FastQCFastQC Report
Thu 26 May 2016
SRR938264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990515
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67550.6819684709469316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55470.5600117110795899No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33560.3388136474460256No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28400.28671953478745904No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16590.1674886296522516No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15260.15406127115692342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11500.11610122007238657No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10620.10721695279728222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACG650.00582550529.2494138
GGTATCA61050.028.088781
GTAAGCG1450.026.20505794-95
GTATCAA82050.024.4410321
GTACATG48100.023.8664631
TACATGG47900.023.2334942
GTATAGG2303.5088306E-922.7814271
GAGTACT49250.022.43816212-13
CGCTCCG1205.973561E-921.75830848-49
ACATGGG49950.021.3278313
TACCTGG6700.021.2951452
GTACTTT51900.021.20142714-15
ATTCGCG1351.0131771E-921.09470280-81
ATCAACG95250.020.8211773
TATCAAC96300.020.7423132
TCAACGC96450.020.6114334
CAACGCA96500.020.5514725
TACACCG1404.8105267E-420.3824985
CAATTCG1401.5952537E-920.3382478-79
AACGCAG98850.020.0588326
Produced by FastQC (version 0.11.5)