FastQCFastQC Report
Thu 26 May 2016
SRR938265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035088
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72110.6966557432797984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62630.6050693274388265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39470.38132023557417344No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31420.303549070223981No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17330.16742537832532114No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17230.16645927689239948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12180.1176711545298564No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11910.11506268066096795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT352.0549237E-433.9256972-73
CGTATAG600.00395148131.6655063
CGTAGAC1056.906905E-527.1418633
TAGACTA2305.456968E-1226.8455475
GGTATCA66000.024.112461
GTATCAA86750.023.8210261
TACATGG52450.023.6393572
GTACATG53050.023.551091
CGTAAGA1252.2540383E-422.8024712
GAGTACT49350.022.76149212-13
ACATGGG53050.022.2046533
TACCTGG6250.022.0423872
AGTACTT49400.021.77699712-13
CCTAGAC3750.021.5325453
GTCTATA2951.3278623E-1020.9344711
GTACTTT53250.020.91606514-15
CTAGACT3650.020.8201484
GTATAGG2108.597035E-720.3593481
CCGTTCA1404.8540687E-420.3554159
ATCAACG101300.020.2559223
Produced by FastQC (version 0.11.5)