##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938266_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1006274 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.860092777911383 33.0 31.0 34.0 30.0 34.0 2 32.032463325098334 33.0 31.0 34.0 30.0 34.0 3 32.10465638583527 34.0 31.0 34.0 30.0 34.0 4 35.6331506130537 37.0 35.0 37.0 33.0 37.0 5 35.65139514684867 37.0 35.0 37.0 35.0 37.0 6 35.681680138809114 37.0 35.0 37.0 35.0 37.0 7 35.628345758709855 37.0 35.0 37.0 33.0 37.0 8 35.609786201372586 37.0 35.0 37.0 33.0 37.0 9 37.230585307778995 39.0 37.0 39.0 34.0 39.0 10-11 37.14027243076936 39.0 37.0 39.0 34.0 39.0 12-13 37.02998884995538 39.0 37.0 39.0 33.0 39.0 14-15 38.18053532139358 40.0 38.0 41.0 33.0 41.0 16-17 38.03816505246086 40.0 38.0 41.0 33.0 41.0 18-19 38.20666885957503 40.0 38.0 41.0 33.5 41.0 20-21 38.20663854973894 40.0 38.0 41.0 34.0 41.0 22-23 38.110333269069855 40.0 38.0 41.0 33.0 41.0 24-25 37.98447689197972 40.0 38.0 41.0 33.0 41.0 26-27 37.79910690328877 40.0 38.0 41.0 33.0 41.0 28-29 37.58659569858706 40.0 37.5 41.0 32.0 41.0 30-31 37.36301345359216 40.0 37.0 41.0 32.0 41.0 32-33 37.089981456342905 40.0 36.0 41.0 31.0 41.0 34-35 36.83806100525304 39.0 36.0 41.0 30.5 41.0 36-37 36.56481187032558 39.0 35.0 41.0 30.0 41.0 38-39 36.27652905669827 39.0 35.0 40.5 30.0 41.0 40-41 36.0486949876475 39.0 35.0 40.0 30.0 41.0 42-43 35.83981549756825 39.0 35.0 40.0 28.5 41.0 44-45 35.52918688150544 39.0 35.0 40.0 27.0 41.0 46-47 35.083757008528494 38.0 35.0 40.0 25.0 41.0 48-49 34.6533727394328 38.0 34.0 40.0 23.5 41.0 50-51 33.941788717585865 37.5 33.5 39.5 23.0 40.5 52-53 34.08316323387069 38.0 33.5 40.0 23.0 40.5 54-55 34.18541818629916 38.0 34.0 40.0 22.5 41.0 56-57 33.881054762420575 38.0 33.5 40.0 20.5 41.0 58-59 33.56660412571526 38.0 33.0 40.0 19.5 41.0 60-61 33.131151157636985 37.0 33.0 40.0 17.5 41.0 62-63 32.59498754812307 36.5 32.0 39.5 12.0 41.0 64-65 32.07792062599252 36.0 31.0 39.0 8.0 40.5 66-67 31.519604998241036 35.0 31.0 39.0 4.5 40.0 68-69 30.90870776746691 35.0 30.0 38.0 2.0 40.0 70-71 30.339820466393846 35.0 29.5 37.5 2.0 40.0 72-73 29.688726430375823 34.5 29.0 37.0 2.0 39.0 74-75 28.99363990324703 34.0 28.0 36.0 2.0 39.0 76-77 28.313268553097863 34.0 26.5 36.0 2.0 38.5 78-79 27.581974193907428 33.0 26.0 35.0 2.0 37.0 80-81 26.888486137970375 33.0 24.5 35.0 2.0 37.0 82-83 26.151382227902143 32.0 23.0 35.0 2.0 36.0 84-85 25.477663141450538 32.0 20.0 35.0 2.0 36.0 86-87 24.7770090452501 31.0 17.5 34.0 2.0 35.0 88-89 23.953029691714185 31.0 8.5 34.0 2.0 35.0 90-91 23.10593983348472 30.5 2.0 34.0 2.0 35.0 92-93 22.18451336315954 29.5 2.0 34.0 2.0 35.0 94-95 21.22811629834419 29.0 2.0 34.0 2.0 35.0 96-97 20.01388140804592 28.0 2.0 34.0 2.0 35.0 98-99 18.541453918117732 25.5 2.0 33.0 2.0 35.0 100-101 14.961807618998405 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2551519118365668 1101 2 0.2529681552092313 1101 3 0.10537139145733931 1101 4 0.16601786168823196 1101 5 0.2140398505206349 1101 6 0.309822887670947 1101 7 0.24732828898901005 1101 8 0.22238478292020858 1101 9 0.1930647418038447 1101 10-11 0.31827022720130316 1101 12-13 0.3751636802473399 1101 14-15 0.43551036229730755 1101 16-17 0.6221354421036764 1101 18-19 0.4875765646036925 1101 20-21 0.4697622128863941 1101 22-23 0.44563583403939333 1101 24-25 0.3331244322466844 1101 26-27 0.534914239338768 1101 28-29 