FastQCFastQC Report
Thu 26 May 2016
SRR938266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006274
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69710.6927536635151063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59240.5887064556969573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36990.3675937170194201No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29290.29107380296022756No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16540.16436875045961638No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.15691551207722748No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.12004682621234376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11010.10941353945346895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63100.025.4471761
GTACATG51750.024.5105531
TACATGG52050.024.3692862
GTATCAA83450.024.3082541
ACAACGC1000.001653638723.7496663
CCCATCG1201.7119267E-423.7473058
GAGTACT49700.023.07836712-13
ACATGGG51900.022.5141323
CATGGGG25550.021.750084
TAGCGCG1100.002878185621.589534
GTACTTT53600.021.26624314-15
TACCTGG6950.021.1899092
AGTACTT51500.020.7039612-13
ACGGCGA1150.003725409720.649838
GTAGCGC1404.8516213E-420.3568553
TATCAAC99400.020.3120982
TCAACGC100250.020.0411154
ATCAACG100050.020.0346983
CAACGCA100850.019.9218835
AACGCAG102150.019.8998476
Produced by FastQC (version 0.11.5)