##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938265_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1035088 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45651963890993 33.0 31.0 34.0 31.0 34.0 2 32.676099036990095 34.0 31.0 34.0 31.0 34.0 3 32.72804437883542 34.0 31.0 34.0 31.0 34.0 4 36.198543505479726 37.0 35.0 37.0 35.0 37.0 5 36.1654410059821 37.0 35.0 37.0 35.0 37.0 6 36.21176750189356 37.0 37.0 37.0 35.0 37.0 7 36.17985620546273 37.0 37.0 37.0 35.0 37.0 8 36.17931905306602 37.0 37.0 37.0 35.0 37.0 9 37.87073949268081 39.0 38.0 39.0 35.0 39.0 10-11 37.855886166200364 39.0 38.0 39.0 35.0 39.0 12-13 37.80107874886 39.0 38.0 39.0 35.0 39.0 14-15 39.07774846196652 40.0 38.0 41.0 36.0 41.0 16-17 39.03837306586493 40.0 38.0 41.0 36.0 41.0 18-19 39.08252776575518 40.0 38.5 41.0 36.0 41.0 20-21 39.02335356993801 40.0 38.5 41.0 35.0 41.0 22-23 38.87123317051304 40.0 38.0 41.0 35.0 41.0 24-25 38.81613157528635 40.0 38.0 41.0 35.0 41.0 26-27 38.78008391557046 40.0 38.0 41.0 35.0 41.0 28-29 38.71921807614425 40.0 38.0 41.0 35.0 41.0 30-31 38.51354667429243 40.0 38.0 41.0 34.0 41.0 32-33 38.319702769233146 40.0 38.0 41.0 34.0 41.0 34-35 38.10061415068091 40.0 37.5 41.0 34.0 41.0 36-37 37.962779010093826 40.0 37.0 41.0 33.0 41.0 38-39 37.94812470050856 40.0 37.0 41.0 33.0 41.0 40-41 37.89005041117277 40.0 37.0 41.0 33.0 41.0 42-43 37.713412289583104 40.0 37.0 41.0 33.0 41.0 44-45 37.473450566521876 40.0 37.0 41.0 33.0 41.0 46-47 37.08398802807105 40.0 37.0 41.0 31.5 41.0 48-49 36.70015158131483 40.0 36.0 41.0 31.0 41.0 50-51 36.38886790301888 39.5 36.0 41.0 30.0 41.0 52-53 35.98508774133214 39.0 35.0 41.0 29.0 41.0 54-55 35.76605225835871 39.0 35.0 41.0 28.5 41.0 56-57 35.52653107755089 39.0 35.0 40.0 28.0 41.0 58-59 35.19232664275888 38.0 35.0 40.0 27.5 41.0 60-61 34.82693403845856 38.0 34.0 40.0 26.0 41.0 62-63 34.47185698220827 37.5 34.0 40.0 26.0 41.0 64-65 34.08843306076392 37.0 34.0 40.0 26.0 41.0 66-67 33.65528438161779 36.0 33.0 39.0 25.0 41.0 68-69 33.20492798679919 36.0 33.0 39.0 23.5 40.0 70-71 32.67275052942358 35.0 32.0 38.5 22.5 40.0 72-73 32.25527877822948 35.0 32.0 37.5 21.5 39.5 74-75 31.814015813148252 35.0 31.5 37.0 20.0 39.0 76-77 30.514586682484968 33.5 30.0 35.5 19.5 38.0 78-79 31.24487386579692 34.5 31.0 36.0 20.0 38.5 80-81 31.065087219637363 35.0 31.0 36.0 18.5 37.0 82-83 30.69587658247415 34.5 31.0 35.0 17.5 37.0 84-85 30.305589959501027 34.0 31.0 35.0 13.0 36.5 86-87 29.867073620793597 34.0 30.0 35.0 7.0 36.0 88-89 29.3955682995069 34.0 30.0 35.0 4.0 36.0 90-91 29.04014731114649 34.0 29.0 35.0 2.0 35.0 92-93 28.606668708361028 34.0 29.0 35.0 2.0 35.0 94-95 28.172903656500704 34.0 29.0 35.0 2.0 35.0 96-97 27.569895506469017 33.5 27.5 35.0 2.0 35.0 98-99 26.865489214443606 33.0 26.5 35.0 2.0 35.0 100-101 24.42698495200408 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14501774058319228 1101 2 0.11028054319912428 1101 3 0.12757597746266924 1101 4 0.03472733545634554 1101 5 -0.01939998244461094 1101 6 -0.05663170999508793 1101 7 0.01857565002218564 1101 8 -0.003924702243956801 1101 9 0.14220840219552855 1101 10-11 0.08109932441536927 1101 12-13 0.10729325400888001 1101 14-15 0.14183491686849692 1101 16-17 0.21872691504375297 1101 18-19 0.18663668232160546 1101 20-21 0.04424792489721341 1101 22-23 