0.1642256088714973 2313 20-21 0.02436435625670441 2313 22-23 0.038537656174817414 2313 24-25 0.17734026769707611 2313 26-27 0.0565978764774826 2313 28-29 0.10541846900623142 2313 30-31 0.1539093548957311 2313 32-33 0.28377057364142644 2313 34-35 0.4335408490487964 2313 36-37 0.5946338257394999 2313 38-39 0.6556418226822345 2313 40-41 0.7862235894386345 2313 42-43 0.870376625166351 2313 44-45 1.0622795805259742 2313 46-47 1.1872054642168308 2313 48-49 1.263619420638932 2313 50-51 1.2400377142540435 2313 52-53 1.0876165396944728 2313 54-55 1.5119294863592287 2313 56-57 1.7376911036898548 2313 58-59 1.9052090089578506 2313 60-61 2.0755280585343137 2313 62-63 2.276490170541127 2313 64-65 2.450708072491249 2313 66-67 2.5706997605057715 2313 68-69 2.418679424185836 2313 70-71 2.828404332551486 2313 72-73 3.3909579339328815 2313 74-75 3.610943815609776 2313 76-77 3.8019238569454217 2313 78-79 3.9103425522370614 2313 80-81 3.97334228006741 2313 82-83 4.210976341893172 2313 84-85 4.180022207609639 2313 86-87 4.230101316647225 2313 88-89 4.522640041058704 2313 90-91 4.5521525872986786 2313 92-93 4.535321980462346 2313 94-95 4.522053615089149 2313 96-97 4.6250528037872165 2313 98-99 4.729804223464702 2313 100-101 3.9337195162994174 2314 1 -0.4541380648297917 2314 2 -0.40957976571640486 2314 3 -0.33543685178491245 2314 4 -0.23958017484036276 2314 5 -0.20988979494255489 2314 6 -0.24780013064959405 2314 7 -0.22773894865137123 2314 8 -0.12010503859050203 2314 9 -0.20952398908151793 2314 10-11 -0.24061219917270193 2314 12-13 -0.19102724146026873 2314 14-15 -0.058707454718820884 2314 16-17 -0.07350078408362037 2314 18-19 0.07730904525096349 2314 20-21 -0.16436134721615758 2314 22-23 -0.3263250561870521 2314 24-25 -0.2533503812279676 2314 26-27 -0.11101626687037225 2314 28-29 -0.2982180273678239 2314 30-31 -0.15313761507163548 2314 32-33 -0.0703075705087457 2314 34-35 0.15759431873814123 2314 36-37 0.16997241745165326 2314 38-39 0.2457961523642993 2314 40-41 0.3857101158261571 2314 42-43 0.6322728714920984 2314 44-45 0.6640805359423325 2314 46-47 0.6373985975488878 2314 48-49 0.6790905878013973 2314 50-51 0.8208215007882558 2314 52-53 1.022207464271446 2314 54-55 1.0274682759980251 2314 56-57 1.2839063098241041 2314 58-59 1.6703458983829371 2314 60-61 1.8407821995813798 2314 62-63 1.8334066151179762 2314 64-65 2.261864748680619 2314 66-67 2.2157105196325944 2314 68-69 2.462482860322048 2314 70-71 2.973311482540648 2314 72-73 3.3396596118847093 2314 74-75 3.487465690042871 2314 76-77 3.5735110604426197 2314 78-79 3.5450447552401805 2314 80-81 3.8325573726371935 2314 82-83 4.325679031601137 2314 84-85 4.573307376828062 2314 86-87 4.9938621587351015 2314 88-89 4.979269108546969 2314 90-91 4.872069950715286 2314 92-93 5.152887379582442 2314 94-95 5.223130191459777 2314 96-97 5.057893065781908 2314 98-99 4.9979439343030325 2314 100-101 4.918868012897645 2315 1 -0.44274926678657067 2315 2 -0.5612677263671166 2315 3 -0.4411848196695871 2315 4 -0.23596102779816874 2315 5 -0.15608413151528566 2315 6 -0.15679731720135948 2315 7 -0.23044193818378744 2315 8 -0.190280249202047 2315 9 -0.393190106342459 2315 10-11 -0.4499505803967381 2315 12-13 -0.29908581778470733 2315 14-15 -0.4052416864496351 2315 16-17 -0.3806824771051609 2315 18-19 -0.2504612843852527 2315 20-21 -0.34151870322457256 2315 22-23 -0.30308468865555227 2315 24-25 -0.18612723370350182 2315 26-27 -0.07749638105435253 2315 28-29 -0.10414460625952415 2315 30-31 -0.2541817025347157 2315 32-33 -0.2827499857858342 2315 34-35 0.059945934070391615 2315 36-37 -0.09777299039004816 