0.6290701961830294 1101 30-31 0.6663243768746341 1101 32-33 0.5342062905015865 1101 34-35 0.707834003976771 1101 36-37 0.7332346310842155 1101 38-39 0.8510690245045467 1101 40-41 1.0483169379658577 1101 42-43 1.1336492769106101 1101 44-45 1.1859229957658712 1101 46-47 1.4431039314443694 1101 48-49 1.318849315744572 1101 50-51 1.0438058620970239 1101 52-53 1.1717279551337683 1101 54-55 1.3562349508877745 1101 56-57 1.289702728795369 1101 58-59 1.4647296705607786 1101 60-61 1.4234949781435162 1101 62-63 1.7250860773744705 1101 64-65 1.7098397315995015 1101 66-67 1.8304149891790438 1101 68-69 1.9252817690942656 1101 70-71 2.153633327059204 1101 72-73 2.3713212635788814 1101 74-75 2.215952782695517 1101 76-77 2.698714907282607 1101 78-79 2.482811474282567 1101 80-81 2.653141678450641 1101 82-83 2.7828800025957925 1101 84-85 2.9137315073723506 1101 86-87 3.3238345306568498 1101 88-89 3.7632787758292494 1101 90-91 3.8710409557908676 1101 92-93 3.8990307148096264 1101 94-95 4.229774981714957 1101 96-97 4.557489503737749 1101 98-99 4.202694723746909 1101 100-101 4.2748653824379605 1102 1 0.2313952557857455 1102 2 0.2742484854423992 1102 3 0.07635650812591166 1102 4 0.07782069455421947 1102 5 0.11242590358294535 1102 6 0.1515325886931933 1102 7 0.19708277111759287 1102 8 0.16087737508986066 1102 9 0.22034979207050753 1102 10-11 0.3285773254380615 1102 12-13 0.3146010192650124 1102 14-15 0.35674956141420466 1102 16-17 0.25760008626136965 1102 18-19 0.16464866353830132 1102 20-21 0.29741807761260475 1102 22-23 0.2924155531212662 1102 24-25 0.3117129899719373 1102 26-27 0.34750437886734886 1102 28-29 0.34104506073582996 1102 30-31 0.25568463470012404 1102 32-33 0.29817712441646904 1102 34-35 0.24219751845619442 1102 36-37 0.5468863552075618 1102 38-39 0.6027280147726586 1102 40-41 0.4171488565579651 1102 42-43 0.7133183458882257 1102 44-45 0.7730786680534365 1102 46-47 0.8089953452974115 1102 48-49 0.8009303176097475 1102 50-51 0.922064102065896 1102 52-53 0.855964164050242 1102 54-55 0.8834322406749848 1102 56-57 0.8404134829433758 1102 58-59 0.9862859704943716 1102 60-61 1.154682514886602 1102 62-63 1.1302148843817719 1102 64-65 1.1941385359422725 1102 66-67 1.075702885845839 1102 68-69 1.3422085451337473 1102 70-71 1.3412070775825953 1102 72-73 1.5721997142574935 1102 74-75 1.696230993988916 1102 76-77 1.7033561678191482 1102 78-79 1.9447472166939264 1102 80-81 1.9784169080024476 1102 82-83 2.240292908250108 1102 84-85 2.416948510489437 1102 86-87 2.6947816882746736 1102 88-89 2.9398428156027236 1102 90-91 3.1521721355031858 1102 92-93 3.470425979550214 1102 94-95 3.608080273989078 1102 96-97 3.7029170770230344 1102 98-99 4.301680614399466 1102 100-101 3.977676149697169 1103 1 0.3407129759628056 1103 2 0.3603042752312646 1103 3 0.4539424034141817 1103 4 0.23337634002658803 1103 5 0.3948505825124684 1103 6 0.30254833402718617 1103 7 0.367625751806159 1103 8 0.3848633156962009 1103 9 0.3752769189853993 1103 10-11 0.4284416409597682 1103 12-13 0.6228913948385753 1103 14-15 0.6477077959514332 1103 16-17 0.6956384070108399 1103 18-19 0.6442143922826418 1103 20-21 0.6089710849255354 1103 22-23 0.6260820282651096 1103 24-25 0.7366677393333063 1103 26-27 0.7461436227028813 1103 28-29 0.6653903089467121 1103 30-31 0.9091308316233437 1103 32-33 1.0064366135630536 1103 34-35 0.9466831987533482 1103 36-37 1.014787399951068 1103 