0.1687987823189374 1101 24-25 0.09606598183751203 1101 26-27 0.15179621919336483 1101 28-29 0.011915744527506433 1101 30-31 -0.013394612911348247 1101 32-33 0.08403382985416386 1101 34-35 -0.1285182934502913 1101 36-37 -0.02358438946640007 1101 38-39 -0.21738384171042924 1101 40-41 -0.0029642481364007267 1101 42-43 0.10037994114990312 1101 44-45 0.01145491032973922 1101 46-47 0.07630792933557018 1101 48-49 0.11092921607755812 1101 50-51 0.1878834822325004 1101 52-53 0.2303702846263107 1101 54-55 0.04368915557331832 1101 56-57 0.18141858592808546 1101 58-59 0.3873728583905347 1101 60-61 0.32099732345057674 1101 62-63 0.34002330910820433 1101 64-65 0.4252983645566317 1101 66-67 0.17610574744622198 1101 68-69 0.13627048630653604 1101 70-71 -0.024999553270376396 1101 72-73 0.20296932788319566 1101 74-75 0.21168913930436517 1101 76-77 0.12014471679848526 1101 78-79 0.19876965511032907 1101 80-81 0.3344183101990623 1101 82-83 0.2889243855343011 1101 84-85 0.27324040037549935 1101 86-87 0.5332276914981868 1101 88-89 0.6486765578531113 1101 90-91 0.6536785165090997 1101 92-93 0.5345510378884306 1101 94-95 0.42083047092185666 1101 96-97 0.6997275398146918 1101 98-99 0.7995702848128481 1101 100-101 1.0100243902079562 1102 1 0.19461698447072706 1102 2 0.16706533180130378 1102 3 0.1352784604171191 1102 4 0.07992073845768033 1102 5 0.14598310903178913 1102 6 0.05592875423815258 1102 7 0.1554208615850925 1102 8 0.2298180544298134 1102 9 0.16204397249912716 1102 10-11 0.12311474382666177 1102 12-13 0.21353334860548046 1102 14-15 0.23256118930010672 1102 16-17 0.1907462140713676 1102 18-19 0.24006704733572093 1102 20-21 0.2202736500552831 1102 22-23 0.14035221784301655 1102 24-25 0.16178214250315648 1102 26-27 0.06372376942201896 1102 28-29 0.20380094915662994 1102 30-31 0.2111046483106449 1102 32-33 0.06663540151150471 1102 34-35 0.08082621647987054 1102 36-37 0.0025060303576154297 1102 38-39 -0.042146421572745396 1102 40-41 0.034951262899873825 1102 42-43 0.07267628192879272 1102 44-45 0.10339614239683215 1102 46-47 0.035923806046312734 1102 48-49 -0.09511452453294567 1102 50-51 -0.08986144950277009 1102 52-53 -0.16795745628136416 1102 54-55 -0.18187442060749248 1102 56-57 -0.018843907261953063 1102 58-59 -0.19823510637924358 1102 60-61 -0.16493442419276505 1102 62-63 -0.1771852656693298 1102 64-65 -0.1373543437457485 1102 66-67 -0.13549193221302858 1102 68-69 0.08228706960272802 1102 70-71 0.2126346855819179 1102 72-73 -0.023991322901807166 1102 74-75 0.15108561132985088 1102 76-77 0.04688600417074795 1102 78-79 0.1255274514882565 1102 80-81 0.15128545810324212 1102 82-83 0.19532709383668134 1102 84-85 0.26028186274321996 1102 86-87 0.40795787243688864 1102 88-89 0.09901540519433283 1102 90-91 -0.010605694277881383 1102 92-93 0.16260675165529292 1102 94-95 0.0667184903364415 1102 96-97 0.04421167466025011 1102 98-99 0.020930255426822697 1102 100-101 0.3374898038410912 1103 1 0.14592071494704584 1103 2 0.22331291150958066 1103 3 0.18206269947739884 1103 4 0.08437407523320672 1103 5 0.1663619653632722 1103 6 0.10983671861146149 1103 7 0.07879442554533966 1103 8 -0.007112370250709432 1103 9 0.08661154704411445 1103 10-11 -0.02929102614881174 1103 12-13 0.19171441869713135 1103 14-15 0.2727622348661356 1103 16-17 0.18787413455672208 1103 18-19 