FastQCFastQC Report
Thu 26 May 2016
SRR938267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031165
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70120.680007564259842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60990.591466933032056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38380.37220037530366135No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29720.28821769551914583No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16910.16398927426745477No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16390.15894643437277253No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12110.11743998293192652No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11050.10716034776199734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA1850.038.5093276
ATACCGT650.005846902329.227596
GGTATCA63450.026.2357771
GTATCAA84850.024.4955441
ACCGAGT2950.024.1499188
GTACATG52200.023.7808571
AGGACCG3200.023.7797055
TACATGG51300.023.6417882
ACATGGG51750.022.9766773
GAGTACT50150.022.58727512-13
TACCTGG6550.022.5101282
GTACTTT53950.021.04034614-15
ATCAACG99550.020.7350923
TATCAAC100100.020.7161922
TCAACGC100450.020.5483154
CAACGCA101200.020.490025
AGTACTT51650.020.41404312-13
TCGTATC704.8570964E-420.35492994-95
AACGCAG102650.020.1731596
TAGACTG3800.020.0250155
Produced by FastQC (version 0.11.5)