##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938266_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1006274 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43871848025488 33.0 31.0 34.0 31.0 34.0 2 32.672842585617836 34.0 31.0 34.0 31.0 34.0 3 32.6312306588464 34.0 31.0 34.0 31.0 34.0 4 36.17071195320559 37.0 35.0 37.0 35.0 37.0 5 36.134367975322824 37.0 35.0 37.0 35.0 37.0 6 36.20362346637198 37.0 37.0 37.0 35.0 37.0 7 36.16472849343221 37.0 37.0 37.0 35.0 37.0 8 36.164446264138796 37.0 37.0 37.0 35.0 37.0 9 37.85417391287065 39.0 38.0 39.0 35.0 39.0 10-11 37.817862232354216 39.0 38.0 39.0 35.0 39.0 12-13 37.75067725092768 39.0 38.0 39.0 35.0 39.0 14-15 38.98287295507983 40.0 38.0 41.0 35.5 41.0 16-17 38.92307860483328 40.0 38.0 41.0 35.5 41.0 18-19 38.93269725740703 40.0 38.0 41.0 35.0 41.0 20-21 38.95648252861547 40.0 38.5 41.0 35.0 41.0 22-23 38.97104466576698 40.0 38.5 41.0 35.0 41.0 24-25 38.906658623794314 40.0 38.0 41.0 35.0 41.0 26-27 38.725133015461 40.0 38.0 41.0 35.0 41.0 28-29 38.63809757580937 40.0 38.0 41.0 34.5 41.0 30-31 38.41168011893381 40.0 38.0 41.0 34.0 41.0 32-33 38.18990851398327 40.0 37.5 41.0 34.0 41.0 34-35 37.95586341294717 40.0 37.0 41.0 33.0 41.0 36-37 37.86731894096439 40.0 37.0 41.0 33.0 41.0 38-39 37.81533359701234 40.0 37.0 41.0 33.0 41.0 40-41 37.68989957009721 40.0 37.0 41.0 33.0 41.0 42-43 37.485287804315725 40.0 37.0 41.0 33.0 41.0 44-45 37.19409276201114 40.0 36.5 41.0 32.0 41.0 46-47 36.77567491557966 40.0 36.0 41.0 31.0 41.0 48-49 36.34575374102879 39.0 35.5 41.0 30.0 41.0 50-51 36.03094187070321 39.0 35.0 41.0 29.5 41.0 52-53 35.56241093380133 39.0 35.0 40.0 28.0 41.0 54-55 35.2451136569165 38.0 35.0 40.0 27.0 41.0 56-57 34.962044135096406 38.0 34.0 40.0 26.0 41.0 58-59 34.577265237897436 38.0 34.0 40.0 26.0 41.0 60-61 34.14087912437368 37.0 34.0 40.0 25.0 41.0 62-63 33.710572368957166 37.0 33.0 39.5 23.5 41.0 64-65 33.2691468725218 36.0 32.5 39.0 22.5 40.5 66-67 32.72943601842043 35.5 32.0 39.0 21.5 40.0 68-69 32.18513744765342 35.0 31.0 38.5 19.5 40.0 70-71 31.57246634614429 35.0 31.0 37.5 17.5 39.5 72-73 31.088623476309632 35.0 30.0 37.0 14.5 39.0 74-75 30.516631156126465 34.0 29.5 36.5 9.5 39.0 76-77 29.3253239177401 33.0 28.5 35.0 8.0 37.0 78-79 30.025234180749976 34.0 30.0 35.5 7.5 37.5 80-81 29.820721791480253 34.0 30.0 35.0 4.5 37.0 82-83 29.34362310861654 34.0 29.0 35.0 2.0 37.0 84-85 28.814791001258108 34.0 29.0 35.0 2.0 36.0 86-87 28.25261111784663 33.5 27.5 35.0 2.0 36.0 88-89 27.60496047796127 33.0 26.5 35.0 2.0 35.0 90-91 27.02615838230939 33.0 25.5 35.0 2.0 35.0 92-93 26.351319819452755 32.0 24.5 34.5 2.0 35.0 94-95 25.561859394161033 32.0 21.5 34.0 2.0 35.0 96-97 24.689574608903737 31.5 18.0 34.0 2.0 35.0 98-99 23.614543851873346 31.0 2.0 34.0 2.0 35.0 100-101 20.185922025213806 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1146398551291199 1101 2 0.1288176225234352 1101 3 0.31944948123260275 1101 4 0.1610207412445206 1101 5 0.06490635511583065 1101 6 0.1753338526439947 1101 7 0.12342725203308902 1101 8 0.11497971548207175 1101 9 0.07763893392372267 1101 10-11 0.17992606683197465 1101 12-13 0.20958755750270086 1101 14-15 0.21717962393436352 1101 16-17 0.3124851442437162 1101 18-19 