0.26312435984378624 1103 20-21 0.2526531195273378 1103 22-23 0.3184090462927358 1103 24-25 0.2691382158218616 1103 26-27 0.22787602190685163 1103 28-29 0.32462191211003244 1103 30-31 0.32799263720957583 1103 32-33 0.3734073777603726 1103 34-35 0.3116817089285675 1103 36-37 0.4605704423880823 1103 38-39 0.3938812342940423 1103 40-41 0.5489042446992798 1103 42-43 0.407083955867094 1103 44-45 0.39123740353017666 1103 46-47 0.5526883600672221 1103 48-49 0.6263744389367858 1103 50-51 0.7873692527045435 1103 52-53 0.6643768344118115 1103 54-55 0.9086122193821851 1103 56-57 0.8883137021352354 1103 58-59 0.8618244327972064 1103 60-61 0.6579123229351538 1103 62-63 0.6313212529830494 1103 64-65 0.5537680262039046 1103 66-67 0.6830386280038354 1103 68-69 0.6221331390489624 1103 70-71 0.5012907782629554 1103 72-73 0.6431217531276303 1103 74-75 0.4070879348099119 1103 76-77 0.5464422840210084 1103 78-79 0.5652379845978466 1103 80-81 0.5955905116723663 1103 82-83 0.5128169286114321 1103 84-85 0.5613016123237031 1103 86-87 0.4302608606904066 1103 88-89 0.507221807672714 1103 90-91 0.3271298807834313 1103 92-93 0.3973871505193678 1103 94-95 0.3171138046516617 1103 96-97 0.20870276798669707 1103 98-99 0.5661816430607409 1103 100-101 0.6635999109793396 1104 1 0.06401181556620372 1104 2 -0.03632025332941424 1104 3 0.030094763924587653 1104 4 0.04712596416335657 1104 5 0.02628652183413749 1104 6 0.06296775769355634 1104 7 0.01700159021760328 1104 8 0.0033155851576509576 1104 9 0.0334761503498342 1104 10-11 4.321518009078318E-4 1104 12-13 0.19058854433277617 1104 14-15 0.2501547534144066 1104 16-17 -0.012450033897195567 1104 18-19 0.07732288128702436 1104 20-21 0.08272661160406614 1104 22-23 0.022512022352394467 1104 24-25 0.020207251766478862 1104 26-27 0.08643929822493845 1104 28-29 -0.005967862279334213 1104 30-31 0.07438102928857404 1104 32-33 0.0845218465485047 1104 34-35 0.2415359941664903 1104 36-37 0.19518617677835692 1104 38-39 0.1219719180981258 1104 40-41 0.05420300637957354 1104 42-43 -0.10681682698741213 1104 44-45 0.020808159784294844 1104 46-47 -0.12099232547990368 1104 48-49 -0.12209802853269025 1104 50-51 -0.2551818829270829 1104 52-53 -0.2894552118470841 1104 54-55 -0.23444013638085437 1104 56-57 -0.3865208980633241 1104 58-59 -0.09185178146090323 1104 60-61 -0.18418912606562543 1104 62-63 -0.0378232530152971 1104 64-65 0.04709851130088083 1104 66-67 -0.2102391868241753 1104 68-69 -0.11033883961010105 1104 70-71 -0.27002173452569167 1104 72-73 -0.34766615999295425 1104 74-75 -0.33205450605918685 1104 76-77 -0.164035594466025 1104 78-79 -0.03495157153198747 1104 80-81 0.009781075736842837 1104 82-83 0.03382298758697644 1104 84-85 -0.13018560282108638 1104 86-87 -0.2586188919487924 1104 88-89 -0.3724361008250092 1104 90-91 -0.14376790100250858 1104 92-93 0.12192638039667614 1104 94-95 -0.10924870700021216 1104 96-97 -0.07915825070935156 1104 98-99 0.19298152635252208 1104 100-101 0.4157659284249462 1105 1 0.2148811010463234 1105 2 0.18140959409638668 1105 3 0.18070195768487451 1105 4 0.11908285139028862 1105 5 0.13368802706457927 1105 6 0.13038230615072166 1105 7 0.11694180876177995 1105 8 0.1423863604407387 1105 9 0.22124251530422612 1105 10-11 0.10672130120371293 1105 12-13 0.1674837129042146 1105 14-15 0.24884078857151337 1105 16-17 0.29297754376172236 