38-39 0.8825893900144237 1103 40-41 1.028729108666738 1103 42-43 1.1988974866251354 1103 44-45 1.2469223152953717 1103 46-47 1.332090030404224 1103 48-49 1.171065705362544 1103 50-51 1.4289683649323166 1103 52-53 1.4545566061820168 1103 54-55 1.5764157616197636 1103 56-57 1.7362533914440235 1103 58-59 1.7894032067391237 1103 60-61 1.7757923761539445 1103 62-63 1.5833942981061426 1103 64-65 1.5775894804734776 1103 66-67 1.784252356730324 1103 68-69 2.028105721655752 1103 70-71 2.375491011515315 1103 72-73 2.4710668972265246 1103 74-75 2.3298019774720977 1103 76-77 2.5808031416236865 1103 78-79 2.8052127250521437 1103 80-81 2.7292733656934374 1103 82-83 2.976870800042409 1103 84-85 3.0963689786143718 1103 86-87 3.1635553757364008 1103 88-89 3.3547088979899016 1103 90-91 3.1971118867393145 1103 92-93 3.115310971444213 1103 94-95 3.326073708263028 1103 96-97 3.3360773654402216 1103 98-99 3.811898087215365 1103 100-101 3.7652537366714594 1104 1 0.4460676089369464 1104 2 0.3224530805269552 1104 3 0.3383812727397739 1104 4 0.35305331898387493 1104 5 0.2505792690178268 1104 6 0.2876038249315158 1104 7 0.33828584923639937 1104 8 0.3478458699402651 1104 9 0.3450654842823013 1104 10-11 0.47663535283024316 1104 12-13 0.45969070567328174 1104 14-15 0.5786023506388176 1104 16-17 0.7056257622382773 1104 18-19 0.5954274844081198 1104 20-21 0.6104924878403821 1104 22-23 0.6099032684603785 1104 24-25 0.5055492563029915 1104 26-27 0.6683253749358684 1104 28-29 0.6622832717025702 1104 30-31 0.5769070644886725 1104 32-33 0.6570983230758571 1104 34-35 0.6871996193255328 1104 36-37 0.7239437769970252 1104 38-39 0.7330208739867956 1104 40-41 0.7336739200278899 1104 42-43 0.5902330466474979 1104 44-45 0.7143998091115193 1104 46-47 0.729441932913538 1104 48-49 1.0348157620373186 1104 50-51 0.7527009909644278 1104 52-53 0.7339431080132428 1104 54-55 0.814399449359378 1104 56-57 1.1082338852450562 1104 58-59 0.9697196786964426 1104 60-61 1.2082196687116706 1104 62-63 1.2493884240803155 1104 64-65 1.3783537083615016 1104 66-67 1.3794156052781936 1104 68-69 1.4515607672222721 1104 70-71 1.577959764821344 1104 72-73 1.6557082844235715 1104 74-75 1.936921085184398 1104 76-77 1.8782890484956916 1104 78-79 2.0864040036855016 1104 80-81 2.1723315870625974 1104 82-83 2.4622398913820014 1104 84-85 2.3241058665897505 1104 86-87 2.403211599130742 1104 88-89 2.613832277720988 1104 90-91 2.677993595143807 1104 92-93 3.2121127507803386 1104 94-95 3.3779260918782263 1104 96-97 3.595466486905604 1104 98-99 3.949267672255239 1104 100-101 3.914924267298508 1105 1 0.33040176486419526 1105 2 0.2799246168062126 1105 3 0.17454350474709912 1105 4 -0.0261096065830273 1105 5 0.16066294420254934 1105 6 0.19812388708305662 1105 7 0.3743124159731437 1105 8 0.4102241161843807 1105 9 0.28203489776746693 1105 10-11 0.34287513089117994 1105 12-13 0.239477924463543 1105 14-15 0.4123622141491623 1105 16-17 0.5020633391884388 1105 18-19 0.42111911742728836 1105 20-21 0.3690212992369837 1105 22-23 0.4804164129376147 1105 24-25 0.511462434114101 1105 26-27 0.6211610837555455 1105 28-29 0.7069439382724596 1105 30-31 0.6941540272533047 1105 32-33 0.6812824048979564 1105 34-35 0.9605270565064217 1105 36-37 0.8061847988056243 1105 38-39 0.9267511492062894 1105 40-41 0.9292433826177913 1105 42-43 0.9247626358344192 1105 44-45 0.7311559037438755 1105 46-47 0.6235168373474735 1105 