2315 38-39 0.1541760696718839 2315 40-41 0.16907532335496 2315 42-43 0.3222004813317412 2315 44-45 0.6012208683147477 2315 46-47 0.7236271402231225 2315 48-49 0.8961903175746428 2315 50-51 0.9232762831087911 2315 52-53 1.1133005217612322 2315 54-55 1.2357497190074653 2315 56-57 1.551235151170033 2315 58-59 1.8937746232779453 2315 60-61 1.9535677588020484 2315 62-63 2.1820749379432947 2315 64-65 2.430170608117944 2315 66-67 2.2646018535333816 2315 68-69 2.7700410514849203 2315 70-71 3.1837988509016633 2315 72-73 3.5739970365219094 2315 74-75 3.8424373786821597 2315 76-77 3.6681712844066716 2315 78-79 4.0803771023866275 2315 80-81 4.596717926172488 2315 82-83 4.546833967625329 2315 84-85 4.684565645889592 2315 86-87 4.936833339119943 2315 88-89 5.054408824372093 2315 90-91 4.772668304808359 2315 92-93 5.1283211465925405 2315 94-95 5.175481698499173 2315 96-97 5.245027442047579 2315 98-99 5.18567825295691 2315 100-101 4.878389950502191 2316 1 -0.6466458351076874 2316 2 -0.6218661604312352 2316 3 -0.447600421868124 2316 4 -0.5832481537235594 2316 5 -0.4187477297268245 2316 6 -0.36813041023398796 2316 7 -0.24316010171765612 2316 8 -0.2519671459175328 2316 9 -0.240412891399167 2316 10-11 -0.3807112743112313 2316 12-13 -0.27296626889596354 2316 14-15 -0.30084489453511765 2316 16-17 -0.16157267034815703 2316 18-19 -0.33994450279370625 2316 20-21 -0.4236119640695577 2316 22-23 -0.3880697338470114 2316 24-25 -0.5530017107117757 2316 26-27 -0.3865213076311065 2316 28-29 -0.35847126307243116 2316 30-31 -0.4595723298119765 2316 32-33 -0.14852035926858065 2316 34-35 -0.2259603586087522 2316 36-37 0.08693364724147301 2316 38-39 0.032231638853957634 2316 40-41 0.16343429990146774 2316 42-43 0.295078850073871 2316 44-45 0.5474848677858049 2316 46-47 0.5755432539493412 2316 48-49 0.8368922089117987 2316 50-51 0.6356704672630258 2316 52-53 0.7509135537375755 2316 54-55 0.96844255854419 2316 56-57 1.1384180946545683 2316 58-59 1.209382580501579 2316 60-61 1.299107372515106 2316 62-63 1.787523934119104 2316 64-65 1.9203303654284518 2316 66-67 2.3266374706476256 2316 68-69 2.3477929033288696 2316 70-71 2.7889165449249305 2316 72-73 3.0355809167526253 2316 74-75 3.2324724355687273 2316 76-77 3.385514383333067 2316 78-79 3.9321094358415323 2316 80-81 4.174582107930821 2316 82-83 4.359159118648634 2316 84-85 4.302839092448636 2316 86-87 4.718865671650615 2316 88-89 4.856850009353156 2316 90-91 5.156454476995901 2316 92-93 5.424278122813433 2316 94-95 5.494737534144864 2316 96-97 5.520932462128332 2316 98-99 5.465001159514772 2316 100-101 4.992027299772156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2751.0 3 951.0 4 989.0 5 1148.0 6 1388.0 7 1812.0 8 2271.0 9 2993.0 10 3764.0 11 4731.0 12 5998.0 13 7749.0 14 9175.0 15 11259.0 16 14013.0 17 17323.0 18 17350.0 19 15482.0 20 16444.0 21 17929.0 22 19939.0 23 22221.0 24 24966.0 25 26563.0 26 29149.0 27 31336.0 28 34660.0 29 37302.0 30 41024.0 31 45185.0 32 50994.0 33 58230.0 34 71055.0 35 89257.0 36 105670.0 37 117438.0 38 66412.0 39 4244.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.744596819178774 18.574617486631382 14.471500235100793 22.209285459089052 2 15.49392092841886 20.216235301877752 40.536358997937754 23.75348477176564 3 19.87658932478255 24.788858111680838 29.512615151765054 25.821937411771557 4 12.706314011550576 16.862426280910114 36.85658599242703 33.57467371511228 5 13.590526853068411 37.55586909311095 33.53242105549162 15.321182998329022 6 31.28865669266583 35.956570594616835 17.446382791258227 15.30838992145911 7 28.30262991957937 33.39735940940795 