1105 18-19 0.2743338961517523 1105 20-21 0.30013476116639737 1105 22-23 0.3686650593730718 1105 24-25 0.3437038182772909 1105 26-27 0.34389225758230424 1105 28-29 0.24922444642439245 1105 30-31 0.31885738055108703 1105 32-33 0.29682665834210553 1105 34-35 0.4326021727640281 1105 36-37 0.45901787006617667 1105 38-39 0.3787893707769783 1105 40-41 0.4599854159053365 1105 42-43 0.3597322928577569 1105 44-45 0.4150423172602231 1105 46-47 0.4096715291792776 1105 48-49 0.36196653193335493 1105 50-51 0.26777743938612275 1105 52-53 0.2439847112960578 1105 54-55 0.38700080707921103 1105 56-57 0.3226861473828535 1105 58-59 0.3409839282291287 1105 60-61 0.24279553937736154 1105 62-63 0.4091079948042591 1105 64-65 0.41512744131799906 1105 66-67 0.5838272845447179 1105 68-69 0.6040311497484439 1105 70-71 0.717245341319618 1105 72-73 0.6316325386646753 1105 74-75 0.5986038262661033 1105 76-77 0.4813122336789455 1105 78-79 0.6555820962969996 1105 80-81 0.7162330901615341 1105 82-83 0.8327929408312187 1105 84-85 0.9514248499927618 1105 86-87 0.9297588742583827 1105 88-89 0.631574603737139 1105 90-91 0.7916484040827747 1105 92-93 0.7309696842600282 1105 94-95 0.6543778710286112 1105 96-97 0.8740158640955862 1105 98-99 1.3548328055178942 1105 100-101 1.4521300588502015 1106 1 -0.0794177941411931 1106 2 -0.03438633052441986 1106 3 -0.006775699227432597 1106 4 0.01733455797660355 1106 5 -0.052982725195263924 1106 6 0.015377965207974853 1106 7 0.13527761047432563 1106 8 -0.055504452081606814 1106 9 -0.12940692377787144 1106 10-11 2.5585000101813193E-4 1106 12-13 -0.12220952714141475 1106 14-15 -0.1234350570611582 1106 16-17 -0.04832610092786638 1106 18-19 0.04219474765746156 1106 20-21 0.030796286794981143 1106 22-23 -0.03104639560880429 1106 24-25 0.02893014311599984 1106 26-27 -0.11750995204267412 1106 28-29 0.05361135209825818 1106 30-31 0.04174180933409133 1106 32-33 -0.01951060737465582 1106 34-35 0.07130806112520105 1106 36-37 -0.04677005360132824 1106 38-39 -0.06762807470588683 1106 40-41 0.07463737443656981 1106 42-43 -0.08301833274869352 1106 44-45 -0.15891713322749723 1106 46-47 -0.025756521822543732 1106 48-49 0.12825187657016102 1106 50-51 0.12345726506747923 1106 52-53 0.2566279198485617 1106 54-55 -0.21400441907539403 1106 56-57 0.03323974525834217 1106 58-59 -0.0070686056898026095 1106 60-61 -0.056449477050129815 1106 62-63 0.12687096230676786 1106 64-65 -0.11684147412723433 1106 66-67 -0.04037645036837745 1106 68-69 -0.162164822537612 1106 70-71 -0.16426224256271382 1106 72-73 0.021965854974709487 1106 74-75 -0.05895537227670289 1106 76-77 -0.14697794557645594 1106 78-79 0.15620770275223705 1106 80-81 0.08663381875897969 1106 82-83 -0.10818811223042246 1106 84-85 -0.06612958202466857 1106 86-87 0.1257388208052035 1106 88-89 0.2800748505750619 1106 90-91 0.2996536132140726 1106 92-93 0.4796503781299357 1106 94-95 0.03225012814861117 1106 96-97 0.0961172208529888 1106 98-99 0.16891363216958055 1106 100-101 -0.3546639421371509 1107 1 0.11844602428337936 1107 2 0.10914671353432936 1107 3 0.08074098969112242 1107 4 0.0867854925560465 1107 5 0.08235605985146321 1107 6 0.12608390646298062 1107 7 0.11357367188954726 1107 8 -0.017679018169722838 1107 9 -0.05126106262967767 1107 10-11 0.08549032930675793 1107 12-13 0.1196003860762147 1107 14-15 