0.3678338744717493 1101 20-21 0.2965817646054205 1101 22-23 0.2125683681138355 1101 24-25 0.13889544038489277 1101 26-27 0.2307887073817554 1101 28-29 0.2769810667700696 1101 30-31 0.3482965462530032 1101 32-33 0.2898448599412973 1101 34-35 0.28918277032517636 1101 36-37 0.44545952314698667 1101 38-39 0.5725253303523132 1101 40-41 0.400639100970821 1101 42-43 0.45386641405045935 1101 44-45 0.5097389962636569 1101 46-47 0.5147098750000367 1101 48-49 0.6899858444431786 1101 50-51 0.6901381110263856 1101 52-53 0.4220106396581471 1101 54-55 0.6075975439535668 1101 56-57 0.6378813529542668 1101 58-59 0.6431939607077837 1101 60-61 0.8407013519812665 1101 62-63 1.061471856226639 1101 64-65 1.0072380707812272 1101 66-67 0.9061929930300607 1101 68-69 0.956470229501889 1101 70-71 1.0519769654159 1101 72-73 1.094023223442921 1101 74-75 1.142489620037555 1101 76-77 1.3611952284501108 1101 78-79 1.4018131279428658 1101 80-81 1.5443475036879413 1101 82-83 1.6885608330762132 1101 84-85 1.855024133991435 1101 86-87 2.0848915690343475 1101 88-89 2.1099002178669295 1101 90-91 2.0938830515709483 1101 92-93 2.281756033272032 1101 94-95 2.3539596138272607 1101 96-97 2.52946162694775 1101 98-99 2.711035498887245 1101 100-101 2.8601942855478235 1102 1 0.13975415522343582 1102 2 0.18040386675556164 1102 3 0.2002728648537513 1102 4 0.057902177894142426 1102 5 0.06161586581415435 1102 6 0.09785826697495992 1102 7 0.12106829263976238 1102 8 0.11159617892506901 1102 9 0.057143161229710415 1102 10-11 0.16707285733607335 1102 12-13 0.11865818459891386 1102 14-15 0.31768281926169806 1102 16-17 0.3009345963222714 1102 18-19 0.42970025438369674 1102 20-21 0.31688034055947156 1102 22-23 0.2781487080746672 1102 24-25 0.1773905702069598 1102 26-27 0.29454035156906144 1102 28-29 0.11158428366745454 1102 30-31 0.28428373533770923 1102 32-33 0.39055846894827084 1102 34-35 0.10544880511638866 1102 36-37 0.29711312090369546 1102 38-39 0.4282259550377532 1102 40-41 0.41645713437478804 1102 42-43 0.18256758010174678 1102 44-45 0.30087064121224927 1102 46-47 0.38386322956506547 1102 48-49 0.5147123066596464 1102 50-51 0.3728036188317745 1102 52-53 0.393212514137403 1102 54-55 0.38162171086343477 1102 56-57 0.3253033730101791 1102 58-59 0.5930742681020789 1102 60-61 0.7471624265078844 1102 62-63 0.5884111513513872 1102 64-65 0.6533883463279864 1102 66-67 0.6855837661258661 1102 68-69 0.6736821742318213 1102 70-71 1.0677801958474298 1102 72-73 0.9859086568951021 1102 74-75 1.0868119399593965 1102 76-77 1.07889937201357 1102 78-79 1.203544758511427 1102 80-81 1.33174562751692 1102 82-83 1.1520381993313542 1102 84-85 1.2890747770748519 1102 86-87 1.2844694728488655 1102 88-89 1.417024359823209 1102 90-91 1.579391998910431 1102 92-93 1.778048417354185 1102 94-95 1.8047243988246606 1102 96-97 2.193177215323608 1102 98-99 2.367452693174144 1102 100-101 2.582677145820451 1103 1 0.24770424059945384 1103 2 0.17525163308692981 1103 3 0.24380384434461888 1103 4 0.15999193466016237 1103 5 0.03648861103754797 1103 6 0.0789905714800625 1103 7 0.05913089093007784 1103 8 0.01308735943398176 1103 9 0.10638071157188733 1103 10-11 0.09107606197146367 1103 12-13 0.11588514993327692 1103 14-15 0.2685997010543488 1103 16-17 