20.205950544706695 18.094060126305987 8 26.522087511907603 34.12849928311866 19.346871622945635 20.002541582028105 9 26.6021866299318 17.26574247436482 20.393865018771645 35.73820587693174 10-11 25.3421391780705 26.60801169542422 26.37548074101416 21.674368385491118 12-13 26.536529763420518 24.302860538154654 27.178629027368522 21.9819806710563 14-15 23.06067513822873 25.419390823552234 26.489863226307108 25.03007081191192 16-17 21.85545428397229 28.827267951804192 26.84674048697344 22.470537277250077 18-19 21.548440229743456 27.085990829274536 29.87882303526374 21.48674590571827 20-21 22.649840894097558 25.778842795607126 30.05951918972409 21.51179712057123 22-23 22.26698871380001 25.834097225253537 30.137209539191296 21.761704521755156 24-25 22.210999841366633 26.062540355697728 30.15290413062851 21.573555672307126 26-27 21.776079531480644 26.198160247551144 30.30236146677643 21.723398754191784 28-29 21.440884561022624 26.75369286571424 30.261418126399953 21.544004446863184 30-31 21.717390249818095 26.138200339558576 30.139752607324766 22.00465680329857 32-33 21.54816434215166 26.297101684778205 30.07920635513493 22.075527617935204 34-35 22.304553217723928 26.280888068812676 29.769979292752176 21.644579420711224 36-37 22.14001985856472 25.968250549700013 30.0372675507463 21.85446204098897 38-39 21.73650101287567 26.37337162716282 30.028114150142265 21.862013209819246 40-41 21.90797829545934 26.376865430974995 29.86731210630938 21.847844167256287 42-43 21.827418798710227 26.355448198336912 29.482407700622726 22.33472530233014 44-45 21.908438222107993 26.403020214780526 29.485549959871104 22.202991603240378 46-47 22.26966864292729 26.88034530001576 28.918051867748158 21.931934189308794 48-49 22.265496939893893 26.7026992948662 28.89841029669935 22.133393468540557 50-51 21.88681587636735 27.097549159622503 28.905746495456835 22.10988846855331 52-53 22.308358500191055 27.207215856963025 28.37274686311363 22.11167877973229 54-55 21.917402356081112 26.915807001907638 28.705998046782184 22.46079259522907 56-57 22.276022832580313 27.061534589535828 28.519533824311722 22.142908753572137 58-59 22.59208110721355 26.661407059640453 28.41768585695873 22.328825976187268 60-61 22.064689572101496 26.847792037505247 29.083734115403896 22.003784274989354 62-63 21.916810485077395 26.98128752351243 28.94379662177463 22.158105369635543 64-65 21.76957771804517 27.118550369443973 28.699907329863493 22.411964582647357 66-67 21.962825516760812 27.03027464078777 28.330329290604645 22.67657055184677 68-69 21.884360375266038 26.901291183226594 28.599996993526872 22.6143514479805 70-71 22.14007895323912 26.982899931134153 28.383786457676603 22.493234657950126 72-73 22.240763668335397 26.733677748373342 28.50245411477042 22.523104468520845 74-75 21.949880608074423 27.50480574322686 28.04139049403622 22.50392315466249 76-77 22.19316983282214 26.967352431292202 28.100573423691007 22.73890431219465 78-79 22.39871358936112 27.094490663242443 28.01740107032779 22.489394677068645 80-81 22.425058579808198 27.203968297073338 27.908429285248747 22.46254383786971 82-83 22.400202124734435 27.053144547796826 27.985983173406826 22.560670154061917 84-85 22.46919459087517 26.98942255890378 27.921525308417777 22.61985754180327 86-87 22.529324969294304 26.933392037418034 27.848089603897748 22.689193389389917 88-89 22.502074602260794 26.917837020410403 27.616872494047794 22.96321588328101 90-91 22.615933320335728 26.877402496122542 27.634610105736503 22.872054077805227 92-93 22.83172030313361 26.647174841093452 27.67464202077436 