48-49 0.712328929812422 1105 50-51 0.7473366571310862 1105 52-53 0.733279118809115 1105 54-55 0.9629887635239385 1105 56-57 0.9907436389866007 1105 58-59 0.6336873771001805 1105 60-61 1.0799859696018288 1105 62-63 1.1689166960416912 1105 64-65 1.2827164131363133 1105 66-67 1.4834002573603975 1105 68-69 1.4699181421620118 1105 70-71 1.569521904712488 1105 72-73 1.5286681529519157 1105 74-75 1.5149810359721378 1105 76-77 1.755277655838821 1105 78-79 1.8575866930120277 1105 80-81 2.0847822359081896 1105 82-83 2.277009753261492 1105 84-85 2.5074609744678327 1105 86-87 2.5850173988658653 1105 88-89 2.878016646286074 1105 90-91 2.945292261251325 1105 92-93 3.389032270987652 1105 94-95 3.6172762785299817 1105 96-97 3.915661167464968 1105 98-99 4.023902836621687 1105 100-101 4.019172364490041 1106 1 0.07395007957179445 1106 2 0.2499641563417896 1106 3 0.053908760117231225 1106 4 0.06647961257935009 1106 5 0.1278353613943679 1106 6 0.022862453574688857 1106 7 0.0738022653216106 1106 8 0.14287771799582316 1106 9 0.09957133741401236 1106 10-11 0.23491847037588798 1106 12-13 0.2511818205994416 1106 14-15 0.4020993593999762 1106 16-17 0.13988453741325202 1106 18-19 0.23348848867563277 1106 20-21 0.16864526443726646 1106 22-23 0.004621068095310932 1106 24-25 0.06435283312635676 1106 26-27 0.18087871884602436 1106 28-29 0.17231934385410597 1106 30-31 0.15579680563389786 1106 32-33 0.1700048810074719 1106 34-35 0.19301521356629792 1106 36-37 0.05726700988672917 1106 38-39 -0.07361186121937635 1106 40-41 0.4886917299743274 1106 42-43 0.5668277559446722 1106 44-45 0.6019642333591051 1106 46-47 0.9686447094199551 1106 48-49 0.8897904265066074 1106 50-51 0.9165945582180015 1106 52-53 1.1102793062545047 1106 54-55 0.5458708638978607 1106 56-57 0.8192966750298041 1106 58-59 1.2293016831430563 1106 60-61 1.139467757918787 1106 62-63 0.9517018176303793 1106 64-65 1.1691456313834578 1106 66-67 1.5209861368268456 1106 68-69 1.201066900754359 1106 70-71 1.3221762809461737 1106 72-73 1.725205517505028 1106 74-75 1.6793385163189711 1106 76-77 1.8221072151837632 1106 78-79 1.8803133457214969 1106 80-81 1.9465997873082692 1106 82-83 2.3569472890675236 1106 84-85 2.426062156693785 1106 86-87 2.4641842085366363 1106 88-89 2.6232374490275276 1106 90-91 2.635995498248292 1106 92-93 3.094710018052549 1106 94-95 3.1620119233376975 1106 96-97 3.3148802330365292 1106 98-99 3.530103934717225 1106 100-101 3.97025087946499 1107 1 0.386919098902343 1107 2 0.23255000862189945 1107 3 0.07740517362063315 1107 4 0.1596099479671338 1107 5 0.2715140684943904 1107 6 0.32203913310745946 1107 7 0.3528889838189002 1107 8 0.2489424589022846 1107 9 0.4241198538590609 1107 10-11 0.21432935550424048 1107 12-13 0.31587215776586675 1107 14-15 0.34056655169669625 1107 16-17 0.3510658449085824 1107 18-19 0.36892309254802313 1107 20-21 0.45606937779619017 1107 22-23 0.4802488466820094 1107 24-25 0.44659960955165445 1107 26-27 0.5602356343281585 1107 28-29 0.6123432858005557 1107 30-31 0.5967414575384069 1107 32-33 0.5498905943963948 1107 34-35 0.5727657848685652 1107 36-37 0.6513298256723701 1107 38-39 0.6065709031948359 1107 40-41 0.757111378413093 1107 42-43 0.8338920600370372 1107 44-45 0.7085586440567724 1107 46-47 0.8504542358036176 1107 48-49 0.9803338807906599 1107 50-51 0.6986940733630789 1107 52-53 0.7161679265061096 1107 54-55 0.7812700692623977 1107 56-57 