0.25424821089742267 1103 18-19 0.1904619487402428 1103 20-21 0.17767359571912067 1103 22-23 0.28470634160788677 1103 24-25 0.30856751841217545 1103 26-27 0.3635007063448583 1103 28-29 0.23108171159687174 1103 30-31 0.2869286385377592 1103 32-33 0.4416667386879354 1103 34-35 0.24897425472295964 1103 36-37 0.12147050788500735 1103 38-39 0.16988931726135803 1103 40-41 0.5054963641832941 1103 42-43 0.4072663362824045 1103 44-45 0.4114069590645997 1103 46-47 0.24488712136276547 1103 48-49 0.4756052346119972 1103 50-51 0.5982178880912912 1103 52-53 0.7642954003593587 1103 54-55 0.8669702149622864 1103 56-57 0.9640737964914123 1103 58-59 0.9672842364603511 1103 60-61 0.9579042942060738 1103 62-63 0.8324611307154797 1103 64-65 0.8499076213466594 1103 66-67 0.9377984173520204 1103 68-69 0.9867633178658437 1103 70-71 0.9167091516703998 1103 72-73 0.7771588962986264 1103 74-75 1.0455347255104819 1103 76-77 0.5357521125433031 1103 78-79 0.9807841593417201 1103 80-81 1.1761603094946231 1103 82-83 1.455187805630569 1103 84-85 1.5635964420825559 1103 86-87 1.5097562850189234 1103 88-89 1.58407879276729 1103 90-91 1.8097056107880825 1103 92-93 2.0810698921878164 1103 94-95 1.8372030278449536 1103 96-97 1.6377212426439343 1103 98-99 2.049322495420089 1103 100-101 2.091922842626335 1104 1 0.19864655323264202 1104 2 0.17156147535472854 1104 3 0.27806815535564766 1104 4 0.188462823975577 1104 5 0.041763143659544255 1104 6 0.03918976443123512 1104 7 0.031614656754939574 1104 8 0.1116662079032622 1104 9 0.18899490495126514 1104 10-11 0.18478969565592962 1104 12-13 0.12158411366645794 1104 14-15 0.19632802484112943 1104 16-17 0.12562543317842056 1104 18-19 0.21401346737238924 1104 20-21 0.182892713003163 1104 22-23 0.19162494655194706 1104 24-25 0.27507902213255875 1104 26-27 0.3014456033478936 1104 28-29 0.32143893901638876 1104 30-31 0.16220004774979913 1104 32-33 0.2198869995430499 1104 34-35 0.22956629204266932 1104 36-37 0.35680937267661506 1104 38-39 0.4273905389176704 1104 40-41 0.33621132279810695 1104 42-43 0.35821998387108067 1104 44-45 0.13207713201083493 1104 46-47 0.2220371105315877 1104 48-49 0.3215783027577501 1104 50-51 0.4841378582677649 1104 52-53 0.5708203045160118 1104 54-55 0.5637261507006528 1104 56-57 0.673691128394033 1104 58-59 0.5346025371238241 1104 60-61 0.6756953884574912 1104 62-63 0.7719664732578337 1104 64-65 1.008001283343333 1104 66-67 0.7390198217781929 1104 68-69 0.8982222022039608 1104 70-71 1.1300556839296796 1104 72-73 1.0045960376732275 1104 74-75 0.9671472110899018 1104 76-77 0.9176532112783242 1104 78-79 1.1167280588686737 1104 80-81 1.296925444179081 1104 82-83 1.2667215006749224 1104 84-85 1.3619236545399254 1104 86-87 1.5851324795972879 1104 88-89 1.6998477309590356 1104 90-91 1.8290216343849401 1104 92-93 2.022134420829989 1104 94-95 2.4552931130334414 1104 96-97 2.611197446794037 1104 98-99 2.895368611056785 1104 100-101 2.919030766515892 1105 1 0.07653078434031357 1105 2 0.08880655636858137 1105 3 0.1841311563717909 1105 4 0.0479471167329919 1105 5 0.05840498913734393 1105 6 0.07263474831090377 1105 7 0.04477974364701964 1105 8 0.023545610976071885 1105 9 7.98321135398794E-4 1105 10-11 0.06684379688694975 1105 12-13 0.09245057925461708 