FastQCFastQC Report
Thu 26 May 2016
SRR938266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006274
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70180.6974243595680699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58330.5796631931263254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35570.35348225234876385No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28930.2874962485366809No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16860.1675487988361023No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15880.15780990068311415No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11950.11875493155939634No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.10851915084758228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83600.026.4191131
GTACATG49700.026.394891
TACCTGG6850.025.6716062
GGTATCA61650.025.5343281
TACATGG49000.025.5094782
ACATGGG50050.024.2158433
GAGTACT49100.024.04260812-13
GTACCTG8100.022.9679991
TATCAAC96800.022.5361232
GTACTTT52050.022.40727614-15
ATCAACG97650.022.3410683
TCAACGC98100.022.1901364
CAACGCA98200.022.1191375
CGATCGT1406.548362E-1122.05521224-25
AACGCAG99950.021.9188026
AATTCGC1306.2937033E-1021.914978-79
ATTCGCG1351.0059011E-921.10428680-81
CAATTCG1351.0077201E-921.10323578-79
AGTACTT50200.021.05537412-13
ACGCGTA1150.003712788220.6619266
Produced by FastQC (version 0.11.5)