0.9379270143410636 1107 58-59 1.1161602922606946 1107 60-61 1.2121689692278252 1107 62-63 1.2428898560735107 1107 64-65 1.3978862553562692 1107 66-67 1.4159824792716726 1107 68-69 1.6042855493160495 1107 70-71 1.7794513282241056 1107 72-73 2.0198558090195853 1107 74-75 2.2449687449009126 1107 76-77 2.3779361549461413 1107 78-79 2.6849567406302164 1107 80-81 2.717632909325875 1107 82-83 2.6402718018367075 1107 84-85 2.8459106929306124 1107 86-87 2.933032213795844 1107 88-89 3.203475229349273 1107 90-91 3.2488666049573567 1107 92-93 3.7301755738221978 1107 94-95 4.184376630850988 1107 96-97 4.436409152135248 1107 98-99 4.9317850150866605 1107 100-101 4.510759501966062 1108 1 0.22736743485312516 1108 2 0.16431086743049406 1108 3 0.18179688672004346 1108 4 0.1706393565681239 1108 5 0.180878782475844 1108 6 0.16405965673975942 1108 7 0.18913707451872597 1108 8 0.18963045236225895 1108 9 0.21769772103630913 1108 10-11 0.1861465790053103 1108 12-13 0.2314154746774335 1108 14-15 0.43427435885200083 1108 16-17 0.39528071916368646 1108 18-19 0.21593813030376197 1108 20-21 0.15659301009186777 1108 22-23 0.13346529140373065 1108 24-25 0.21281435472730692 1108 26-27 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-1.1737403688647454 2316 66-67 -1.2335540807212233 2316 68-69 -1.3244491302511072 2316 70-71 -1.4781216485569821 2316 72-73 -1.4368465820049359 2316 74-75 -1.736247984699002 2316 76-77 -1.5816019358555096 2316 78-79 -2.322839273341298 2316 80-81 -2.4768357683516378 2316 82-83 -2.2918183117723494 2316 84-85 -2.461017698140001 2316 86-87 -2.1476060182352583 2316 88-89 -2.387419421379427 2316 90-91 -2.4215062375076073 2316 92-93 -2.6351560520283854 2316 94-95 -3.060848233433763 2316 96-97 -3.3009122946600975 2316 98-99 -3.708677799428699 2316 100-101 -3.1260231989073244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2805.0 3 898.0 4 915.0 5 1088.0 6 1210.0 7 1486.0 8 1845.0 9 2406.0 10 2955.0 11 3645.0 12 4642.0 13 5824.0 14 6835.0 15 8530.0 16 10492.0 17 13322.0 18 12917.0 19 9922.0 20 9880.0 21 10668.0 22 11734.0 23 13045.0 24 14557.0 25 16375.0 26 18735.0 27 21271.0 28 24492.0 29 28239.0 30 33663.0 31 40498.0 32 48755.0 33 60670.0 34 79969.0 35 108029.0 36 137066.0 37 152961.0 38 79616.0 39 4313.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7912434147408 18.569707158752045 14.491713330451478 22.147336096055685 2 15.479717343152888 20.239212473804137 40.468483443418926 23.812586739624052 3 19.882138528491474 24.840393852590637 29.500465818284137 25.77700180063375 4 12.794252902135275 16.86514703323473 36.81195157722041 33.528648487409576 5 13.58366900392859 37.539808046148494 33.54259286886469 15.333930081058234 6 31.45658182512584 35.93092795911852 17.4020804759693 15.210409739786343 7 28.42067377480125 33.22512716296346 20.255896822019576 18.09830224021571 8 26.489796283574535 34.19882906053433 19.32050118356549 19.990873472325642 9 26.583364568306163 17.206807997876354 20.344754562708722 35.86507287110876 10-11 25.273479192216662 26.657367172753343 26.375648184833906 21.693505450196092 12-13 26.563487222620736 24.31074928379368 27.172591928679125 21.953171564906462 14-15 23.126661483798234 25.42634395026037 26.479503478553063 24.967491087388332 16-17 21.79222839805029 28.88343988873568 26.896896588404534 22.4274351248095 18-19 21.559576018667745 27.105813388162048 29.860787294479312 21.473823298690892 