1105 14-15 -0.026824767945846872 1105 16-17 0.10648175523379422 1105 18-19 0.047481659511689145 1105 20-21 0.13019607452691417 1105 22-23 0.18843896326547593 1105 24-25 0.11239718676245758 1105 26-27 0.12294989284360014 1105 28-29 0.07326871763288523 1105 30-31 0.027883813258569035 1105 32-33 0.052627133344486765 1105 34-35 -0.014310849344916221 1105 36-37 0.08293488366155799 1105 38-39 0.038514195033990006 1105 40-41 -0.049684238241404444 1105 42-43 -0.0871259088046088 1105 44-45 -0.06744311114720603 1105 46-47 -0.1342768801958698 1105 48-49 -0.16582561594344014 1105 50-51 -0.16689235548120251 1105 52-53 -0.14711824646420268 1105 54-55 0.12620251680691297 1105 56-57 0.36365252562086425 1105 58-59 0.4005425791675137 1105 60-61 0.2862437664779236 1105 62-63 0.27060489215705985 1105 64-65 0.3785896978518082 1105 66-67 0.4943115737956987 1105 68-69 0.5660640670368977 1105 70-71 0.4350561230048662 1105 72-73 0.4881858267558492 1105 74-75 0.6637184819526922 1105 76-77 0.6873269156929105 1105 78-79 0.911526272459934 1105 80-81 0.8699302059046303 1105 82-83 0.8233734000783208 1105 84-85 1.0299039624660367 1105 86-87 1.2643880589883558 1105 88-89 1.262885486048276 1105 90-91 1.2377862918794165 1105 92-93 1.292826510845778 1105 94-95 1.1315688056521367 1105 96-97 1.3256628234757422 1105 98-99 1.3391392095072945 1105 100-101 1.5190238667630247 1106 1 0.02399239543778009 1106 2 0.0809514507699447 1106 3 0.11177987844309456 1106 4 0.021757564569050203 1106 5 -0.047201563882865116 1106 6 0.030985792152883107 1106 7 0.010063626600135933 1106 8 0.03863276427911444 1106 9 -0.040996719218163946 1106 10-11 0.055939843725418825 1106 12-13 0.029841181112701065 1106 14-15 0.08878679241816911 1106 16-17 -0.08312541028400489 1106 18-19 -0.09263464462856774 1106 20-21 0.03484357600316201 1106 22-23 0.044388066123708825 1106 24-25 0.011244881668254436 1106 26-27 0.01750042433486243 1106 28-29 -0.09942348208709006 1106 30-31 0.07796913170891884 1106 32-33 0.18993571684700328 1106 34-35 -0.12911146641864235 1106 36-37 0.07566367483244107 1106 38-39 0.08110873834927901 1106 40-41 -0.01190167312613255 1106 42-43 0.0719954211599827 1106 44-45 0.29779465848152853 1106 46-47 -0.1572288193755611 1106 48-49 0.09039814280290415 1106 50-51 0.38833319059647664 1106 52-53 0.23272574265516255 1106 54-55 0.37637302170380593 1106 56-57 0.2935690728240985 1106 58-59 0.4738112834446966 1106 60-61 0.408382474301618 1106 62-63 0.5368367383356869 1106 64-65 0.7698916160428979 1106 66-67 0.655394480096767 1106 68-69 0.3871043273131747 1106 70-71 0.5915738981953673 1106 72-73 0.544167314544179 1106 74-75 0.7013774035169185 1106 76-77 0.8131516093781137 1106 78-79 1.1634097465533095 1106 80-81 1.1926183135070794 1106 82-83 1.0714538449486959 1106 84-85 1.0623601746084503 1106 86-87 1.506048719482525 1106 88-89 1.6188816702586877 1106 90-91 1.4535610089292916 1106 92-93 1.5880063484107119 1106 94-95 1.7878692145998833 1106 96-97 1.7168120572817358 1106 98-99 2.187246407146013 1106 100-101 2.718203947214935 1107 1 0.17250047039543404 1107 2 0.13721938434281356 1107 3 0.21444078253492904 1107 4 0.1680092026219242 1107 5 0.079726499548471 1107 6 0.06448446383695483 1107 7 0.07885356435630797 1107 8 0.08366821074061193 