0.195632032391444 1107 16-17 0.146813251542099 1107 18-19 0.12974064218349923 1107 20-21 0.10699788893116846 1107 22-23 0.11076351760882375 1107 24-25 0.08728711908395326 1107 26-27 0.016226696822485565 1107 28-29 0.03426055103017234 1107 30-31 0.09255956634343221 1107 32-33 0.14078645537567525 1107 34-35 0.2610735818194527 1107 36-37 0.21635995307423883 1107 38-39 0.17134889458650804 1107 40-41 0.2487320006047895 1107 42-43 0.3206490571044398 1107 44-45 0.19179631804086483 1107 46-47 0.31442204960602993 1107 48-49 0.257490861883916 1107 50-51 0.40295910084852693 1107 52-53 0.15994067018234404 1107 54-55 0.344164806558787 1107 56-57 0.44215579999799814 1107 58-59 0.477723617274151 1107 60-61 0.4590336671421298 1107 62-63 0.5405767366725911 1107 64-65 0.5845645672612747 1107 66-67 0.5141128687237355 1107 68-69 0.40107366210857975 1107 70-71 0.4378030111088407 1107 72-73 0.2746986653885912 1107 74-75 0.3004196277232971 1107 76-77 0.17891908379603905 1107 78-79 0.33658570675757815 1107 80-81 0.3428838187589811 1107 82-83 0.25867603997251365 1107 84-85 0.37537993065890873 1107 86-87 0.36195377407623397 1107 88-89 0.4679044567165853 1107 90-91 0.6210029122794971 1107 92-93 0.7979038828028351 1107 94-95 0.595955902514433 1107 96-97 0.7517497308663401 1107 98-99 1.0167440727570316 1107 100-101 1.1796150965811414 1108 1 -0.21680097171128665 1108 2 -0.20074147071133552 1108 3 -0.12546728801248008 1108 4 -0.058366376602833725 1108 5 -0.19690795884012147 1108 6 -0.25097717497894223 1108 7 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2316 50-51 -0.7553803961928978 2316 52-53 -0.5491833788455551 2316 54-55 -0.5195344749289532 2316 56-57 -0.6487650579901683 2316 58-59 -0.5286611037740414 2316 60-61 -0.42415790090520744 2316 62-63 -0.32072662342884684 2316 64-65 -0.3671128418680567 2316 66-67 -0.4284801152154003 2316 68-69 -0.39032268193199116 2316 70-71 -0.6358788358903098 2316 72-73 -0.41052164314902484 2316 74-75 -0.28740000456749115 2316 76-77 -0.4351685113011712 2316 78-79 -0.3388448722787736 2316 80-81 -0.592529325788778 2316 82-83 -0.7369230724535214 2316 84-85 -0.6854373770333986 2316 86-87 -0.6821501988738916 2316 88-89 -0.6293720648387833 2316 90-91 -0.6994931781685203 2316 92-93 -0.963119468845516 2316 94-95 -0.6180524449073772 2316 96-97 -0.4466917315174328 2316 98-99 -0.2714850058566647 2316 100-101 -0.0399326633512338 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 6.0 5 22.0 6 50.0 7 107.0 8 259.0 9 412.0 10 751.0 11 1274.0 12 1899.0 13 2878.0 14 3896.0 15 5272.0 16 7233.0 17 10418.0 18 10471.0 19 6727.0 20 5320.0 21 5442.0 22 5754.0 23 5903.0 24 6619.0 25 7964.0 26 9497.0 27 11780.0 28 14120.0 29 17450.0 30 21189.0 31 26159.0 32 33447.0 33 43446.0 34 60274.0 35 93117.0 36 147328.0 37 239694.0 38 207704.0 39 21199.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01239314918152 19.01393891147419 14.930228154514399 22.04343978482989 2 15.686213883416253 20.63052504137893 39.97006610000319 23.713194975201628 3 20.005024057506134 24.741357654924542 29.796429054511027 25.457189233058298 4 12.975998176000495 17.19042245683459 36.504915524090706 33.32866384307421 5 13.553050561884595 37.71476434853848 33.572701065030216 15.159484024546705 6 31.38081013401759 36.18822747437899 17.188683474255328 15.24227891734809 7 28.38415670938123 