20-21 22.622373293918137 25.771810478933403 30.089782923098106 21.51603330405035 22-23 22.157740499454412 25.924254136642816 30.099498159420758 21.818507204482014 24-25 22.211360948040074 26.087808263926977 30.143300867183797 21.557529920849156 26-27 21.81237216526316 26.192635327082918 30.32252387159899 21.67246863605494 28-29 21.449907911187115 26.808805728644963 30.224617166547773 21.516669193620146 30-31 21.631984489958242 26.165490157088882 30.18547424935375 22.017051103599126 32-33 21.583913996411876 26.307678771070524 30.12382452698971 21.984582705527885 34-35 22.220139784074917 26.303099479948354 29.874757088009083 21.602003647967642 36-37 22.09948623880575 25.99542163464048 30.028707991260838 21.876384135292934 38-39 21.671124037846486 26.463166673542005 30.044537264236045 21.821172024375468 40-41 21.940778748817124 26.4150755846779 29.85490405795488 21.789241608550096 42-43 21.741550612826895 26.40143056724198 29.536760419250037 22.32025840068109 44-45 21.840030970660866 26.40865071386081 29.530763676265153 22.22055463921317 46-47 22.243295927506455 26.88080334555012 29.00268689742374 21.87321382951969 48-49 22.13272537772324 26.72449998484087 28.89394524522748 22.248829392208407 50-51 21.886483021155843 27.11799464270628 28.982561462272827 22.012960873865055 52-53 22.30161447666794 27.175607571894083 28.42397319194466 22.09880475949332 54-55 21.838309875673826 26.96511839984654 28.740383071920533 22.456188652559106 56-57 22.093714374314818 27.114531949934452 28.586969161760862 22.204784513989868 58-59 22.479224831010736 26.670983541871866 28.55652704573668 22.29326458138072 60-61 22.011583422208485 26.849605354574578 29.085283368436123 22.05352785478082 62-63 21.858255351081645 27.035746765993608 29.007111583781015 22.09888629914373 64-65 21.646357220952194 27.25862240333963 28.699731637014214 22.395288738693967 66-67 21.769529615564657 27.12862647426844 28.450312420180545 22.651531489986354 68-69 21.796674823235687 26.95211013952305 28.625233813914935 22.62598122332633 70-71 21.99686405868335 27.043393774336472 28.438415529618744 22.521326637361437 72-73 22.064209742681957 26.833971269514535 28.630993262161457 22.47082572564205 74-75 21.77644064840173 27.51791383963691 28.211061035019636 22.49458447694172 76-77 22.02334572342921 27.030402114929736 28.182830109920694 22.76342205172037 78-79 22.16566340052309 27.227432226734006 28.114195921900787 22.492708450842112 80-81 22.23078111977879 27.26169005979025 28.082731297718272 22.42479752271269 82-83 22.210648619798214 27.196679127063245 28.202075098323164 22.390597154815374 84-85 22.179854602811435 27.190635900254488 28.09572818110393 22.533781315830144 86-87 22.314486122484674 27.128286518417223 27.95816471748066 22.59906264161744 88-89 22.217853887315513 27.077805953814053 27.773696615330174 22.93064354354026 90-91 22.341926707056366 27.019398091057255 27.858654932479215 22.78002026940716 92-93 22.658352027687194 26.813368889878415 27.860434928242505 22.66784415419189 94-95 22.345031684311035 26.70374311775211 27.902125326622524 23.04909987131433 96-97 22.334339138374155 26.63595738938671 28.027069757408967 23.002633714830164 98-99 22.45798449843568 26.740770008772124 27.441247878412867 23.35999761437933 100-101 23.281332417719124 25.665611719861865 27.553717692254104 23.49933817016491 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1333.0 