FastQCFastQC Report
Thu 26 May 2016
SRR938265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035088
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72270.6982015055724731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62530.6041032260059048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38220.36924396766265283No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30650.2961100891904843No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17270.16684571746556814No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.1552525002705084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12140.11728471395668774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.11419318937133846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64050.026.5411551
GTATCAA87000.025.3578051
GTACATG53850.025.1838191
TACGGTG950.001223011425.0121175
TACATGG53750.024.4909362
GAGTACT48800.023.81596412-13
ACATGGG55100.023.1146493
GTCCTAC2154.1798558E-822.21011
GTACTTT51850.022.1250114-15
ATCAACG100250.021.7112673
TCAACGC100200.021.6272474
TATCAAC101800.021.5674252
CAACGCA100850.021.4878545
AGTACTT50800.021.33439412-13
AACGCAG102450.021.291436
AATTCGC1159.075302E-820.64221478-79
AGAGTAC80500.020.22529410-11
CATGGGG28400.019.7454834
ACGCAGA113300.019.1778777
CGCAGAG114100.018.9184028
Produced by FastQC (version 0.11.5)