33.35127061660458 20.1247623390475 18.13981033496669 8 26.5293957386951 34.48354816333392 19.143314249499074 19.843741848471907 9 26.353126895981386 17.270675616664928 20.355932465384452 36.02026502196923 10-11 25.28557958357164 26.776032569211505 26.300855579428994 21.637532267787858 12-13 26.489486884206947 24.27093155364568 27.228940920965172 22.0106406411822 14-15 22.985968342788247 25.523578671571883 26.45219536889617 25.0382576167437 16-17 21.757799367007895 28.923014943714715 26.958101951145995 22.3610837381314 18-19 21.53628483761767 27.085426553104664 29.895863926545374 21.48242468273229 20-21 22.587451501708067 25.720566753744613 30.09135455149707 21.600627193050254 22-23 22.199996522034844 25.860168410801787 30.18733672885784 21.752498338305536 24-25 22.179034943049793 26.07777381652846 30.126363853310107 21.61682738711164 26-27 21.785922904957214 26.26916251435573 30.345054502251738 21.599860078435313 28-29 21.391863006750636 26.77247092637096 30.314200490296717 21.52146557658169 30-31 21.679171239546783 26.103625971898044 30.233806207781367 21.98339658077381 32-33 21.472336034888023 26.322864929672306 30.187482549708573 22.017316485731094 34-35 22.21230707537256 26.27770517227635 29.905328331203485 21.604659421147606 36-37 22.05266437691115 25.93249568859325 30.132264780758323 21.882575153737278 38-39 21.625025787503244 26.431139998482944 30.069702353028145 21.874131860985667 40-41 21.873417770442888 26.404681660244343 29.966606501683202 21.755294067629563 42-43 21.74797729859459 26.41323398408892 29.579266513494662 22.259522203821827 44-45 21.838433598808958 26.345319911251565 29.582570294200195 22.23367619573928 46-47 22.213183806594223 26.854624920779685 29.06487177901782 21.86731949360827 48-49 22.08759062031441 26.79139358199496 29.024923484766514 22.096092312924117 50-51 21.88514406504568 27.104120615831697 28.99338027298162 22.017355046141006 52-53 22.28419226191396 27.257295998021426 28.385992302103784 22.072519437960832 54-55 21.845582211367535 26.95650031688127 28.776732026648943 22.42118544510225 56-57 22.140677894053454 27.079001978575732 28.592061737745972 22.188258389624842 58-59 22.48200153030467 26.757193591269534 28.51380752167932 22.24699735674648 60-61 22.00605165937582 26.918484225495803 29.075595504923253 21.999868610205123 62-63 21.78843731161022 27.08735875597051 28.912227752616204 22.21197617980307 64-65 21.600337362620376 27.255750235728748 28.719345601533398 22.42456680011748 66-67 21.748440712287266 27.179621442814522 28.417438903745385 22.654498941152827 68-69 21.703159749662344 27.047084192363503 28.63459126909752 22.61516478887663 70-71 21.877415253582306 27.12378077999165 28.41603805666765 22.582765909758397 72-73 21.914030497890035 26.946790997480406 28.590805805883175 22.548372698746384 74-75 21.663665311548392 27.63209504892338 28.146833892384027 22.557405747144205 76-77 21.80090002009491 27.10851637735149 28.286821990014378 22.803761612539226 78-79 21.879395761519792 27.341395127757252 28.286966905229317 22.49224220549364 80-81 21.940646592367024 27.4493086578146 28.175720325228387 22.434324424589985 82-83 21.850492688292235 27.296559273065586 28.374246983223138 22.47870105541904 84-85 21.818998644989723 27.334059547991757 28.322275064670944 22.524666742347573 86-87 21.90594101254535 