1 893.0 2 740.5 3 972.0 4 663.0 5 524.5 6 960.0 7 1909.5 8 3100.0 9 3593.5 10 2842.0 11 1903.5 12 1706.0 13 1746.5 14 1772.0 15 1866.5 16 1960.0 17 2035.5 18 2125.5 19 2215.0 20 2361.5 21 2626.5 22 3006.5 23 3582.0 24 4238.0 25 4967.5 26 5937.0 27 7082.5 28 8144.0 29 9499.0 30 11253.5 31 12836.5 32 14541.5 33 15963.5 34 17156.5 35 18499.5 36 20187.5 37 22225.5 38 23829.0 39 24978.5 40 26274.5 41 28506.5 42 30509.0 43 32072.0 44 34148.5 45 35863.5 46 36369.5 47 38317.5 48 42714.0 49 43316.5 50 40143.5 51 37049.5 52 35052.5 53 34304.0 54 33201.5 55 30964.5 56 27984.5 57 25419.5 58 23280.0 59 20966.0 60 18072.5 61 14729.5 62 11497.5 63 8770.5 64 6755.5 65 5333.5 66 4191.0 67 3355.5 68 2596.5 69 1875.5 70 1373.0 71 1026.5 72 825.0 73 667.5 74 513.5 75 400.0 76 273.5 77 172.0 78 105.0 79 57.0 80 29.0 81 24.5 82 17.0 83 12.0 84 11.0 85 6.5 86 4.0 87 2.5 88 1.0 89 0.5 90 1.0 91 0.5 92 1.0 93 1.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45762883667867804 2 0.03875683958842224 3 0.05137765658260077 4 0.05455770495908669 5 0.08188624569451264 6 0.08258188127686893 7 0.04750197262375854 8 0.04124125238255187 9 0.04541506587668965 10-11 0.048147919950232244 12-13 0.0450672480855115 14-15 0.03935309865901335 16-17 0.03786245098253557 18-19 0.04720384308846298 20-21 0.056694299962038175 22-23 0.047352907856110765 24-25 0.06583693904443522 26-27 0.057737753335572614 28-29 0.04486849506198113 30-31 0.0471044665766978 32-33 0.04526600110904187 34-35 0.050532956232596696 36-37 0.04536537762080706 38-39 0.026086334338361123 40-41 0.024844127941296305 42-43 0.02380067456776186 44-45 0.01594993013831223 46-47 0.02792479980601705 48-49 0.027775735038369272 50-51 0.016794630488316302 52-53 0.01575117711478186 54-55 0.01694369525596408 56-57 0.01520460630007334 58-59 0.01440959420595186 60-61 0.017987148629498524 62-63 0.016794630488316302 64-65 0.01729151304714223 66-67 0.009639521641222967 68-69 0.01311769955300445 70-71 0.019527484561858897 72-73 0.016397124441255562 74-75 0.013763646879478154 76-77 0.009341392105927411 78-79 0.014260529438304081 80-81 0.017241824791259637 82-83 0.01520460630007334 84-85 0.013266764320652227 86-87 0.0041738134941377795 88-89 0.029514823994260014 90-91 0.032247678067802606 92-93 0.017589642582437785 94-95 0.03324144318545446 96-97 0.02787511155013446 98-99 0.02449631015011816 100-101 0.14747474345953487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1006274.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.99940167653031 #Duplication Level Percentage of deduplicated Percentage of total 1 84.43178768313692 54.035838942006045 2 9.178891669201242 11.748871497651363 3 2.5866942977363925 4.966406621256656 4 1.1189452799300832 2.8644731369721224 5 0.6617360070343292 2.117535425900666 6 0.4110263365435386 1.5783263767249596 7 0.27200845842489413 1.2185865013104016 8 0.20202193114339917 1.03434261749718 9 0.15210720454057947 0.876129307315803 >10 0.8925341386423224 10.410893563575128 >50 0.061402134270688885 2.6493776754017104 >100 0.02772921754840044 3.1866988908277807 >500 0.001869385108321363 0.8337878135889841 >1k 9.346925541606815E-4 1.1974919800331565 >5k 3.1156418472022714E-4 1.2812396499380647 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7018 0.6974243595680699 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5833 0.5796631931263254 