22.846462834998576 94-95 22.690085594778953 26.477004648347467 27.715965793129136 23.11694396374444 96-97 22.674908096258864 26.457753377886846 27.763174486163518 23.10416403969078 98-99 22.714372455106083 26.501863264874597 27.23038916144351 23.553375118575808 100-101 23.509448818515402 25.428581553432462 27.532714916992436 23.529254711059696 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1268.0 1 862.0 2 737.5 3 986.5 4 720.5 5 520.0 6 934.5 7 1995.0 8 3216.0 9 3669.0 10 2876.0 11 1967.0 12 1787.0 13 1844.0 14 1886.5 15 1947.5 16 1997.0 17 2040.5 18 2111.0 19 2238.0 20 2461.5 21 2772.5 22 3173.5 23 3728.0 24 4432.0 25 5216.5 26 6199.0 27 7496.5 28 8592.5 29 9779.0 30 11638.0 31 13225.0 32 14780.5 33 16232.0 34 17446.0 35 18777.5 36 20419.5 37 22357.0 38 24289.5 39 25617.0 40 26884.0 41 28938.0 42 30811.0 43 32534.5 44 34349.0 45 36050.0 46 36821.0 47 38594.0 48 42399.5 49 43009.0 50 40261.0 51 37679.0 52 35786.0 53 34924.5 54 33715.5 55 31262.0 56 28549.0 57 26274.5 58 24309.0 59 21971.5 60 19074.5 61 15861.5 62 12709.5 63 9923.5 64 7779.5 65 6249.0 66 4894.5 67 3834.0 68 3019.0 69 2276.5 70 1693.5 71 1271.0 72 983.0 73 783.0 74 609.5 75 492.5 76 352.0 77 225.5 78 145.0 79 91.0 80 68.5 81 40.5 82 17.0 83 12.5 84 9.5 85 5.0 86 2.5 87 2.5 88 2.0 89 3.5 90 4.5 91 2.0 92 0.5 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38267396585415525 2 0.024341400260869984 3 0.044609737529881247 4 0.03820920997124612 5 0.06119292256816319 6 0.06002919028477499 7 0.03248752624458743 8 0.03016006167781102 9 0.03442708005023445 10-11 0.03243903739944626 12-13 0.0317601935674698 14-15 0.025699087924822894 16-17 0.031857171257752157 18-19 0.02676584251792875 20-21 0.03927596456435197 22-23 0.025214199473411143 24-25 0.047179646322363544 26-27 0.041554940285987205 28-29 0.03161472703204628 30-31 0.04024574146717548 32-33 0.03651210039130498 34-35 0.03554232348848147 36-37 0.03641512270102263 38-39 0.018377272308505426 40-41 0.013237454723540851 42-43 0.012752566272129095 44-45 0.010909990156764436 46-47 0.017213540025117224 48-49 0.015031541993764335 50-51 0.010716034776199736 52-53 0.004557951443270476 54-55 0.004460973752988125 56-57 0.009164391731682126 58-59 0.0034911968501646195 60-61 0.0054307506558116305 62-63 0.005964127952364558 64-65 0.008582525589988024 66-67 0.005285284120388105 68-69 0.005479239500952806 70-71 0.016971095799411345 72-73 0.013722343174952604 74-75 0.010376612860211508 76-77 0.007515770996882168 78-79 0.007661237532305693 80-81 0.0090189251962586 82-83 0.011491856298458539 84-85 0.005382261810670455 86-87 4.3639960627057744E-4 88-89 0.023711045274034708 90-91 0.01997740419816421 92-93 0.01304349934297615 94-95 0.025311177163693493 96-97 0.018910649605058356 98-99 0.017359006560540746 100-101 0.1133184310949266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1031165.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.90412828135528 #Duplication Level Percentage of deduplicated Percentage of total 1 84.16870848446769 53.78727944267418 2 9.446440577855487 12.073331009789538 3 2.5815275166211062 4.94910796752011 4 1.1495584397223626 2.9384611999572994 5 0.6447214798897266 2.060018207830915 6 0.39419547573654423 1.5114430949638797 7 0.28004795586919196 1.2527354347757313 8 0.2006253231689539 1.025662911062175 9 0.15420692339352138 0.8869013112971444 >10 0.890451951772212 10.416935607647288 >50 0.058515361341249586 2.5351540308310114 >100 0.027946273334072222 3.247084928453084 >500 0.0016798302553856356 0.7379693113155608 >1k 0.0010689828897908588 1.2916902677298019 >5k 3.0542368279738827E-4 