1107 9 0.136177172028205 1107 10-11 0.0941972606282988 1107 12-13 0.07018283774542766 1107 14-15 0.1705259577283229 1107 16-17 0.20701200742100667 1107 18-19 0.16604169530522483 1107 20-21 0.14145324467835252 1107 22-23 0.06664248025660413 1107 24-25 0.22000365827489787 1107 26-27 0.19499604093589795 1107 28-29 0.20252535399078653 1107 30-31 0.13922468016345846 1107 32-33 0.1630023349206482 1107 34-35 0.08356468693187935 1107 36-37 0.12013631989439233 1107 38-39 0.12112954151177036 1107 40-41 0.30222390090077766 1107 42-43 -0.07077783395949666 1107 44-45 -0.12573846135637012 1107 46-47 -0.1547653099186661 1107 48-49 0.11691868745167255 1107 50-51 0.06567535553782733 1107 52-53 0.23214681139366178 1107 54-55 0.22478632282392397 1107 56-57 0.3528833705953005 1107 58-59 0.27405035415539913 1107 60-61 0.25470892003858125 1107 62-63 0.15592220131939172 1107 64-65 0.25863471616749223 1107 66-67 0.3153948637259205 1107 68-69 0.5215462907620392 1107 70-71 0.6819891854682076 1107 72-73 0.600677631988578 1107 74-75 0.9292539900196246 1107 76-77 0.8186867676818075 1107 78-79 0.9524621040852139 1107 80-81 0.8806868366481986 1107 82-83 1.0327042492851 1107 84-85 1.3000833879210774 1107 86-87 1.307031943554204 1107 88-89 1.5230964467816293 1107 90-91 1.5949213753300064 1107 92-93 1.906438159637272 1107 94-95 2.1460602636599475 1107 96-97 2.037032539416387 1107 98-99 2.142708370751812 1107 100-101 2.3600691347006055 1108 1 0.055039893410295804 1108 2 0.03014023712770353 1108 3 0.198741279490811 1108 4 0.06200743853612778 1108 5 0.010237008766900146 1108 6 -0.023294724253318577 1108 7 0.03560162278627388 1108 8 -0.02929437773259025 1108 9 -0.004282781072923569 1108 10-11 -0.0048514238369889995 1108 12-13 0.061354233739663755 1108 14-15 0.1179514681299807 1108 16-17 0.16217410969495205 1108 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5663.0 16 7817.0 17 10981.0 18 11085.0 19 7865.0 20 6762.0 21 6954.0 22 7443.0 23 7977.0 24 9022.0 25 10465.0 26 12423.0 27 14789.0 28 17917.0 29 21831.0 30 26556.0 31 32527.0 32 40834.0 33 52741.0 34 73063.0 35 107329.0 36 154899.0 37 210046.0 38 129427.0 39 7381.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.068812271806685 18.95338645339142 14.890179016848293 22.087622257953598 2 15.831723277040997 20.536864325923098 39.951062239608184 23.680350157427725 3 20.16141695504137 24.773656498518207 29.705013048914047 25.35991349752637 4 12.989814891187581 17.212327072275617 36.51851932825386 33.27933870828294 5 13.5326502827273 37.70469158376976 33.5947608494738 15.167897284029136 6 31.452330980092857 36.078416173758185 17.211747917064173 15.257504929084782 7 28.320318322842486 33.3238263137078 20.170748722514944 18.185106640934777 8 26.39952219678178 34.53002766647587 19.209120396870826 19.861329739871525 9 26.322154800779906 17.28823362225398 20.355986540445247 36.03362503652087 10-11 25.276713897010154 26.700133363278788 26.353011207682997 21.670141532028055 12-13 26.420090353124497 24.287172281108326 27.24059252251375 22.052144843253426 14-15 22.983551199772627 25.520583856881924 26.427096397203943 25.068768546141506 16-17 21.725543937337147 28.983308721083915 26.927159004406352 22.36398833717258 18-19 21.531560986371506 