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3557 0.35348225234876385 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2893 0.2874962485366809 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1686 0.1675487988361023 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1588 0.15780990068311415 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1195 0.11875493155939634 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1092 0.10851915084758228 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.937651176518523E-5 0.0 14-15 0.0 0.0 0.0 9.937651176518523E-5 0.0 16-17 0.0 0.0 0.0 9.937651176518523E-5 0.0 18-19 0.0 0.0 0.0 9.937651176518523E-5 0.0 20-21 0.0 0.0 0.0 1.4906476764777786E-4 0.0 22-23 0.0 0.0 0.0 4.4719430294333355E-4 0.0 24-25 0.0 0.0 0.0 6.956355823562967E-4 9.937651176518523E-5 26-27 0.0 0.0 0.0 0.0020869067470688897 9.937651176518523E-5 28-29 0.0 0.0 0.0 0.005714149426498151 9.937651176518523E-5 30-31 0.0 0.0 0.0 0.01619837141772519 9.937651176518523E-5 32-33 0.0 0.0 0.0 0.036471179817822975 9.937651176518523E-5 34-35 0.0 0.0 0.0 0.061812190317945215 9.937651176518523E-5 36-37 0.0 0.0 0.0 0.10454409037697486 9.937651176518523E-5 38-39 0.0 0.0 0.0 0.16744942232433713 9.937651176518523E-5 40-41 0.0 0.0 0.0 0.23447887950995455 9.937651176518523E-5 42-43 0.0 0.0 0.0 0.30245241355734126 1.4906476764777786E-4 44-45 0.0 0.0 0.0 0.38289769983125865 1.9875302353037047E-4 46-47 0.0 0.0 0.0 0.4781003981023061 2.4844127941296305E-4 48-49 0.0 0.0 0.0 0.5784706749851432 2.9812953529555566E-4 50-51 0.0 0.0 0.0 0.6839588422238874 2.9812953529555566E-4 52-53 0.0 0.0 0.0 0.7867638436449714 3.478177911781483E-4 54-55 0.0 0.0 0.0 0.8967239539131489 3.9750604706074094E-4 56-57 0.0 0.0 0.0 1.0246215245549424 3.9750604706074094E-4 58-59 0.0 0.0 0.0 1.1642952118409102 3.9750604706074094E-4 60-61 0.0 0.0 0.0 1.3089377247151373 3.9750604706074094E-4 62-63 0.0 0.0 0.0 1.4516920838658258 3.9750604706074094E-4 64-65 0.0 0.0 0.0 1.5900241882429635 3.9750604706074094E-4 66-67 0.0 0.0 0.0 1.7550885742849363 3.9750604706074094E-4 68-69 0.0 0.0 0.0 1.9420157929152497 3.9750604706074094E-4 70-71 0.0 0.0 0.0 2.1317255538749884 3.9750604706074094E-4 72-73 0.0 0.0 0.0 2.325112245770039 4.968825588259261E-4 74-75 0.0 0.0 0.0 2.5167598487091984 4.968825588259261E-4 76-77 0.0 0.0 0.0 2.71745071421899 4.968825588259261E-4 78-79 0.0 0.0 0.0 2.951482399426001 4.968825588259261E-4 80-81 0.0 0.0 0.0 3.202805597680155 4.968825588259261E-4 82-83 0.0 0.0 0.0 3.4535822251195993 4.968825588259261E-4 84-85 0.0 0.0 0.0 3.697998755806073 4.968825588259261E-4 86-87 0.0 0.0 0.0 3.953147949763186 5.962590705911113E-4 88-89 0.0 0.0 0.0 4.233389712941008 5.962590705911113E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8360 0.0 26.419113 1 GTACATG 4970 0.0 26.39489 1 TACCTGG 685 0.0 25.671606 2 GGTATCA 6165 0.0 25.534328 1 TACATGG 4900 0.0 25.509478 2 ACATGGG 5005 0.0 24.215843 3 GAGTACT 4910 0.0 24.042608 12-13 GTACCTG 810 0.0 22.967999 1 TATCAAC 9680 0.0 22.536123 2 GTACTTT 5205 0.0 22.407276 14-15 ATCAACG 9765 0.0 22.341068 3 TCAACGC 9810 0.0 22.190136 4 CAACGCA 9820 0.0 22.119137 5 CGATCGT 140 6.548362E-11 22.055212 24-25 AACGCAG 9995 0.0 21.918802 6 AATTCGC 130 6.2937033E-10 21.9149 78-79 ATTCGCG 135 1.0059011E-9 21.104286 80-81 CAATTCG 135 1.0077201E-9 21.103235 78-79 AGTACTT 5020 0.0 21.055374 12-13 ACGCGTA 115 0.0037127882 20.661926 6 >>END_MODULE