1.2862252741522204 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7165 0.6948451508730417 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6015 0.5833208070483386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3614 0.35047737268041484 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2983 0.2892844501122517 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1729 0.1676744264981841 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1601 0.1552612821420432 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1176 0.11404576377204423 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1125 0.10909990156764438 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 9.697769028235054E-5 16-17 0.0 0.0 0.0 0.0 9.697769028235054E-5 18-19 0.0 0.0 0.0 1.9395538056470109E-4 9.697769028235054E-5 20-21 0.0 0.0 0.0 1.9395538056470109E-4 9.697769028235054E-5 22-23 0.0 0.0 0.0 3.8791076112940217E-4 9.697769028235054E-5 24-25 0.0 0.0 0.0 6.303549868352785E-4 9.697769028235054E-5 26-27 0.0 0.0 0.0 0.0014061765090940827 9.697769028235054E-5 28-29 0.0 0.0 0.0 0.005382261810670455 9.697769028235054E-5 30-31 0.0 0.0 0.0 0.013673854329811427 9.697769028235054E-5 32-33 0.0 0.0 0.0 0.030402505903516897 9.697769028235054E-5 34-35 0.0 0.0 0.0 0.05794416994370445 9.697769028235054E-5 36-37 0.0 0.0 0.0 0.10202053017703278 9.697769028235054E-5 38-39 0.0 0.0 0.0 0.16020714434644312 1.4546653542352582E-4 40-41 0.0 0.0 0.0 0.2230971764945474 1.9395538056470109E-4 42-43 0.0 0.0 0.0 0.2854538313460988 1.9395538056470109E-4 44-45 0.0 0.0 0.0 0.3673514907895439 1.9395538056470109E-4 46-47 0.0 0.0 0.0 0.4641352256913297 1.9395538056470109E-4 48-49 0.0 0.0 0.0 0.5651859789655389 1.9395538056470109E-4 50-51 0.0 0.0 0.0 0.6751101909005833 1.9395538056470109E-4 52-53 0.0 0.0 0.0 0.7725242807892044 1.9395538056470109E-4 54-55 0.0 0.0 0.0 0.8821575596534017 1.9395538056470109E-4 56-57 0.0 0.0 0.0 1.0162292164687514 1.9395538056470109E-4 58-59 0.0 0.0 0.0 1.149525051761842 1.9395538056470109E-4 60-61 0.0 0.0 0.0 1.2903851468969565 1.9395538056470109E-4 62-63 0.0 0.0 0.0 1.4374033253649998 1.9395538056470109E-4 64-65 0.0 0.0 0.0 1.5812212400537256 1.9395538056470109E-4 66-67 0.0 0.0 0.0 1.748653222326204 1.9395538056470109E-4 68-69 0.0 0.0 0.0 1.930146969689623 1.9395538056470109E-4 70-71 0.0 0.0 0.0 2.1297270562907 1.9395538056470109E-4 72-73 0.0 0.0 0.0 2.3144695562785778 1.9395538056470109E-4 74-75 0.0 0.0 0.0 2.497030058235103 1.9395538056470109E-4 76-77 0.0 0.0 0.0 2.701168096279451 1.9395538056470109E-4 78-79 0.0 0.0 0.0 2.9297930011200926 1.9395538056470109E-4 80-81 0.0 0.0 0.0 3.1722857156711095 1.9395538056470109E-4 82-83 0.0 0.0 0.0 3.413566209093598 1.9395538056470109E-4 84-85 0.0 0.0 0.0 3.6518888829624743 1.9395538056470109E-4 86-87 0.0 0.0 0.0 3.896854528615692 1.9395538056470109E-4 88-89 0.0 0.0 0.0 4.155348562063297 1.9395538056470109E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACG 60 0.0039400207 31.684286 4 GGTATCA 6410 0.0 27.324986 1 GTATCAA 8730 0.0 25.967566 1 GTACATG 5305 0.0 24.65001 1 GAGTACT 4750 0.0 24.59936 12-13 GGACCGA 235 1.7462298E-10 24.266459 6 TACATGG 5315 0.0 23.874989 2 GTAAGCG 140 1.8189894E-12 23.770716 94-95 AGGACCG 305 0.0 23.373653 5 ACATGGG 5350 0.0 22.475115 3 GTACTTT 5310 0.0 22.320864 14-15 TATCAAC 10105 0.0 22.199383 2 TCAACGC 10105 0.0 22.15235 4 ATCAACG 10140 0.0 22.029016 3 CAACGCA 10185 0.0 21.978348 5 AACGCAG 10375 0.0 21.71117 6 AGTACTT 4915 0.0 20.777689 12-13 AGAGTAC 7895 0.0 20.546696 10-11 TACCTGG 765 0.0 20.501596 2 GTACCTG 745 0.0 20.499607 1 >>END_MODULE