27.094061855915985 29.882318334767668 21.49205882294484 20-21 22.506842072835035 25.78452787213025 30.157293142821935 21.551336912212776 22-23 22.14222965116857 25.891506687045478 30.173342449472013 21.792921212313942 24-25 22.133510624025472 26.088066964999996 30.19397629035127 21.58444612062326 26-27 21.762191079307744 26.312692399827842 30.352281164920385 21.57283535594403 28-29 21.39881154980008 26.838612159677066 30.249878635771665 21.512697654751193 30-31 21.674386573027398 26.15577482452575 30.15036674938113 22.01947185306572 32-33 21.501924748232394 26.287235940294295 30.212261443767392 21.998577867705922 34-35 22.16267444189279 26.29033949357992 29.90254959365432 21.644436470872968 36-37 22.070570766804995 25.952325698693524 30.081650278801586 21.89545325569989 38-39 21.58228115471685 26.452627733779966 30.083768410537274 21.881322700965907 40-41 21.890720844031335 26.33254110964992 29.929703815695174 21.84703423062357 42-43 21.742028050606592 26.35296889047481 29.659682260110955 22.245320798807644 44-45 21.806839030537706 26.36453626936311 29.57310225151715 22.25552244858204 46-47 22.231066947114012 26.82957854885891 29.047120023413676 21.892234480613407 48-49 22.095831799207673 26.773089025995418 28.994155167556336 22.136924007240577 50-51 21.883353838020263 27.07249715286294 28.978985842822137 22.065163166294667 52-53 22.279269860892757 27.221164414463654 28.488562757260944 22.01100296738264 54-55 21.822535412819967 26.96363018422418 28.726221685147387 22.48761271780847 56-57 22.108690078447818 27.090235860213024 28.55141840095242 22.249655660386733 58-59 22.461178565678928 26.748480036252552 28.474848798637346 22.31549259943117 60-61 22.056517409771097 26.861769259664865 29.05714546932545 22.02456786123859 62-63 21.75530720261082 27.150954908901554 28.917223340760074 22.176514547727557 64-65 21.62502459568666 27.21689122445775 28.615218121505677 22.54286605834991 66-67 21.69250545205742 27.15628933145776 28.412361872516513 22.738843343968306 68-69 21.71112780639827 27.007161085670674 28.60315103669783 22.678560071233225 70-71 21.886484198140867 27.08531672288065 28.41353349087823 22.61466558810026 72-73 21.9511783073 26.863707101644284 28.600659462532075 22.584455128523643 74-75 21.672228438775125 27.631837849333284 28.05359176526473 22.64234194662686 76-77 21.747158328646073 27.158855341586886 28.29507668885413 22.79890964091291 78-79 21.879875660108482 27.274927107328622 28.30238086246887 22.54281637009403 80-81 21.90077737392207 27.418865168232266 28.310870067501597 22.369487390344066 82-83 21.875863343741493 27.30029554927047 28.395984346025188 22.42785676096285 84-85 21.830011707018446 27.318595873325684 28.267778667785027 22.58361375187084 86-87 21.87424690601039 27.274809679950273 28.2412047317532 22.60973868228614 88-89 21.746065187016658 27.290777660955168 27.957097172340735 23.00605997968744 90-91 21.786247388788734 27.230138396681504 28.155366821765824 22.828247392763938 92-93 22.041190713630183 27.033138703758087 28.173637207887936 22.752033374723794 94-95 21.757018224676603 27.0313821398368 28.127605530507125 23.083994104979464 96-97 21.589210090497264 27.00796552825847 28.3416983553676 23.061126025876664 98-99 21.69803820633168 27.092300002086912 