27.32772058706318 28.087610238584386 22.67872816180708 88-89 21.755900711727612 27.350309684113512 27.88364649541536 23.010143108743517 90-91 21.738059319737353 27.267216089878566 28.120913682975946 22.87381090740813 92-93 22.053996557768954 27.12406571637388 28.17465627202417 22.647281453832996 94-95 21.76375341999907 27.003501151592907 28.229049124325662 23.003696304082357 96-97 21.580242452815607 27.057651136908166 28.34667197378387 23.015434436492356 98-99 21.712356823767642 27.172375682067614 27.876132270879385 23.239135223285363 100-101 21.579469571669268 26.880709659468565 28.237019461147266 23.302801307714898 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1358.0 1 896.0 2 751.5 3 994.0 4 673.0 5 513.5 6 989.5 7 2145.0 8 3355.0 9 3759.0 10 2980.0 11 1953.0 12 1766.0 13 1900.0 14 1953.5 15 1954.0 16 1982.5 17 2053.0 18 2137.0 19 2286.0 20 2439.0 21 2675.5 22 3097.0 23 3617.0 24 4299.0 25 5111.5 26 6049.5 27 7248.5 28 8454.5 29 9778.0 30 11589.5 31 13164.0 32 14896.0 33 16489.5 34 17800.5 35 19278.5 36 20878.5 37 22964.5 38 24702.0 39 25875.0 40 27282.0 41 29445.5 42 31478.5 43 33445.5 44 35443.5 45 37147.5 46 37774.0 47 40041.0 48 45946.0 49 45779.5 50 40781.5 51 38013.0 52 36102.5 53 35459.0 54 34526.0 55 31554.0 56 28189.0 57 25735.5 58 23884.0 59 21654.0 60 18227.0 61 14536.0 62 11183.0 63 8291.0 64 6363.5 65 5101.5 66 3863.5 67 3091.5 68 2412.0 69 1625.0 70 1130.5 71 832.0 72 670.0 73 575.0 74 472.0 75 384.0 76 242.5 77 120.5 78 68.0 79 37.0 80 18.0 81 11.0 82 9.5 83 6.0 84 3.0 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0136220302041952 3 0.006569489743867188 4 0.0 5 0.0 6 0.0 7 0.0 8 5.796608597529872E-4 9 3.8644057316865815E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.8644057316865815E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005893218740822036 26-27 0.02149575688250661 28-29 4.830507164608227E-5 30-31 0.0 32-33 4.8305071646082264E-4 34-35 0.018887283013618167 36-37 0.004154236161563075 38-39 0.01811440186728085 40-41 0.015071182353577666 42-43 0.00830847232312615 44-45 0.0026567789405345247 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.796608597529872E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.449152149382468E-4 84-85 0.003912710803332663 86-87 3.381355015225759E-4 88-89 9.661014329216454E-5 90-91 2.4152535823041132E-4 92-93 3.381355015225759E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1035088.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.89655531972546 #Duplication Level Percentage of deduplicated Percentage of total 1 83.02460435862321 51.389370165818406 2 10.089092786255694 12.489601795406372 3 2.773337125158196 5.149800443628078 4 1.225168776084941 3.033349076997675 5 0.6831086956729095 2.114103758555187 6 0.4340307323456528 1.611900434105619 7 0.29480147131125134 1.2773036904147328 8 0.23194292810994496 1.1485174624621053 9 0.1649541887066133 0.918908645984939 >10 0.976577173237352 11.04659029775622 >50 0.06675335026104752 2.7825327775260766 >100 0.032346850599303936 3.5863783228813793 >500 0.0020314441543277514 0.8954579410220489 >1k 9.375896096897315E-4 1.252944714625148 >5k 3.1252986989657716E-4 1.3032404728160605 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7211 0.6966557432797984 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6263 