27.842543723224217 23.367118068357197 100-101 21.735703999928447 26.66269479638616 28.07771085115623 23.52389035252916 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1238.0 1 843.0 2 741.0 3 959.5 4 659.0 5 498.0 6 980.0 7 2126.0 8 3304.0 9 3655.0 10 2820.5 11 1862.0 12 1688.0 13 1799.5 14 1854.0 15 1875.5 16 1890.0 17 1934.5 18 2017.0 19 2197.0 20 2416.5 21 2665.0 22 3104.0 23 3563.0 24 4161.0 25 5009.5 26 5993.0 27 7243.0 28 8333.5 29 9474.0 30 11163.5 31 12759.0 32 14448.0 33 15937.5 34 17271.5 35 18603.5 36 20147.0 37 22237.5 38 24059.0 39 25181.5 40 26381.0 41 28726.5 42 30781.0 43 32461.0 44 34318.5 45 36180.5 46 36922.0 47 39049.0 48 44049.0 49 44156.0 50 40296.0 51 37120.0 52 34975.5 53 34434.5 54 33474.0 55 30760.5 56 27468.5 57 24876.5 58 23076.0 59 20942.5 60 17636.0 61 14118.5 62 11052.0 63 8279.5 64 6335.0 65 5034.5 66 3735.5 67 3026.5 68 2382.0 69 1609.0 70 1169.5 71 879.0 72 658.0 73 538.0 74 421.0 75 354.0 76 253.5 77 120.0 78 55.5 79 31.5 80 16.5 81 9.5 82 8.0 83 3.5 84 1.0 85 1.0 86 1.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.009738898152988153 3 0.005962590705911114 4 9.937651176518523E-5 5 3.9750604706074094E-4 6 1.9875302353037047E-4 7 0.0 8 1.9875302353037047E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016894007000081489 26-27 0.022955974217757788 28-29 5.465708147085188E-4 30-31 9.937651176518523E-5 32-33 0.003825995702959631 34-35 0.017142448279494453 36-37 0.009192327338279634 38-39 0.022508779914814453 40-41 0.024347245382470382 42-43 0.019825614097154453 44-45 0.010732663270640005 46-47 0.002434724538247038 48-49 4.968825588259262E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.4844127941296305E-4 68-69 1.9875302353037047E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4906476764777783E-4 82-83 0.0011925181411822226 84-85 0.003975060470607409 86-87 8.447003500040744E-4 88-89 0.0 90-91 0.003577554423546668 92-93 3.4781779117814827E-4 94-95 9.937651176518523E-5 96-97 4.968825588259262E-5 98-99 9.937651176518523E-5 100-101 0.003080671864720742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1006274.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.408637301032535 #Duplication Level Percentage of deduplicated Percentage of total 1 83.25502627726897 51.958327384260116 2 9.995365831927595 12.475943217918052 3 2.728404844441245 5.108280850413413 4 1.1736592483514223 2.9298589738146554 5 0.6872219377587988 2.1444292329450816 6 0.41318745646369626 1.5471879664667394 7 0.2945917459186729 1.2869548596041473 8 0.22288119659119032 1.1127769407423782 9 0.17932944895301609 1.007253588339253 >10 0.95136253768171 10.883751875780188 >50 0.0651903885697171 2.7555410283446893 >100 0.03075171556611971 3.4986614947837382 >500 0.001752688188749624 0.7985944737395541 >1k 9.560117393179768E-4 1.210172425029308 >5k 3.18670579772659E-4 1.2822656878186465 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6971 0.6927536635151063 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5924 0.5887064556969573 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3699 0.3675937170194201 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2929 0.29107380296022756 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1654 0.16436875045961638 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1579 0.15691551207722748 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1208 0.12004682621234376 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1101 0.10941353945346895 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.968825588259262E-5 0.0 12-13 0.0 0.0 0.0 2.4844127941296305E-4 0.0 14-15 0.0 0.0 0.0 2.9812953529555566E-4 0.0 16-17 0.0 0.0 0.0 2.9812953529555566E-4 0.0 18-19 0.0 0.0 0.0 2.9812953529555566E-4 0.0 20-21 0.0 0.0 0.0 3.478177911781483E-4 0.0 22-23 0.0 0.0 0.0 6.45947326473704E-4 4.968825588259262E-5 24-25 0.0 0.0 0.0 8.943886058866671E-4 9.937651176518523E-5 26-27 0.0 0.0 0.0 0.002335348026481853 9.937651176518523E-5 28-29 0.0 0.0 0.0 0.0060619672176762986 9.937651176518523E-5 30-31 0.0 0.0 0.0 0.016595877464785934 9.937651176518523E-5 32-33 0.0 0.0 0.0 0.036769309353118534 9.937651176518523E-5 34-35 0.0 0.0 0.0 0.06216000810912336 9.937651176518523E-5 36-37 0.0 0.0 0.0 0.10504097293580078 9.937651176518523E-5 38-39 0.0 0.0 0.0 0.16799599313904562 9.937651176518523E-5 40-41 0.0 0.0 0.0 0.23522420334819344 9.937651176518523E-5 42-43 0.0 0.0 0.0 0.30364493169852347 9.937651176518523E-5 44-45 0.0 0.0 0.0 0.3842392827400887 9.937651176518523E-5 46-47 0.0 0.0 0.0 0.4797401105464317 9.937651176518523E-5 48-49 0.0 0.0 0.0 0.5803588287086817 9.937651176518523E-5 50-51 0.0 0.0 0.0 0.6858966842033085 9.937651176518523E-5 52-53 0.0 0.0 0.0 0.7890991916714534 9.937651176518523E-5 54-55 0.0 0.0 0.0 0.898910237171983 9.937651176518523E-5 56-57 0.0 0.0 0.0 1.0273046903726022 9.937651176518523E-5 58-59 0.0 0.0 0.0 1.167226818937983 9.937651176518523E-5 60-61 0.0 0.0 0.0 1.311422137509267 9.937651176518523E-5 62-63 0.0 0.0 0.0 1.4555180795687854 9.937651176518523E-5 64-65 0.0 0.0 0.0 1.5945955077841623 9.937651176518523E-5 66-67 0.0 0.0 0.0 1.759262387779074 9.937651176518523E-5 68-69 0.0 0.0 0.0 1.9458914768740918 9.937651176518523E-5 70-71 0.0 0.0 0.0 2.1351540435308873 9.937651176518523E-5 72-73 0.0 0.0 0.0 2.3309257717083023 1.9875302353037047E-4 74-75 0.0 0.0 0.0 2.524511216626883 2.9812953529555566E-4 76-77 0.0 0.0 0.0 2.726891482836683 2.9812953529555566E-4 78-79 0.0 0.0 0.0 2.9660907466554836 3.478177911781483E-4 80-81 0.0 0.0 0.0 3.221190252356714 4.4719430294333355E-4 82-83 0.0 0.0 0.0 3.4771344584079484 4.968825588259261E-4 84-85 0.0 0.0 0.0 3.728457656662102 4.968825588259261E-4 86-87 0.0 0.0 0.0 3.9913085302810165 4.968825588259261E-4 88-89 0.0 0.0 0.0 4.280643244285354 4.968825588259261E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6310 0.0 25.447176 1 GTACATG 5175 0.0 24.510553 1 TACATGG 5205 0.0 24.369286 2 GTATCAA 8345 0.0 24.308254 1 ACAACGC 100 0.0016536387 23.749666 3 CCCATCG 120 1.7119267E-4 23.747305 8 GAGTACT 4970 0.0 23.078367 12-13 ACATGGG 5190 0.0 22.514132 3 CATGGGG 2555 0.0 21.75008 4 TAGCGCG 110 0.0028781856 21.58953 4 GTACTTT 5360 0.0 21.266243 14-15 TACCTGG 695 0.0 21.189909 2 AGTACTT 5150 0.0 20.70396 12-13 ACGGCGA 115 0.0037254097 20.64983 8 GTAGCGC 140 4.8516213E-4 20.356855 3 TATCAAC 9940 0.0 20.312098 2 TCAACGC 10025 0.0 20.041115 4 ATCAACG 10005 0.0 20.034698 3 CAACGCA 10085 0.0 19.921883 5 AACGCAG 10215 0.0 19.899847 6 >>END_MODULE