0.6050693274388265 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3947 0.38132023557417344 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3142 0.303549070223981 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1733 0.16742537832532114 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1723 0.16645927689239948 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1218 0.1176711545298564 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1191 0.11506268066096795 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.449152149382468E-4 0.0 14-15 0.0 0.0 0.0 1.9322028658432907E-4 0.0 16-17 0.0 0.0 0.0 2.4152535823041135E-4 0.0 18-19 0.0 0.0 0.0 2.898304298764936E-4 0.0 20-21 0.0 0.0 0.0 2.898304298764936E-4 0.0 22-23 0.0 0.0 0.0 5.796608597529872E-4 0.0 24-25 0.0 0.0 0.0 8.211862179833985E-4 0.0 26-27 0.0 0.0 0.0 0.001304236934444221 0.0 28-29 0.0 0.0 0.0 0.00405762601827091 0.0 30-31 0.0 0.0 0.0 0.01217287805481273 0.0 32-33 0.0 0.0 0.0 0.030094059635509252 0.0 34-35 0.0 0.0 0.0 0.05550252732134852 0.0 36-37 0.0 0.0 0.0 0.09622370271899588 0.0 38-39 0.0 0.0 0.0 0.16022792265005487 9.661014329216454E-5 40-41 0.0 0.0 0.0 0.22611604037531108 9.661014329216454E-5 42-43 0.0 0.0 0.0 0.2902651755213083 9.661014329216454E-5 44-45 0.0 0.0 0.0 0.37045159445380493 9.661014329216454E-5 46-47 0.0 0.0 0.0 0.4665303819578625 9.661014329216454E-5 48-49 0.0 0.0 0.0 0.5664735751936067 9.661014329216454E-5 50-51 0.0 0.0 0.0 0.669363377799762 9.661014329216454E-5 52-53 0.0 0.0 0.0 0.7707074181132425 9.661014329216454E-5 54-55 0.0 0.0 0.0 0.8809878966812483 9.661014329216454E-5 56-57 0.0 0.0 0.0 1.0080785401820909 9.661014329216454E-5 58-59 0.0 0.0 0.0 1.1460378248033019 9.661014329216454E-5 60-61 0.0 0.0 0.0 1.2950589708314655 9.661014329216454E-5 62-63 0.0 0.0 0.0 1.4401191009846506 9.661014329216454E-5 64-65 0.0 0.0 0.0 1.5829088927704698 9.661014329216454E-5 66-67 0.0 0.0 0.0 1.746856305937273 9.661014329216454E-5 68-69 0.0 0.0 0.0 1.9292079514012335 9.661014329216454E-5 70-71 0.0 0.0 0.0 2.126582474147126 1.9322028658432907E-4 72-73 0.0 0.0 0.0 2.323377336033265 1.9322028658432907E-4 74-75 0.0 0.0 0.0 2.516211182044425 1.9322028658432907E-4 76-77 0.0 0.0 0.0 2.727642480639327 1.9322028658432907E-4 78-79 0.0 0.0 0.0 2.9586373332508926 1.9322028658432907E-4 80-81 0.0 0.0 0.0 3.205572859505665 1.9322028658432907E-4 82-83 0.0 0.0 0.0 3.460237197223811 1.9322028658432907E-4 84-85 0.0 0.0 0.0 3.708670180699612 1.9322028658432907E-4 86-87 0.0 0.0 0.0 3.9715463805975917 1.9322028658432907E-4 88-89 0.0 0.0 0.0 4.258961556891782 1.9322028658432907E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGAGT 35 2.0549237E-4 33.92569 72-73 CGTATAG 60 0.003951481 31.665506 3 CGTAGAC 105 6.906905E-5 27.141863 3 TAGACTA 230 5.456968E-12 26.845547 5 GGTATCA 6600 0.0 24.11246 1 GTATCAA 8675 0.0 23.821026 1 TACATGG 5245 0.0 23.639357 2 GTACATG 5305 0.0 23.55109 1 CGTAAGA 125 2.2540383E-4 22.802471 2 GAGTACT 4935 0.0 22.761492 12-13 ACATGGG 5305 0.0 22.204653 3 TACCTGG 625 0.0 22.042387 2 AGTACTT 4940 0.0 21.776997 12-13 CCTAGAC 375 0.0 21.532545 3 GTCTATA 295 1.3278623E-10 20.934471 1 GTACTTT 5325 0.0 20.916065 14-15 CTAGACT 365 0.0 20.820148 4 GTATAGG 210 8.597035E-7 20.359348 1 CCGTTCA 140 4.8540687E-4 20.355415 9 ATCAACG 10130 0.0 20.255922 3 >>END_MODULE